Click here to switch to the map view.

The map label for this gene is dnaG

Identifier: 221229843

GI number: 221229843

Start: 988501

End: 990429

Strand: Direct

Name: dnaG

Synonym: MLBr_00833

Alternate gene names: 221229843

Gene position: 988501-990429 (Clockwise)

Preceding gene: 221229842

Following gene: 221229845

Centisome position: 30.25

GC content: 59.15

Gene sequence:

>1929_bases
ATGGTCGGCCGTATCCCAGATCGCGATATTGCCGCTATCCGTGAACGGGTCTGCATTGAGGAAGTCGTCGGCGATTACGT
CCAATTGCGGCGCGCCGGAGCCGATTCGCTTAAAGGCCTGTGTCCGTTCCACAACGAGAAATCACCGTCGTTTCATGTAC
GCCCAAACCATGGCCATTTCCACTGCTTTGGCTGTAGTGAGGGCGGTGACGTGTATGCGTTTATCCAGAAGATCGAACAC
GTCAGCTTTGTTGAGGCGGTTGAAATGCTGGCTGACCGGATTGGCCACACGATTAACTATTTCGGCACGGCCACCAGCGT
GCAGCGCGATCGCGGTAGTCGCAGCAGGCTCATTGCGGCCAATGCGGCCGCAGCAGCGTTCTATGCGGCAGCCTTGGAGT
CAGACGAGGCGGCACCGGCTCGCCAGTACCTCATGGAGCGCAATTTCGATGCTAATGCCGCACGTCATTTCGGCTGCGGA
TTCGCGCCGTCGGGCTGGGATTCATTGACAAAATATCTGATAAGCAAGGGTTTTGAATTCACAGAACTGGAGGCTGCGGG
GCTGTCTCGGCAGGGACGCCGTGGTCCGATGGACCGCTTTCACCGTCGGCTACTGTGGCCGATCCTCACATTGGCTGGCG
AAGTGATCGGGTTCGGCGCCCGGCGGTTGTTTGACGATGACCCGATGGAAGCCAAGTACGTCAACACACCCGAGACGCTG
TTGTATAAGAAGTCGTCGGTGATGTTTGGCATCGACTTGGCTAAACGGGATATCGTCAAGGGGCATCAGGCCGTTGTGGT
CGAGGGCTACACCGACGTGATGGCTATGCATCTGGCAGGGGTTACCACTGCTGTCGCGTCGTGCGGCACGGCGTTCGGCG
ATGAGCATCTGGCGATGCTGCGCAGGCTAATAATGGATGATAACTGGTATCGGGGTGAATTGATCTTTGTCTTCGATGGC
GATGCGGCTGGGCAGGCAGCGGCTCTGAAGGCATTCGACGGCGACCAGCAGGTTGCCGGAAAATCGTTCGTCGCCGTCGC
TGCGGATGGCATGGATCCGTGCGACCTCCGGCTCAGCTCTGGAGACCAGGCATTACGCGACTTGGTTGCTTGCCGCAAAC
CAATGTTCGAATTCGCTATTCGAAGTTTGATTCCTGACGGAATGGTTCTTGACGATGACCCACAGGCGAAAGTGGATGCG
CTGCGCCGATCCGTTCCGCTGGTTGCTCGGATTAGGGACTATGCGTTGCGCGACGAATACGCGCGACGCCTGGCGGGCTG
GACGGGATGGTCGGACGAAGGGCAAGTGCTTGCTCGAGTGCGCGAAGAGGCTAACCGACGTAGCGTGCCGGAACGCACCA
GAAGGCGCAGCGTGAGCGTTGAACAGTCACCATTCATGCAGCCGCCGGGTGCTCCGGCCGATCAACTCGCCGCCCGTCCT
GACCCGCGTGACCCAACACTGTGGCCGCAGCGTGAGGCTTTGAAATCGGTTTTGCAGTACCCAGTTCTGGCGGGCCCAGT
ATTCGACACACTTACTGTCGAGAGTTTCACTCATCCCGGCTACGGAGCCGTTCGCGCGGCCATTGACGCGGCGGGCGGGG
CGTCGGCCGGCATCACCGGAGCACAATGGATCGACGTGGTGCGCCAGCAAACCACATCGGCGCTGATCGCCGGTCTGATC
AATGAGCTAGGTGTGGAGTCGATCCAGGTCGACGACGAGAAGCTGCCGCGCTATATCGATGGGGTGCTGGCTCGCTTGCA
GGAAGTGTGGATGGGCCGACAGATTGCCGAGGTGAAATCCAAGTTGCAGCGTATGTCGCCGATTGACCAGGGTGACGAAT
ATCATGAACTGTTCGGCGATCTGGTCGCCATGGAAGCTTATCGACGCAGCCTGTTGGAGCAAGCCAGCGGCGACGATATG
ACCGCCTGA

Upstream 100 bases:

>100_bases
GGAGCGGATCGCATGCGGTGTCATTGTCCCGGCTTACTGAACTAGCCCGGAGCGGTGTTGGCTATGCAGCAAGCTGAACA
CGGGGTTCTAGACTGAGCCT

Downstream 100 bases:

>100_bases
GGTAGTGTTGGCTTTCAGTTTCCGCCGTTCTCCCGCCACTCGGCAGAGCGTTCGATAACAGTGGTCTGGTTATCGATTTC
AGCCAGGGTCACAAACCCCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MVGRIPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCSEGGDVYAFIQKIEH
VSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAANAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCG
FAPSGWDSLTKYLISKGFEFTELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETL
LYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLIMDDNWYRGELIFVFDG
DAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSSGDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDA
LRRSVPLVARIRDYALRDEYARRLAGWTGWSDEGQVLARVREEANRRSVPERTRRRSVSVEQSPFMQPPGAPADQLAARP
DPRDPTLWPQREALKSVLQYPVLAGPVFDTLTVESFTHPGYGAVRAAIDAAGGASAGITGAQWIDVVRQQTTSALIAGLI
NELGVESIQVDDEKLPRYIDGVLARLQEVWMGRQIAEVKSKLQRMSPIDQGDEYHELFGDLVAMEAYRRSLLEQASGDDM
TA

Sequences:

>Translated_642_residues
MVGRIPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCSEGGDVYAFIQKIEH
VSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAANAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCG
FAPSGWDSLTKYLISKGFEFTELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETL
LYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLIMDDNWYRGELIFVFDG
DAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSSGDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDA
LRRSVPLVARIRDYALRDEYARRLAGWTGWSDEGQVLARVREEANRRSVPERTRRRSVSVEQSPFMQPPGAPADQLAARP
DPRDPTLWPQREALKSVLQYPVLAGPVFDTLTVESFTHPGYGAVRAAIDAAGGASAGITGAQWIDVVRQQTTSALIAGLI
NELGVESIQVDDEKLPRYIDGVLARLQEVWMGRQIAEVKSKLQRMSPIDQGDEYHELFGDLVAMEAYRRSLLEQASGDDM
TA
>Mature_642_residues
MVGRIPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCSEGGDVYAFIQKIEH
VSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAANAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCG
FAPSGWDSLTKYLISKGFEFTELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETL
LYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLIMDDNWYRGELIFVFDG
DAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSSGDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDA
LRRSVPLVARIRDYALRDEYARRLAGWTGWSDEGQVLARVREEANRRSVPERTRRRSVSVEQSPFMQPPGAPADQLAARP
DPRDPTLWPQREALKSVLQYPVLAGPVFDTLTVESFTHPGYGAVRAAIDAAGGASAGITGAQWIDVVRQQTTSALIAGLI
NELGVESIQVDDEKLPRYIDGVLARLQEVWMGRQIAEVKSKLQRMSPIDQGDEYHELFGDLVAMEAYRRSLLEQASGDDM
TA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=517, Percent_Identity=30.9477756286267, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_MYCLE (Q9CCG2)

Other databases:

- EMBL:   AL583919
- PIR:   C87013
- RefSeq:   NP_301629.1
- ProteinModelPortal:   Q9CCG2
- SMR:   Q9CCG2
- EnsemblBacteria:   EBMYCT00000029143
- GeneID:   909792
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0833
- NMPDR:   fig|272631.1.peg.501
- Leproma:   ML0833
- GeneTree:   EBGT00050000015601
- HOGENOM:   HBG573408
- OMA:   HDERSPS
- ProtClustDB:   PRK05667
- BioCyc:   MLEP272631:ML0833-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 70762; Mature: 70762

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRIPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCSEGGDVYAFIQKIEHVSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCCCEEEEH
NAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCGFAPSGWDSLTKYLISKGFEF
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCE
TELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETL
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHH
LYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAML
HEECCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RRLIMDDNWYRGELIFVFDGDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSS
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEEEECCCCCCCEEECC
GDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDALRRSVPLVARIRDYALRDEY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ARRLAGWTGWSDEGQVLARVREEANRRSVPERTRRRSVSVEQSPFMQPPGAPADQLAARP
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC
DPRDPTLWPQREALKSVLQYPVLAGPVFDTLTVESFTHPGYGAVRAAIDAAGGASAGITG
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCH
AQWIDVVRQQTTSALIAGLINELGVESIQVDDEKLPRYIDGVLARLQEVWMGRQIAEVKS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
KLQRMSPIDQGDEYHELFGDLVAMEAYRRSLLEQASGDDMTA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVGRIPDRDIAAIRERVCIEEVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCCCEE
HCFGCSEGGDVYAFIQKIEHVSFVEAVEMLADRIGHTINYFGTATSVQRDRGSRSRLIAA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCCCEEEEH
NAAAAAFYAAALESDEAAPARQYLMERNFDANAARHFGCGFAPSGWDSLTKYLISKGFEF
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCE
TELEAAGLSRQGRRGPMDRFHRRLLWPILTLAGEVIGFGARRLFDDDPMEAKYVNTPETL
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHH
LYKKSSVMFGIDLAKRDIVKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAML
HEECCCEEEEHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RRLIMDDNWYRGELIFVFDGDAAGQAAALKAFDGDQQVAGKSFVAVAADGMDPCDLRLSS
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHCCCCEEEEEECCCCCCCEEECC
GDQALRDLVACRKPMFEFAIRSLIPDGMVLDDDPQAKVDALRRSVPLVARIRDYALRDEY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ARRLAGWTGWSDEGQVLARVREEANRRSVPERTRRRSVSVEQSPFMQPPGAPADQLAARP
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHCCCC
DPRDPTLWPQREALKSVLQYPVLAGPVFDTLTVESFTHPGYGAVRAAIDAAGGASAGITG
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCH
AQWIDVVRQQTTSALIAGLINELGVESIQVDDEKLPRYIDGVLARLQEVWMGRQIAEVKS
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
KLQRMSPIDQGDEYHELFGDLVAMEAYRRSLLEQASGDDMTA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002