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The map label for this gene is lexA

Identifier: 221229956

GI number: 221229956

Start: 1175061

End: 1175768

Strand: Reverse

Name: lexA

Synonym: MLBr_01003

Alternate gene names: 221229956

Gene position: 1175768-1175061 (Counterclockwise)

Preceding gene: 221229960

Following gene: 221229954

Centisome position: 35.98

GC content: 60.73

Gene sequence:

>708_bases
ATGAGCGACAGCACCGACATATCAGGCATCACCGTCGACGGCCGCTTGCATTCTATGGATTCAGGGCTCACCGAGCGGCA
ACGCACCATCCTGAACGTCATCCGGGCCTCGGTAACCAGCCGCGGATACCCACCCAGCATCCGGGAAATCGCCGATGCTG
TCGGTCTGACGTCGACATCCTCGGTGGCTCACCAGCTGCGCACCCTTGAACGCAAGGGCTACTTGCGCCGTGACCCGAAT
CGCCCGCGAGCTGTCAATGTCCGCGGCGTCGAAGAGACTCAGGCAGCCGGCCCCGCTGTACTCACCGAAGTCGCTGGCTC
AGACGTCTTACCGGAGCCCACCTTTGTCCCGATCCTCGGACGCATCGCGGCCGGTAGTCCGATCTTCGCCGAAGGAACTG
TCGAGGACATCTTTCCGCTGCCCCGTGAACTGGTCGGCGAGGGTACGCTCTTTTTACTCAAGGTGACCGGCGACTCGATG
GTCGAAGCCGCGATCTGCGACGGTGACTGGGTGGTCGTACGGCAACAAAAAGTCGCCGACAACGGCGATATCGTCGCAGC
CATGATTGACGGTGAGGCCACCGTGAAGACGTTCAAACGCGCAGGCGGTCAGGTGTGGCTGATACCGCACAATCCAGCGT
TCGATCCCATTCCCGGCAACGATGCAACGGTGCTTGGCAAAGTCGTGACAGTAATCCGCAAGATATAA

Upstream 100 bases:

>100_bases
CGCAATACGGTCTTCTAGGCTGGCGGACGATCCCGCTCCCGCAACACATTTGTCGAACACATGTTTGATTCTCCACTGAC
ATCGGACTACATTCAGTGGC

Downstream 100 bases:

>100_bases
CCCCCGAAAAGTCTTGTGCGTAGTCGACCGGACCATCGTCAGTCGGCCTTAATAAAGCCGTTGACCTGGGCGATTTCTTC
ATTGGCTAGCCAGACTTCAG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTSSVAHQLRTLERKGYLRRDPN
RPRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILGRIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSM
VEAAICDGDWVVVRQQKVADNGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI

Sequences:

>Translated_235_residues
MSDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTSSVAHQLRTLERKGYLRRDPN
RPRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILGRIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSM
VEAAICDGDWVVVRQQKVADNGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI
>Mature_234_residues
SDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTSSVAHQLRTLERKGYLRRDPNR
PRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILGRIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSMV
EAAICDGDWVVVRQQKVADNGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=217, Percent_Identity=31.7972350230415, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_MYCLB (B8ZQU5)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503372.1
- ProteinModelPortal:   B8ZQU5
- MEROPS:   S24.001
- EnsemblBacteria:   EBMYCT00000086031
- GeneID:   7326732
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016631
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25089; Mature: 24958

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: ACT_SITE 159-159 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTS
CCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHH
SVAHQLRTLERKGYLRRDPNRPRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILG
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHCCCCCCCCCCHHHHHH
RIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSMVEAAICDGDWVVVRQQKVAD
HHHCCCCEEECCCHHHHHCCCHHHHCCCCEEEEEECCCCEEEEEEECCCEEEEEEEECCC
NGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI
CCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSTDISGITVDGRLHSMDSGLTERQRTILNVIRASVTSRGYPPSIREIADAVGLTSTS
CCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHH
SVAHQLRTLERKGYLRRDPNRPRAVNVRGVEETQAAGPAVLTEVAGSDVLPEPTFVPILG
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHCCCCCCCCCCHHHHHH
RIAAGSPIFAEGTVEDIFPLPRELVGEGTLFLLKVTGDSMVEAAICDGDWVVVRQQKVAD
HHHCCCCEEECCCHHHHHCCCHHHHCCCCEEEEEECCCCEEEEEEECCCEEEEEEEECCC
NGDIVAAMIDGEATVKTFKRAGGQVWLIPHNPAFDPIPGNDATVLGKVVTVIRKI
CCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA