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The map label for this gene is phoS2

Identifier: 221230609

GI number: 221230609

Start: 2490990

End: 2492099

Strand: Reverse

Name: phoS2

Synonym: MLBr_02095

Alternate gene names: 221230609

Gene position: 2492099-2490990 (Counterclockwise)

Preceding gene: 221230611

Following gene: 221230608

Centisome position: 76.26

GC content: 57.03

Gene sequence:

>1110_bases
TTGAAGCTCAACCAGTTTGGTGCCGCGATTGGGCTCCTGGCTACTGGTGCGCTGTTGTCGGGCTGCGGCAGCGACAACAA
CGCAGCTGTAGGAAGTGCACGGACAGGCCCATCGTCAGGCCAGGTGAGCTGCGGTGGGAAGCCGACACTGAAAGCTAGCG
GGTCGACAGCCCAGGCCAACGCGATGACCCGCTTTGTTAATGCGTTCGAACGGTCATGTCCAGGACAGACGTTGAACTAT
ACGGCTAATGGTTCCGGTGCGGGGGTTAGCGAATTCAACGGTAATCAAACAGATTTCGGTGGCTCAGACTCACCGTTGAG
TAGGAAGGAGTATGCCGCAGCAGAGCAACGTTGTGGCTCGCAGGCGTGGAATTTACCGGTGGTCTTCGGCCCCATCGCGA
TCACTTACAACGTGAACGGCCTGAGTTCACTGAACCTGGACGGTCCGACAACCGCGAAGATCTTCAATGGTTCCATCGCC
AGTTGGAACGATCCCGCGATCCAAGCGCTGAACACTGGCGTCGCCCTTCCGGCTGAGCCGATTCATGTCGTGTTCCGCAA
CGACGAGTCCGGTACCACGGATAATTTCCAGAGGTACCTTGACGTCGCATCAAACGGTGAGTGGGGCAAAGGTATCGGCA
AGACCTTTAAAGGCGGTGTTGGTGAGGGTGCCAAGGGCAATGACGGTACGTCGGCGGCCGTCAAGTCCACTGAAGGGTCG
ATCACCTACAACGAATGGTCGTTCGCTTCGGCGCGGAAGCTGAACACGGCCAAGATCGCCACGTCGGCCGATCCAGAACC
CATTGCGATCAGCGTCGACTCGGTCGGCAAGACAATTTCTGGGGCTACGATCATTGGAGAGGGTAACGATTTAGTGCTTG
ACACGGTCTCGTTTTACAAGCCGGCTCAGCCCGGCTCCTACCCAATCGTGCTGGCGACCTACGAGATTGTCTGCTCGAAG
TATCCCGATGCCCAGGTCGGTAGGGCTGTGAAGGCATTCCTGCAGAGCACCATCGGTGGAGGCCAGAATGGCCTAGGGGA
CAACGGATATGTACCTATTCCGGATTCTTTCAAATCCAGATTGTCGACTGCGGCTAATGCCATCGCTTGA

Upstream 100 bases:

>100_bases
AAACCTTGTTAATGTAGCCTCATGACTCCGGATAAGACGGCTAGTTGGAGCGCCGGCTCTACTGGGGGCAGTCAGATTTA
CGCTACCGTGAGGAGCTAGA

Downstream 100 bases:

>100_bases
TATGACATCGTCACGGCGACAAATTCGTTAGTACCTGCGACTGTGATAAAGCCCACGCCAGCAGCAGTCGGTTCATATGT
AACCCGGCGGTGCGATCGGC

Product: PstS component of phosphate uptake

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 3; PstS-3

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKLNQFGAAIGLLATGALLSGCGSDNNAAVGSARTGPSSGQVSCGGKPTLKASGSTAQANAMTRFVNAFERSCPGQTLNY
TANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIA
SWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGS
ITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSK
YPDAQVGRAVKAFLQSTIGGGQNGLGDNGYVPIPDSFKSRLSTAANAIA

Sequences:

>Translated_369_residues
MKLNQFGAAIGLLATGALLSGCGSDNNAAVGSARTGPSSGQVSCGGKPTLKASGSTAQANAMTRFVNAFERSCPGQTLNY
TANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIA
SWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGS
ITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSK
YPDAQVGRAVKAFLQSTIGGGQNGLGDNGYVPIPDSFKSRLSTAANAIA
>Mature_369_residues
MKLNQFGAAIGLLATGALLSGCGSDNNAAVGSARTGPSSGQVSCGGKPTLKASGSTAQANAMTRFVNAFERSCPGQTLNY
TANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIA
SWNDPAIQALNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGS
ITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYKPAQPGSYPIVLATYEIVCSK
YPDAQVGRAVKAFLQSTIGGGQNGLGDNGYVPIPDSFKSRLSTAANAIA

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family

Homologues:

Organism=Escherichia coli, GI2367271, Length=328, Percent_Identity=31.0975609756098, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PSTS3_MYCLE (Q9CBE5)

Other databases:

- EMBL:   AL583924
- PIR:   B87171
- RefSeq:   NP_302395.1
- ProteinModelPortal:   Q9CBE5
- SMR:   Q9CBE5
- EnsemblBacteria:   EBMYCT00000029331
- GeneID:   908711
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2095
- NMPDR:   fig|272631.1.peg.1267
- Leproma:   ML2095
- GeneTree:   EBGT00050000017160
- HOGENOM:   HBG428828
- OMA:   INIPVYI
- ProtClustDB:   CLSK799391
- BioCyc:   MLEP272631:ML2095-MONOMER
- InterPro:   IPR005673
- InterPro:   IPR006059
- TIGRFAMs:   TIGR00975

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 37733; Mature: 37733

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNQFGAAIGLLATGALLSGCGSDNNAAVGSARTGPSSGQVSCGGKPTLKASGSTAQAN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCHHHH
AMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
QAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQALNTGVALPAEP
CCCCCCEEEEEEEEEEECCCCEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCC
IHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGS
EEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCC
ITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYK
EEECCCCCCHHHCCCCEEEECCCCCCCEEEEHHCCCCEECCEEEEECCCCEEEEEHHHCC
PAQPGSYPIVLATYEIVCSKYPDAQVGRAVKAFLQSTIGGGQNGLGDNGYVPIPDSFKSR
CCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHH
LSTAANAIA
HHHHHHHCC
>Mature Secondary Structure
MKLNQFGAAIGLLATGALLSGCGSDNNAAVGSARTGPSSGQVSCGGKPTLKASGSTAQAN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCHHHH
AMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRKEYAAAEQRCGS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
QAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQALNTGVALPAEP
CCCCCCEEEEEEEEEEECCCCEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCC
IHVVFRNDESGTTDNFQRYLDVASNGEWGKGIGKTFKGGVGEGAKGNDGTSAAVKSTEGS
EEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCC
ITYNEWSFASARKLNTAKIATSADPEPIAISVDSVGKTISGATIIGEGNDLVLDTVSFYK
EEECCCCCCHHHCCCCEEEECCCCCCCEEEEHHCCCCEECCEEEEECCCCEEEEEHHHCC
PAQPGSYPIVLATYEIVCSKYPDAQVGRAVKAFLQSTIGGGQNGLGDNGYVPIPDSFKSR
CCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHH
LSTAANAIA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11234002