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The map label for this gene is fadE25 [H]

Identifier: 221229792

GI number: 221229792

Start: 877862

End: 879031

Strand: Direct

Name: fadE25 [H]

Synonym: MLBr_00737

Alternate gene names: 221229792

Gene position: 877862-879031 (Clockwise)

Preceding gene: 221229791

Following gene: 221229796

Centisome position: 26.86

GC content: 57.35

Gene sequence:

>1170_bases
ATGGTTGGGTGGTCCGGAAACCCATTGTTTGATCTATTCAAGCTGCCAGAAGAACACAACGAATTGCGGGCAACCATCCG
CGCGTTGGCGGAAAAAGAGATCGCTCCGCATGCCGCTGATGTGGACCAGCGTGCTCGATTTCCCGAGGAAGCGCTGGCAG
CCCTGAATGCATCAGGTTTCAACGCTATCCACGTTCCCGAGGAGTATGGTGGTCAGGGTGCGGATTCGGTAGCGGCTTGC
ATTGTGATCGAAGAAGTGGCGCGTGTCGATGCTTCTGCATCGTTGATTCCTGCAGTTAACAAGCTTGGCACCATGGGACT
CATCCTGCGCGGTTCGGAAGAGCTCAAGAAACAGGTTCTGCCATCGTTGGCTGCGGAGGGGGCGATGGCGTCCTATGCAT
TAAGTGAGCGCGAAGCCGGCAGTGACGCTGCGTCGATGCGGACCCGGGCCAAAGCTGACGGGGATGACTGGATTCTCAAT
GGCTTCAAGTGCTGGATTACCAACGGTGGCAAGTCGACCTGGTACACGGTTATGGCGGTGACCGATCCGGACAAGGGCGC
CAACGGCATCTCGGCGTTCATCGTGCACAAGGACGATGAGGGATTCAGCATTGGCCCGAAAGAAAAGAAGCTCGGGATCA
AGGGGTCACCAACCACCGAACTCTACTTCGATAAATGTCGCATCCCCGGTGATCGCATCATTGGTGAGCCCGGTACTGGC
TTTAAGACAGCGCTAGCCACGTTGGATCACACGCGTCCCACGATTGGTGCCCAAGCCGTGGGCATTGCGCAGGGCGCGTT
GGACGCTGCCATCGTTTATACCAAGGACCGCAAGCAATTCGGCGAGTCGATTAGCACTTTCCAGTCCATTCAGTTCATGC
TCGCCGACATGGCGATGAAAGTGGAGGCTGCACGGTTAATTGTCTACGCTGCCGCTGCCCGTGCTGAACGCGGTGAGCCG
GATCTGGGCTTTATTTCAGCGGCGTCGAAATGCTTTGCTTCCGACATTGCGATGGAGGTCACCACCGACGCTGTGCAATT
GTTTGGCGGCGCGGGCTACACTTCCGACTTCCCCGTCGAGCGGTTCATGCGCGACGCCAAGATCACACAGATCTATGAGG
GGACCAATCAGATTCAGCGTGTGGTGATGTCGCGGGCGCTGCTGCGCTGA

Upstream 100 bases:

>100_bases
GACTAATGTCTGACTCTCTCCCCGCACGCGGGTGGGGATGGTCCCACCGCAGACCGCAAGTAATCCGTCCGCATCATGGT
CAGGTTAGAAGGAGGCAGTG

Downstream 100 bases:

>100_bases
TCCGGGGTGCGTTGGGCCGGCGAAATCGCTTGCTCGACAAGCAGTCACCACTAGGGCGAACATGTGCAACCCTGTGGCGT
CACAACCGGGGTTACCCATC

Product: putative acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIAPHAADVDQRARFPEEALAALNASGFNAIHVPEEYGGQGADSVAAC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPSLAAEGAMASYALSEREAGSDAASMRTRAKADGDDWILN
GFKCWITNGGKSTWYTVMAVTDPDKGANGISAFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQFMLADMAMKVEAARLIVYAAAARAERGEP
DLGFISAASKCFASDIAMEVTTDAVQLFGGAGYTSDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR

Sequences:

>Translated_389_residues
MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIAPHAADVDQRARFPEEALAALNASGFNAIHVPEEYGGQGADSVAAC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPSLAAEGAMASYALSEREAGSDAASMRTRAKADGDDWILN
GFKCWITNGGKSTWYTVMAVTDPDKGANGISAFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQFMLADMAMKVEAARLIVYAAAARAERGEP
DLGFISAASKCFASDIAMEVTTDAVQLFGGAGYTSDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR
>Mature_389_residues
MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIAPHAADVDQRARFPEEALAALNASGFNAIHVPEEYGGQGADSVAAC
IVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVLPSLAAEGAMASYALSEREAGSDAASMRTRAKADGDDWILN
GFKCWITNGGKSTWYTVMAVTDPDKGANGISAFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTG
FKTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQFMLADMAMKVEAARLIVYAAAARAERGEP
DLGFISAASKCFASDIAMEVTTDAVQLFGGAGYTSDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=386, Percent_Identity=44.559585492228, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI4557231, Length=376, Percent_Identity=41.7553191489362, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI187960098, Length=376, Percent_Identity=41.7553191489362, Blast_Score=297, Evalue=9e-81,
Organism=Homo sapiens, GI4501859, Length=374, Percent_Identity=39.0374331550802, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI21361497, Length=389, Percent_Identity=36.7609254498715, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI7656849, Length=379, Percent_Identity=35.0923482849604, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI226958412, Length=383, Percent_Identity=35.7702349869452, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI226958414, Length=354, Percent_Identity=36.1581920903955, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI76496475, Length=344, Percent_Identity=37.2093023255814, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI4557235, Length=344, Percent_Identity=37.2093023255814, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4501857, Length=373, Percent_Identity=31.3672922252011, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4503943, Length=387, Percent_Identity=25.0645994832041, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI7669494, Length=373, Percent_Identity=24.6648793565684, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI211971008, Length=295, Percent_Identity=26.7796610169492, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI48976061, Length=295, Percent_Identity=26.7796610169492, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786223, Length=381, Percent_Identity=29.9212598425197, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI87081958, Length=380, Percent_Identity=31.5789473684211, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87081702, Length=379, Percent_Identity=27.7044854881267, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082384, Length=319, Percent_Identity=22.884012539185, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17570075, Length=379, Percent_Identity=41.688654353562, Blast_Score=307, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI17508101, Length=371, Percent_Identity=41.5094339622642, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17569725, Length=379, Percent_Identity=39.3139841688654, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17534899, Length=380, Percent_Identity=38.6842105263158, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17506239, Length=364, Percent_Identity=39.010989010989, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17533517, Length=361, Percent_Identity=38.2271468144044, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17538396, Length=388, Percent_Identity=37.3711340206186, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71990804, Length=295, Percent_Identity=42.7118644067797, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71985184, Length=383, Percent_Identity=34.7258485639687, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI32563615, Length=229, Percent_Identity=42.7947598253275, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71982178, Length=280, Percent_Identity=34.2857142857143, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71985192, Length=317, Percent_Identity=35.0157728706625, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17505929, Length=378, Percent_Identity=29.6296296296296, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17551932, Length=374, Percent_Identity=29.4117647058824, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17531909, Length=236, Percent_Identity=34.3220338983051, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17534353, Length=378, Percent_Identity=25.1322751322751, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531913, Length=188, Percent_Identity=30.3191489361702, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI86563381, Length=323, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI86563383, Length=323, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562446, Length=342, Percent_Identity=26.6081871345029, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660351, Length=378, Percent_Identity=39.9470899470899, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI21356377, Length=379, Percent_Identity=40.8970976253298, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24646207, Length=378, Percent_Identity=40.2116402116402, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24666513, Length=374, Percent_Identity=37.9679144385027, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI21355753, Length=390, Percent_Identity=35.6410256410256, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI281363737, Length=355, Percent_Identity=36.6197183098592, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI19920834, Length=393, Percent_Identity=28.7531806615776, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221473076, Length=324, Percent_Identity=26.2345679012346, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550141, Length=324, Percent_Identity=26.2345679012346, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 41720; Mature: 41720

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIAPHAADVDQRARFPEEALAALNASGF
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCC
NAIHVPEEYGGQGADSVAACIVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVL
CEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHH
PSLAAEGAMASYALSEREAGSDAASMRTRAKADGDDWILNGFKCWITNGGKSTWYTVMAV
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCEEEEEECCCCCEEEEEEEE
TDPDKGANGISAFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTG
ECCCCCCCCEEEEEEEECCCCCCCCCCHHCCCCCCCCCCCEEEHHCCCCCCCEECCCCCC
FKTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQFMLADMAMK
HHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VEAARLIVYAAAARAERGEPDLGFISAASKCFASDIAMEVTTDAVQLFGGAGYTSDFPVE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RFMRDAKITQIYEGTNQIQRVVMSRALLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVGWSGNPLFDLFKLPEEHNELRATIRALAEKEIAPHAADVDQRARFPEEALAALNASGF
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCCCCC
NAIHVPEEYGGQGADSVAACIVIEEVARVDASASLIPAVNKLGTMGLILRGSEELKKQVL
CEEECCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHH
PSLAAEGAMASYALSEREAGSDAASMRTRAKADGDDWILNGFKCWITNGGKSTWYTVMAV
HHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCEEEEEECCCCCEEEEEEEE
TDPDKGANGISAFIVHKDDEGFSIGPKEKKLGIKGSPTTELYFDKCRIPGDRIIGEPGTG
ECCCCCCCCEEEEEEEECCCCCCCCCCHHCCCCCCCCCCCEEEHHCCCCCCCEECCCCCC
FKTALATLDHTRPTIGAQAVGIAQGALDAAIVYTKDRKQFGESISTFQSIQFMLADMAMK
HHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VEAARLIVYAAAARAERGEPDLGFISAASKCFASDIAMEVTTDAVQLFGGAGYTSDFPVE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
RFMRDAKITQIYEGTNQIQRVVMSRALLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]