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The map label for this gene is pyrB

Identifier: 221229667

GI number: 221229667

Start: 645590

End: 646555

Strand: Direct

Name: pyrB

Synonym: MLBr_00532

Alternate gene names: 221229667

Gene position: 645590-646555 (Clockwise)

Preceding gene: 221229665

Following gene: 221229668

Centisome position: 19.75

GC content: 62.22

Gene sequence:

>966_bases
GTGATGCGCAGACATCTGCTGAACGCCGGTGATTTGAGCCGCGACGATGCCATCGCCATCTTCGACGATGCCGACCGGTT
CGCGCATGCTCTGGTGGGTCGCGAGATCAAGAAGCTGCCGACGCTACGAGGTCGCACGGTAATCACGATGTTCTACGAAA
ATTCGACTCGCACCCGCGTGTCGTTCGAGGTGGCCGGCAAGTGGATGAGCGCCAATGTGATCAACGTCAGCCCGGCTGGA
TCGTCGGTGGGTAAGGGCGAATCGTTGCGTGACACCGCGCTGACACTGCGTGCGGCCGGTGCTGACGCGTTGATTATCCG
GCACCCGGCTTCCGGTGCTGCCCATCTGCTTGCCGATTGGACTGCTGCTGAGACCGGCGAGGATGGTCCGGCGGTGATCA
ACGCCGGCGACGGCACTCATGAGCACCCGACCCAGGCGCTGCTGGACGCGCTCACCATCCGTCAGCGGCTCGGTAGCATT
GAAGGCCGGCGCATCTTGATCGTCGGCGACATCTTGCACAGCCGGGTTGCGCGGTCCAACGTCGTGCTGCTAGACACCCT
GGGTGCTGAGGTGGTGCTGGTGGCGCCGCGGACTTTGTTGCCGATTGGGGTTGACGGCTGGCCAGCCACCGTCTCATGCG
ACTTTGACGCTGAACTGCCTGCCGCCGATGCGGTGCTGATGCTGCGAGTACAGGCCGAACGGATGAATGGCGGGTTTTTC
CCGTCGGTACGCGAGTATTCTTGTCTCTACGGGCTAACCGAGCGGCGCCAGGCACTGTTGCCCGGCCATGCAGTGGTGCT
GCACCCAGGGCCGATGCTACGTGGCATGGAGATCGCCTCTTCTGTTGCTGACTCGTCGCAATCAGCTGTTTTGCAACAGG
TTTCCAACGGTGTGCATGTGCGGATGGCAGTGCTTTTCCATGTTCTGGTTGGGACCCAGTCGGCCGAAGAAGAGGGTGCG
GCATGA

Upstream 100 bases:

>100_bases
ACGTGGGCAAGAACGTGCCTACCTCGCACCGTGAGAGTGTGCATGTACTGCTGTGTGAGCACGACGGCAGCGACGGCGTG
GTGATATCCAGGGACGGTGC

Downstream 100 bases:

>100_bases
GCGTGTTACTTCGTGGTGTCCGCTTGTATGGCGAGGGTGAACAAGTCGATGTACTGGTTGAGGGCGAGCAGATCGCCAAC
ATTGGTGCTGGTATTGATAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MMRRHLLNAGDLSRDDAIAIFDDADRFAHALVGREIKKLPTLRGRTVITMFYENSTRTRVSFEVAGKWMSANVINVSPAG
SSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLADWTAAETGEDGPAVINAGDGTHEHPTQALLDALTIRQRLGSI
EGRRILIVGDILHSRVARSNVVLLDTLGAEVVLVAPRTLLPIGVDGWPATVSCDFDAELPAADAVLMLRVQAERMNGGFF
PSVREYSCLYGLTERRQALLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGTQSAEEEGA
A

Sequences:

>Translated_321_residues
MMRRHLLNAGDLSRDDAIAIFDDADRFAHALVGREIKKLPTLRGRTVITMFYENSTRTRVSFEVAGKWMSANVINVSPAG
SSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLADWTAAETGEDGPAVINAGDGTHEHPTQALLDALTIRQRLGSI
EGRRILIVGDILHSRVARSNVVLLDTLGAEVVLVAPRTLLPIGVDGWPATVSCDFDAELPAADAVLMLRVQAERMNGGFF
PSVREYSCLYGLTERRQALLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGTQSAEEEGA
A
>Mature_321_residues
MMRRHLLNAGDLSRDDAIAIFDDADRFAHALVGREIKKLPTLRGRTVITMFYENSTRTRVSFEVAGKWMSANVINVSPAG
SSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLADWTAAETGEDGPAVINAGDGTHEHPTQALLDALTIRQRLGSI
EGRRILIVGDILHSRVARSNVVLLDTLGAEVVLVAPRTLLPIGVDGWPATVSCDFDAELPAADAVLMLRVQAERMNGGFF
PSVREYSCLYGLTERRQALLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHVRMAVLFHVLVGTQSAEEEGA
A

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=316, Percent_Identity=37.0253164556962, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=31.3479623824451, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786469, Length=344, Percent_Identity=25.2906976744186, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790703, Length=307, Percent_Identity=26.3843648208469, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=334, Percent_Identity=34.1317365269461, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=317, Percent_Identity=33.4384858044164, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24642586, Length=328, Percent_Identity=33.5365853658537, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_MYCLB (B8ZUL1)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503083.1
- ProteinModelPortal:   B8ZUL1
- SMR:   B8ZUL1
- EnsemblBacteria:   EBMYCT00000086001
- GeneID:   7326274
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016463
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34258; Mature: 34258

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRHLLNAGDLSRDDAIAIFDDADRFAHALVGREIKKLPTLRGRTVITMFYENSTRTRV
CCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
SFEVAGKWMSANVINVSPAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLADW
EEEEECCEEECEEEEECCCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCCHHHEECC
TAAETGEDGPAVINAGDGTHEHPTQALLDALTIRQRLGSIEGRRILIVGDILHSRVARSN
HHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCC
VVLLDTLGAEVVLVAPRTLLPIGVDGWPATVSCDFDAELPAADAVLMLRVQAERMNGGFF
EEEEEECCCCEEEECCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEEEHHHCCCCCC
PSVREYSCLYGLTERRQALLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHV
CCHHHHHHHHCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEE
RMAVLFHVLVGTQSAEEEGAA
HHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MMRRHLLNAGDLSRDDAIAIFDDADRFAHALVGREIKKLPTLRGRTVITMFYENSTRTRV
CCHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEE
SFEVAGKWMSANVINVSPAGSSVGKGESLRDTALTLRAAGADALIIRHPASGAAHLLADW
EEEEECCEEECEEEEECCCCCCCCCCCCCHHHEEEEEECCCCEEEEECCCCCCHHHEECC
TAAETGEDGPAVINAGDGTHEHPTQALLDALTIRQRLGSIEGRRILIVGDILHSRVARSN
HHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCC
VVLLDTLGAEVVLVAPRTLLPIGVDGWPATVSCDFDAELPAADAVLMLRVQAERMNGGFF
EEEEEECCCCEEEECCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEEEHHHCCCCCC
PSVREYSCLYGLTERRQALLPGHAVVLHPGPMLRGMEIASSVADSSQSAVLQQVSNGVHV
CCHHHHHHHHCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEE
RMAVLFHVLVGTQSAEEEGAA
HHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA