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The map label for this gene is trpS

Identifier: 221229767

GI number: 221229767

Start: 822135

End: 823166

Strand: Direct

Name: trpS

Synonym: MLBr_00686

Alternate gene names: 221229767

Gene position: 822135-823166 (Clockwise)

Preceding gene: 221229764

Following gene: 221229768

Centisome position: 25.16

GC content: 59.01

Gene sequence:

>1032_bases
ATGAGTACCGCTACCGGATTCAGCAGGATTTTCTCCGGCGTGCAGCCCACTTCTGATTCGCTTCACCTCGGTAACGCGTT
GGGTGCCATCACCCAGTGGGTTGCGCTGCAGTACGATCATCCGGACGAATACGAAGCGTTCTTTTGTGTGGTTGACCTGC
ACGCCATAACCATCGCGCAAGATCCCGAGACACTGTGGCGTCGGACCCTGGTCACTGCCGCGCAATACCTGGCCTTGGGC
ATCGATCCGGGGCGCGCCGTCGTTTTCGTGCAAAGCCATGTACCAGCCCACACGCAGTTGGCGTGGGTGCTGGGCTGCTT
TACCGGCTTCGGACAGGCGTCTCGGATGACACAGTTCAAGGACAAGGCGCTGCGTCAGGGTGCCGACTCCACCACCGTCG
GCCTGTTCACCTATCCTGTGTTGCAGGCTGCCGACGTGTTGGCCTACGATACTGATCTGGTGCCCGTGGGGGAGGATCAG
CGGCAGCACTTGGAGCTTGCGCGTGACTTGGCGCAGCGGTTCAATAGCCGCTTCCCGGACACCTTTGTGGTTCCCGACAT
GTTCATTCCCAAGATGGCTGCCAAGATCTACGATTTGGCCGACCCAACGTCGAAAATGAGTAAATCGGCGTCCAGTGATG
CTGGGTTGATCAACCTGCTCGACGATCCGGCGTTGTCCGTCAAGAAAATTCGAGCAGCGGTGACCGACAGTGAACGGGAG
ATCCGGTATGATCCGGAGGTCAAGCCGGGTGTGTCGAACTTGTTGAACATCCAGTCCGCGGTCACCGGGGTTGATGTCGA
TACCCTTGTGCAGCGCTACGTTGGGCACGGTTATGGCGACCTGAAGAAAGACACCGCCGAGGCCGTGGTCGAGTTCGTCA
GCCCGATCAAGGACCGGGTCGACGAATTGATGGCCGATCTAACCGAATTGGAGGTTGTTCTTGCGGTCGGAGCGCAGCGT
GCTCAAGATGTGGCTGGCAAGACGATGCAGCGGGTTTACGACCGGCTGGGCTTTCTTCCGCAACGGGGGTAA

Upstream 100 bases:

>100_bases
AAGGTGGTACCCCGGGTACCGCAAGCTACCACTGGCCGGTGTCGCTGGTGTTGGCAGGCCGGCACGGATGAAAAGGCTCA
CGTGAAAAGATCGGGGCATT

Downstream 100 bases:

>100_bases
GGCTTATCGCCATGACGGAACCGGCCAACACGGGGATTATTGATCGGCTGCGGGGCCGGTTCAGATGGCTCGACCATGTT
GTGCGCGCACACCAGCGCTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSTATGFSRIFSGVQPTSDSLHLGNALGAITQWVALQYDHPDEYEAFFCVVDLHAITIAQDPETLWRRTLVTAAQYLALG
IDPGRAVVFVQSHVPAHTQLAWVLGCFTGFGQASRMTQFKDKALRQGADSTTVGLFTYPVLQAADVLAYDTDLVPVGEDQ
RQHLELARDLAQRFNSRFPDTFVVPDMFIPKMAAKIYDLADPTSKMSKSASSDAGLINLLDDPALSVKKIRAAVTDSERE
IRYDPEVKPGVSNLLNIQSAVTGVDVDTLVQRYVGHGYGDLKKDTAEAVVEFVSPIKDRVDELMADLTELEVVLAVGAQR
AQDVAGKTMQRVYDRLGFLPQRG

Sequences:

>Translated_343_residues
MSTATGFSRIFSGVQPTSDSLHLGNALGAITQWVALQYDHPDEYEAFFCVVDLHAITIAQDPETLWRRTLVTAAQYLALG
IDPGRAVVFVQSHVPAHTQLAWVLGCFTGFGQASRMTQFKDKALRQGADSTTVGLFTYPVLQAADVLAYDTDLVPVGEDQ
RQHLELARDLAQRFNSRFPDTFVVPDMFIPKMAAKIYDLADPTSKMSKSASSDAGLINLLDDPALSVKKIRAAVTDSERE
IRYDPEVKPGVSNLLNIQSAVTGVDVDTLVQRYVGHGYGDLKKDTAEAVVEFVSPIKDRVDELMADLTELEVVLAVGAQR
AQDVAGKTMQRVYDRLGFLPQRG
>Mature_342_residues
STATGFSRIFSGVQPTSDSLHLGNALGAITQWVALQYDHPDEYEAFFCVVDLHAITIAQDPETLWRRTLVTAAQYLALGI
DPGRAVVFVQSHVPAHTQLAWVLGCFTGFGQASRMTQFKDKALRQGADSTTVGLFTYPVLQAADVLAYDTDLVPVGEDQR
QHLELARDLAQRFNSRFPDTFVVPDMFIPKMAAKIYDLADPTSKMSKSASSDAGLINLLDDPALSVKKIRAAVTDSEREI
RYDPEVKPGVSNLLNIQSAVTGVDVDTLVQRYVGHGYGDLKKDTAEAVVEFVSPIKDRVDELMADLTELEVVLAVGAQRA
QDVAGKTMQRVYDRLGFLPQRG

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=332, Percent_Identity=44.578313253012, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=50.8379888268156, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789786, Length=332, Percent_Identity=49.6987951807229, Blast_Score=327, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI71982800, Length=341, Percent_Identity=35.7771260997067, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71982793, Length=341, Percent_Identity=35.7771260997067, Blast_Score=203, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6320474, Length=347, Percent_Identity=36.5994236311239, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24666151, Length=331, Percent_Identity=41.0876132930514, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_MYCLE (Q49901)

Other databases:

- EMBL:   U00022
- EMBL:   AL583919
- PIR:   S73024
- RefSeq:   NP_301552.1
- ProteinModelPortal:   Q49901
- EnsemblBacteria:   EBMYCT00000029157
- GeneID:   909578
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0686
- NMPDR:   fig|272631.1.peg.424
- Leproma:   ML0686
- GeneTree:   EBGT00050000017280
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   MLEP272631:ML0686-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37608; Mature: 37477

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATGFSRIFSGVQPTSDSLHLGNALGAITQWVALQYDHPDEYEAFFCVVDLHAITIAQ
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEHEEEEEEC
DPETLWRRTLVTAAQYLALGIDPGRAVVFVQSHVPAHTQLAWVLGCFTGFGQASRMTQFK
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
DKALRQGADSTTVGLFTYPVLQAADVLAYDTDLVPVGEDQRQHLELARDLAQRFNSRFPD
HHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TFVVPDMFIPKMAAKIYDLADPTSKMSKSASSDAGLINLLDDPALSVKKIRAAVTDSERE
CEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCC
IRYDPEVKPGVSNLLNIQSAVTGVDVDTLVQRYVGHGYGDLKKDTAEAVVEFVSPIKDRV
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DELMADLTELEVVLAVGAQRAQDVAGKTMQRVYDRLGFLPQRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STATGFSRIFSGVQPTSDSLHLGNALGAITQWVALQYDHPDEYEAFFCVVDLHAITIAQ
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEHEEEEEEC
DPETLWRRTLVTAAQYLALGIDPGRAVVFVQSHVPAHTQLAWVLGCFTGFGQASRMTQFK
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHH
DKALRQGADSTTVGLFTYPVLQAADVLAYDTDLVPVGEDQRQHLELARDLAQRFNSRFPD
HHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TFVVPDMFIPKMAAKIYDLADPTSKMSKSASSDAGLINLLDDPALSVKKIRAAVTDSERE
CEECCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCC
IRYDPEVKPGVSNLLNIQSAVTGVDVDTLVQRYVGHGYGDLKKDTAEAVVEFVSPIKDRV
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
DELMADLTELEVVLAVGAQRAQDVAGKTMQRVYDRLGFLPQRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002