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The map label for this gene is ligA

Identifier: 221230388

GI number: 221230388

Start: 2055671

End: 2057755

Strand: Reverse

Name: ligA

Synonym: MLBr_01705

Alternate gene names: 221230388

Gene position: 2057755-2055671 (Counterclockwise)

Preceding gene: 221230389

Following gene: 221230386

Centisome position: 62.97

GC content: 60.62

Gene sequence:

>2085_bases
GTGAGTTCCCCCGAAGCTGACCCTATCGGACCCGAGGTGTTGCAGCAGTGGCGAAAGCTGACCGAAGAGGTGCGTGAACA
CCAGTTCCGTTACTATGTGCGTGACGCGCCGATCATCTCTGACGCGGAGTTTGATGCTCTGCTGGACAGGCTCACTGTGC
TGGAGGAACAGCATCCCGAGCTGTGCACGCCCGATTCACCTACCCAGCTGGTCGGCGGTGCTGGCTTCATGACTGATTTC
GTGGCGACCGAGCATCTCGAACGGATGTTCAGCCTCGACAACGCCTTCAGTGGGGATGAACTCAATGCCTGGGTCACTCG
CATCCGCGCAGAGATCGGTAATGACGCCCATTATTTGTGTGAGCTCAAGATCGACGGTGTCGCATTGTCGTTGGTCTACC
GGGATGGACGCCTGACCCGCGCCACGACTCGGGGCGATGGTCGCACCGGCGAAGACGTCACGCTGAACGCGCGGACCATC
GAAGATGTGCCGGAACGGCTGACCGTCAGCGATATTTACCCGCTGCCAGAGGTTCTTGAGGTCCGCGGCGAGGTGTTCTT
CCGAGTGGTGGACTTTCAAGCGCTTAACACGAGCCTCGTTGAGGAGGGCAAGAATCCGTTCGCTAACCCACGAAACAGCG
CGGCGGGCTCGCTGCGCCAGAAGGATCCGGCGGTCACGGCTCACCGTAAACTGCGAATGATCTGTCATGGACTGGGGCAC
ATCGAAGGCTTTCGTCCCGCTACCCAGCATCAGGCTTACTTAGCTTTACGGGACTGGGGGTTGCCGGTGTCCGAGCATAC
GACGCTGGTCAACGACATAGCTGGCGTCCAGGGATGCATCGCATACTGGGCCGAACATCGTCACGAGGTGGACCATGAAA
TCGACGGGATAGTGGTCAAAATCGACGAGGTTACGTTGCAGCGCAGGCTGGGTTCCACCTCGCGAGCTCCACGATGGGCC
ATCGCTTACAAGTACCTACCCGAGGAGGCGCAGACCAAGCTGCTCGACATCCGGGTGAACGTCGGCCGCACCGGTCGGGT
TACGCCATTCGCGTTCATGACGCCCGTGAAAGTTGCCGGGTCGACGGTCGGGCAGGCGACCTTGCACAACCCTTCTGAGG
TCAAACGCAAAGGTGTGCTGATCGGTGACACCGTGGTGATCCGCAAGGCCGGCGACGTGATTCCCGAAGTTTTGGGACCT
GTGGTTGACTTGCGCGACGGGTCCGAACGTGAATTTGTGATGCCCACAACGTGTCCCGAGTGCGGTACTACGTTAGCGCC
AGAAAAGGAAGGCGATGCCGACATCCGTTGCCCCAATGCCAGGAGCTGCCCGGGGCAACTGCGAGAGCGAGTGTTTCACG
TCGCTAGCCGCAGCGCTCTGGACATTCAGGGGTTGGGTTACGAGGCCGGTGTGGCGTTGCTGGCTGCGCAAGTGATCACT
AGCGAGGGCGACCTGTTCACCCTCACCGAGAAGGCCTTGTTGCGCACCGAGCTTTTCCGCAACAAGGCAGGGGAGTTGTC
GGCGAATGGCAAACGGTTGCTAGTCAACGTCGACAAAGCCAAAACTGCACCGCTGTGGCGAGTGCTGGTGGCGTTGTCGA
TCCGGCATGTCGGGCCGACGGCTGCGCGAGCCTTGGCCACCGAGTTCGGCAGCGTTGACGCAATCTTGGCCGCGTCCCCT
GAACAACTGGCCGCTGTCGAGGGTGTAGGGACCACCATCGCGGCCGCGGTTACCGAGTGGTTCACCGTCGACTGGCATCG
CGTGATTGTCAATAAGTGGCGGGCTGCCGGGGTGCGGATGGTCGATGAGCGTGACACCAGCGTGCTGCCCACATGCGAAG
GGTTGACCATCGTGGTCACTGGTTCTTTAGCAGGGTTTTCCCGTGACGATGCCAAGGAGGCGATCGTGGCGCGCGGAGGC
AAGGTCGCTGGTTCGGTATCGAAGAAGATCGCCTACGTGGTCGTAGGCGACTTGCCGGGATACAAATATGACAAGGCGGT
CGAGCTCGGGGTGCCGATCCTCAACGAGGACGGGTTTCGCCTGTTGCTTGAGCAGGGACCTCCGGTGCAGCAAGTCGTTG
ACTAG

Upstream 100 bases:

>100_bases
AAGACCGCCGAAGCGTTCGCCCAAGACCCGGACGCCATCTAGTTGCGTCGGCGCACCAGCGGCCCAGTCGCGTGTCGGAT
TGCTCAGTTAGCCTGCGAGG

Downstream 100 bases:

>100_bases
CGTTAGCGCAGCATGGTGGCCACGATTCCGGCTAGGTACCCCAGTCGAGCCACCTCTGATGGCCTAAACTCCGGGCCCGG
ACGGCCCAGCACCACTGCGG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MSSPEADPIGPEVLQQWRKLTEEVREHQFRYYVRDAPIISDAEFDALLDRLTVLEEQHPELCTPDSPTQLVGGAGFMTDF
VATEHLERMFSLDNAFSGDELNAWVTRIRAEIGNDAHYLCELKIDGVALSLVYRDGRLTRATTRGDGRTGEDVTLNARTI
EDVPERLTVSDIYPLPEVLEVRGEVFFRVVDFQALNTSLVEEGKNPFANPRNSAAGSLRQKDPAVTAHRKLRMICHGLGH
IEGFRPATQHQAYLALRDWGLPVSEHTTLVNDIAGVQGCIAYWAEHRHEVDHEIDGIVVKIDEVTLQRRLGSTSRAPRWA
IAYKYLPEEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAGSTVGQATLHNPSEVKRKGVLIGDTVVIRKAGDVIPEVLGP
VVDLRDGSEREFVMPTTCPECGTTLAPEKEGDADIRCPNARSCPGQLRERVFHVASRSALDIQGLGYEAGVALLAAQVIT
SEGDLFTLTEKALLRTELFRNKAGELSANGKRLLVNVDKAKTAPLWRVLVALSIRHVGPTAARALATEFGSVDAILAASP
EQLAAVEGVGTTIAAAVTEWFTVDWHRVIVNKWRAAGVRMVDERDTSVLPTCEGLTIVVTGSLAGFSRDDAKEAIVARGG
KVAGSVSKKIAYVVVGDLPGYKYDKAVELGVPILNEDGFRLLLEQGPPVQQVVD

Sequences:

>Translated_694_residues
MSSPEADPIGPEVLQQWRKLTEEVREHQFRYYVRDAPIISDAEFDALLDRLTVLEEQHPELCTPDSPTQLVGGAGFMTDF
VATEHLERMFSLDNAFSGDELNAWVTRIRAEIGNDAHYLCELKIDGVALSLVYRDGRLTRATTRGDGRTGEDVTLNARTI
EDVPERLTVSDIYPLPEVLEVRGEVFFRVVDFQALNTSLVEEGKNPFANPRNSAAGSLRQKDPAVTAHRKLRMICHGLGH
IEGFRPATQHQAYLALRDWGLPVSEHTTLVNDIAGVQGCIAYWAEHRHEVDHEIDGIVVKIDEVTLQRRLGSTSRAPRWA
IAYKYLPEEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAGSTVGQATLHNPSEVKRKGVLIGDTVVIRKAGDVIPEVLGP
VVDLRDGSEREFVMPTTCPECGTTLAPEKEGDADIRCPNARSCPGQLRERVFHVASRSALDIQGLGYEAGVALLAAQVIT
SEGDLFTLTEKALLRTELFRNKAGELSANGKRLLVNVDKAKTAPLWRVLVALSIRHVGPTAARALATEFGSVDAILAASP
EQLAAVEGVGTTIAAAVTEWFTVDWHRVIVNKWRAAGVRMVDERDTSVLPTCEGLTIVVTGSLAGFSRDDAKEAIVARGG
KVAGSVSKKIAYVVVGDLPGYKYDKAVELGVPILNEDGFRLLLEQGPPVQQVVD
>Mature_693_residues
SSPEADPIGPEVLQQWRKLTEEVREHQFRYYVRDAPIISDAEFDALLDRLTVLEEQHPELCTPDSPTQLVGGAGFMTDFV
ATEHLERMFSLDNAFSGDELNAWVTRIRAEIGNDAHYLCELKIDGVALSLVYRDGRLTRATTRGDGRTGEDVTLNARTIE
DVPERLTVSDIYPLPEVLEVRGEVFFRVVDFQALNTSLVEEGKNPFANPRNSAAGSLRQKDPAVTAHRKLRMICHGLGHI
EGFRPATQHQAYLALRDWGLPVSEHTTLVNDIAGVQGCIAYWAEHRHEVDHEIDGIVVKIDEVTLQRRLGSTSRAPRWAI
AYKYLPEEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAGSTVGQATLHNPSEVKRKGVLIGDTVVIRKAGDVIPEVLGPV
VDLRDGSEREFVMPTTCPECGTTLAPEKEGDADIRCPNARSCPGQLRERVFHVASRSALDIQGLGYEAGVALLAAQVITS
EGDLFTLTEKALLRTELFRNKAGELSANGKRLLVNVDKAKTAPLWRVLVALSIRHVGPTAARALATEFGSVDAILAASPE
QLAAVEGVGTTIAAAVTEWFTVDWHRVIVNKWRAAGVRMVDERDTSVLPTCEGLTIVVTGSLAGFSRDDAKEAIVARGGK
VAGSVSKKIAYVVVGDLPGYKYDKAVELGVPILNEDGFRLLLEQGPPVQQVVD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=41.9734904270987, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MYCLB (B8ZS27)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503804.1
- ProteinModelPortal:   B8ZS27
- EnsemblBacteria:   EBMYCT00000085279
- GeneID:   7326798
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016652
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75913; Mature: 75782

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 184-184 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPEADPIGPEVLQQWRKLTEEVREHQFRYYVRDAPIISDAEFDALLDRLTVLEEQHPE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
LCTPDSPTQLVGGAGFMTDFVATEHLERMFSLDNAFSGDELNAWVTRIRAEIGNDAHYLC
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEE
ELKIDGVALSLVYRDGRLTRATTRGDGRTGEDVTLNARTIEDVPERLTVSDIYPLPEVLE
EEEECCEEEEEEEECCCEEEEEECCCCCCCCCEEEECHHHHHHHHHCCHHHCCCCHHHHH
VRGEVFFRVVDFQALNTSLVEEGKNPFANPRNSAAGSLRQKDPAVTAHRKLRMICHGLGH
HHHHHEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
IEGFRPATQHQAYLALRDWGLPVSEHTTLVNDIAGVQGCIAYWAEHRHEVDHEIDGIVVK
CCCCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
IDEVTLQRRLGSTSRAPRWAIAYKYLPEEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAG
EHHHHHHHHCCCCCCCCCEEEEEEECCCHHHCEEEEEEEECCCCCCCCCCCEECCHHHCC
STVGQATLHNPSEVKRKGVLIGDTVVIRKAGDVIPEVLGPVVDLRDGSEREFVMPTTCPE
CCCCCCCCCCHHHHHHCCEEECCEEEEECCHHHHHHHHCCEEECCCCCCCCEECCCCCCC
CGTTLAPEKEGDADIRCPNARSCPGQLRERVFHVASRSALDIQGLGYEAGVALLAAQVIT
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHC
SEGDLFTLTEKALLRTELFRNKAGELSANGKRLLVNVDKAKTAPLWRVLVALSIRHVGPT
CCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHH
AARALATEFGSVDAILAASPEQLAAVEGVGTTIAAAVTEWFTVDWHRVIVNKWRAAGVRM
HHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VDERDTSVLPTCEGLTIVVTGSLAGFSRDDAKEAIVARGGKVAGSVSKKIAYVVVGDLPG
ECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC
YKYDKAVELGVPILNEDGFRLLLEQGPPVQQVVD
CCCCCEEEECCEEECCCCCEEEECCCCCHHHHCC
>Mature Secondary Structure 
SSPEADPIGPEVLQQWRKLTEEVREHQFRYYVRDAPIISDAEFDALLDRLTVLEEQHPE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
LCTPDSPTQLVGGAGFMTDFVATEHLERMFSLDNAFSGDELNAWVTRIRAEIGNDAHYLC
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEE
ELKIDGVALSLVYRDGRLTRATTRGDGRTGEDVTLNARTIEDVPERLTVSDIYPLPEVLE
EEEECCEEEEEEEECCCEEEEEECCCCCCCCCEEEECHHHHHHHHHCCHHHCCCCHHHHH
VRGEVFFRVVDFQALNTSLVEEGKNPFANPRNSAAGSLRQKDPAVTAHRKLRMICHGLGH
HHHHHEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
IEGFRPATQHQAYLALRDWGLPVSEHTTLVNDIAGVQGCIAYWAEHRHEVDHEIDGIVVK
CCCCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
IDEVTLQRRLGSTSRAPRWAIAYKYLPEEAQTKLLDIRVNVGRTGRVTPFAFMTPVKVAG
EHHHHHHHHCCCCCCCCCEEEEEEECCCHHHCEEEEEEEECCCCCCCCCCCEECCHHHCC
STVGQATLHNPSEVKRKGVLIGDTVVIRKAGDVIPEVLGPVVDLRDGSEREFVMPTTCPE
CCCCCCCCCCHHHHHHCCEEECCEEEEECCHHHHHHHHCCEEECCCCCCCCEECCCCCCC
CGTTLAPEKEGDADIRCPNARSCPGQLRERVFHVASRSALDIQGLGYEAGVALLAAQVIT
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHC
SEGDLFTLTEKALLRTELFRNKAGELSANGKRLLVNVDKAKTAPLWRVLVALSIRHVGPT
CCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHH
AARALATEFGSVDAILAASPEQLAAVEGVGTTIAAAVTEWFTVDWHRVIVNKWRAAGVRM
HHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VDERDTSVLPTCEGLTIVVTGSLAGFSRDDAKEAIVARGGKVAGSVSKKIAYVVVGDLPG
ECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCC
YKYDKAVELGVPILNEDGFRLLLEQGPPVQQVVD
CCCCCEEEECCEEECCCCCEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA