Click here to switch to the map view.

The map label for this gene is truB

Identifier: 221230290

GI number: 221230290

Start: 1870924

End: 1871886

Strand: Reverse

Name: truB

Synonym: MLBr_01546

Alternate gene names: 221230290

Gene position: 1871886-1870924 (Counterclockwise)

Preceding gene: 221230291

Following gene: 221230289

Centisome position: 57.28

GC content: 62.31

Gene sequence:

>963_bases
ATGAGCGAAAGCTCCACAGGTGCAGGACTCGGTCCGGGAATAGTGGTCATTGACAAGCCGTCCGGGATGACCAGTCACGA
TGTGGTGGCGCGCTGTCGCCGAATATTTTGCACTCGCAGGGTGGGGCACGCGGGAACGCTGGATCCGATGGCCACCGGTG
TGCTGGTGCTCGGGGTCGATCGAGCCACCAAGATCTTGGGTCTGCTGGCCGGGGCCTCGAAAGAGTACGTCGCGACCATC
CGCCTAGGTCAGACCACATCTACTGAAGACGCCGAAGGTGAACTGCTGCAGTGTGTCTCGGCTCGGCACGTGACAGACGA
GGCGATCGCCACCGCGATCGGTAGGCTGCGCGGTGATATCAAGCAGGTGCCGTCGGCGGTCAGTGCTATCAAGGTCGACG
GCCGTCGTGCTTATCGACTGGTCCGCGAGGGTCATGTTGTTGAGCTGCAAGCCCGGCCGGTACGCATTGACCGGTTTGAG
GTGCTGGCTGTTCGACCCGGGCCCGAAGTCGGATTAGCCGATGTCATCGATCTTGATGTGGAAGTCGAATGCTCATCGGG
AACCTATATTCGTGCGCTGGCTCGCGACCTCGGCGATGCGTTCGGGGTAGGTGGGCATCTGACGTCGTTGCGGCGCACCC
GGGTCGGCCGTTTCGAGCTGGACCAGGCGTGGTCACTCGAGGATCTGGCGGAGCTACCGCGGTTGAGTCGGACGCTAGAC
GAGACCTGTTTGCTGATGTTTCCTCGCCGTGACCTTACGGTAAGCGAGGTCGAGGCAACCAGCAACGGGCGACCAATTTC
GTCGGCCGGTATCGACGGCATCTATGCCGCCAGCGACGCCGACGGCCGAGTCATTGCATTGCTGCGCGACGAGGGCCCGC
GGACCAAGTCGGTGGTGGTGCTGCACCCGGTCTTTGTGCGAGTCAGCATCGGACGGGGCATTGTTGGGGTGATTGAGGCC
TAA

Upstream 100 bases:

>100_bases
GGATCCTGGTCGACGGATCGGCATTGTCGGGTCCACCGTTAACGGTGCTGACTGGTCGGTGGTCAGTCGATCGCGGGTTG
GTGCTGACCGCAATCGTGCT

Downstream 100 bases:

>100_bases
GGGTGGTGTTGTGGTGGGCTGGTGGGGTGTGGGTGGTTGATCTGCGCTAGAAGGTTGCCGTATGTGCCGCCGACGGCCGG
ATCATCGATGCACTGTTCAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRIFCTRRVGHAGTLDPMATGVLVLGVDRATKILGLLAGASKEYVATI
RLGQTTSTEDAEGELLQCVSARHVTDEAIATAIGRLRGDIKQVPSAVSAIKVDGRRAYRLVREGHVVELQARPVRIDRFE
VLAVRPGPEVGLADVIDLDVEVECSSGTYIRALARDLGDAFGVGGHLTSLRRTRVGRFELDQAWSLEDLAELPRLSRTLD
ETCLLMFPRRDLTVSEVEATSNGRPISSAGIDGIYAASDADGRVIALLRDEGPRTKSVVVLHPVFVRVSIGRGIVGVIEA

Sequences:

>Translated_320_residues
MSESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRIFCTRRVGHAGTLDPMATGVLVLGVDRATKILGLLAGASKEYVATI
RLGQTTSTEDAEGELLQCVSARHVTDEAIATAIGRLRGDIKQVPSAVSAIKVDGRRAYRLVREGHVVELQARPVRIDRFE
VLAVRPGPEVGLADVIDLDVEVECSSGTYIRALARDLGDAFGVGGHLTSLRRTRVGRFELDQAWSLEDLAELPRLSRTLD
ETCLLMFPRRDLTVSEVEATSNGRPISSAGIDGIYAASDADGRVIALLRDEGPRTKSVVVLHPVFVRVSIGRGIVGVIEA
>Mature_319_residues
SESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRIFCTRRVGHAGTLDPMATGVLVLGVDRATKILGLLAGASKEYVATIR
LGQTTSTEDAEGELLQCVSARHVTDEAIATAIGRLRGDIKQVPSAVSAIKVDGRRAYRLVREGHVVELQARPVRIDRFEV
LAVRPGPEVGLADVIDLDVEVECSSGTYIRALARDLGDAFGVGGHLTSLRRTRVGRFELDQAWSLEDLAELPRLSRTLDE
TCLLMFPRRDLTVSEVEATSNGRPISSAGIDGIYAASDADGRVIALLRDEGPRTKSVVVLHPVFVRVSIGRGIVGVIEA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=272, Percent_Identity=31.9852941176471, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4503337, Length=247, Percent_Identity=31.1740890688259, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI215599015, Length=247, Percent_Identity=31.1740890688259, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI7661556, Length=216, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367200, Length=315, Percent_Identity=34.9206349206349, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553978, Length=207, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=237, Percent_Identity=32.4894514767932, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=170, Percent_Identity=30, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364189, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281364187, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281364185, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281364183, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI62471759, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17975520, Length=282, Percent_Identity=29.4326241134752, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_MYCLE (Q9Z5I4)

Other databases:

- EMBL:   AL035472
- EMBL:   AL583922
- PIR:   D87102
- RefSeq:   NP_302076.1
- ProteinModelPortal:   Q9Z5I4
- SMR:   Q9Z5I4
- EnsemblBacteria:   EBMYCT00000028927
- GeneID:   909603
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1546
- NMPDR:   fig|272631.1.peg.948
- Leproma:   ML1546
- GeneTree:   EBGT00050000016018
- HOGENOM:   HBG397258
- OMA:   EYLGTIR
- ProtClustDB:   PRK03287
- BioCyc:   MLEP272631:ML1546-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34181; Mature: 34049

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRIFCTRRVGHAGTLDPMATGVLVLGVD
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEECC
RATKILGLLAGASKEYVATIRLGQTTSTEDAEGELLQCVSARHVTDEAIATAIGRLRGDI
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KQVPSAVSAIKVDGRRAYRLVREGHVVELQARPVRIDRFEVLAVRPGPEVGLADVIDLDV
HHHHHHHHEEEECCHHHHHHHHCCCEEEEECCCEEECEEEEEEECCCCCCCCCEEEEEEE
EVECSSGTYIRALARDLGDAFGVGGHLTSLRRTRVGRFELDQAWSLEDLAELPRLSRTLD
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHC
ETCLLMFPRRDLTVSEVEATSNGRPISSAGIDGIYAASDADGRVIALLRDEGPRTKSVVV
CEEEEEECCCCCCHHHEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEE
LHPVFVRVSIGRGIVGVIEA
EEEEEEEEECCCCEEEEECC
>Mature Secondary Structure 
SESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRIFCTRRVGHAGTLDPMATGVLVLGVD
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEECC
RATKILGLLAGASKEYVATIRLGQTTSTEDAEGELLQCVSARHVTDEAIATAIGRLRGDI
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KQVPSAVSAIKVDGRRAYRLVREGHVVELQARPVRIDRFEVLAVRPGPEVGLADVIDLDV
HHHHHHHHEEEECCHHHHHHHHCCCEEEEECCCEEECEEEEEEECCCCCCCCCEEEEEEE
EVECSSGTYIRALARDLGDAFGVGGHLTSLRRTRVGRFELDQAWSLEDLAELPRLSRTLD
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHC
ETCLLMFPRRDLTVSEVEATSNGRPISSAGIDGIYAASDADGRVIALLRDEGPRTKSVVV
CEEEEEECCCCCCHHHEEECCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEE
LHPVFVRVSIGRGIVGVIEA
EEEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002