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The map label for this gene is wcaL [C]

Identifier: 221230396

GI number: 221230396

Start: 2068468

End: 2069784

Strand: Direct

Name: wcaL [C]

Synonym: MLBr_01715

Alternate gene names: 221230396

Gene position: 2068468-2069784 (Clockwise)

Preceding gene: 221230395

Following gene: 221230399

Centisome position: 63.29

GC content: 61.12

Gene sequence:

>1317_bases
GTGTTTATACAGCGACACGCCGTCGTGCGTGGCATTTTTGGGACGTTCGCGCCAGCGGAGGGGACAACGCTCATGAAAAT
CCTGATAGTGTCCTGGGAATATCCGCCAGTGGTGATCGGCGGGCTCGGCCGTCACGTCCATCACCTATCGACTGCACTGG
CCGCGGCCGGCCACGACGTGGTTGTCCTGTCTCGCCGCCCCTCGGGCACCGATCCCTGTACCCATCCGACCTCCGACGAG
ATCAGCGAAGGTGTGCGAGTGATCGCTGCCGCACAGGATCCACACGAGTTCACCTTCAGTAACGACATGATGGCTTGGAC
TCTGGCGATGGGCCATGCGATGATCCGCACCGGCCTGTCCCTAACCAGGCATAGCAGCGACCTCCCCTGGCGTCCCGATG
TCGTACATGCGCACGACTGGCTGGTGGCACATCCAGCCATAACGCTCGCCCAATTCTATGATGTTCCAATGGTTTCCACG
ATTCACGCGACAGAAGCAGGTCGGCATTCCGGCTGGGTATCTGGAGCACTCAGCCGTCAGGTACATGCCGTCGAGTCGTG
GCTGGTACGTGAATCCGATTCGTTGATCACATGTTCGGCATCGATGTGCAACGAAATCATCGAATTGTTTGGTCCCGGTC
TGGCCGAGATCACAGTGATCCGCAACGGTATTGACCCAGCACGCTGGCCGTTCGCAGCCCGCCGGGCGCGCACTGGACCG
GCCGAACTACTCTACGTCGGGCGACTAGAATATGAGAAGGGTGTGCACGACGTCATTGCCGCGCTACCCCGGATAAGGCG
CAGCTACCCTGGCACCACGTTGACCATCGCTGGAGAAGGAACCCAGCAGGATTGGCTCGTCGACCAAGCCCGTAAATACA
AAGTAATCAAAGCCACCCGGTTCGTCGGGCACCTCAACCACAACGAACTGCTGGCGGCGCTGCAGCGGGCCGACGCCGCA
GTGCTGCCGAGTCACTATGAGCCGTTCGGACTGGTCGCCCTAGAGGCCGCTGCAGCCGGCACTCCGCTGGTGACCTCAAA
CATAGGCGGCCTGGGCGAGGCGGTAATCAACGGGCAGACCGGGGTGTCGTGTCCGCCCCGCGATATCGCAGAACTGGCTG
CGATGGTGTGCACAGTACTTGAGGATCCCGACGCCGCGCAACAACGCGCGCTAGCTGCCCGAGAGCGTCTCACCTCCGAT
TTCGATTGGCAGACAGTAGCTCAGCAGACCGCGCAAGTGTACCTGGCGGCCAAGCGTCGCGAACGACAACCACAACCCAG
GTTGCCCATCGTCGAACACGCATTACCGGATCGCTAG

Upstream 100 bases:

>100_bases
GCGCAACTGGCGGACGGATGGAACCGCGCCGACGGTCTGTTCGGCGCACTGGACGCCCGAAGGCTGCCCCGGTGAAAATC
GGACTTCCCCGCGAGCGTGC

Downstream 100 bases:

>100_bases
TCTATACCGCGTCAGAGCCGAAACAGCCGCACAACATCAGGATGAAGTACTGCGCTCAGTTAGTATCCACCGGTGTTGGC
AACCTAAATAACTCGTCCCC

Product: putative transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MFIQRHAVVRGIFGTFAPAEGTTLMKILIVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRRPSGTDPCTHPTSDE
ISEGVRVIAAAQDPHEFTFSNDMMAWTLAMGHAMIRTGLSLTRHSSDLPWRPDVVHAHDWLVAHPAITLAQFYDVPMVST
IHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMCNEIIELFGPGLAEITVIRNGIDPARWPFAARRARTGP
AELLYVGRLEYEKGVHDVIAALPRIRRSYPGTTLTIAGEGTQQDWLVDQARKYKVIKATRFVGHLNHNELLAALQRADAA
VLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQTGVSCPPRDIAELAAMVCTVLEDPDAAQQRALAARERLTSD
FDWQTVAQQTAQVYLAAKRRERQPQPRLPIVEHALPDR

Sequences:

>Translated_438_residues
MFIQRHAVVRGIFGTFAPAEGTTLMKILIVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRRPSGTDPCTHPTSDE
ISEGVRVIAAAQDPHEFTFSNDMMAWTLAMGHAMIRTGLSLTRHSSDLPWRPDVVHAHDWLVAHPAITLAQFYDVPMVST
IHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMCNEIIELFGPGLAEITVIRNGIDPARWPFAARRARTGP
AELLYVGRLEYEKGVHDVIAALPRIRRSYPGTTLTIAGEGTQQDWLVDQARKYKVIKATRFVGHLNHNELLAALQRADAA
VLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQTGVSCPPRDIAELAAMVCTVLEDPDAAQQRALAARERLTSD
FDWQTVAQQTAQVYLAAKRRERQPQPRLPIVEHALPDR
>Mature_438_residues
MFIQRHAVVRGIFGTFAPAEGTTLMKILIVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRRPSGTDPCTHPTSDE
ISEGVRVIAAAQDPHEFTFSNDMMAWTLAMGHAMIRTGLSLTRHSSDLPWRPDVVHAHDWLVAHPAITLAQFYDVPMVST
IHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMCNEIIELFGPGLAEITVIRNGIDPARWPFAARRARTGP
AELLYVGRLEYEKGVHDVIAALPRIRRSYPGTTLTIAGEGTQQDWLVDQARKYKVIKATRFVGHLNHNELLAALQRADAA
VLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQTGVSCPPRDIAELAAMVCTVLEDPDAAQQRALAARERLTSD
FDWQTVAQQTAQVYLAAKRRERQPQPRLPIVEHALPDR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=400, Percent_Identity=22.5, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17532709, Length=402, Percent_Identity=20.3980099502488, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI9755344, Length=405, Percent_Identity=21.4814814814815, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654571, Length=339, Percent_Identity=25.3687315634218, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47735; Mature: 47735

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIQRHAVVRGIFGTFAPAEGTTLMKILIVSWEYPPVVIGGLGRHVHHLSTALAAAGHDV
CCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCE
VVLSRRPSGTDPCTHPTSDEISEGVRVIAAAQDPHEFTFSNDMMAWTLAMGHAMIRTGLS
EEEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCH
LTRHSSDLPWRPDVVHAHDWLVAHPAITLAQFYDVPMVSTIHATEAGRHSGWVSGALSRQ
HHCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHH
VHAVESWLVRESDSLITCSASMCNEIIELFGPGLAEITVIRNGIDPARWPFAARRARTGP
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCHHHHHCCCCC
AELLYVGRLEYEKGVHDVIAALPRIRRSYPGTTLTIAGEGTQQDWLVDQARKYKVIKATR
HHEEEEECCCHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
FVGHLNHNELLAALQRADAAVLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQT
HHHCCCHHHHHHHHHHCCHHCCCCCCCCCCEEEEHHHCCCCCEEECCCCCCCHHHCCCCC
GVSCPPRDIAELAAMVCTVLEDPDAAQQRALAARERLTSDFDWQTVAQQTAQVYLAAKRR
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERQPQPRLPIVEHALPDR
CCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MFIQRHAVVRGIFGTFAPAEGTTLMKILIVSWEYPPVVIGGLGRHVHHLSTALAAAGHDV
CCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCE
VVLSRRPSGTDPCTHPTSDEISEGVRVIAAAQDPHEFTFSNDMMAWTLAMGHAMIRTGLS
EEEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCCH
LTRHSSDLPWRPDVVHAHDWLVAHPAITLAQFYDVPMVSTIHATEAGRHSGWVSGALSRQ
HHCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHH
VHAVESWLVRESDSLITCSASMCNEIIELFGPGLAEITVIRNGIDPARWPFAARRARTGP
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCHHHHHCCCCC
AELLYVGRLEYEKGVHDVIAALPRIRRSYPGTTLTIAGEGTQQDWLVDQARKYKVIKATR
HHEEEEECCCHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
FVGHLNHNELLAALQRADAAVLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQT
HHHCCCHHHHHHHHHHCCHHCCCCCCCCCCEEEEHHHCCCCCEEECCCCCCCHHHCCCCC
GVSCPPRDIAELAAMVCTVLEDPDAAQQRALAARERLTSDFDWQTVAQQTAQVYLAAKRR
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ERQPQPRLPIVEHALPDR
CCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]