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The map label for this gene is rnhB

Identifier: 221230324

GI number: 221230324

Start: 1935364

End: 1936086

Strand: Reverse

Name: rnhB

Synonym: MLBr_01611

Alternate gene names: 221230324

Gene position: 1936086-1935364 (Counterclockwise)

Preceding gene: 221230325

Following gene: 221230323

Centisome position: 59.24

GC content: 63.49

Gene sequence:

>723_bases
ATGGCCACCACCTGGCCGCCGTGCAGGATAATCCGTAAGTCTGGGGGTTTGCGGGGGATGTGGACTCTAGAGTACGAACT
GCAGCGCAGCGGGTTGGGGCCGGTGGCAGGGGTGGACGAGGTGGGTCGCGGCGCCTGCGCGGGTCCGCTGGTGGTGGCAG
CTTGTGTGCTCGGGCCGGGGCGACTGGAGGAAAGTCTTGATGATTCCAAGAAGCTTAGCGCGAAGGGGCGGGAGATGTTG
TTTCCTTTGATCTGCCGGTACGCGCTGGCTTACCACGTGGTGTTCATCCCGTCGGTTGAGGTCGACCGGCATGGTGTGCA
GGTAGCCAATATTGAAGGCATGCGGCGTGCGGTGGCCGGTCTGTCGGTGCGGCCCGGCTATGTATTAAGCGACGGTTTTC
GCGTACCCGGCCTGTCGGTGCCGTCGCTGCCGGTAGTTGGTGGAGACGCGGTGGTTGCCTGCATTGCCGCGGCCAGTGTG
CTGGCCAAAGTCAGTCGTGACCGGTTGATGGTCGCAATGGACGCTGACTATCCTGGCTACGGCTTCGCTGCACACAAGGG
GTACTGCACGCGCGCGCATAGCTTGGCGCTCACCCAGCTGGGGCCTTGCCCAGAACACCGCTATTCGTTCATCAACGTTC
GGCGCATAGTGACCAGGTCTAATACCCGGGCGGTGGCTGGATTCACACCGGCTCCTCCGGCCGAGCACGGCGAATGCCGG
TGA

Upstream 100 bases:

>100_bases
TGCCGGTGTCCAATGTCATCGGTAAGGCCAGGGTCGTCGTGTGGCCGCCGTCACGATGGGGTGGTGTGGGTTCGGTGAAT
TCCCAGCAGGGGCAGTAGCC

Downstream 100 bases:

>100_bases
GAAAAGATGGGACCGGAATTCCCACTTGGTCACGGTAGTGTGTGGCTCAAAGAGTCCAAGGGGCCCAATAGGGACGTCGG
GAAAAGGGCCAGAGAGAAGG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MATTWPPCRIIRKSGGLRGMWTLEYELQRSGLGPVAGVDEVGRGACAGPLVVAACVLGPGRLEESLDDSKKLSAKGREML
FPLICRYALAYHVVFIPSVEVDRHGVQVANIEGMRRAVAGLSVRPGYVLSDGFRVPGLSVPSLPVVGGDAVVACIAAASV
LAKVSRDRLMVAMDADYPGYGFAAHKGYCTRAHSLALTQLGPCPEHRYSFINVRRIVTRSNTRAVAGFTPAPPAEHGECR

Sequences:

>Translated_240_residues
MATTWPPCRIIRKSGGLRGMWTLEYELQRSGLGPVAGVDEVGRGACAGPLVVAACVLGPGRLEESLDDSKKLSAKGREML
FPLICRYALAYHVVFIPSVEVDRHGVQVANIEGMRRAVAGLSVRPGYVLSDGFRVPGLSVPSLPVVGGDAVVACIAAASV
LAKVSRDRLMVAMDADYPGYGFAAHKGYCTRAHSLALTQLGPCPEHRYSFINVRRIVTRSNTRAVAGFTPAPPAEHGECR
>Mature_239_residues
ATTWPPCRIIRKSGGLRGMWTLEYELQRSGLGPVAGVDEVGRGACAGPLVVAACVLGPGRLEESLDDSKKLSAKGREMLF
PLICRYALAYHVVFIPSVEVDRHGVQVANIEGMRRAVAGLSVRPGYVLSDGFRVPGLSVPSLPVVGGDAVVACIAAASVL
AKVSRDRLMVAMDADYPGYGFAAHKGYCTRAHSLALTQLGPCPEHRYSFINVRRIVTRSNTRAVAGFTPAPPAEHGECR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=181, Percent_Identity=48.0662983425414, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_MYCLB (B8ZRW3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503740.1
- ProteinModelPortal:   B8ZRW3
- SMR:   B8ZRW3
- EnsemblBacteria:   EBMYCT00000085547
- GeneID:   7325773
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016477
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25600; Mature: 25469

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTWPPCRIIRKSGGLRGMWTLEYELQRSGLGPVAGVDEVGRGACAGPLVVAACVLGPG
CCCCCCHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCH
RLEESLDDSKKLSAKGREMLFPLICRYALAYHVVFIPSVEVDRHGVQVANIEGMRRAVAG
HHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCEECCCCEEEECHHHHHHHHHC
LSVRPGYVLSDGFRVPGLSVPSLPVVGGDAVVACIAAASVLAKVSRDRLMVAMDADYPGY
CCCCCCEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GFAAHKGYCTRAHSLALTQLGPCPEHRYSFINVRRIVTRSNTRAVAGFTPAPPAEHGECR
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
ATTWPPCRIIRKSGGLRGMWTLEYELQRSGLGPVAGVDEVGRGACAGPLVVAACVLGPG
CCCCCHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCH
RLEESLDDSKKLSAKGREMLFPLICRYALAYHVVFIPSVEVDRHGVQVANIEGMRRAVAG
HHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCEECCCCEEEECHHHHHHHHHC
LSVRPGYVLSDGFRVPGLSVPSLPVVGGDAVVACIAAASVLAKVSRDRLMVAMDADYPGY
CCCCCCEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
GFAAHKGYCTRAHSLALTQLGPCPEHRYSFINVRRIVTRSNTRAVAGFTPAPPAEHGECR
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA