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The map label for this gene is fadE23 [H]

Identifier: 221229750

GI number: 221229750

Start: 796056

End: 797258

Strand: Reverse

Name: fadE23 [H]

Synonym: MLBr_00660

Alternate gene names: 221229750

Gene position: 797258-796056 (Counterclockwise)

Preceding gene: 221229751

Following gene: 221229749

Centisome position: 24.4

GC content: 58.1

Gene sequence:

>1203_bases
ATGGCAATCAACCTGGAGCTATCGCGCAAGCTGCAAGCGGTAATCGTTAAGACCCATCAGGGCGCCGCGGAATTGATGAG
GCCGATCGCCCGCAAGTACGACTTGAAGGAACATACCTACCCAGTCGAGCTAGACACCCTGTTCAATCTGTTTGCGGGAG
CAGCCGAATCGTTCGCCTTTGCCGGCGCCGACGCGCTCGGCGATGAGGACAACAAAGACGAAAATCACAACGGCGCCAAC
ATGGCCGCGCTGCTACAAACCCTGGAGGCCTGCTGGGGCGACGTCGCGATGTTACTGTCCATACCGTATCAGGGTCTGGG
CAACGCAGCCATCTCCGCAGTAGCCACCAACAAGCAGCTGGAACGCTTAGGCAAGGTGTGGGCGGCTATGGCCATCACCG
AGCCGGGGTTTGGGTCGGACTCGGCAGCGGTGTCGACGACCGCCACCCTCGACGGTGACGAGTATGTGATTAACGGTGAA
AAGATCTTTGTTACCGCCGGGTCGCGCGCCACCCACATCGTGGTGTGGGCAACCCTAGACAAGTCGCTAGGCCACGCGGC
GATAAAGTCATTCATCGTGCCACGCGAACATCCCGGTGTCACAGTCGAACGCCTCGAGTACAAGCTAGGCATAAGGGGAT
CGGATACCGCTGCGATTCGATTTGATAACGTCCGAATTCCTAAAGACAACCTGCTAGGTAACCCAGAAATCGAGGTTGGC
AAGGGTTTTTCCGGAGTGATGGAGACTTTCGACAACACCCGGCCAATCGTTGCTGCCATGGCCGTCGGGGTTGGCCGCGC
CGCGCTGGAGGAAATCCGCAAAATCCTCACCGATGCCGGTATAGAAATTTGCTACGACAAGCCCTCGCACTCCCAGAACG
CCGCCGCGGCAGAGTTCCTGCGGATGGAAGCCGACTGGGAAGCGAGTTACCTGCTGTCGCTGCGTGCGGCGTGGCAAGCC
GACAACAACATCCCCAACTCCAAAGAAGCATCGATGAGCAAGGCCAAAGCCGGCAGAATGGCCAGCGACGTTACCCTCAA
GGCTGTCGAATTAGCAGGCACCGCAGGCTATTCCGAGAAGGCCCTGTTGGAAAAGTGGGCCCGCGACTCCAAGATCTTAG
ACATCTTCGAGGGCACTCAGCAGATTCAGCAGCTGGTTGTTGCACGCCGATTGCTGGGCTTGTCCTCTTCCGAACTCAAG
TAA

Upstream 100 bases:

>100_bases
ACGCTGGTATCGCGATCTGCGGGCGATCGGTGTCGCCGAGGGCGTCGTAGTTATCTAGGGAATCTAAAATCTGCATCTTC
TTCAAAGCGAAAGATCAATC

Downstream 100 bases:

>100_bases
ATTCCCAGACTTATCGAACCAACCTAGTCGGTGTATCCCGCACACAGACGATCTCGATGCGTTTCAAGCGCATACAGCAG
AGATGCTCACGGGTGATATT

Product: putative acyl-CoA dehydrogenase

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAINLELSRKLQAVIVKTHQGAAELMRPIARKYDLKEHTYPVELDTLFNLFAGAAESFAFAGADALGDEDNKDENHNGAN
MAALLQTLEACWGDVAMLLSIPYQGLGNAAISAVATNKQLERLGKVWAAMAITEPGFGSDSAAVSTTATLDGDEYVINGE
KIFVTAGSRATHIVVWATLDKSLGHAAIKSFIVPREHPGVTVERLEYKLGIRGSDTAAIRFDNVRIPKDNLLGNPEIEVG
KGFSGVMETFDNTRPIVAAMAVGVGRAALEEIRKILTDAGIEICYDKPSHSQNAAAAEFLRMEADWEASYLLSLRAAWQA
DNNIPNSKEASMSKAKAGRMASDVTLKAVELAGTAGYSEKALLEKWARDSKILDIFEGTQQIQQLVVARRLLGLSSSELK

Sequences:

>Translated_400_residues
MAINLELSRKLQAVIVKTHQGAAELMRPIARKYDLKEHTYPVELDTLFNLFAGAAESFAFAGADALGDEDNKDENHNGAN
MAALLQTLEACWGDVAMLLSIPYQGLGNAAISAVATNKQLERLGKVWAAMAITEPGFGSDSAAVSTTATLDGDEYVINGE
KIFVTAGSRATHIVVWATLDKSLGHAAIKSFIVPREHPGVTVERLEYKLGIRGSDTAAIRFDNVRIPKDNLLGNPEIEVG
KGFSGVMETFDNTRPIVAAMAVGVGRAALEEIRKILTDAGIEICYDKPSHSQNAAAAEFLRMEADWEASYLLSLRAAWQA
DNNIPNSKEASMSKAKAGRMASDVTLKAVELAGTAGYSEKALLEKWARDSKILDIFEGTQQIQQLVVARRLLGLSSSELK
>Mature_399_residues
AINLELSRKLQAVIVKTHQGAAELMRPIARKYDLKEHTYPVELDTLFNLFAGAAESFAFAGADALGDEDNKDENHNGANM
AALLQTLEACWGDVAMLLSIPYQGLGNAAISAVATNKQLERLGKVWAAMAITEPGFGSDSAAVSTTATLDGDEYVINGEK
IFVTAGSRATHIVVWATLDKSLGHAAIKSFIVPREHPGVTVERLEYKLGIRGSDTAAIRFDNVRIPKDNLLGNPEIEVGK
GFSGVMETFDNTRPIVAAMAVGVGRAALEEIRKILTDAGIEICYDKPSHSQNAAAAEFLRMEADWEASYLLSLRAAWQAD
NNIPNSKEASMSKAKAGRMASDVTLKAVELAGTAGYSEKALLEKWARDSKILDIFEGTQQIQQLVVARRLLGLSSSELK

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI187960098, Length=402, Percent_Identity=32.8358208955224, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4557231, Length=402, Percent_Identity=32.8358208955224, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4557233, Length=393, Percent_Identity=32.3155216284987, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4501859, Length=264, Percent_Identity=31.4393939393939, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI21361497, Length=307, Percent_Identity=30.2931596091205, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI76496475, Length=300, Percent_Identity=30.3333333333333, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4557235, Length=300, Percent_Identity=30.3333333333333, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI226958414, Length=279, Percent_Identity=28.3154121863799, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4501857, Length=278, Percent_Identity=26.978417266187, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI226958412, Length=305, Percent_Identity=27.2131147540984, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7656849, Length=268, Percent_Identity=25.7462686567164, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4503943, Length=279, Percent_Identity=24.0143369175627, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI48976061, Length=277, Percent_Identity=25.9927797833935, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI211971008, Length=277, Percent_Identity=25.9927797833935, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=329, Percent_Identity=27.6595744680851, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081958, Length=265, Percent_Identity=28.6792452830189, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17570075, Length=272, Percent_Identity=38.2352941176471, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17534899, Length=399, Percent_Identity=31.8295739348371, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17569725, Length=276, Percent_Identity=37.6811594202899, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17508101, Length=269, Percent_Identity=34.2007434944238, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17506239, Length=278, Percent_Identity=33.0935251798561, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71985184, Length=299, Percent_Identity=30.4347826086957, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71990804, Length=249, Percent_Identity=32.5301204819277, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17531909, Length=246, Percent_Identity=26.8292682926829, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538396, Length=307, Percent_Identity=26.3843648208469, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32563615, Length=155, Percent_Identity=38.0645161290323, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985192, Length=181, Percent_Identity=34.8066298342541, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533517, Length=275, Percent_Identity=26.5454545454545, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505929, Length=274, Percent_Identity=27.007299270073, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17534353, Length=341, Percent_Identity=24.9266862170088, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551932, Length=268, Percent_Identity=27.9850746268657, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982178, Length=155, Percent_Identity=31.6129032258064, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21356377, Length=273, Percent_Identity=38.0952380952381, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24660351, Length=339, Percent_Identity=35.3982300884956, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24646207, Length=407, Percent_Identity=29.7297297297297, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24666513, Length=291, Percent_Identity=33.6769759450172, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI281363737, Length=322, Percent_Identity=29.1925465838509, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21355753, Length=313, Percent_Identity=27.7955271565495, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19920834, Length=282, Percent_Identity=26.9503546099291, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281365206, Length=337, Percent_Identity=27.0029673590504, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585193, Length=337, Percent_Identity=27.0029673590504, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585195, Length=337, Percent_Identity=27.0029673590504, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585191, Length=337, Percent_Identity=27.0029673590504, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 43150; Mature: 43018

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINLELSRKLQAVIVKTHQGAAELMRPIARKYDLKEHTYPVELDTLFNLFAGAAESFAF
CEEECHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHH
AGADALGDEDNKDENHNGANMAALLQTLEACWGDVAMLLSIPYQGLGNAAISAVATNKQL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ERLGKVWAAMAITEPGFGSDSAAVSTTATLDGDEYVINGEKIFVTAGSRATHIVVWATLD
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEECCCEEEEEECCCCEEEEEEEECC
KSLGHAAIKSFIVPREHPGVTVERLEYKLGIRGSDTAAIRFDNVRIPKDNLLGNPEIEVG
HHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCCEEEEECCEECCHHHCCCCCCEEEC
KGFSGVMETFDNTRPIVAAMAVGVGRAALEEIRKILTDAGIEICYDKPSHSQNAAAAEFL
CCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
RMEADWEASYLLSLRAAWQADNNIPNSKEASMSKAKAGRMASDVTLKAVELAGTAGYSEK
HHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHH
ALLEKWARDSKILDIFEGTQQIQQLVVARRLLGLSSSELK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AINLELSRKLQAVIVKTHQGAAELMRPIARKYDLKEHTYPVELDTLFNLFAGAAESFAF
EEECHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHH
AGADALGDEDNKDENHNGANMAALLQTLEACWGDVAMLLSIPYQGLGNAAISAVATNKQL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ERLGKVWAAMAITEPGFGSDSAAVSTTATLDGDEYVINGEKIFVTAGSRATHIVVWATLD
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEECCCEEEEEECCCCEEEEEEEECC
KSLGHAAIKSFIVPREHPGVTVERLEYKLGIRGSDTAAIRFDNVRIPKDNLLGNPEIEVG
HHHHHHHHHHHCCCCCCCCCCHHHHHEEECCCCCCCEEEEECCEECCHHHCCCCCCEEEC
KGFSGVMETFDNTRPIVAAMAVGVGRAALEEIRKILTDAGIEICYDKPSHSQNAAAAEFL
CCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
RMEADWEASYLLSLRAAWQADNNIPNSKEASMSKAKAGRMASDVTLKAVELAGTAGYSEK
HHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHH
ALLEKWARDSKILDIFEGTQQIQQLVVARRLLGLSSSELK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]