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The map label for this gene is sseA

Identifier: 221229783

GI number: 221229783

Start: 870037

End: 870927

Strand: Reverse

Name: sseA

Synonym: MLBr_00728

Alternate gene names: 221229783

Gene position: 870927-870037 (Counterclockwise)

Preceding gene: 221229784

Following gene: 221229782

Centisome position: 26.65

GC content: 59.48

Gene sequence:

>891_bases
GTGCCGCTACCCACAGATCCAAGCCCTTCCCTGTCGGCTTACGCCCACCCCGAACGGCTAGTAACCGGTGATTGGCTGTA
CTTCCATCTGGGCAAACCCGGTCTGGCTATAGTCGAATCCGACGAGAACGTACTGCTCTACGATGTCGGACATATTCCTG
GCGCGGTGAAGGTCGACTGGCACACCGACCTCAATGACCCGAAGGTGCGTGACTACATCACTGGCGAGCAATTCGCCGAC
TTGATGAACCGCAAGGGCATCGCCCGCGACGACACCGTGGTGATCTACGGCGACAAGAGCAACTGGTGGGCGGCCTACGC
ACTGTGGGTCTTTACCTTGTTCGGCCATCCCGACGTGCGACTGCTCAACGGCGGTCGTGATCTATGGCTCGCCGAACGCC
GGGATACCAGCCTGGCCGTGCCGAATAAGACATCGACCAGCTATCCCGTGGTAAACCGGAACGACGCACCCATCCGCGCA
TTCAAAGACGACGTGTTGGCCATCCTCGGCACTCAGCCGCTGATCGACGTGCGATCCCTCGACGAGTACACCGGCAAATG
CACCGAAATGCCCGACTCCCCCGAAGAAAGTGTGCTGCGAGCCGGCCACATCCCCACCGCCAGGTCGATCCCGTGGGAAA
TGACAGTCGACAAAAGCGGTCGATTCCGCAGCAGCGAAGAATTGGAACGGCTCTATGACTTCATCACCCCAAACGATAAA
ACCATCGTATATTGCCGCATCGGCGAGCGATCCAGCCACACTTGGTTCGTACTCACCCATCTGCTGGGCAAACCGGGAGT
GCGTAACTATGACGGCTCGTGGACCGAGTGGGGGAACACCGTACGAGTGCCGATCACTGCAGGCGAAAGCCCCGGAGCCG
TACCTGTCTGA

Upstream 100 bases:

>100_bases
GGACTACCCGAGATACCAGACTGACATAGCCCAATGGACATCAGCGCTTCAGACTCTAAGCATGATCACCAACAACATCA
GGATGAGTAAGCTTTAAGGT

Downstream 100 bases:

>100_bases
TAGCCATTCCGTGAGAATACCCGCGCCCTCGGCCGTCCTGGCCTCAGACTTCGACGAAGTCTGAGGCCAGGACGAGCTAA
AACTGTTGCTGGAATTTGCC

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPLPTDPSPSLSAYAHPERLVTGDWLYFHLGKPGLAIVESDENVLLYDVGHIPGAVKVDWHTDLNDPKVRDYITGEQFAD
LMNRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLNGGRDLWLAERRDTSLAVPNKTSTSYPVVNRNDAPIRA
FKDDVLAILGTQPLIDVRSLDEYTGKCTEMPDSPEESVLRAGHIPTARSIPWEMTVDKSGRFRSSEELERLYDFITPNDK
TIVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTEWGNTVRVPITAGESPGAVPV

Sequences:

>Translated_296_residues
MPLPTDPSPSLSAYAHPERLVTGDWLYFHLGKPGLAIVESDENVLLYDVGHIPGAVKVDWHTDLNDPKVRDYITGEQFAD
LMNRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLNGGRDLWLAERRDTSLAVPNKTSTSYPVVNRNDAPIRA
FKDDVLAILGTQPLIDVRSLDEYTGKCTEMPDSPEESVLRAGHIPTARSIPWEMTVDKSGRFRSSEELERLYDFITPNDK
TIVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTEWGNTVRVPITAGESPGAVPV
>Mature_295_residues
PLPTDPSPSLSAYAHPERLVTGDWLYFHLGKPGLAIVESDENVLLYDVGHIPGAVKVDWHTDLNDPKVRDYITGEQFADL
MNRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHPDVRLLNGGRDLWLAERRDTSLAVPNKTSTSYPVVNRNDAPIRAF
KDDVLAILGTQPLIDVRSLDEYTGKCTEMPDSPEESVLRAGHIPTARSIPWEMTVDKSGRFRSSEELERLYDFITPNDKT
IVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTEWGNTVRVPITAGESPGAVPV

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI194473668, Length=300, Percent_Identity=28.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI17402865, Length=279, Percent_Identity=29.7491039426523, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI61835204, Length=246, Percent_Identity=28.8617886178862, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI194473681, Length=246, Percent_Identity=28.8617886178862, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87082121, Length=282, Percent_Identity=29.0780141843972, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081967, Length=303, Percent_Identity=27.0627062706271, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=300, Percent_Identity=27.6666666666667, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THT2_MYCLE (P46700)

Other databases:

- EMBL:   U00012
- EMBL:   AL583919
- PIR:   A87000
- RefSeq:   NP_301568.1
- ProteinModelPortal:   P46700
- SMR:   P46700
- EnsemblBacteria:   EBMYCT00000028436
- GeneID:   909669
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0728
- NMPDR:   fig|272631.1.peg.440
- Leproma:   ML0728
- GeneTree:   EBGT00050000015573
- HOGENOM:   HBG709927
- OMA:   EERDTSY
- ProtClustDB:   CLSK2460474
- BioCyc:   MLEP272631:ML0728-MONOMER
- BRENDA:   2.8.1.1
- InterPro:   IPR001763
- InterPro:   IPR001307
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 33236; Mature: 33105

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 245-245 BINDING 250-250

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPTDPSPSLSAYAHPERLVTGDWLYFHLGKPGLAIVESDENVLLYDVGHIPGAVKVDW
CCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEE
HTDLNDPKVRDYITGEQFADLMNRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHPDVR
ECCCCCCHHHHEECHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
LLNGGRDLWLAERRDTSLAVPNKTSTSYPVVNRNDAPIRAFKDDVLAILGTQPLIDVRSL
EEECCCEEEEEECCCCEEECCCCCCCCCCEEECCCCCCHHHHHCEEEEECCCCCEEHHHH
DEYTGKCTEMPDSPEESVLRAGHIPTARSIPWEMTVDKSGRFRSSEELERLYDFITPNDK
HHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCC
TIVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTEWGNTVRVPITAGESPGAVPV
EEEEEEECCCCCCEEEEEEHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PLPTDPSPSLSAYAHPERLVTGDWLYFHLGKPGLAIVESDENVLLYDVGHIPGAVKVDW
CCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEE
HTDLNDPKVRDYITGEQFADLMNRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHPDVR
ECCCCCCHHHHEECHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
LLNGGRDLWLAERRDTSLAVPNKTSTSYPVVNRNDAPIRAFKDDVLAILGTQPLIDVRSL
EEECCCEEEEEECCCCEEECCCCCCCCCCEEECCCCCCHHHHHCEEEEECCCCCEEHHHH
DEYTGKCTEMPDSPEESVLRAGHIPTARSIPWEMTVDKSGRFRSSEELERLYDFITPNDK
HHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCC
TIVYCRIGERSSHTWFVLTHLLGKPGVRNYDGSWTEWGNTVRVPITAGESPGAVPV
EEEEEEECCCCCCEEEEEEHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7489918; 11234002