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The map label for this gene is sppA [H]

Identifier: 221230459

GI number: 221230459

Start: 2226861

End: 2228669

Strand: Reverse

Name: sppA [H]

Synonym: MLBr_01839

Alternate gene names: 221230459

Gene position: 2228669-2226861 (Counterclockwise)

Preceding gene: 221230460

Following gene: 221230458

Centisome position: 68.2

GC content: 62.3

Gene sequence:

>1809_bases
ATGTCCGCCTTTCTGCCCCCTTTTCCTGGTATCGATGACGTGCGTGCCCTGGCCAACCGGGTTAACACGGCCCGTCACCA
TGGCATACCGAATGGTTGCGTGCTCGAAGTCAATCTGCGGTCGTTGCCGCCGGAAACGACCAGCTTTGACCCCTTCACAA
TTATCACTAGGGGTGGCCGACCGATGGCCCTGCGCGAGGCTGTCGCCGCGATTCACCGCGCCGCCGCCGACCCCCGGGTC
GCCGGTATGATTGCCCGCGTTCAGCTTGGTGCGTCGCCGCCTGCGGCAGTGCAGGAACTGCGCGAAGCCGTCGCCACTTT
CAGTGCGGTCAAGCCGTCACTGGCCTGGGCCGAGACTTATCCTGGCACGCTGTCCTACTACCTGGCTTCGGCCTTTGGTG
AGGTCTGGATGCAGCCGTCGGGTAGTGTCGGGCTGGTCGGCTTCGCTAGCAATGCCACGTTTCTACGCGACGTGCTGGCC
AAGGTAGGGATCGAAGCGCAGTTCGTCGCACGGGGTGAATACAAGTCAGCGGCAAACCTTTTCACCGAAGGCGGCTTCAC
CGACGCTCACCGCGAGGCGGTCAGCCGAATGCTGGAAAGTCTGCAAGGCCAGGTGTGGCAGGGGATCGCCGAATCACGCA
GGATCGATCCCGCAGCGCTCAATCCGTTGGCCGACCGGGCTCCTCTGTTACGCGATGACGCGGTCGCTTCCGGTTTGGTC
GACCGTATTGGATTCCGCGACGAAGCTTATGCTCGTATAGCGGAACTTGTTGGTGCTGAAAGTATTACACCAAATTCAAG
CGAGCCGCAGGCGAGAACAGAGGCCAACGACGGTCTACCGCGACTGTATTTGTCGCGCTACGCTAGTGCGGGCCGGTCAC
GGTTGATGCCCCCCATGCCGTTGATTCCCGGACGCCGATCCAAGCCAACGGTTGTCGTGGTCACTCTGGAAGGTCCGATT
GTCAACGGGCGCGGTGGACCGCAGTTTCTGCCGTTCGGTATCTCTACCGGCGGTGGTGACACCATTGCGGCGGCGCTCCG
CGAGGTCGCTGCCGACGATTCAGTGTCCGCGATTGTGCTGCGGGTGAACAGCCCGGGTGGCTCGGTTACCGCATCTGAAA
CCATCTGGCGTGAGGTGAAAAGGGCCCGCGATCGCGGCAAGCCGGTAGTGGTTTCCATGGGGGCGGTAGCTGCCTCTGGC
GGCTACTACGTCTCGGTGGGCGCTGACGAGATTGTGGCCAATCCCGGTACGATCACCGGTTCGATTGGCGTGATCACCGG
GAAGCTGGTGATTCGGGACCTGAAAGGTCGGTTGGGCGTTGGGTCAGATACGGTGCGCACCAACGCCAATGCTGATGCGT
GGTCGGCTAACGTGCCGTTCACGCCGGAGCAGCACACCCATCGAGAGGCCGAGGCGGACCTGTGCTATGCTGACTTTCTT
GAACGCGTAGCAGAAGGCCGCGGCATGACCACTGATGCAGTGGATGATGTTGCGCGAGGGCGGATTTGGACTGGTGCCGA
TGCTCTCGAACGCGGTCTGGTCGACGAACTCGGCGGGCTTGTAACCGCGGTGCGCAGAGCCAAGGTCCTAGCCGGTTTGG
ATGCGGACGTCGATGTCCGCATCGTCAGTTACCCCGGCTCGTCGTTGCTGGACATGGTACGACCCCGTGCGTCGTCGCAA
CCCGGGGCTGCGTCGTTGCCAGACGCGGTAGCCACGCTGCTAGGACGCTCGGTTGCCGGGATACTCGAACAGGTCGAGCA
AGCGTTAAGCGGTGTCAGAGTGCTGTGGTTGGGGGAGTACCGGTTTTGA

Upstream 100 bases:

>100_bases
GTGATCGAGAACTTCACTTCACCACTGTACGGTGGGTGGCTTCACCACGGCTGAGCAGCGGTCTGAGATGTCCGACGCCA
CGTATCGGTACGCTGCAAGC

Downstream 100 bases:

>100_bases
GAGTCTTAGCGATGTGGCTCATCTCGTTGAAAACGGTTAGTCAGAGTGACGGGGCGACGATCTGTGACACTTCTCCAAAT
GGAGTTACTTTCGACAGCTA

Product: protease IV, signal peptide peptidase

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSAFLPPFPGIDDVRALANRVNTARHHGIPNGCVLEVNLRSLPPETTSFDPFTIITRGGRPMALREAVAAIHRAAADPRV
AGMIARVQLGASPPAAVQELREAVATFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLVGFASNATFLRDVLA
KVGIEAQFVARGEYKSAANLFTEGGFTDAHREAVSRMLESLQGQVWQGIAESRRIDPAALNPLADRAPLLRDDAVASGLV
DRIGFRDEAYARIAELVGAESITPNSSEPQARTEANDGLPRLYLSRYASAGRSRLMPPMPLIPGRRSKPTVVVVTLEGPI
VNGRGGPQFLPFGISTGGGDTIAAALREVAADDSVSAIVLRVNSPGGSVTASETIWREVKRARDRGKPVVVSMGAVAASG
GYYVSVGADEIVANPGTITGSIGVITGKLVIRDLKGRLGVGSDTVRTNANADAWSANVPFTPEQHTHREAEADLCYADFL
ERVAEGRGMTTDAVDDVARGRIWTGADALERGLVDELGGLVTAVRRAKVLAGLDADVDVRIVSYPGSSLLDMVRPRASSQ
PGAASLPDAVATLLGRSVAGILEQVEQALSGVRVLWLGEYRF

Sequences:

>Translated_602_residues
MSAFLPPFPGIDDVRALANRVNTARHHGIPNGCVLEVNLRSLPPETTSFDPFTIITRGGRPMALREAVAAIHRAAADPRV
AGMIARVQLGASPPAAVQELREAVATFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLVGFASNATFLRDVLA
KVGIEAQFVARGEYKSAANLFTEGGFTDAHREAVSRMLESLQGQVWQGIAESRRIDPAALNPLADRAPLLRDDAVASGLV
DRIGFRDEAYARIAELVGAESITPNSSEPQARTEANDGLPRLYLSRYASAGRSRLMPPMPLIPGRRSKPTVVVVTLEGPI
VNGRGGPQFLPFGISTGGGDTIAAALREVAADDSVSAIVLRVNSPGGSVTASETIWREVKRARDRGKPVVVSMGAVAASG
GYYVSVGADEIVANPGTITGSIGVITGKLVIRDLKGRLGVGSDTVRTNANADAWSANVPFTPEQHTHREAEADLCYADFL
ERVAEGRGMTTDAVDDVARGRIWTGADALERGLVDELGGLVTAVRRAKVLAGLDADVDVRIVSYPGSSLLDMVRPRASSQ
PGAASLPDAVATLLGRSVAGILEQVEQALSGVRVLWLGEYRF
>Mature_601_residues
SAFLPPFPGIDDVRALANRVNTARHHGIPNGCVLEVNLRSLPPETTSFDPFTIITRGGRPMALREAVAAIHRAAADPRVA
GMIARVQLGASPPAAVQELREAVATFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLVGFASNATFLRDVLAK
VGIEAQFVARGEYKSAANLFTEGGFTDAHREAVSRMLESLQGQVWQGIAESRRIDPAALNPLADRAPLLRDDAVASGLVD
RIGFRDEAYARIAELVGAESITPNSSEPQARTEANDGLPRLYLSRYASAGRSRLMPPMPLIPGRRSKPTVVVVTLEGPIV
NGRGGPQFLPFGISTGGGDTIAAALREVAADDSVSAIVLRVNSPGGSVTASETIWREVKRARDRGKPVVVSMGAVAASGG
YYVSVGADEIVANPGTITGSIGVITGKLVIRDLKGRLGVGSDTVRTNANADAWSANVPFTPEQHTHREAEADLCYADFLE
RVAEGRGMTTDAVDDVARGRIWTGADALERGLVDELGGLVTAVRRAKVLAGLDADVDVRIVSYPGSSLLDMVRPRASSQP
GAASLPDAVATLLGRSVAGILEQVEQALSGVRVLWLGEYRF

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=533, Percent_Identity=33.2082551594747, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787527, Length=167, Percent_Identity=32.3353293413174, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 63560; Mature: 63429

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFLPPFPGIDDVRALANRVNTARHHGIPNGCVLEVNLRSLPPETTSFDPFTIITRGGR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCC
PMALREAVAAIHRAAADPRVAGMIARVQLGASPPAAVQELREAVATFSAVKPSLAWAETY
CHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCC
PGTLSYYLASAFGEVWMQPSGSVGLVGFASNATFLRDVLAKVGIEAQFVARGEYKSAANL
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHH
FTEGGFTDAHREAVSRMLESLQGQVWQGIAESRRIDPAALNPLADRAPLLRDDAVASGLV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHCCCCHHHHHHHHHHH
DRIGFRDEAYARIAELVGAESITPNSSEPQARTEANDGLPRLYLSRYASAGRSRLMPPMP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCHHCCCCCCC
LIPGRRSKPTVVVVTLEGPIVNGRGGPQFLPFGISTGGGDTIAAALREVAADDSVSAIVL
CCCCCCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
RVNSPGGSVTASETIWREVKRARDRGKPVVVSMGAVAASGGYYVSVGADEIVANPGTITG
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEEECCHHHCCCCCCEEC
SIGVITGKLVIRDLKGRLGVGSDTVRTNANADAWSANVPFTPEQHTHREAEADLCYADFL
CHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
ERVAEGRGMTTDAVDDVARGRIWTGADALERGLVDELGGLVTAVRRAKVLAGLDADVDVR
HHHHCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IVSYPGSSLLDMVRPRASSQPGAASLPDAVATLLGRSVAGILEQVEQALSGVRVLWLGEY
EEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RF
CC
>Mature Secondary Structure 
SAFLPPFPGIDDVRALANRVNTARHHGIPNGCVLEVNLRSLPPETTSFDPFTIITRGGR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCC
PMALREAVAAIHRAAADPRVAGMIARVQLGASPPAAVQELREAVATFSAVKPSLAWAETY
CHHHHHHHHHHHHHCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCC
PGTLSYYLASAFGEVWMQPSGSVGLVGFASNATFLRDVLAKVGIEAQFVARGEYKSAANL
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHH
FTEGGFTDAHREAVSRMLESLQGQVWQGIAESRRIDPAALNPLADRAPLLRDDAVASGLV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHCCCCHHHHHHHHHHH
DRIGFRDEAYARIAELVGAESITPNSSEPQARTEANDGLPRLYLSRYASAGRSRLMPPMP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCHHCCCCCCC
LIPGRRSKPTVVVVTLEGPIVNGRGGPQFLPFGISTGGGDTIAAALREVAADDSVSAIVL
CCCCCCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
RVNSPGGSVTASETIWREVKRARDRGKPVVVSMGAVAASGGYYVSVGADEIVANPGTITG
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEEECCHHHCCCCCCEEC
SIGVITGKLVIRDLKGRLGVGSDTVRTNANADAWSANVPFTPEQHTHREAEADLCYADFL
CHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
ERVAEGRGMTTDAVDDVARGRIWTGADALERGLVDELGGLVTAVRRAKVLAGLDADVDVR
HHHHCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IVSYPGSSLLDMVRPRASSQPGAASLPDAVATLLGRSVAGILEQVEQALSGVRVLWLGEY
EEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
RF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]