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The map label for this gene is folC [H]

Identifier: 221230250

GI number: 221230250

Start: 1768915

End: 1770372

Strand: Reverse

Name: folC [H]

Synonym: MLBr_01471

Alternate gene names: 221230250

Gene position: 1770372-1768915 (Counterclockwise)

Preceding gene: 221230251

Following gene: 221230249

Centisome position: 54.17

GC content: 61.52

Gene sequence:

>1458_bases
ATGACCGCAGAGCTGCCTGACTGGGGATCCGGACGTCTTGAAACCAATGTCGCTGCTCTCACACCGGACAAGGTCGCCTC
CCTGTTGCAGGTTGAACACCTGCTGGACCAGCGCTGGCCAGAGACCTGTATCGAACCGAGTTTGACTCGGATCAGTGCGT
TAATGGATGTTCTCGGCTCACCGCAACTCAGTTATCCGTCGATACACGTCGCTGGCACCAACGGGAAGACCTCGGTGACG
AGGATGGTTGACGCGCTGCTAACCGCGCTGCACCAGCGCACTGGCCGCTTTACCAGCCCTCACCTGCAGTCGGTAGTCGA
ACGCATCGCGATTGACTCAAAACCGATCAGCCCGGCGCAATATGTAGCAACTTACCGTGAGGTTGAACCGTTTGTGCAGA
TGATCGATGCCCAGTCGCAGTCCTCTGGGGGGCCGGCGCTGAGCAAGTTCGAGGTAGTCACCGCGATGGCGTTCGCCGCG
TTCGCGAATGCGCCCGTCGACATCGCGGTGGTTGAGGTGGGCCTCGGTGGGCGATGGGATGCGACCAACGTGATTAATGC
ATCGGTCGCCGTGATCACCCCGGTCAGCATCGACCACGTCGAGTATCTTGGCAACGACGTTGCAGCGATCGCCCGCGAGA
AGGCGGGCATTATCACGAAAGCTCCCGAAGGCGCGCCAGACACCGTCGTGGTAATCGGTCGTCAGGTACCTGCGGCGATG
GGGGTGCTGTTGGCTCAATCGGCACATGCCGGCGCTCGAGTCGTCCGTCAGGATTCGGAGTTCGCGGTCTTGGACCGTCA
GGTCGCAATCGGTGGTCAGGTGTTGCAGCTGCAGGGGCTTGGTGGAGTTTATAATGACATTTACCTGCCGTTACATGGCG
AACACCAGGCGCACAACGCGGTCTTGGTGCTTGCTGCGGTCGAGGCGTTTTATGGTGCCGGGGTGCAGCGCCAGCTCGAT
GTCGAGGCGGTGCGGTCCGGTTTCGCCGCTGTCATCAGCCCCGGCAGGTTGGAACGCATGCGCAGTGCCCCGACGGTGTT
CATCGACGCCGCTCACAATCCCGCCGGAGCGAATGCGTTGGCGCGCACGCTGTCTGACGAATTTAATTTCCGAACCCTGG
TCGGGGTGCTCAGCGTGCTGGCAAACAAGGACGTTGATGGCATCCTGGCCGCACTGGAGCTGGTGCTGGATTATATCGTT
GTGACTGACAACGGCTCGCCGCGGGCGTTGGATGTTGAGTCGCTGGCGTTGGTGGCGCGGGAGCGGTTCGGCCCCGACCG
GGTAATCAGCGCGGCGAACCTGCGTGACGCTATCGACGCAGCGACTGCACTGGTCGACGACGCCGCTGCCAATGAGGAAA
TCGAAGGGCTCGGAGTCTCCGGCCGTGGGATCGTCATCACCGGCTCGGTGATCACCGCCGGAATCGCACGGACTTTGTTT
GGCCGTGACCCACAATGA

Upstream 100 bases:

>100_bases
TAGCTAAAGCCCCGGAAGCAGTCGTTGTCAAAATCCGCGATCGTCAGCGGATGGCTAAGGAAGAAACCGACCGCATCATC
GCTAGGCTGGCTGGGCTGCA

Downstream 100 bases:

>100_bases
CGGACAGCCCGGGGGAGCACGGTCCGCAAAAGCCTCTGCCATCTGCCGACCCCTGGAAGAGCTTTGCCGGGGTGATGGCT
GCTATACTCTTTCTTGAGGC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MTAELPDWGSGRLETNVAALTPDKVASLLQVEHLLDQRWPETCIEPSLTRISALMDVLGSPQLSYPSIHVAGTNGKTSVT
RMVDALLTALHQRTGRFTSPHLQSVVERIAIDSKPISPAQYVATYREVEPFVQMIDAQSQSSGGPALSKFEVVTAMAFAA
FANAPVDIAVVEVGLGGRWDATNVINASVAVITPVSIDHVEYLGNDVAAIAREKAGIITKAPEGAPDTVVVIGRQVPAAM
GVLLAQSAHAGARVVRQDSEFAVLDRQVAIGGQVLQLQGLGGVYNDIYLPLHGEHQAHNAVLVLAAVEAFYGAGVQRQLD
VEAVRSGFAAVISPGRLERMRSAPTVFIDAAHNPAGANALARTLSDEFNFRTLVGVLSVLANKDVDGILAALELVLDYIV
VTDNGSPRALDVESLALVARERFGPDRVISAANLRDAIDAATALVDDAAANEEIEGLGVSGRGIVITGSVITAGIARTLF
GRDPQ

Sequences:

>Translated_485_residues
MTAELPDWGSGRLETNVAALTPDKVASLLQVEHLLDQRWPETCIEPSLTRISALMDVLGSPQLSYPSIHVAGTNGKTSVT
RMVDALLTALHQRTGRFTSPHLQSVVERIAIDSKPISPAQYVATYREVEPFVQMIDAQSQSSGGPALSKFEVVTAMAFAA
FANAPVDIAVVEVGLGGRWDATNVINASVAVITPVSIDHVEYLGNDVAAIAREKAGIITKAPEGAPDTVVVIGRQVPAAM
GVLLAQSAHAGARVVRQDSEFAVLDRQVAIGGQVLQLQGLGGVYNDIYLPLHGEHQAHNAVLVLAAVEAFYGAGVQRQLD
VEAVRSGFAAVISPGRLERMRSAPTVFIDAAHNPAGANALARTLSDEFNFRTLVGVLSVLANKDVDGILAALELVLDYIV
VTDNGSPRALDVESLALVARERFGPDRVISAANLRDAIDAATALVDDAAANEEIEGLGVSGRGIVITGSVITAGIARTLF
GRDPQ
>Mature_484_residues
TAELPDWGSGRLETNVAALTPDKVASLLQVEHLLDQRWPETCIEPSLTRISALMDVLGSPQLSYPSIHVAGTNGKTSVTR
MVDALLTALHQRTGRFTSPHLQSVVERIAIDSKPISPAQYVATYREVEPFVQMIDAQSQSSGGPALSKFEVVTAMAFAAF
ANAPVDIAVVEVGLGGRWDATNVINASVAVITPVSIDHVEYLGNDVAAIAREKAGIITKAPEGAPDTVVVIGRQVPAAMG
VLLAQSAHAGARVVRQDSEFAVLDRQVAIGGQVLQLQGLGGVYNDIYLPLHGEHQAHNAVLVLAAVEAFYGAGVQRQLDV
EAVRSGFAAVISPGRLERMRSAPTVFIDAAHNPAGANALARTLSDEFNFRTLVGVLSVLANKDVDGILAALELVLDYIVV
TDNGSPRALDVESLALVARERFGPDRVISAANLRDAIDAATALVDDAAANEEIEGLGVSGRGIVITGSVITAGIARTLFG
RDPQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=376, Percent_Identity=29.5212765957447, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI66932990, Length=376, Percent_Identity=29.5212765957447, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788654, Length=376, Percent_Identity=30.8510638297872, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=155, Percent_Identity=36.7741935483871, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17553148, Length=155, Percent_Identity=36.7741935483871, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17553150, Length=155, Percent_Identity=36.7741935483871, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323760, Length=373, Percent_Identity=32.9758713136729, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=455, Percent_Identity=25.2747252747253, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322718, Length=193, Percent_Identity=26.4248704663212, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641571, Length=158, Percent_Identity=38.6075949367089, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24581568, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 51054; Mature: 50922

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELPDWGSGRLETNVAALTPDKVASLLQVEHLLDQRWPETCIEPSLTRISALMDVLGS
CCCCCCCCCCCCEECCEEEECHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCC
PQLSYPSIHVAGTNGKTSVTRMVDALLTALHQRTGRFTSPHLQSVVERIAIDSKPISPAQ
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHH
YVATYREVEPFVQMIDAQSQSSGGPALSKFEVVTAMAFAAFANAPVDIAVVEVGLGGRWD
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
ATNVINASVAVITPVSIDHVEYLGNDVAAIAREKAGIITKAPEGAPDTVVVIGRQVPAAM
CCHHCCCEEEEEECCCHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCEEEEECCCCHHHH
GVLLAQSAHAGARVVRQDSEFAVLDRQVAIGGQVLQLQGLGGVYNDIYLPLHGEHQAHNA
HHHHHHCCCCCHHHHCCCCCEEEEHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
VLVLAAVEAFYGAGVQRQLDVEAVRSGFAAVISPGRLERMRSAPTVFIDAAHNPAGANAL
EEHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHHHHCCCEEEEECCCCCCCHHHH
ARTLSDEFNFRTLVGVLSVLANKDVDGILAALELVLDYIVVTDNGSPRALDVESLALVAR
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCCCEECHHHHHHHHH
ERFGPDRVISAANLRDAIDAATALVDDAAANEEIEGLGVSGRGIVITGSVITAGIARTLF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHH
GRDPQ
CCCCC
>Mature Secondary Structure 
TAELPDWGSGRLETNVAALTPDKVASLLQVEHLLDQRWPETCIEPSLTRISALMDVLGS
CCCCCCCCCCCEECCEEEECHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCC
PQLSYPSIHVAGTNGKTSVTRMVDALLTALHQRTGRFTSPHLQSVVERIAIDSKPISPAQ
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHH
YVATYREVEPFVQMIDAQSQSSGGPALSKFEVVTAMAFAAFANAPVDIAVVEVGLGGRWD
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
ATNVINASVAVITPVSIDHVEYLGNDVAAIAREKAGIITKAPEGAPDTVVVIGRQVPAAM
CCHHCCCEEEEEECCCHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCEEEEECCCCHHHH
GVLLAQSAHAGARVVRQDSEFAVLDRQVAIGGQVLQLQGLGGVYNDIYLPLHGEHQAHNA
HHHHHHCCCCCHHHHCCCCCEEEEHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
VLVLAAVEAFYGAGVQRQLDVEAVRSGFAAVISPGRLERMRSAPTVFIDAAHNPAGANAL
EEHHHHHHHHHCCCCCCCCCHHHHHCCHHHHCCCHHHHHHHCCCEEEEECCCCCCCHHHH
ARTLSDEFNFRTLVGVLSVLANKDVDGILAALELVLDYIVVTDNGSPRALDVESLALVAR
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCCCCEECHHHHHHHHH
ERFGPDRVISAANLRDAIDAATALVDDAAANEEIEGLGVSGRGIVITGSVITAGIARTLF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHH
GRDPQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]