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The map label for this gene is aroA

Identifier: 221229822

GI number: 221229822

Start: 935517

End: 936809

Strand: Reverse

Name: aroA

Synonym: MLBr_00792

Alternate gene names: 221229822

Gene position: 936809-935517 (Counterclockwise)

Preceding gene: 221229825

Following gene: 221229821

Centisome position: 28.67

GC content: 62.57

Gene sequence:

>1293_bases
GTGAGCATCGAGTCCTGGACGGCCCCCGTCGCGTCGACACCGGTGCAGGCGAACGTGACAATTCCGGGCTCTAAATCGCA
AACCAACCGAGCACTGATGCTGGCAGCGCTAGCGGCAGCACAAGGCCAAGGCACCTCGACCATCGGCGGTGCACTGCGCA
GCAGGGATACCGAACTAATGATTTCAGCGTTACGGACACTGGGCCTACACGTCGACGAGGCCGGCTCTGTCTTAACGGTC
AACGGCCGAATTACCCCCGGCGAAGACGCTCAAGTGGACTGCGGTCTGGCCGGCACAGTATTGCGTTTTGTCCCACCGCT
GGCGGCGCTGAGCGCCAACCCGGTCACCTTCGACGGCGACGAACAAGCCCGGGCCCGGCCGATCACACCGCTGCTGGATG
CGCTGCGTGGTCTGGGCGTCCCCGTCGATGGCGTCGGTTTGCCTTTCCAGGTGCAAGGCAGCGGGTCAGTCGCCGGTGGC
ACCGTGGCCATTGACGCGTCGGCGTCCTCACAGTTCGTCTCCGGCCTGTTGTTGTGCGCAGCGTCGTTCACCCAAGGAGT
GACCGTGCAGCACACCGGTTCGCCGGTACCGTCAGCTCCGCATATAGCGATGACGGTTATGATGCTGCGGCAAGCCGGTG
TCCAGGTCGATGACTCGGTTGGTAACCGTTGGCAGGTGCGACCCGGTACAGTTGCGGCCCGGCACTGGGTCGTCGAGCCC
GATCTCACGAACGCTGTTGCGTTCCTGGCGGCAGCAGCGGTCAGCGGCGGTACCGTGCGCATCACGGGCTGGCCAAAAAC
CAGTGTACAGCCCGCAGACAATATTCTGAACATCTTGTTCCGACTGAATGTCGTTGTTAATCAAACTGATTCATTCCTCG
AAGTACAAGGATCGACGGTGTACGATGGCTTCGACGTCGACCTTCGCGACGTCGGTGAGCTCACGCCGTCGGTAGCAGCG
CTAGCGGCACTGGCAACCCCAGGATCGGTGTCGCAACTACACGGCATCGCCCATCTGCGCGGCCACGAAACCGACCGACT
CGCCGCACTGAGCACCGAAATCAACCGTCTGGGCGGCGACTGTCAGGAAACGTCCGACGGTTTGATTATCACCGCGACAC
CGCTGCGGCCCGGCGTCTGGCGAGCGTATGCTGATCACAGGATGGCAATGGCCGGCGCGATCGTCGGGTTGCGGGTCTCC
GGAGTCGAAGTCGACGACATCGGTGCTACCCACAAGACGCTACCCCAGTTCCCACAACTGTGGGCGAACATGCTCAAGAG
GTCGACCGGTTGA

Upstream 100 bases:

>100_bases
AGCTAATAGAGCCGTACTGGTAATGACCGCAAACCGTCCGCACACGCCTGCCATGATGCCCGATACCCATAACGCCCGCC
AACACGGCAGGATGTAGCAG

Downstream 100 bases:

>100_bases
AGCCCGACAACTACTACGACGAATCCGATGTCAAGATTCGCTCCGGCCGAGGCTCACGACCGCGGACCAAAACCCGTCCC
GAACACGCTGACGCCAAGGC

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSIESWTAPVASTPVQANVTIPGSKSQTNRALMLAALAAAQGQGTSTIGGALRSRDTELMISALRTLGLHVDEAGSVLTV
NGRITPGEDAQVDCGLAGTVLRFVPPLAALSANPVTFDGDEQARARPITPLLDALRGLGVPVDGVGLPFQVQGSGSVAGG
TVAIDASASSQFVSGLLLCAASFTQGVTVQHTGSPVPSAPHIAMTVMMLRQAGVQVDDSVGNRWQVRPGTVAARHWVVEP
DLTNAVAFLAAAAVSGGTVRITGWPKTSVQPADNILNILFRLNVVVNQTDSFLEVQGSTVYDGFDVDLRDVGELTPSVAA
LAALATPGSVSQLHGIAHLRGHETDRLAALSTEINRLGGDCQETSDGLIITATPLRPGVWRAYADHRMAMAGAIVGLRVS
GVEVDDIGATHKTLPQFPQLWANMLKRSTG

Sequences:

>Translated_430_residues
MSIESWTAPVASTPVQANVTIPGSKSQTNRALMLAALAAAQGQGTSTIGGALRSRDTELMISALRTLGLHVDEAGSVLTV
NGRITPGEDAQVDCGLAGTVLRFVPPLAALSANPVTFDGDEQARARPITPLLDALRGLGVPVDGVGLPFQVQGSGSVAGG
TVAIDASASSQFVSGLLLCAASFTQGVTVQHTGSPVPSAPHIAMTVMMLRQAGVQVDDSVGNRWQVRPGTVAARHWVVEP
DLTNAVAFLAAAAVSGGTVRITGWPKTSVQPADNILNILFRLNVVVNQTDSFLEVQGSTVYDGFDVDLRDVGELTPSVAA
LAALATPGSVSQLHGIAHLRGHETDRLAALSTEINRLGGDCQETSDGLIITATPLRPGVWRAYADHRMAMAGAIVGLRVS
GVEVDDIGATHKTLPQFPQLWANMLKRSTG
>Mature_429_residues
SIESWTAPVASTPVQANVTIPGSKSQTNRALMLAALAAAQGQGTSTIGGALRSRDTELMISALRTLGLHVDEAGSVLTVN
GRITPGEDAQVDCGLAGTVLRFVPPLAALSANPVTFDGDEQARARPITPLLDALRGLGVPVDGVGLPFQVQGSGSVAGGT
VAIDASASSQFVSGLLLCAASFTQGVTVQHTGSPVPSAPHIAMTVMMLRQAGVQVDDSVGNRWQVRPGTVAARHWVVEPD
LTNAVAFLAAAAVSGGTVRITGWPKTSVQPADNILNILFRLNVVVNQTDSFLEVQGSTVYDGFDVDLRDVGELTPSVAAL
AALATPGSVSQLHGIAHLRGHETDRLAALSTEINRLGGDCQETSDGLIITATPLRPGVWRAYADHRMAMAGAIVGLRVSG
VEVDDIGATHKTLPQFPQLWANMLKRSTG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=443, Percent_Identity=31.6027088036117, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320332, Length=468, Percent_Identity=28.6324786324786, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_MYCLE (Q9CCI3)

Other databases:

- EMBL:   AL583919
- PIR:   A87008
- RefSeq:   NP_301607.1
- ProteinModelPortal:   Q9CCI3
- SMR:   Q9CCI3
- EnsemblBacteria:   EBMYCT00000028666
- GeneID:   909741
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0792
- NMPDR:   fig|272631.1.peg.479
- Leproma:   ML0792
- GeneTree:   EBGT00050000016231
- HOGENOM:   HBG646626
- OMA:   HRMATAG
- ProtClustDB:   PRK02427
- BioCyc:   MLEP272631:ML0792-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 44558; Mature: 44427

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIESWTAPVASTPVQANVTIPGSKSQTNRALMLAALAAAQGQGTSTIGGALRSRDTELM
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHH
ISALRTLGLHVDEAGSVLTVNGRITPGEDAQVDCGLAGTVLRFVPPLAALSANPVTFDGD
HHHHHHHCCEEECCCCEEEECCEECCCCCCEECCCHHHHHHHHHCCHHHHCCCCEEECCC
EQARARPITPLLDALRGLGVPVDGVGLPFQVQGSGSVAGGTVAIDASASSQFVSGLLLCA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHH
ASFTQGVTVQHTGSPVPSAPHIAMTVMMLRQAGVQVDDSVGNRWQVRPGTVAARHWVVEP
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEECCCCEEEEEEEECC
DLTNAVAFLAAAAVSGGTVRITGWPKTSVQPADNILNILFRLNVVVNQTDSFLEVQGSTV
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCEE
YDGFDVDLRDVGELTPSVAALAALATPGSVSQLHGIAHLRGHETDRLAALSTEINRLGGD
ECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
CQETSDGLIITATPLRPGVWRAYADHRMAMAGAIVGLRVSGVEVDDIGATHKTLPQFPQL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECEEECCCCCCHHHHHHHHHH
WANMLKRSTG
HHHHHHHCCC
>Mature Secondary Structure 
SIESWTAPVASTPVQANVTIPGSKSQTNRALMLAALAAAQGQGTSTIGGALRSRDTELM
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHH
ISALRTLGLHVDEAGSVLTVNGRITPGEDAQVDCGLAGTVLRFVPPLAALSANPVTFDGD
HHHHHHHCCEEECCCCEEEECCEECCCCCCEECCCHHHHHHHHHCCHHHHCCCCEEECCC
EQARARPITPLLDALRGLGVPVDGVGLPFQVQGSGSVAGGTVAIDASASSQFVSGLLLCA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHH
ASFTQGVTVQHTGSPVPSAPHIAMTVMMLRQAGVQVDDSVGNRWQVRPGTVAARHWVVEP
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEECCCCEEEEEEEECC
DLTNAVAFLAAAAVSGGTVRITGWPKTSVQPADNILNILFRLNVVVNQTDSFLEVQGSTV
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCEE
YDGFDVDLRDVGELTPSVAALAALATPGSVSQLHGIAHLRGHETDRLAALSTEINRLGGD
ECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
CQETSDGLIITATPLRPGVWRAYADHRMAMAGAIVGLRVSGVEVDDIGATHKTLPQFPQL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECEEECCCCCCHHHHHHHHHH
WANMLKRSTG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002