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The map label for this gene is rpoT [H]

Identifier: 221229969

GI number: 221229969

Start: 1189225

End: 1190949

Strand: Reverse

Name: rpoT [H]

Synonym: MLBr_01022

Alternate gene names: 221229969

Gene position: 1190949-1189225 (Counterclockwise)

Preceding gene: 221229970

Following gene: 221229968

Centisome position: 36.44

GC content: 60.58

Gene sequence:

>1725_bases
GTGGAACAGTCCAGGCGCGTGCCACCGTTACAATGGTTATCGGCGGTCGCCCGACCGATAGCGCGAGTACTCACGCTGAT
ATCAACGCCGACATTCGACATAGCAGACACTTTCGGTTATGCATGCCCAAACCCACCGGAAGTGAGTCCGACCGAAGGGG
TGTATGTGGTAGCGACCAAGACAAGTCCGGCAACCGGCCAGCCGGTGAAGCGCACCGCCACCAAGACGCCCGCGACCCCC
GCCAAGCGATCGACGACTAAGGCCGCAAATGGCTCCGCCCCCACAAAACGAGCCACTAGTACAGCATCTCGGGCCACCAA
ATCCACGACACCACCTAAAGCCTCCGGGGCAAGCGACGCTGCGAAGAAGGCCCGCACCTCGGTCAAAAGCGTCGATACAA
AGGCACCGTCCGCGCAGGACCACACGACTAAAGCCTCGGCAGCCAAAGACACCCTGAACGATCCCGAAGCCACTCTGAAC
ACCCTCGACTCCGACGCCACTGCCGACGACCTCGATGCCGAACCGGACCTCGACGTTGAGCCCAGCGAGGACATCGACAT
CGACATCGAAGCCGCCGACCTCGGTCTCGACGACCTCGAAGACGACGACGTAGTCACCGACATCGAACCAGGTGAAACCG
AGGATGGCGAAGCTACTGCAGCCACCAAGACCGACGAAGCTTCCACCGATGATGACGAAGAGATCGTTGAGCCGACCGAA
AAAGACAAGGCCTCCGGCGATTTCGTCTGGGACGAAGATGAATCCGAGGCGTTGCGTCAGGCCCGTAAGGATGCCGAGCT
CACCGCATCGGCAGACTCAGTTCGCGCGTACCTCAAACAGATCGGCAAGGTGGCCCTGCTCAACGCCGAGGAAGAGGTTG
AGCTGGCGAAGCGGATCGAGGCTGGCCTATACGCCACGCAGCTAATGACCGAGCTGTCTGAGCGGGGCACCAAACTACCC
ACCGCCCAGCGCCGCGACATGATGTGGATCTGTCGCGACGGCGATCGTGCGAAAAACCATCTGTTGGAAGCCAACCTGCG
GCTGGTGGTTTCGCTGGCCAAGCGCTACACCGGCCGCGGGATGGCGTTCCTCGACCTGATACAGGAAGGCAACCTGGGCC
TGATCCGGGCAGTCGAGAAGTTCGACTACACCAAGGGGTACAAGTTCTCGACGTACGCGACGTGGTGGATCCGCCAGGCC
ATCACCCGCGCCATGGCGGACCAAGCCCGCACCATCCGCATCCCGGTCCACATGGTCGAGGTGATCAACAAGCTCGGCCG
CATTCAGCGGGAACTACTACAGGATCTGGGCCGCGAGCCCACGCCTGAAGAGCTCGCAAAAGAGATGGACATCACACCTG
AGAAGGTGCTCGAGATCCAGCAATACGCTCGTGAACCAATCTCATTAGATCAGACCATCGGCGATGAGGGCGACAGCCAG
CTGGGCGACTTCATCGAAGATAGTGAAGCGGTGGTGGCCGTAGACGCGGTGTCGTTTACGTTGCTGCAAGACCAGTTGCA
GTCGGTGTTGGAAACACTCTCAGAACGCGAGGCCGGCGTAGTACGGCTTCGCTTCGGCCTCACTGACGGCCAGCCTCGCA
CCCTCGACGAAATCGGCCAGGTCTACGGCGTAACCCGCGAACGCATCCGTCAGATCGAGTCTAAGACGATGTCGAAGTTA
CGCCACCCCAGCCGTTCCCAGGTCTTGCGTGACTACCTTGACTAG

Upstream 100 bases:

>100_bases
AACCGCACCCCGATGGTGGCCGCAGCCCTGCAGAATACGGCCGGCATAGTCGGCGCTGCCATGGCTTCTACCGCAGATGT
GACGCACTGAATTTTGCCCG

Downstream 100 bases:

>100_bases
CAACACCCCGTAGAACACGTTCTTGCCATCCGCTCTTCGCCTTGTCATGAGGGGTCGATAGCCGTGACCGACAGCTCGTG
GTCCACGGCTGACTCCGTAC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSPTEGVYVVATKTSPATGQPVKRTATKTPATP
AKRSTTKAANGSAPTKRATSTASRATKSTTPPKASGASDAAKKARTSVKSVDTKAPSAQDHTTKASAAKDTLNDPEATLN
TLDSDATADDLDAEPDLDVEPSEDIDIDIEAADLGLDDLEDDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTE
KDKASGDFVWDEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTELSERGTKLP
TAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD

Sequences:

>Translated_574_residues
MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSPTEGVYVVATKTSPATGQPVKRTATKTPATP
AKRSTTKAANGSAPTKRATSTASRATKSTTPPKASGASDAAKKARTSVKSVDTKAPSAQDHTTKASAAKDTLNDPEATLN
TLDSDATADDLDAEPDLDVEPSEDIDIDIEAADLGLDDLEDDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTE
KDKASGDFVWDEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTELSERGTKLP
TAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD
>Mature_574_residues
MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSPTEGVYVVATKTSPATGQPVKRTATKTPATP
AKRSTTKAANGSAPTKRATSTASRATKSTTPPKASGASDAAKKARTSVKSVDTKAPSAQDHTTKASAAKDTLNDPEATLN
TLDSDATADDLDAEPDLDVEPSEDIDIDIEAADLGLDDLEDDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTE
KDKASGDFVWDEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTELSERGTKLP
TAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=60.2510460251046, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI1789098, Length=298, Percent_Identity=38.5906040268456, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=30.7359307359307, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 63039; Mature: 63039

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSPTEGVYVVATK
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
TSPATGQPVKRTATKTPATPAKRSTTKAANGSAPTKRATSTASRATKSTTPPKASGASDA
CCCCCCCCHHHHCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHH
AKKARTSVKSVDTKAPSAQDHTTKASAAKDTLNDPEATLNTLDSDATADDLDAEPDLDVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
PSEDIDIDIEAADLGLDDLEDDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTE
CCCCCEEEEEHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
KDKASGDFVWDEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHH
AGLYATQLMTELSERGTKLPTAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRG
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVE
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHH
VINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCHH
LGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQ
HHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHH
VYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MEQSRRVPPLQWLSAVARPIARVLTLISTPTFDIADTFGYACPNPPEVSPTEGVYVVATK
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEC
TSPATGQPVKRTATKTPATPAKRSTTKAANGSAPTKRATSTASRATKSTTPPKASGASDA
CCCCCCCCHHHHCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHH
AKKARTSVKSVDTKAPSAQDHTTKASAAKDTLNDPEATLNTLDSDATADDLDAEPDLDVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCC
PSEDIDIDIEAADLGLDDLEDDDVVTDIEPGETEDGEATAATKTDEASTDDDEEIVEPTE
CCCCCEEEEEHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
KDKASGDFVWDEDESEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHH
AGLYATQLMTELSERGTKLPTAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRG
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCC
MAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVE
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHH
VINKLGRIQRELLQDLGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCHH
LGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQ
HHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHH
VYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]