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The map label for this gene is cysK

Identifier: 221229847

GI number: 221229847

Start: 996399

End: 997331

Strand: Reverse

Name: cysK

Synonym: MLBr_00839

Alternate gene names: 221229847

Gene position: 997331-996399 (Counterclockwise)

Preceding gene: 221229848

Following gene: 221229846

Centisome position: 30.52

GC content: 59.7

Gene sequence:

>933_bases
ATGAGCATTGCTGAAGACGTCACACAACTAGTTGGTAACACGCCACTGGTCCGGCTACGCCGAGTTACCGAAGGTGCGCT
GGCTGACGTTGTCGCCAAGCTGGAATCCTTCAATCCCGCCAACAGCGTGAAGGACAGGATAGGCGTGGCCATGATCGACA
CGGCCGAGGAAGCGGGTTTGATCAAGCCGGACACCGTCATCCTTGAGCCGACGAGCGGGAACACCGGCATCGCACTAGCT
ATGGTATGCGCAGCACGTGGCTACCACTGCGTGCTGACCATGCCCGAGACAATGAGCATCGAACGACGAATGTTGTTGCG
AGCGTACGGCGCCGAACTCATCTTGACTCCGGGCGCCGATGGCATGTCAGGAGCCATCGCCAAGGCTGAGGAATTAGCCA
AGTCCGATAAAAGGTATTTTATACCACAGCAATTCGAGAACCCAGCGAACCCAGCCATCCACGCCGCCACAACCGCCGAG
GAAGTGTGGCGCGACACCGATGGCAAGGTAGACATCTTCGTTTCTGGAATCGGGACCGGAGGCACTATCACTGGTGTTGC
CCGAATCATTAAAGAACGCCGGCCCGCCACGCAGTTCGTCGCCGTAGAGCCCGCCGCGTCGCCGGTGTTATCCGGTGGCC
AGAAGGGTCCTCACCCCATTCAGGGCATCGGTGCTGGATTCGTCCCGTCGGTGCTGGTCCTGGACCTGGTGGACGAGATC
GTTACCGTCGGCAACGAAGAAGCGCTCGACCTAGCCCGCAGACTGGCTAAGGAAGAGGGACTGCTGGTCGGCATCTCCGC
AGGGGCGGCCGTTTGCGCCGCCCTGCACGTGGCCCGCCGGCCAGAGAATACAGGAAAACTGATCGTCGTCGTGCTGCCCG
ATTTCGGCGAGCGATATTTAAGCACGATGCTATTCGCGGACTTAGCCAACTAG

Upstream 100 bases:

>100_bases
TAGCCATCCCCACTGAAATGGCCATAGAGTTCGACATTAGCTGGGAGGCCAATGAGGCTGAGATGTTTGAAGAAGCCGAG
CCCCTTGGAGATGAAGATAG

Downstream 100 bases:

>100_bases
CCATGTTTGCGGCAATCCGGCGTGATATCCAGGCAGCAAGACAGCGAGATCCGGCACAGCCCACGGTGCTGGAGGTCATC
TGCTGCTACCCAGGCGTGCA

Product: putative cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSIAEDVTQLVGNTPLVRLRRVTEGALADVVAKLESFNPANSVKDRIGVAMIDTAEEAGLIKPDTVILEPTSGNTGIALA
MVCAARGYHCVLTMPETMSIERRMLLRAYGAELILTPGADGMSGAIAKAEELAKSDKRYFIPQQFENPANPAIHAATTAE
EVWRDTDGKVDIFVSGIGTGGTITGVARIIKERRPATQFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPSVLVLDLVDEI
VTVGNEEALDLARRLAKEEGLLVGISAGAAVCAALHVARRPENTGKLIVVVLPDFGERYLSTMLFADLAN

Sequences:

>Translated_310_residues
MSIAEDVTQLVGNTPLVRLRRVTEGALADVVAKLESFNPANSVKDRIGVAMIDTAEEAGLIKPDTVILEPTSGNTGIALA
MVCAARGYHCVLTMPETMSIERRMLLRAYGAELILTPGADGMSGAIAKAEELAKSDKRYFIPQQFENPANPAIHAATTAE
EVWRDTDGKVDIFVSGIGTGGTITGVARIIKERRPATQFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPSVLVLDLVDEI
VTVGNEEALDLARRLAKEEGLLVGISAGAAVCAALHVARRPENTGKLIVVVLPDFGERYLSTMLFADLAN
>Mature_309_residues
SIAEDVTQLVGNTPLVRLRRVTEGALADVVAKLESFNPANSVKDRIGVAMIDTAEEAGLIKPDTVILEPTSGNTGIALAM
VCAARGYHCVLTMPETMSIERRMLLRAYGAELILTPGADGMSGAIAKAEELAKSDKRYFIPQQFENPANPAIHAATTAEE
VWRDTDGKVDIFVSGIGTGGTITGVARIIKERRPATQFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPSVLVLDLVDEIV
TVGNEEALDLARRLAKEEGLLVGISAGAAVCAALHVARRPENTGKLIVVVLPDFGERYLSTMLFADLAN

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=40.2555910543131, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=40.2555910543131, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=40.2555910543131, Blast_Score=228, Evalue=4e-60,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=57.7777777777778, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=43.1438127090301, Blast_Score=233, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17535051, Length=301, Percent_Identity=53.8205980066445, Blast_Score=323, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=50.8090614886731, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=52.5974025974026, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=51.505016722408, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17561720, Length=307, Percent_Identity=49.8371335504886, Blast_Score=267, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI32566672, Length=217, Percent_Identity=51.6129032258064, Blast_Score=223, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=39.6284829721362, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=35.6037151702786, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=47.1074380165289, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71996324, Length=315, Percent_Identity=26.3492063492064, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=36.9905956112853, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=35.3474320241692, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24643623, Length=314, Percent_Identity=40.4458598726115, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI20129101, Length=314, Percent_Identity=40.4458598726115, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSK_MYCLE (O32978)

Other databases:

- EMBL:   Z98741
- EMBL:   AL583919
- PIR:   T44912
- RefSeq:   NP_301633.1
- ProteinModelPortal:   O32978
- SMR:   O32978
- EnsemblBacteria:   EBMYCT00000028183
- GeneID:   909810
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0839
- NMPDR:   fig|272631.1.peg.505
- Leproma:   ML0839
- GeneTree:   EBGT00050000015764
- HOGENOM:   HBG748215
- OMA:   ETIGDTP
- ProtClustDB:   CLSK799368
- BioCyc:   MLEP272631:ML0839-MONOMER
- BRENDA:   2.5.1.47
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01139
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.47

Molecular weight: Translated: 32661; Mature: 32530

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 74-74 BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAEDVTQLVGNTPLVRLRRVTEGALADVVAKLESFNPANSVKDRIGVAMIDTAEEAGL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECHHHCCC
IKPDTVILEPTSGNTGIALAMVCAARGYHCVLTMPETMSIERRMLLRAYGAELILTPGAD
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMSGAIAKAEELAKSDKRYFIPQQFENPANPAIHAATTAEEVWRDTDGKVDIFVSGIGTG
CCHHHHHHHHHHHHCCCCEECCHHCCCCCCCCEEEHHHHHHHHCCCCCCEEEEEEECCCC
GTITGVARIIKERRPATQFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPSVLVLDLVDEI
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
VTVGNEEALDLARRLAKEEGLLVGISAGAAVCAALHVARRPENTGKLIVVVLPDFGERYL
HHCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
STMLFADLAN
HHHHHHHHCC
>Mature Secondary Structure 
SIAEDVTQLVGNTPLVRLRRVTEGALADVVAKLESFNPANSVKDRIGVAMIDTAEEAGL
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECHHHCCC
IKPDTVILEPTSGNTGIALAMVCAARGYHCVLTMPETMSIERRMLLRAYGAELILTPGAD
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMSGAIAKAEELAKSDKRYFIPQQFENPANPAIHAATTAEEVWRDTDGKVDIFVSGIGTG
CCHHHHHHHHHHHHCCCCEECCHHCCCCCCCCEEEHHHHHHHHCCCCCCEEEEEEECCCC
GTITGVARIIKERRPATQFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPSVLVLDLVDEI
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
VTVGNEEALDLARRLAKEEGLLVGISAGAAVCAALHVARRPENTGKLIVVVLPDFGERYL
HHCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
STMLFADLAN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002