Click here to switch to the map view.

The map label for this gene is sdaA [H]

Identifier: 221230417

GI number: 221230417

Start: 2129584

End: 2130960

Strand: Reverse

Name: sdaA [H]

Synonym: MLBr_01755

Alternate gene names: 221230417

Gene position: 2130960-2129584 (Counterclockwise)

Preceding gene: 221230419

Following gene: 221230416

Centisome position: 65.21

GC content: 60.71

Gene sequence:

>1377_bases
ATGACCATCAGCGTGTTCGATCTGTTTACGATCGGCATAGGGCCGTCCAGCTCACACACGGTCGGTCCAATGCGCGCCGC
AGCACGTTTCGTCGATGGCTTAGCTGCCAACGGTTTGCTCGAGGACGTGTGCGACATCCGTGTCGAGTTGTTCGGATCGC
TGGCGGCAACTGGGGCAGGCCACGGCACCATGTCGGCTATCCTGTTAGGGCTTGAGGGGGAGCATCCCGAGAGGCTTGAT
GCCGATGACATGGTGACCCGATTGGCCTGTATGCGCGCTGCCGGGACAATCTGTCTGGGTGGATTGATCGTGATCAAGTT
CACCGAAGCCGATGTCGTACTGCGTCCACAGACGGTGCTGGACCGGCATCCCAACGCCATGACGATCGTTGCTCTCGCCC
GCGACGGAACCAAGCTCGCCGCCGAGACTTATTATTCGGTCGGTGGCGGATTTGTCGTGGCACACGGCGATCCACCCGAG
GGCGCGGAATGGACCACTGGTGGGGTGTCCTTTGGCTCGGCGGGTGAGTTGTTAGAATTGGCGGCCACGCGAGGAATGTC
AATCTGCGAGTTGATGGCCGAGTACGAGCAAAGCCTGAGGTCCGAGCAGGAGGTACGTGCCGGTCTGCTAAATATTCGTG
ACGTCATGACGCAATCCGTTCAGCGTGGTATTGCTCAGGGCGGCTACCTGCCTGGTGGGCTGAAAGTGCGTCGCCGCGCA
CATTCCTGGCACGAGCGGCTGTGTGCCGAGGATCCGAATCGCGATCCAGTGTTCGCCGAGGACTGGGTGAACCTGGTCGC
GCTGGCAGTCAACGAGGAGAATGCCGTGGGCGGAAGAGTGGTCACCGCTCCGACTAACGGTGCGGCGGGCATCATTCCCG
CTGTTTTGCATTATGCACAGCATTACTGTCCCGCTGGGCGGGCAGACCCAGACGATACAAGTGTGCGGTTTTTGTTAACT
GCGGGGGCAATTGGTTCGCTGTACAAGGAGCTTGCGTCTATCTCCGGCGCGGAGGTCGGTTGCCAAGGTGAGGTGGGGTC
GGCGGCTTCTATGGCCGCTGCGGGCTTGGCTGAAATCCTTGGTGGCACACCGGCTCAGGTCGAGAACGCGGCTGAGATTG
CGATGGAACACAGTCTCGGTTTAACCTGTGACCCGATCGCCGGTCTGGTCCAGATTCCGTGCATCGAACGCAATGCCATC
TCGGCCGGCAAAGCGATCAATGCCGCTCGGATGGCTATGCGTGGCGACGGTACCCACCGGGTGAGCCTTGACGAGGTGAT
CGCCACCATGAGGGCAACCGGCCGGGACATGAATTTCAAGTACAAGGAGACTGCCGCGGGCGGACTCGCCCTCGCGGTCA
ACATCGTCGAATGCTGA

Upstream 100 bases:

>100_bases
TTTTCACCAGCAGTGGCCAGACGGGCGGAGGTTGCCGATAAGCGTTGTCAACGTATGGGGGGATGATGGCTGCTCGACCT
GAGACATCCGAGGCTTCATC

Downstream 100 bases:

>100_bases
CCGGCCGACGCGGTTGCCGGCGGAAGTTCGGGTGCGTAATCGCCGGTGGCAACCGACACACCCATCAATTCGCTGTATTG
ATCAACCCCACGTCGCCGGT

Product: L-serine dehydratase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTISVFDLFTIGIGPSSSHTVGPMRAAARFVDGLAANGLLEDVCDIRVELFGSLAATGAGHGTMSAILLGLEGEHPERLD
ADDMVTRLACMRAAGTICLGGLIVIKFTEADVVLRPQTVLDRHPNAMTIVALARDGTKLAAETYYSVGGGFVVAHGDPPE
GAEWTTGGVSFGSAGELLELAATRGMSICELMAEYEQSLRSEQEVRAGLLNIRDVMTQSVQRGIAQGGYLPGGLKVRRRA
HSWHERLCAEDPNRDPVFAEDWVNLVALAVNEENAVGGRVVTAPTNGAAGIIPAVLHYAQHYCPAGRADPDDTSVRFLLT
AGAIGSLYKELASISGAEVGCQGEVGSAASMAAAGLAEILGGTPAQVENAAEIAMEHSLGLTCDPIAGLVQIPCIERNAI
SAGKAINAARMAMRGDGTHRVSLDEVIATMRATGRDMNFKYKETAAGGLALAVNIVEC

Sequences:

>Translated_458_residues
MTISVFDLFTIGIGPSSSHTVGPMRAAARFVDGLAANGLLEDVCDIRVELFGSLAATGAGHGTMSAILLGLEGEHPERLD
ADDMVTRLACMRAAGTICLGGLIVIKFTEADVVLRPQTVLDRHPNAMTIVALARDGTKLAAETYYSVGGGFVVAHGDPPE
GAEWTTGGVSFGSAGELLELAATRGMSICELMAEYEQSLRSEQEVRAGLLNIRDVMTQSVQRGIAQGGYLPGGLKVRRRA
HSWHERLCAEDPNRDPVFAEDWVNLVALAVNEENAVGGRVVTAPTNGAAGIIPAVLHYAQHYCPAGRADPDDTSVRFLLT
AGAIGSLYKELASISGAEVGCQGEVGSAASMAAAGLAEILGGTPAQVENAAEIAMEHSLGLTCDPIAGLVQIPCIERNAI
SAGKAINAARMAMRGDGTHRVSLDEVIATMRATGRDMNFKYKETAAGGLALAVNIVEC
>Mature_457_residues
TISVFDLFTIGIGPSSSHTVGPMRAAARFVDGLAANGLLEDVCDIRVELFGSLAATGAGHGTMSAILLGLEGEHPERLDA
DDMVTRLACMRAAGTICLGGLIVIKFTEADVVLRPQTVLDRHPNAMTIVALARDGTKLAAETYYSVGGGFVVAHGDPPEG
AEWTTGGVSFGSAGELLELAATRGMSICELMAEYEQSLRSEQEVRAGLLNIRDVMTQSVQRGIAQGGYLPGGLKVRRRAH
SWHERLCAEDPNRDPVFAEDWVNLVALAVNEENAVGGRVVTAPTNGAAGIIPAVLHYAQHYCPAGRADPDDTSVRFLLTA
GAIGSLYKELASISGAEVGCQGEVGSAASMAAAGLAEILGGTPAQVENAAEIAMEHSLGLTCDPIAGLVQIPCIERNAIS
AGKAINAARMAMRGDGTHRVSLDEVIATMRATGRDMNFKYKETAAGGLALAVNIVEC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI48994925, Length=456, Percent_Identity=53.0701754385965, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1789161, Length=462, Percent_Identity=51.2987012987013, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1788116, Length=455, Percent_Identity=49.8901098901099, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 47780; Mature: 47649

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISVFDLFTIGIGPSSSHTVGPMRAAARFVDGLAANGLLEDVCDIRVELFGSLAATGAG
CEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
HGTMSAILLGLEGEHPERLDADDMVTRLACMRAAGTICLGGLIVIKFTEADVVLRPQTVL
CCCEEHEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHEECCEEEEEEECCCEEECCHHHH
DRHPNAMTIVALARDGTKLAAETYYSVGGGFVVAHGDPPEGAEWTTGGVSFGSAGELLEL
HCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
AATRGMSICELMAEYEQSLRSEQEVRAGLLNIRDVMTQSVQRGIAQGGYLPGGLKVRRRA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
HSWHERLCAEDPNRDPVFAEDWVNLVALAVNEENAVGGRVVTAPTNGAAGIIPAVLHYAQ
HHHHHHHHCCCCCCCCCCHHHHHEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
HYCPAGRADPDDTSVRFLLTAGAIGSLYKELASISGAEVGCQGEVGSAASMAAAGLAEIL
HCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGTPAQVENAAEIAMEHSLGLTCDPIAGLVQIPCIERNAISAGKAINAARMAMRGDGTHR
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
VSLDEVIATMRATGRDMNFKYKETAAGGLALAVNIVEC
EEHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
TISVFDLFTIGIGPSSSHTVGPMRAAARFVDGLAANGLLEDVCDIRVELFGSLAATGAG
EEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
HGTMSAILLGLEGEHPERLDADDMVTRLACMRAAGTICLGGLIVIKFTEADVVLRPQTVL
CCCEEHEEEECCCCCCCCCCHHHHHHHHHHHHHHCHHEECCEEEEEEECCCEEECCHHHH
DRHPNAMTIVALARDGTKLAAETYYSVGGGFVVAHGDPPEGAEWTTGGVSFGSAGELLEL
HCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
AATRGMSICELMAEYEQSLRSEQEVRAGLLNIRDVMTQSVQRGIAQGGYLPGGLKVRRRA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
HSWHERLCAEDPNRDPVFAEDWVNLVALAVNEENAVGGRVVTAPTNGAAGIIPAVLHYAQ
HHHHHHHHCCCCCCCCCCHHHHHEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
HYCPAGRADPDDTSVRFLLTAGAIGSLYKELASISGAEVGCQGEVGSAASMAAAGLAEIL
HCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGTPAQVENAAEIAMEHSLGLTCDPIAGLVQIPCIERNAISAGKAINAARMAMRGDGTHR
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
VSLDEVIATMRATGRDMNFKYKETAAGGLALAVNIVEC
EEHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]