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The map label for this gene is carA

Identifier: 221229669

GI number: 221229669

Start: 648431

End: 649558

Strand: Direct

Name: carA

Synonym: MLBr_00535

Alternate gene names: 221229669

Gene position: 648431-649558 (Clockwise)

Preceding gene: 221229668

Following gene: 221229670

Centisome position: 19.84

GC content: 60.02

Gene sequence:

>1128_bases
GTGAGCAAAGCCCTACTGGTTCTCGAAGACGGCCGCGTCTTCACCGGCATGCCGTTTGGTGCGATTGGCCAAACGTTGGG
TGAAGCCGTGTTTTCCACCGGCATGTCCGGCTACCAGGAGACGTTGACCGATCCCAGTTATCACCGTCAGATCGTGGTGG
CCACCGCCCCGCAGATCGGCAACACCGGCTGGAACGACCAGGACGGTGAGAGTCGCGACGATAGAATCTGGGTCGCGGGT
TACGCGGTGCGTGACCCGTCTCCGTGCGCCTCGAACTGGCGCGCCACCGGCACGCTGGAAGACGAGCTGGTCCGCCAGCG
TATTGTTGGGATCGCCGGTATCGACACTCGGGCGGTAGTGCGTCATCTGCGCAGATACGGTTCGATGAAGGCGGGTGTGT
TTTCTGGCAAGGCAATGATCGAGCCCCTAAATATCGAGGCGCTAGTACAACATGTGCGCACCCAGCAGTCGATGCTGGGT
GCTGATCTTGTTGGCGAAGCCAGCACACTGGATGCATATGTTGTTGAACCCAAAGGGAAGGAACGCTTTACGGTGGCAGC
GCTTGACCTCGGGATCAAGACCAACACGCCGCGCAACTTCGCTCGGCGTGGGATTCGCAGCTATGTGCTGCCGGCGTCGG
CGACGTTCAACCAAATTGCCGACATCAAACCGCATGGCCTGTTTTTGTCCAATGGCCCTGGTGACCCGGCTACCGCCGAC
CACGTCGTTGCGCTCACTCGAGAGGTGCTGAACGCCGGAATTCCTTTGTTCGGTATCTGTTTCGGCAACCAGATCTTGGG
CCGGGCGCTAGGACTGTCGACCTACAAGATGGTCTTCGGCCACCGCGGTATCAATATACCGGTCATCGACCACGCCACCG
GACGGGTCGCGGTGACCGCGCAAAACCATGGTTTCGCACTGGAAGGGGAGGCAGGCCAGTCCTTCGACACACCTTTTGGC
TCAGCGGTGGTCAGTCACACCTGTGCCAACGATGGGGTGGTGGAAGGTGTCAAACTCGCTGACGGGCGTGCATTTTCGGT
GCAATATCACCCGGAGGCGGCCGCCGGCCCGCACGATGCCGAGTACCTATTCGATAGTTTTGTCGAACTCATGGCAGCCC
AGCGATGA

Upstream 100 bases:

>100_bases
CCTCAGACAGGAGAAGCCGCGTGAGCCGCGCCGGCGATGATGCAGAGCGAAAGAGCGATGTGGGGTCACCTACCGCTTGC
GGGAGAAAGGAGTGGCTCTC

Downstream 100 bases:

>100_bases
CCGCGAGCCCGCGCGTTTGCAAAACGACACGTCGCAATCACTGTTCAAATGAGGACGCTTGCGCCCAGAAAGGGGGACGC
TAGTGCCGCGGCGGACTGAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSKALLVLEDGRVFTGMPFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDQDGESRDDRIWVAG
YAVRDPSPCASNWRATGTLEDELVRQRIVGIAGIDTRAVVRHLRRYGSMKAGVFSGKAMIEPLNIEALVQHVRTQQSMLG
ADLVGEASTLDAYVVEPKGKERFTVAALDLGIKTNTPRNFARRGIRSYVLPASATFNQIADIKPHGLFLSNGPGDPATAD
HVVALTREVLNAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALEGEAGQSFDTPFG
SAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDAEYLFDSFVELMAAQR

Sequences:

>Translated_375_residues
MSKALLVLEDGRVFTGMPFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDQDGESRDDRIWVAG
YAVRDPSPCASNWRATGTLEDELVRQRIVGIAGIDTRAVVRHLRRYGSMKAGVFSGKAMIEPLNIEALVQHVRTQQSMLG
ADLVGEASTLDAYVVEPKGKERFTVAALDLGIKTNTPRNFARRGIRSYVLPASATFNQIADIKPHGLFLSNGPGDPATAD
HVVALTREVLNAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALEGEAGQSFDTPFG
SAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDAEYLFDSFVELMAAQR
>Mature_374_residues
SKALLVLEDGRVFTGMPFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDQDGESRDDRIWVAGY
AVRDPSPCASNWRATGTLEDELVRQRIVGIAGIDTRAVVRHLRRYGSMKAGVFSGKAMIEPLNIEALVQHVRTQQSMLGA
DLVGEASTLDAYVVEPKGKERFTVAALDLGIKTNTPRNFARRGIRSYVLPASATFNQIADIKPHGLFLSNGPGDPATADH
VVALTREVLNAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALEGEAGQSFDTPFGS
AVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDAEYLFDSFVELMAAQR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=40.8376963350785, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI21361331, Length=380, Percent_Identity=35.5263157894737, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI169790915, Length=380, Percent_Identity=35.5263157894737, Blast_Score=195, Evalue=7e-50,
Organism=Escherichia coli, GI1786215, Length=389, Percent_Identity=42.9305912596401, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI193204318, Length=380, Percent_Identity=38.6842105263158, Blast_Score=227, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=36.1596009975062, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324878, Length=383, Percent_Identity=35.2480417754569, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=39.7932816537468, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=39.7932816537468, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_MYCLE (Q9CCR3)

Other databases:

- EMBL:   AL583918
- PIR:   G86975
- RefSeq:   NP_301454.1
- ProteinModelPortal:   Q9CCR3
- SMR:   Q9CCR3
- EnsemblBacteria:   EBMYCT00000027924
- GeneID:   909297
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0535
- NMPDR:   fig|272631.1.peg.326
- Leproma:   ML0535
- GeneTree:   EBGT00050000017681
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   MLEP272631:ML0535-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40167; Mature: 40036

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 260-260 ACT_SITE 350-350 ACT_SITE 352-352

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALLVLEDGRVFTGMPFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIG
CCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCC
NTGWNDQDGESRDDRIWVAGYAVRDPSPCASNWRATGTLEDELVRQRIVGIAGIDTRAVV
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHH
RHLRRYGSMKAGVFSGKAMIEPLNIEALVQHVRTQQSMLGADLVGEASTLDAYVVEPKGK
HHHHHHCCCCCCEECCCCEECCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCCCC
ERFTVAALDLGIKTNTPRNFARRGIRSYVLPASATFNQIADIKPHGLFLSNGPGDPATAD
CEEEEEEEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHCCCCCCEEEECCCCCCCCHH
HVVALTREVLNAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTA
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
QNHGFALEGEAGQSFDTPFGSAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDA
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCHH
EYLFDSFVELMAAQR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKALLVLEDGRVFTGMPFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIG
CCEEEEEECCCEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCC
NTGWNDQDGESRDDRIWVAGYAVRDPSPCASNWRATGTLEDELVRQRIVGIAGIDTRAVV
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHH
RHLRRYGSMKAGVFSGKAMIEPLNIEALVQHVRTQQSMLGADLVGEASTLDAYVVEPKGK
HHHHHHCCCCCCEECCCCEECCCCHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCCCC
ERFTVAALDLGIKTNTPRNFARRGIRSYVLPASATFNQIADIKPHGLFLSNGPGDPATAD
CEEEEEEEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHCCCCCCEEEECCCCCCCCHH
HVVALTREVLNAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTA
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
QNHGFALEGEAGQSFDTPFGSAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDA
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCHH
EYLFDSFVELMAAQR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002