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The map label for this gene is prfB

Identifier: 221229756

GI number: 221229756

Start: 803535

End: 804659

Strand: Direct

Name: prfB

Synonym: MLBr_00667

Alternate gene names: 221229756

Gene position: 803535-804659 (Clockwise)

Preceding gene: 221229754

Following gene: 221229757

Centisome position: 24.59

GC content: 59.29

Gene sequence:

>1125_bases
GTGGAACCTGACCGACAAACCGACATCGCTGCTCTTGACTCCACCTTGACCACGGTGGAGCGGGTGCTCGACGTGGAGGG
TCTGCGCACCCGCATCGAGAAACTTGAGCATGAGGCGTCCGATCCGAAACTGTGGGATGACCAAGTCCGCGCGCAACGTG
TGACCAGCGAGTTATCTCACGCTCAGGGCGAGCTGCGCCGAATCGAGGAGCTGCGGCGACGTTTAGATGACTTGCCGGTA
CTCTACGAATTAGCGGCTGAGGAAAGGGCTGCGGCCGCCGCTAGCGGAATGGAGGCCTTCGCTGAGGCTGATGCCGAACT
CAAGGCGCTGCGCGTCGATATCGAAGCCACCGAGGTGCGCACGCTGTTGTCAGGCGAATATGACGAGCGCGAAGCGCTGA
TCACCATACGTTCCGGTGCTGGCGGAGTGGATGCCGCGGACTGGGCCGAAATGCTGATGCGGATGTATATTCGATGGGCA
GAGCAGCACAAATACGGCGTCGAGGTGTTAGACACCTCCTATGCCGAAGAAGCCGGTGTCAAAAGTGCCACGTTCGCCGT
GCACGCGCCGTTCGCCTATGGCACGTTGGCGAGCGAGCAGGGCACCCACCGGCTGGTCCGGATCAGCCCGTTCGACAACC
AGAGTCGGCGGCAGACCTCGTTCGCTGAGGTCGAAGTGTTGCCTGTGGTGGAGATCACCGATCACATCGACATTCCCGAA
GGCGATGTGCGCGTCGATGTCTACCGATCCAGCGGTCCTGGCGGCCAGTCGGTCAACACCACCGACTCTGCGGTACGGCT
AACTCACGTCCCAACCGGTCTTGTCGTTACCTGCCAGAACGAGAAATCGCAGCTGCAGAACAAGGTATCGGCGATGCGCG
TGCTGCAAGCAAAGTTGTTGGAGCGTAAGCGTTTAGAAGAACGCGCTGAGCTAGATGCATTAAAGGGTAGGGGTGGCAGC
TCGTGGGGCAATCAGATACGGTCCTATGTACTGCACCCTTATCAGATGGTCAAGGATCTGCGAAACGAGTACGAGGTGGG
TAACCCGACAGCTGTACTTGACGGAGACATCGACGGGTTCCTGGAAGCCGGGATTCGTTGGCGCAACAGACGTGATATCA
GCTAA

Upstream 100 bases:

>100_bases
GCCGCTCACATGACGATCGCTTTGATACTAGTGGCTGTTACTGGGAGGTCCCCAGAGCACTGCTGGTCGCCACCGTAGGG
CGCCGGTAACGTGGGCGGCT

Downstream 100 bases:

>100_bases
CACGACAGTTCTTGCTGTTTCGTTAACGCAAGCGTTGGCATGACTTCTGGCGCGGTGAGATCAGTGACTGGATCATCGAC
AAGAGCCTGCGGATCGTCAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MEPDRQTDIAALDSTLTTVERVLDVEGLRTRIEKLEHEASDPKLWDDQVRAQRVTSELSHAQGELRRIEELRRRLDDLPV
LYELAAEERAAAAASGMEAFAEADAELKALRVDIEATEVRTLLSGEYDEREALITIRSGAGGVDAADWAEMLMRMYIRWA
EQHKYGVEVLDTSYAEEAGVKSATFAVHAPFAYGTLASEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVEITDHIDIPE
GDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGLVVTCQNEKSQLQNKVSAMRVLQAKLLERKRLEERAELDALKGRGGS
SWGNQIRSYVLHPYQMVKDLRNEYEVGNPTAVLDGDIDGFLEAGIRWRNRRDIS

Sequences:

>Translated_374_residues
MEPDRQTDIAALDSTLTTVERVLDVEGLRTRIEKLEHEASDPKLWDDQVRAQRVTSELSHAQGELRRIEELRRRLDDLPV
LYELAAEERAAAAASGMEAFAEADAELKALRVDIEATEVRTLLSGEYDEREALITIRSGAGGVDAADWAEMLMRMYIRWA
EQHKYGVEVLDTSYAEEAGVKSATFAVHAPFAYGTLASEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVEITDHIDIPE
GDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGLVVTCQNEKSQLQNKVSAMRVLQAKLLERKRLEERAELDALKGRGGS
SWGNQIRSYVLHPYQMVKDLRNEYEVGNPTAVLDGDIDGFLEAGIRWRNRRDIS
>Mature_374_residues
MEPDRQTDIAALDSTLTTVERVLDVEGLRTRIEKLEHEASDPKLWDDQVRAQRVTSELSHAQGELRRIEELRRRLDDLPV
LYELAAEERAAAAASGMEAFAEADAELKALRVDIEATEVRTLLSGEYDEREALITIRSGAGGVDAADWAEMLMRMYIRWA
EQHKYGVEVLDTSYAEEAGVKSATFAVHAPFAYGTLASEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVEITDHIDIPE
GDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGLVVTCQNEKSQLQNKVSAMRVLQAKLLERKRLEERAELDALKGRGGS
SWGNQIRSYVLHPYQMVKDLRNEYEVGNPTAVLDGDIDGFLEAGIRWRNRRDIS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=235, Percent_Identity=35.3191489361702, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI34577120, Length=237, Percent_Identity=34.1772151898734, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=34.8993288590604, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=43.4782608695652, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=37.8600823045267, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542784, Length=170, Percent_Identity=35.8823529411765, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=32.9073482428115, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19921226, Length=294, Percent_Identity=32.9931972789116, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_MYCLB (B8ZUV0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503172.1
- ProteinModelPortal:   B8ZUV0
- SMR:   B8ZUV0
- EnsemblBacteria:   EBMYCT00000086252
- GeneID:   7325309
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015865
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41872; Mature: 41872

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPDRQTDIAALDSTLTTVERVLDVEGLRTRIEKLEHEASDPKLWDDQVRAQRVTSELSH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AQGELRRIEELRRRLDDLPVLYELAAEERAAAAASGMEAFAEADAELKALRVDIEATEVR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHH
TLLSGEYDEREALITIRSGAGGVDAADWAEMLMRMYIRWAEQHKYGVEVLDTSYAEEAGV
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHCCC
KSATFAVHAPFAYGTLASEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVEITDHIDIPE
CCEEEEEECCCHHCCCCCCCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEEECCCCCCCC
GDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGLVVTCQNEKSQLQNKVSAMRVLQAKLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERKRLEERAELDALKGRGGSSWGNQIRSYVLHPYQMVKDLRNEYEVGNPTAVLDGDIDGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCHHHH
LEAGIRWRNRRDIS
HHHCCCCCCCCCCC
>Mature Secondary Structure
MEPDRQTDIAALDSTLTTVERVLDVEGLRTRIEKLEHEASDPKLWDDQVRAQRVTSELSH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AQGELRRIEELRRRLDDLPVLYELAAEERAAAAASGMEAFAEADAELKALRVDIEATEVR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHH
TLLSGEYDEREALITIRSGAGGVDAADWAEMLMRMYIRWAEQHKYGVEVLDTSYAEEAGV
HHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHCCC
KSATFAVHAPFAYGTLASEQGTHRLVRISPFDNQSRRQTSFAEVEVLPVVEITDHIDIPE
CCEEEEEECCCHHCCCCCCCCCCEEEEECCCCCCHHHHCCHHEEEEEEEEEECCCCCCCC
GDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGLVVTCQNEKSQLQNKVSAMRVLQAKLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERKRLEERAELDALKGRGGSSWGNQIRSYVLHPYQMVKDLRNEYEVGNPTAVLDGDIDGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCCHHHH
LEAGIRWRNRRDIS
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA