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The map label for this gene is subI [H]

Identifier: 221229730

GI number: 221229730

Start: 748391

End: 749437

Strand: Direct

Name: subI [H]

Synonym: MLBr_00615

Alternate gene names: 221229730

Gene position: 748391-749437 (Clockwise)

Preceding gene: 221229729

Following gene: 221229731

Centisome position: 22.9

GC content: 59.6

Gene sequence:

>1047_bases
ATGTTTAAAGACATTGGGCGATTCATGCGCTGGCGGCACTCGATGGTCGTCGGGGTCACGCTTGCCTCGACCACCGATGT
GGTTGCTGCGTGTCATGGTGGTGCCAGTGACGTCGTTGGCGGCGGGACGGCCCATGCGCATACGAGTATCGCCCTGGTCG
CTTACTCGGCGCCGGGACCTGGTTGGGGCAAAGTGATTCCGGCGTTTAATGACGCTCGGTCGGGCGATGGTGTGCAAGTG
GTAACGTCGTATGGAGCCTCTGGCGACCAGTCCCGCGGTGTTGTCGATGGCAAGCCAGCTGACATCGTGAATTTCTCGGT
TGAAACGGACATCACCAGATTGGTCAAGGTAGGCAAGGTGTCCAAGGACTGGGATACCGACGCCACGAAGGGCATCCCGT
TCGGATCAGTGGTGACATTGGTTGTGCGCACCGGTAATCCCAAGAACATCAAAGATTGGGATGATCTGGTGCGCCCCGGT
GTGGAGGTGATCATGCCCAATCCGCTTAGCTCGGGCTCGGCCAAGTGGAATCTGCTTGCTCCGTACGCCGCCAAAAGCGA
AGGTTGCCGCAATAGCCAGGCGGGGATTGATTTTGTGAACCGGTTGGTGGCCGAACACGTCAAATTGCGGCTAGGATCTG
CGCGCGAAGCTACCGATGCCTTTGTCCAGGGCAGCGGCGATGTGCTGATCAGCTATGAGAACGAGGCTATCGCCGCCGAG
CGGCAGGGCAAGCCGGTCGAACACATCAACCCGCCGGTTACCTTCAAGATCGAAAATCCGTTGGCGGTGGTGAGCACCAG
CGCGCACCTCGAGGTCGCGACCGCGTTCAAGAACTTCCAATACACCACTGCCGCGCAGGGGTTGTGGGCGCAGGCAGGTT
TTCGGCCGGTGGATCCGGCGGTCAGTGCCGATTTCAGCGACCAATTCCCGGCACCGGTGAAACTGTGGACGATCGCCGAC
TTGGGTGGCTGGAGCACGGTGGATCCGCAGCTATTCGACAAGAACACCGGTAGCATCACCAAGGTCTATATGCAGGCCAC
CGGATGA

Upstream 100 bases:

>100_bases
GCGCGGTCTGGTACGGAGTTCGTGAATTTTCCCTAGTTCTAGGTGGCCCTTGCGTACCAACGGGATTGAGATGCCTACGT
TCCTCACTGGAAGGCGGCTA

Downstream 100 bases:

>100_bases
CGGCTCGTTAATCGATACTCGTTCGTTGTGTCGGCTCGGTACTACCACGTAGCTTGGGGTTGGTGCGGCGATAGTGTGGG
CTTTCGGTGATCGTGCTGCT

Product: putative sulphate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MFKDIGRFMRWRHSMVVGVTLASTTDVVAACHGGASDVVGGGTAHAHTSIALVAYSAPGPGWGKVIPAFNDARSGDGVQV
VTSYGASGDQSRGVVDGKPADIVNFSVETDITRLVKVGKVSKDWDTDATKGIPFGSVVTLVVRTGNPKNIKDWDDLVRPG
VEVIMPNPLSSGSAKWNLLAPYAAKSEGCRNSQAGIDFVNRLVAEHVKLRLGSAREATDAFVQGSGDVLISYENEAIAAE
RQGKPVEHINPPVTFKIENPLAVVSTSAHLEVATAFKNFQYTTAAQGLWAQAGFRPVDPAVSADFSDQFPAPVKLWTIAD
LGGWSTVDPQLFDKNTGSITKVYMQATG

Sequences:

>Translated_348_residues
MFKDIGRFMRWRHSMVVGVTLASTTDVVAACHGGASDVVGGGTAHAHTSIALVAYSAPGPGWGKVIPAFNDARSGDGVQV
VTSYGASGDQSRGVVDGKPADIVNFSVETDITRLVKVGKVSKDWDTDATKGIPFGSVVTLVVRTGNPKNIKDWDDLVRPG
VEVIMPNPLSSGSAKWNLLAPYAAKSEGCRNSQAGIDFVNRLVAEHVKLRLGSAREATDAFVQGSGDVLISYENEAIAAE
RQGKPVEHINPPVTFKIENPLAVVSTSAHLEVATAFKNFQYTTAAQGLWAQAGFRPVDPAVSADFSDQFPAPVKLWTIAD
LGGWSTVDPQLFDKNTGSITKVYMQATG
>Mature_348_residues
MFKDIGRFMRWRHSMVVGVTLASTTDVVAACHGGASDVVGGGTAHAHTSIALVAYSAPGPGWGKVIPAFNDARSGDGVQV
VTSYGASGDQSRGVVDGKPADIVNFSVETDITRLVKVGKVSKDWDTDATKGIPFGSVVTLVVRTGNPKNIKDWDDLVRPG
VEVIMPNPLSSGSAKWNLLAPYAAKSEGCRNSQAGIDFVNRLVAEHVKLRLGSAREATDAFVQGSGDVLISYENEAIAAE
RQGKPVEHINPPVTFKIENPLAVVSTSAHLEVATAFKNFQYTTAAQGLWAQAGFRPVDPAVSADFSDQFPAPVKLWTIAD
LGGWSTVDPQLFDKNTGSITKVYMQATG

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=295, Percent_Identity=35.5932203389831, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1788765, Length=263, Percent_Identity=31.1787072243346, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR006311
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36884; Mature: 36884

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDIGRFMRWRHSMVVGVTLASTTDVVAACHGGASDVVGGGTAHAHTSIALVAYSAPGP
CCHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GWGKVIPAFNDARSGDGVQVVTSYGASGDQSRGVVDGKPADIVNFSVETDITRLVKVGKV
CCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCC
SKDWDTDATKGIPFGSVVTLVVRTGNPKNIKDWDDLVRPGVEVIMPNPLSSGSAKWNLLA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEC
PYAAKSEGCRNSQAGIDFVNRLVAEHVKLRLGSAREATDAFVQGSGDVLISYENEAIAAE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCEEEHH
RQGKPVEHINPPVTFKIENPLAVVSTSAHLEVATAFKNFQYTTAAQGLWAQAGFRPVDPA
CCCCCHHHCCCCEEEEECCCEEEEECCCCEEEEEHHHCCEEEHHHCCHHHHCCCCCCCCC
VSADFSDQFPAPVKLWTIADLGGWSTVDPQLFDKNTGSITKVYMQATG
CCCCCCCCCCCCEEEEEEEECCCCCCCCCHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MFKDIGRFMRWRHSMVVGVTLASTTDVVAACHGGASDVVGGGTAHAHTSIALVAYSAPGP
CCHHHHHHHHHHCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GWGKVIPAFNDARSGDGVQVVTSYGASGDQSRGVVDGKPADIVNFSVETDITRLVKVGKV
CCCCEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCC
SKDWDTDATKGIPFGSVVTLVVRTGNPKNIKDWDDLVRPGVEVIMPNPLSSGSAKWNLLA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEC
PYAAKSEGCRNSQAGIDFVNRLVAEHVKLRLGSAREATDAFVQGSGDVLISYENEAIAAE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCEEEHH
RQGKPVEHINPPVTFKIENPLAVVSTSAHLEVATAFKNFQYTTAAQGLWAQAGFRPVDPA
CCCCCHHHCCCCEEEEECCCEEEEECCCCEEEEEHHHCCEEEHHHCCHHHHCCCCCCCCC
VSADFSDQFPAPVKLWTIADLGGWSTVDPQLFDKNTGSITKVYMQATG
CCCCCCCCCCCCEEEEEEEECCCCCCCCCHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]