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The map label for this gene is gpsI

Identifier: 221229857

GI number: 221229857

Start: 1013882

End: 1016203

Strand: Direct

Name: gpsI

Synonym: MLBr_00854

Alternate gene names: 221229857

Gene position: 1013882-1016203 (Clockwise)

Preceding gene: 221229856

Following gene: 221229858

Centisome position: 31.02

GC content: 61.02

Gene sequence:

>2322_bases
ATGTCTGTAGCTGAAATCGAAGAGGGCGTGTTCGAGGCAACCGTTACTATCGATAACGGGAGTTTCGGCACCCGCGCCAT
CCGTTTTGAGACCGGGCGCTTAGCCTTGCAAGCCGCTGGCGCAGTGGTGGCCTACCTGGACGCCGACAATATGCTCTTGT
CGGCGACCACCGCCAGCAAGAACCCCAAGGAGCAATTCGACTTCTTCCCGCTTACGGTCGATGTTGAGGAGCGGATGTAT
GCTGCCGGTAGGATCCCTGGTTCGTTCTTCCGCCGTGAGGGTCGGCCGTCCACCGACGCGATCCTTACCTGCCGGCTCAT
CGATCGCCCTTTGCGCCCGTCTTTTGTCGACGGCCTGCGCAACGAGATCCAGGTCGTGGTGACGATTCTGAGCGTGGATC
CCAACGATCTCTATGATGTGTTGGCCATCAATGCTGCCTCGGCCTCCACTCAGCTGGGCGGTCTACCGTTCTCCGGGCCT
ATCGGCGGTGTGCGGGTGGCGCTCATCGACGGTACCTGGGTTGCTTTCCCCACTGTAGAGCAGCTCGAGCGGGCCGTGTT
CGACATGGTGATTGCCGGCCGGATTGTTGGCACGGATGACGAGGGCAAGCCTGATGTCGCTATCATGATGGTCGAGGCTG
AGGCTACCGAAAACGTCATCGAGCTGGTCGAGTGCGGTGCTCAGGCGCCGACGGAAAGCATCGTGGCCCAAGGACTCGAG
GTGGCCAAGCCGTTCATTGCTGCGCTGTGTACCGCGCAGCAGGAACTTGCTGACGTTGTGTCTTCTCGCCAAGCCAAACC
GCCAGTCGAGTACCCGACGTTCCCCGACTACGGCGACGACGTCTACTACTCGGTGGCTTCGATGGCTACCGATGAGTTGG
CCGCGGCCTTGACCATCGGCGCCAAGCTTGAGCGCGATCAACGCACCAACGAGATCAAGACCCAGGTTCTCGAGCGGCTC
GCCGGCACCTACGAGGGCCGGGAAAAGGAGTTGGGCGCAGCATTCCGTTCGTTGACCAAGAAACTGGTCCGACAGCGGAT
CCTCACCAACCATTTCCGTATCGACGGCCGCGGGATCACCGACATTCGCGCGTTGTCGGCCGAAGTTGCGGTGGTTCCGC
GGGCGCACGGTAGCGCGCTGTTCGAGCGCGGCGAAACCCAGATCCTTGGCGTGACCACGCTCGACATGGTCAAGATGGCT
CAGCAGATCGACTCGCTGGGGCCAGAAACATCCAAGCGGTATATGCACCACTACAACTTCCCGCCGTTCTCCACTGGTGA
GACTGGTCGAGTCGGCTCGCCCAAGCGGCGTGAGATCGGGCACGGTGCGCTGGCTGAGCGGGCTCTAGTGCCGGTGCTAC
CCAGCGTCGAGGAGTTCCCGTACGCCATCCGTCAGGTGTCCGAGGCGCTGGGTTCTAACGGCTCGACCTCGATGGGGTCG
GTTTGCGCTTCCACGCTGGCGCTGCTCAACGCCGGCGTGCCGCTCAAGGCGCCGGTGGCTGGCATTGCTATGGGGCTGGT
CTCCGACGATGTTGAGTTTGACGGAGGGACTGAGCGTCGTTTTGTCACCTTGACCGACATCCTGGGCGCCGAAGACGCCT
TCGGCGACATGGATTTTAAATGCGCCGGTACCAAGGACTTTGTCACCGCGCTGCAGCTCGATACCAAACTTGACGGTATT
CCCTCCCAGGTGCTCGCGGGTGCGCTTGCGCAGGCCAAAGACGCCCGGCTGACCATCCTCGAGGTCATGGCTGAGGCCAT
TGACAGACCCGACGAGATGAGCCCGTATGCGCCGCGGGTCATCACTATCAAGGTTCCAGTGGATAAGATCGGTGAAGTCA
TCGGCCCCAAGGGCAAGGTCATCAACGCCATTACCGAGGAGACCGGTGCGCAGATTTCTATCGAGGATGACGGTACTGTG
TTCGTCGGTGCGACCGATGGCCTGTCGGCGCAGGCCGCGATCAATAAGATTAATGCCATCGCGAACCCGCAGCTGCCGAC
GGTCGGCGAACGGTTCCTTGGAACTGTCGTAAAGATAACGGAATTCGGCGCTTTCGTGTCGCTGCTGCCTGGGCGTGACG
GTTTGGTTCACATCTCCAAGCTTGGCAAGGGTAAGCGCATCGCAAAGGTGGAAGACGTCGTGAACGTCGGTGATAAGTTG
CGCGTCGAGATCGCAGATATCGAAAAACGCGGCAAGATCTCATTGGTCTTGGTGGCTGACGACGATTCGGCCACCGTCCC
TGCAGCTCCGGCCGATGCTGGGGCTGCTCAGGAGTTCGGTTCGGGTACTGCGCCAGCCGATGCTGCGACGGCCAGTAGCT
GA

Upstream 100 bases:

>100_bases
GCTTCGATCGATGGCCGTAGTCGTATTCAGACTGTGCTCTCCGGGATTGCGTGCGTGGCGCCGAAACAGATTAATAACGA
GAGAGGCTGTGCGGACGGTT

Downstream 100 bases:

>100_bases
CCCCGGAGCTAAGGGTAAGGGTGCCTTGCGGCGGAGCAAGCAGGGCGCCGAGGGGAAGGCGGAAAAGAAGGCCGCACGAT
CGGCTGGAGTGTGTCGCACC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MSVAEIEEGVFEATVTIDNGSFGTRAIRFETGRLALQAAGAVVAYLDADNMLLSATTASKNPKEQFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSVDPNDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVAFPTVEQLERAVFDMVIAGRIVGTDDEGKPDVAIMMVEAEATENVIELVECGAQAPTESIVAQGLE
VAKPFIAALCTAQQELADVVSSRQAKPPVEYPTFPDYGDDVYYSVASMATDELAAALTIGAKLERDQRTNEIKTQVLERL
AGTYEGREKELGAAFRSLTKKLVRQRILTNHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMA
QQIDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGS
VCASTLALLNAGVPLKAPVAGIAMGLVSDDVEFDGGTERRFVTLTDILGAEDAFGDMDFKCAGTKDFVTALQLDTKLDGI
PSQVLAGALAQAKDARLTILEVMAEAIDRPDEMSPYAPRVITIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTV
FVGATDGLSAQAAINKINAIANPQLPTVGERFLGTVVKITEFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKL
RVEIADIEKRGKISLVLVADDDSATVPAAPADAGAAQEFGSGTAPADAATASS

Sequences:

>Translated_773_residues
MSVAEIEEGVFEATVTIDNGSFGTRAIRFETGRLALQAAGAVVAYLDADNMLLSATTASKNPKEQFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSVDPNDLYDVLAINAASASTQLGGLPFSGP
IGGVRVALIDGTWVAFPTVEQLERAVFDMVIAGRIVGTDDEGKPDVAIMMVEAEATENVIELVECGAQAPTESIVAQGLE
VAKPFIAALCTAQQELADVVSSRQAKPPVEYPTFPDYGDDVYYSVASMATDELAAALTIGAKLERDQRTNEIKTQVLERL
AGTYEGREKELGAAFRSLTKKLVRQRILTNHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMA
QQIDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGS
VCASTLALLNAGVPLKAPVAGIAMGLVSDDVEFDGGTERRFVTLTDILGAEDAFGDMDFKCAGTKDFVTALQLDTKLDGI
PSQVLAGALAQAKDARLTILEVMAEAIDRPDEMSPYAPRVITIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTV
FVGATDGLSAQAAINKINAIANPQLPTVGERFLGTVVKITEFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKL
RVEIADIEKRGKISLVLVADDDSATVPAAPADAGAAQEFGSGTAPADAATASS
>Mature_772_residues
SVAEIEEGVFEATVTIDNGSFGTRAIRFETGRLALQAAGAVVAYLDADNMLLSATTASKNPKEQFDFFPLTVDVEERMYA
AGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQVVVTILSVDPNDLYDVLAINAASASTQLGGLPFSGPI
GGVRVALIDGTWVAFPTVEQLERAVFDMVIAGRIVGTDDEGKPDVAIMMVEAEATENVIELVECGAQAPTESIVAQGLEV
AKPFIAALCTAQQELADVVSSRQAKPPVEYPTFPDYGDDVYYSVASMATDELAAALTIGAKLERDQRTNEIKTQVLERLA
GTYEGREKELGAAFRSLTKKLVRQRILTNHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQ
QIDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSV
CASTLALLNAGVPLKAPVAGIAMGLVSDDVEFDGGTERRFVTLTDILGAEDAFGDMDFKCAGTKDFVTALQLDTKLDGIP
SQVLAGALAQAKDARLTILEVMAEAIDRPDEMSPYAPRVITIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVF
VGATDGLSAQAAINKINAIANPQLPTVGERFLGTVVKITEFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKLR
VEIADIEKRGKISLVLVADDDSATVPAAPADAGAAQEFGSGTAPADAATASS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=696, Percent_Identity=35.7758620689655, Blast_Score=381, Evalue=1e-105,
Organism=Escherichia coli, GI145693187, Length=717, Percent_Identity=44.6304044630404, Blast_Score=554, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI115534063, Length=734, Percent_Identity=32.016348773842, Blast_Score=311, Evalue=9e-85,
Organism=Drosophila melanogaster, GI281362905, Length=727, Percent_Identity=36.0385144429161, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24651641, Length=727, Percent_Identity=36.0385144429161, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24651643, Length=727, Percent_Identity=36.0385144429161, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=36.1573373676248, Blast_Score=354, Evalue=2e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_MYCLB (B8ZQJ6)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503273.1
- ProteinModelPortal:   B8ZQJ6
- SMR:   B8ZQJ6
- EnsemblBacteria:   EBMYCT00000086467
- GeneID:   7325955
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017412
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82048; Mature: 81917

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAEIEEGVFEATVTIDNGSFGTRAIRFETGRLALQAAGAVVAYLDADNMLLSATTASK
CCHHHHHCCCEEEEEEEECCCCCCEEEEEECCCEEEEECCEEEEEEECCCEEEEEECCCC
NPKEQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEEECCCCCCCHHHHHHH
NEIQVVVTILSVDPNDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVAFPTVE
HCEEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHH
QLERAVFDMVIAGRIVGTDDEGKPDVAIMMVEAEATENVIELVECGAQAPTESIVAQGLE
HHHHHHHHHHHHCEEECCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHH
VAKPFIAALCTAQQELADVVSSRQAKPPVEYPTFPDYGDDVYYSVASMATDELAAALTIG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKLERDQRTNEIKTQVLERLAGTYEGREKELGAAFRSLTKKLVRQRILTNHFRIDGRGIT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQQIDSLGPETSKRYMHHYNF
HHHHHCCEEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGS
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
VCASTLALLNAGVPLKAPVAGIAMGLVSDDVEFDGGTERRFVTLTDILGAEDAFGDMDFK
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCEE
CAGTKDFVTALQLDTKLDGIPSQVLAGALAQAKDARLTILEVMAEAIDRPDEMSPYAPRV
ECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
ITIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVGATDGLSAQAAINKINAI
EEEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHH
ANPQLPTVGERFLGTVVKITEFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKL
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
RVEIADIEKRGKISLVLVADDDSATVPAAPADAGAAQEFGSGTAPADAATASS
EEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVAEIEEGVFEATVTIDNGSFGTRAIRFETGRLALQAAGAVVAYLDADNMLLSATTASK
CHHHHHCCCEEEEEEEECCCCCCEEEEEECCCEEEEECCEEEEEEECCCEEEEEECCCC
NPKEQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLR
CCHHHCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEEECCCCCCCHHHHHHH
NEIQVVVTILSVDPNDLYDVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVAFPTVE
HCEEEEEEEEECCCCHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHH
QLERAVFDMVIAGRIVGTDDEGKPDVAIMMVEAEATENVIELVECGAQAPTESIVAQGLE
HHHHHHHHHHHHCEEECCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHH
VAKPFIAALCTAQQELADVVSSRQAKPPVEYPTFPDYGDDVYYSVASMATDELAAALTIG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKLERDQRTNEIKTQVLERLAGTYEGREKELGAAFRSLTKKLVRQRILTNHFRIDGRGIT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
DIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQQIDSLGPETSKRYMHHYNF
HHHHHCCEEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGS
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
VCASTLALLNAGVPLKAPVAGIAMGLVSDDVEFDGGTERRFVTLTDILGAEDAFGDMDFK
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCEE
CAGTKDFVTALQLDTKLDGIPSQVLAGALAQAKDARLTILEVMAEAIDRPDEMSPYAPRV
ECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
ITIKVPVDKIGEVIGPKGKVINAITEETGAQISIEDDGTVFVGATDGLSAQAAINKINAI
EEEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHH
ANPQLPTVGERFLGTVVKITEFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVNVGDKL
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEE
RVEIADIEKRGKISLVLVADDDSATVPAAPADAGAAQEFGSGTAPADAATASS
EEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA