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The map label for this gene is rpoA

Identifier: 221230536

GI number: 221230536

Start: 2344631

End: 2345674

Strand: Reverse

Name: rpoA

Synonym: MLBr_01957

Alternate gene names: 221230536

Gene position: 2345674-2344631 (Counterclockwise)

Preceding gene: 221230537

Following gene: 221230535

Centisome position: 71.78

GC content: 61.02

Gene sequence:

>1044_bases
ATGCTGATTTCACAACGCCCCACCTTGTCCGAGGACATCCTTACCGACAACCGGTCCCAGTTCGTCATCGAACCGTTGGA
GCCGGGATTCGGTTATACCCTGGGCAACTCGCTGCGGCGCACGCTGCTGTCGTCGATTCCCGGAGCGGCCGTTACCAGCA
TTCGTATCGACGGCGTCTTGCACGAGTTCACCACGGTGCCTGGGGTTAAGGAGGATGTCACTGAGATCATCCTGAACCTC
AAGGGTCTGGTGGTGTCCTCGGAGGAGGACGAACCGGTCACGATGTACCTGCGCAAGCAGGGTCCTGGTGAGGTCACTGC
CGGTGATATCGTGCCGCCGGCCGGCGTCACCCTGCACAATCCTGGTATGCGCATCGCGACGCTAAACGACAAAGGTAAGA
TCGAAGCGGAGCTCGTTGTCGAGCGTGGCCGCGGTTACGTGCCGGCGGTGCAAAATCGGGCTTTGGGTGCCGAAATCGGA
CGCATCCCAGTCGATTCCATCTACTCACCGGTGCTGAAGGTAACATACAAGGTGGATGCCACTCGGGTGGAGCAGCGGAC
CGACTTCGACAAGCTGATCCTCGACGTGGAGACCAAGAGTTCGATCACCCCTCGCGACGCACTAGCGTCAGCGGGTAAGA
CGCTGGTCGAGTTGTTCGGTCTGGCACGCGAGCTCAATGTCGAGGCCGAAGGAATTGAGATCGGGCCGTCTCCGGCCGAG
GCCGACCACATCGCGTCGTTCGCGCTGCCGATCGACGACCTGGATCTAACCGTGCGGTCCTACAACTGCCTCAAGCGCGA
GGGTGTGCACACCGTTGGCGAGCTGGTGTCGCGTACCGAGTCCGACCTGCTCGACATCCGCAACTTTGGGCAGAAGTCCA
TCGACGAGGTGAAGGTCAAACTGCACCAACTGGGTCTGTCGCTCAAGGACAGCCCGGACAGTTTCGACCCGTCCGAGGTC
GCCGGATACGACGTCACCACCGGCACGTGGTCCACCGACGGCGCCTACGACAGCCAGGACTACGCCGAAACCGAACAGCT
CTAA

Upstream 100 bases:

>100_bases
GTACTACTCGAAGTAGAGTGCAGACTCCCGCCGCTGCATCCGCACCGGCGGGAAACCTAACGGCATCAAATAGTGGGTGC
CGAGAAGGAGAAAGAACCTG

Downstream 100 bases:

>100_bases
CAGAACAGACAGGAGTGTCAGCAATGCCCAAGCCCACCAAGGGCCCTCGCCTCGGTGGCTCGTCTTCGCACCAAAAGGCC
CTTCTGACCAACCTGGCCGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLISQRPTLSEDILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIRIDGVLHEFTTVPGVKEDVTEIILNL
KGLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTLHNPGMRIATLNDKGKIEAELVVERGRGYVPAVQNRALGAEIG
RIPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVELFGLARELNVEAEGIEIGPSPAE
ADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVSRTESDLLDIRNFGQKSIDEVKVKLHQLGLSLKDSPDSFDPSEV
AGYDVTTGTWSTDGAYDSQDYAETEQL

Sequences:

>Translated_347_residues
MLISQRPTLSEDILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIRIDGVLHEFTTVPGVKEDVTEIILNL
KGLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTLHNPGMRIATLNDKGKIEAELVVERGRGYVPAVQNRALGAEIG
RIPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVELFGLARELNVEAEGIEIGPSPAE
ADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVSRTESDLLDIRNFGQKSIDEVKVKLHQLGLSLKDSPDSFDPSEV
AGYDVTTGTWSTDGAYDSQDYAETEQL
>Mature_347_residues
MLISQRPTLSEDILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIRIDGVLHEFTTVPGVKEDVTEIILNL
KGLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTLHNPGMRIATLNDKGKIEAELVVERGRGYVPAVQNRALGAEIG
RIPVDSIYSPVLKVTYKVDATRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVELFGLARELNVEAEGIEIGPSPAE
ADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVSRTESDLLDIRNFGQKSIDEVKVKLHQLGLSLKDSPDSFDPSEV
AGYDVTTGTWSTDGAYDSQDYAETEQL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=312, Percent_Identity=44.8717948717949, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MYCLE (Q9X798)

Other databases:

- EMBL:   AL049491
- EMBL:   AL583923
- PIR:   H87153
- RefSeq:   NP_302322.1
- ProteinModelPortal:   Q9X798
- EnsemblBacteria:   EBMYCT00000029371
- GeneID:   909427
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1957
- NMPDR:   fig|272631.1.peg.1194
- Leproma:   ML1957
- GeneTree:   EBGT00050000016135
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   MLEP272631:ML1957-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37703; Mature: 37703

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISQRPTLSEDILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIRIDGVL
CCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHH
HEFTTVPGVKEDVTEIILNLKGLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTLHN
HHHHCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEC
PGMRIATLNDKGKIEAELVVERGRGYVPAVQNRALGAEIGRIPVDSIYSPVLKVTYKVDA
CCEEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHEEEEEECH
TRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVELFGLARELNVEAEGIEIGPSPAE
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
ADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVSRTESDLLDIRNFGQKSIDEVKVK
HHHHHHHCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LHQLGLSLKDSPDSFDPSEVAGYDVTTGTWSTDGAYDSQDYAETEQL
HHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MLISQRPTLSEDILTDNRSQFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSIRIDGVL
CCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHH
HEFTTVPGVKEDVTEIILNLKGLVVSSEEDEPVTMYLRKQGPGEVTAGDIVPPAGVTLHN
HHHHCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEC
PGMRIATLNDKGKIEAELVVERGRGYVPAVQNRALGAEIGRIPVDSIYSPVLKVTYKVDA
CCEEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHEEEEEECH
TRVEQRTDFDKLILDVETKSSITPRDALASAGKTLVELFGLARELNVEAEGIEIGPSPAE
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
ADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELVSRTESDLLDIRNFGQKSIDEVKVK
HHHHHHHCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LHQLGLSLKDSPDSFDPSEVAGYDVTTGTWSTDGAYDSQDYAETEQL
HHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002