Click here to switch to the map view.

The map label for this gene is purK

Identifier: 221229790

GI number: 221229790

Start: 875933

End: 877252

Strand: Direct

Name: purK

Synonym: MLBr_00735

Alternate gene names: 221229790

Gene position: 875933-877252 (Clockwise)

Preceding gene: 221229788

Following gene: 221229791

Centisome position: 26.8

GC content: 62.73

Gene sequence:

>1320_bases
ATGATGGCTGTGCCGAGTAGATGCTCGCTTGGAGTTGCTCCATTGGTTGCCATGGTCGGTGGCGGTCAACTGGCTCGGAT
GACCCATCAAGCCGCGATCGCGCTGGGGCAGACCCTGCGGGTGTTAGCTACCGCTGCAGACGAACCCGCTGCTCAGGTCA
CCCCCGATGTGGTGATCGGCTCGCACACTGATCTTGAAGACCTGCGCCGCGTTGCCCTCGGAGCCGATGCGTTGACGTTC
GATCACGAACACGTCCCGACTGAACTGCTGGACAAACTGGTGGCCGAAGGCATCAATGTGGCACCGTCGCCGCAAGCTCT
GGTGCACGCCCAGGACAAGCTGGTCATGCGGCGGCGATTGGCGGCGCTGGGAGCTGCGATGCCTCGCTTCATGGCATTAG
ACAGTGTGGACGACTTGGCCGAAATCGATGCGTTCGCGCAGCGCCTTACGGGTTCGAAAGATGCCCCGATGGTGGTCAAG
GCGGTCCGCGGGGGTTATGACGGCCGGGGGGTGCAGATGGTCCGCGACTCGGCACACGCCCGCGAGGTCGCCTCCGGATA
TCTAGTCGACGGAATGCCGGTCCTGGTTGAGGAGCGAGTGGAACTGCGTCGCGAATTGTCCGCGCTGGTAGCGCGCTCGC
CGTTCGGCCAGGGTGCAGCCTGGCCAGTCGTGGAGACGGTGCAACGGGACGGGATTTGCGTTTTGGTGGTCGCACCGGCG
TTGGCGTTGGCCGACGACCTGGCCTCGGCTGCGCAGCAGCTGGCGTTGCGATTGGCCGCTGAACTCGGCGTCGTCGGTGT
GTTCGCGGTCGAGTTGTTCGAAACCGCCGACGGTGCTTTGCTGGTGAACGAGCTCGCAATGCGGCCGCACAACTCCGGAC
ACTGGACAATGGACGGTGCTCGCACCAGCCAGTTCGAGCAGCATCTCCGTGCGGTGCTAGACTATCCCCTCGGCGAGACC
GACGCCGTCGCGCCGGTGACCGTGATGGTTAACGTGCTAGGCGCCCCACAACCGCCGACGCTGAGTGTAGTGACTATGGA
TGAACGGCTACACCACCTGTTTGCGCGGATGCCCGATGCTCGGGTTCATCTTTACGACAAGGTTGAGCGTCCTGGTCGCA
AGGTTGGGCACATCAATTTTCGCGGCACCGATAAAGACCGAAAAAACCCCACTAAGCTGCGTGAACGCGCTGAGTTGGCA
GCGCATTGGCTATCACACGGGCAGTGGACGGATGGATGGGATCCACACCGTGCCGGCGACGATGTAGTGGAGATATCTCT
CGCTTGCGGAGGACGAAACGATGCGCAGAGGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGGGAAAAGACACAACACGTCAGTGTACGGATTGACGTAAAATGCTACCTAACGGTTGGGTATTACTACGGGTCAATATG
CTCTCTGTCGCCATGACACC

Downstream 100 bases:

>100_bases
TGACCAGGCAGCCCCGAGTCGGGGTGATCATGGGCAGCGACAGCGACTGGTCGGTGATGCAAGATGCGGCCCATGCACTG
GCGGAGTTTGACATCCCGAT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MMAVPSRCSLGVAPLVAMVGGGQLARMTHQAAIALGQTLRVLATAADEPAAQVTPDVVIGSHTDLEDLRRVALGADALTF
DHEHVPTELLDKLVAEGINVAPSPQALVHAQDKLVMRRRLAALGAAMPRFMALDSVDDLAEIDAFAQRLTGSKDAPMVVK
AVRGGYDGRGVQMVRDSAHAREVASGYLVDGMPVLVEERVELRRELSALVARSPFGQGAAWPVVETVQRDGICVLVVAPA
LALADDLASAAQQLALRLAAELGVVGVFAVELFETADGALLVNELAMRPHNSGHWTMDGARTSQFEQHLRAVLDYPLGET
DAVAPVTVMVNVLGAPQPPTLSVVTMDERLHHLFARMPDARVHLYDKVERPGRKVGHINFRGTDKDRKNPTKLRERAELA
AHWLSHGQWTDGWDPHRAGDDVVEISLACGGRNDAQRQR

Sequences:

>Translated_439_residues
MMAVPSRCSLGVAPLVAMVGGGQLARMTHQAAIALGQTLRVLATAADEPAAQVTPDVVIGSHTDLEDLRRVALGADALTF
DHEHVPTELLDKLVAEGINVAPSPQALVHAQDKLVMRRRLAALGAAMPRFMALDSVDDLAEIDAFAQRLTGSKDAPMVVK
AVRGGYDGRGVQMVRDSAHAREVASGYLVDGMPVLVEERVELRRELSALVARSPFGQGAAWPVVETVQRDGICVLVVAPA
LALADDLASAAQQLALRLAAELGVVGVFAVELFETADGALLVNELAMRPHNSGHWTMDGARTSQFEQHLRAVLDYPLGET
DAVAPVTVMVNVLGAPQPPTLSVVTMDERLHHLFARMPDARVHLYDKVERPGRKVGHINFRGTDKDRKNPTKLRERAELA
AHWLSHGQWTDGWDPHRAGDDVVEISLACGGRNDAQRQR
>Mature_439_residues
MMAVPSRCSLGVAPLVAMVGGGQLARMTHQAAIALGQTLRVLATAADEPAAQVTPDVVIGSHTDLEDLRRVALGADALTF
DHEHVPTELLDKLVAEGINVAPSPQALVHAQDKLVMRRRLAALGAAMPRFMALDSVDDLAEIDAFAQRLTGSKDAPMVVK
AVRGGYDGRGVQMVRDSAHAREVASGYLVDGMPVLVEERVELRRELSALVARSPFGQGAAWPVVETVQRDGICVLVVAPA
LALADDLASAAQQLALRLAAELGVVGVFAVELFETADGALLVNELAMRPHNSGHWTMDGARTSQFEQHLRAVLDYPLGET
DAVAPVTVMVNVLGAPQPPTLSVVTMDERLHHLFARMPDARVHLYDKVERPGRKVGHINFRGTDKDRKNPTKLRERAELA
AHWLSHGQWTDGWDPHRAGDDVVEISLACGGRNDAQRQR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=234, Percent_Identity=39.7435897435897, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788155, Length=380, Percent_Identity=28.6842105263158, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=373, Percent_Identity=33.5120643431635, Blast_Score=189, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_MYCLE (P46701)

Other databases:

- EMBL:   U00012
- EMBL:   AL583919
- PIR:   H87000
- RefSeq:   NP_301575.1
- ProteinModelPortal:   P46701
- SMR:   P46701
- EnsemblBacteria:   EBMYCT00000028675
- GeneID:   909683
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0735
- NMPDR:   fig|272631.1.peg.447
- Leproma:   ML0735
- GeneTree:   EBGT00050000015722
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- ProtClustDB:   PRK06019
- BioCyc:   MLEP272631:ML0735-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 47307; Mature: 47307

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 113-113 BINDING 160-160 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAVPSRCSLGVAPLVAMVGGGQLARMTHQAAIALGQTLRVLATAADEPAAQVTPDVVIG
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
SHTDLEDLRRVALGADALTFDHEHVPTELLDKLVAEGINVAPSPQALVHAQDKLVMRRRL
CCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AALGAAMPRFMALDSVDDLAEIDAFAQRLTGSKDAPMVVKAVRGGYDGRGVQMVRDSAHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
REVASGYLVDGMPVLVEERVELRRELSALVARSPFGQGAAWPVVETVQRDGICVLVVAPA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEEEHHHH
LALADDLASAAQQLALRLAAELGVVGVFAVELFETADGALLVNELAMRPHNSGHWTMDGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCC
RTSQFEQHLRAVLDYPLGETDAVAPVTVMVNVLGAPQPPTLSVVTMDERLHHLFARMPDA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCC
RVHLYDKVERPGRKVGHINFRGTDKDRKNPTKLRERAELAAHWLSHGQWTDGWDPHRAGD
HHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DVVEISLACGGRNDAQRQR
CEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MMAVPSRCSLGVAPLVAMVGGGQLARMTHQAAIALGQTLRVLATAADEPAAQVTPDVVIG
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
SHTDLEDLRRVALGADALTFDHEHVPTELLDKLVAEGINVAPSPQALVHAQDKLVMRRRL
CCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AALGAAMPRFMALDSVDDLAEIDAFAQRLTGSKDAPMVVKAVRGGYDGRGVQMVRDSAHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
REVASGYLVDGMPVLVEERVELRRELSALVARSPFGQGAAWPVVETVQRDGICVLVVAPA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEEEHHHH
LALADDLASAAQQLALRLAAELGVVGVFAVELFETADGALLVNELAMRPHNSGHWTMDGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEECCC
RTSQFEQHLRAVLDYPLGETDAVAPVTVMVNVLGAPQPPTLSVVTMDERLHHLFARMPDA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCC
RVHLYDKVERPGRKVGHINFRGTDKDRKNPTKLRERAELAAHWLSHGQWTDGWDPHRAGD
HHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DVVEISLACGGRNDAQRQR
CEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7489918; 11234002