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The map label for this gene is murD

Identifier: 221229909

GI number: 221229909

Start: 1080264

End: 1081736

Strand: Direct

Name: murD

Synonym: MLBr_00912

Alternate gene names: 221229909

Gene position: 1080264-1081736 (Clockwise)

Preceding gene: 221229908

Following gene: 221229910

Centisome position: 33.06

GC content: 64.15

Gene sequence:

>1473_bases
GTGCTTGACACACTGGTACCGGGCGCGCCTGTATTAGTAGCTGGTGGCGGGGTGACCGGGCGGGCGGTACTGGCGGCGCT
GACCCGTTTTGGCATGGCGGCAACGCTGTGTGACGACGACCCAGCCGCCCTGCAGCAATACGCCGACAACGGGGTGGCTA
CCGTGAGTGCAGCTACCGCCACACAACAGATGTTCGAGCGGGGAAGAAAATACGTCCTGGTGGTCACCAGTCCAGGTTTC
GCGCCGACCACCCCCGTGCTCGTCGCCGCGTCGGCGGCGCGGGTGCCTATTTGGGGCGATGTGGAGTTAGCTTGGCGACT
CGATGCCGCGGGCTACTACGGGCCGCCACGTCGCTGGCTTGTGGTGACGGGTACGAACGGTAAAACGACGACGACGTCAA
TGCTGCACGCGATGTTGGCGGCAGATAACCGGCGTAGCCTGCTGTGTGGAAACATCGGCCGCCCGGTGCTGGATGTCTTA
ACTGAGTTCGCCGAACCCTCGGATTTTCTTGCTGTCGAGCTGTCCAGTTTTCAGCTGCACTGGGCTCCTTCGCTACGGCC
GGAGGCCGGCGTGGTGCTCAACATTGCCGAAGACCACCTCGACTGGCATTCGACAATGGCCGATTACACCATGGCGAAGG
CTCGGGTGCTGACCGGCCGGGTGGCGGTGGTTGGGCTGGACGACAGCCGGGCCGCGGCACTGCTGAGCACGACAGTAGCG
CCGGTACGGGTCGGCTTCCGGTTCGGAGAACCAGCTGTGGGCGAGCTCGGGGTGCGTGACGGTTATCTCGTTGATCGTGC
GTTTGCTGAAGACCTGGCCTTAATGCCGGTTACGTCGATACCGGTGTCGGGCCCGGTCGGAGTTCTCGACGCGTTGGCCG
CTGCGGCACTGGCCCGCAGTGTCGGTGTGGGGGCGACCGCGATCGCCGACGCGGTCGCATTGTTTCGGCTGGGCCGACAC
CGGGCGGAGGTGGTAGCCGTTGCTGACGGGATCCGCTACGTTGATGATTCCAAGGCCACCAACCCGCACGCAGCTCTTGT
CTCGGTATTAGCCTACCCGCGTGTGGTGTGGGTGGCCGGTGGCCTACTCAAGGGTGCGTCTGTCGATGCGGAGGTTGCGC
GCATGGCGCCTCAGTTGGTCGGCGCGGTGCTGATCGGCCGAGATCGCGCGATGGTTGCCGAGGCGTTATCACGACACGCG
CCCAATGTCCCCGTTGTCCAGGTTGTGGCAGGCGAGGATGCTGGTATGCATGCGGTTGCCGTTGTTTCTGGTACTGATGT
GATAAGCATCAGTGATGTTGGCGGGACTATCGGTACTCGCGTGATGGTTGCGGCCGTGGCGGCGGCCCGGGATTTAGCCC
AACCTGGTGACACCGTACTGCTGGCACCGGCCGGCGCGTCGTTCGACCAATTTAGCGGTTATGCCGACCGCGGTGACACA
TTCGCAACTGCCGTGCGCGCAGCGATCCGGTAG

Upstream 100 bases:

>100_bases
ACCACTGTGATCATTCGTTTTTGGCTGCTCACAGCGATCGCTTGCGGTCTGGGCGTGGTCCTGTTCTATGGCGAGTGGCT
AGCCACCATTGGCGCTTGAC

Downstream 100 bases:

>100_bases
GTGGGCGTGGGTAACGCGCTGGGTCGGGATAGGGGTCGGGGCACGGGAGATAACAACAGTGTGCAGGCCGCCACGACCGT
GGAGGCGGTTGAGCCCGGCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MLDTLVPGAPVLVAGGGVTGRAVLAALTRFGMAATLCDDDPAALQQYADNGVATVSAATATQQMFERGRKYVLVVTSPGF
APTTPVLVAASAARVPIWGDVELAWRLDAAGYYGPPRRWLVVTGTNGKTTTTSMLHAMLAADNRRSLLCGNIGRPVLDVL
TEFAEPSDFLAVELSSFQLHWAPSLRPEAGVVLNIAEDHLDWHSTMADYTMAKARVLTGRVAVVGLDDSRAAALLSTTVA
PVRVGFRFGEPAVGELGVRDGYLVDRAFAEDLALMPVTSIPVSGPVGVLDALAAAALARSVGVGATAIADAVALFRLGRH
RAEVVAVADGIRYVDDSKATNPHAALVSVLAYPRVVWVAGGLLKGASVDAEVARMAPQLVGAVLIGRDRAMVAEALSRHA
PNVPVVQVVAGEDAGMHAVAVVSGTDVISISDVGGTIGTRVMVAAVAAARDLAQPGDTVLLAPAGASFDQFSGYADRGDT
FATAVRAAIR

Sequences:

>Translated_490_residues
MLDTLVPGAPVLVAGGGVTGRAVLAALTRFGMAATLCDDDPAALQQYADNGVATVSAATATQQMFERGRKYVLVVTSPGF
APTTPVLVAASAARVPIWGDVELAWRLDAAGYYGPPRRWLVVTGTNGKTTTTSMLHAMLAADNRRSLLCGNIGRPVLDVL
TEFAEPSDFLAVELSSFQLHWAPSLRPEAGVVLNIAEDHLDWHSTMADYTMAKARVLTGRVAVVGLDDSRAAALLSTTVA
PVRVGFRFGEPAVGELGVRDGYLVDRAFAEDLALMPVTSIPVSGPVGVLDALAAAALARSVGVGATAIADAVALFRLGRH
RAEVVAVADGIRYVDDSKATNPHAALVSVLAYPRVVWVAGGLLKGASVDAEVARMAPQLVGAVLIGRDRAMVAEALSRHA
PNVPVVQVVAGEDAGMHAVAVVSGTDVISISDVGGTIGTRVMVAAVAAARDLAQPGDTVLLAPAGASFDQFSGYADRGDT
FATAVRAAIR
>Mature_490_residues
MLDTLVPGAPVLVAGGGVTGRAVLAALTRFGMAATLCDDDPAALQQYADNGVATVSAATATQQMFERGRKYVLVVTSPGF
APTTPVLVAASAARVPIWGDVELAWRLDAAGYYGPPRRWLVVTGTNGKTTTTSMLHAMLAADNRRSLLCGNIGRPVLDVL
TEFAEPSDFLAVELSSFQLHWAPSLRPEAGVVLNIAEDHLDWHSTMADYTMAKARVLTGRVAVVGLDDSRAAALLSTTVA
PVRVGFRFGEPAVGELGVRDGYLVDRAFAEDLALMPVTSIPVSGPVGVLDALAAAALARSVGVGATAIADAVALFRLGRH
RAEVVAVADGIRYVDDSKATNPHAALVSVLAYPRVVWVAGGLLKGASVDAEVARMAPQLVGAVLIGRDRAMVAEALSRHA
PNVPVVQVVAGEDAGMHAVAVVSGTDVISISDVGGTIGTRVMVAAVAAARDLAQPGDTVLLAPAGASFDQFSGYADRGDT
FATAVRAAIR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=414, Percent_Identity=30.6763285024155, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_MYCLB (B8ZQP8)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503325.1
- ProteinModelPortal:   B8ZQP8
- SMR:   B8ZQP8
- EnsemblBacteria:   EBMYCT00000085580
- GeneID:   7324927
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015779
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK03369
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50583; Mature: 50583

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTLVPGAPVLVAGGGVTGRAVLAALTRFGMAATLCDDDPAALQQYADNGVATVSAATA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCEEEEHHHH
TQQMFERGRKYVLVVTSPGFAPTTPVLVAASAARVPIWGDVELAWRLDAAGYYGPPRRWL
HHHHHHCCCEEEEEEECCCCCCCCHHEEEECCCCCCEECCEEEEEEEECCCCCCCCCEEE
VVTGTNGKTTTTSMLHAMLAADNRRSLLCGNIGRPVLDVLTEFAEPSDFLAVELSSFQLH
EEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECEEEE
WAPSLRPEAGVVLNIAEDHLDWHSTMADYTMAKARVLTGRVAVVGLDDSRAAALLSTTVA
ECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEECEEEEEECCCCHHHHHHHHHHH
PVRVGFRFGEPAVGELGVRDGYLVDRAFAEDLALMPVTSIPVSGPVGVLDALAAAALARS
HHEEEEECCCCCCCCCCCCCCCEEHHHHHCCCEECEEECCCCCCCHHHHHHHHHHHHHHH
VGVGATAIADAVALFRLGRHRAEVVAVADGIRYVDDSKATNPHAALVSVLAYPRVVWVAG
CCCCHHHHHHHHHHHHHCCCHHEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHEEHHC
GLLKGASVDAEVARMAPQLVGAVLIGRDRAMVAEALSRHAPNVPVVQVVAGEDAGMHAVA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
VVSGTDVISISDVGGTIGTRVMVAAVAAARDLAQPGDTVLLAPAGASFDQFSGYADRGDT
EEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCH
FATAVRAAIR
HHHHHHHHCC
>Mature Secondary Structure
MLDTLVPGAPVLVAGGGVTGRAVLAALTRFGMAATLCDDDPAALQQYADNGVATVSAATA
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCEEEEHHHH
TQQMFERGRKYVLVVTSPGFAPTTPVLVAASAARVPIWGDVELAWRLDAAGYYGPPRRWL
HHHHHHCCCEEEEEEECCCCCCCCHHEEEECCCCCCEECCEEEEEEEECCCCCCCCCEEE
VVTGTNGKTTTTSMLHAMLAADNRRSLLCGNIGRPVLDVLTEFAEPSDFLAVELSSFQLH
EEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECEEEE
WAPSLRPEAGVVLNIAEDHLDWHSTMADYTMAKARVLTGRVAVVGLDDSRAAALLSTTVA
ECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEECEEEEEECCCCHHHHHHHHHHH
PVRVGFRFGEPAVGELGVRDGYLVDRAFAEDLALMPVTSIPVSGPVGVLDALAAAALARS
HHEEEEECCCCCCCCCCCCCCCEEHHHHHCCCEECEEECCCCCCCHHHHHHHHHHHHHHH
VGVGATAIADAVALFRLGRHRAEVVAVADGIRYVDDSKATNPHAALVSVLAYPRVVWVAG
CCCCHHHHHHHHHHHHHCCCHHEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHEEHHC
GLLKGASVDAEVARMAPQLVGAVLIGRDRAMVAEALSRHAPNVPVVQVVAGEDAGMHAVA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
VVSGTDVISISDVGGTIGTRVMVAAVAAARDLAQPGDTVLLAPAGASFDQFSGYADRGDT
EEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCH
FATAVRAAIR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA