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The map label for this gene is groEL

Identifier: 221229588

GI number: 221229588

Start: 473567

End: 475180

Strand: Direct

Name: groEL

Synonym: MLBr_00381

Alternate gene names: 221229588

Gene position: 473567-475180 (Clockwise)

Preceding gene: 221229587

Following gene: 221229590

Centisome position: 14.49

GC content: 63.57

Gene sequence:

>1614_bases
ATGAGCAAGCTGATTGAGTACGACGAAACCGCGCGTCATGCCATGGAGGTGGGCATGAACAAGCTCGCCGACACGGTGCG
GGTAACACTTGGGCCGCGCGGTCGACATGTGGTGTTGGCCAAGGCATTCGGCGGGCCCACCATCACCAACGACGGTGTCA
CCGTCGCGCGTGAGATCGACCTAGAGGACCCGTTCGAAAATCTGGGCGCTCAGCTGGTGAAGTCGGTGGCCACCAAGACC
AACGACGTCGCTGGCGACGGCACTACCACCGCGACCGTGTTGGCCCAGGCGCTGGTCAAGGGCGGCCTGCGCATGGTCGC
CGCCGGGGCCAACCCGGTCGCCCTAGGTGCTGGCATCAGCAAGGCCGCCGACGCGGTGTCCGAGGCACTGCTGGCGGTAG
CAACCCCGGTGGCCGGCAAGGACGCCATCACGCAGGTTGCGACGGTTTCTTCGCGCGACGAGCAGATCGGTGCGCTGGTC
GGCGAGGGGATGAACAAGGTCGGCACTGACGGCGTTGTCAGCGTTGAAGAGTCGTCGACGCTGGACACCGAGCTAGAGTT
CACCGAGGGCGTCGGCTTCGACAAAGGTTTCCTGTCGGCGTATTTCGTGACCGACTTCGACTCGCAGCAGGCTGTGCTCG
ACGATCCGCTGGTCCTGCTGCACCAGGAGAAGATCAGCTCGCTGCCTGAGTTGCTGCCGATGCTCGAGAAGGTCACTGAG
TCAGGCAAGCCGCTGCTGATCGTCGCCGAAGACCTCGAGGGTGAAGCATTGGCGACGCTTGTCGTTAACTCGATCCGCAA
GACGCTCAAGGCGGTTGCGGTTAAGTCCCCGTTCTTCGGCGACCGACGCAAGGCCTTCCTCGAAGATTTGGCAATTGTGA
CGGGTGGTCAGGTAGTCAACCCCGAAACCGGCCTGGTGCTGCGCGAGGTGGGCACTGACGTGCTGGGCTCGGCCCGCCGT
GTGGTGGTTAGTAAGGACGACACGATCATCGTCGACGGCGGCGGTTCCAACGACGCGGTGGCCAAGCGAGTCAATCAGCT
GCGGGCCGAGATAGAGGTCAGCGACTCAGAATGGGATCGCGAAAAGCTGCAGGAGCGCGTGGCCAAACTGGCCGGTGGCG
TGGCCGTCATCAAGGTGGGGGCTGTCACCGAGACGGCGCTGAAGAAACGCAAGGAAAGCGTCGAAGACGCCGTCGCGGCT
GCGAAGGCGTCTATCGAGGAGGGTATCATCGCGGGTGGTGGATCGGCGCTCGTCCAGTGCGGCGCGGCGCTCAAGCAATT
GCGTACGTCGCTGACCGGAGACGAAGCCCTCGGCATCGACGTATTTTTCGAGGCGCTCAAGGCACCGCTTTACTGGATTG
CCACTAACGCCGGGTTGGATGGCGCGGTGGTGGTCGACAAGGTTAGCGGGCTGCCCGCCGGGCACGGACTGAATGCTAGC
ACGCTGGGCTATGGCGATTTGGTCGCCGATGGTGTCGTCGACCCGGTCAAGGTCACCAGATCAGCGGTGCTTAACGCGGC
CTCGGTGGCGCGGATGATGCTCACCACCGAAACGGCGGTGGTGGACAAGCCGGCCAAGACAGAAGAGCATGACCACCACG
GCCATGCGCACTAA

Upstream 100 bases:

>100_bases
GCTGGCTGTCGTATCCAAGTAACGAACCGTGTTCCGCCCCGGCGATCCCCGTGCTTAACCACGGGTGATTTCCGGGGCGG
CATGCGTTTAAAGGAGCCTG

Downstream 100 bases:

>100_bases
ACAGCCAGTGCGTACCCCTAGAAAGACCCCCGGATACCGGGGGTCTTTCTAGTTGATATGCGTTCGGTTTCACAGCAGAG
GGCAATACGCTGCCAAAGTT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 1; Protein Cpn60 1

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSKLIEYDETARHAMEVGMNKLADTVRVTLGPRGRHVVLAKAFGGPTITNDGVTVAREIDLEDPFENLGAQLVKSVATKT
NDVAGDGTTTATVLAQALVKGGLRMVAAGANPVALGAGISKAADAVSEALLAVATPVAGKDAITQVATVSSRDEQIGALV
GEGMNKVGTDGVVSVEESSTLDTELEFTEGVGFDKGFLSAYFVTDFDSQQAVLDDPLVLLHQEKISSLPELLPMLEKVTE
SGKPLLIVAEDLEGEALATLVVNSIRKTLKAVAVKSPFFGDRRKAFLEDLAIVTGGQVVNPETGLVLREVGTDVLGSARR
VVVSKDDTIIVDGGGSNDAVAKRVNQLRAEIEVSDSEWDREKLQERVAKLAGGVAVIKVGAVTETALKKRKESVEDAVAA
AKASIEEGIIAGGGSALVQCGAALKQLRTSLTGDEALGIDVFFEALKAPLYWIATNAGLDGAVVVDKVSGLPAGHGLNAS
TLGYGDLVADGVVDPVKVTRSAVLNAASVARMMLTTETAVVDKPAKTEEHDHHGHAH

Sequences:

>Translated_537_residues
MSKLIEYDETARHAMEVGMNKLADTVRVTLGPRGRHVVLAKAFGGPTITNDGVTVAREIDLEDPFENLGAQLVKSVATKT
NDVAGDGTTTATVLAQALVKGGLRMVAAGANPVALGAGISKAADAVSEALLAVATPVAGKDAITQVATVSSRDEQIGALV
GEGMNKVGTDGVVSVEESSTLDTELEFTEGVGFDKGFLSAYFVTDFDSQQAVLDDPLVLLHQEKISSLPELLPMLEKVTE
SGKPLLIVAEDLEGEALATLVVNSIRKTLKAVAVKSPFFGDRRKAFLEDLAIVTGGQVVNPETGLVLREVGTDVLGSARR
VVVSKDDTIIVDGGGSNDAVAKRVNQLRAEIEVSDSEWDREKLQERVAKLAGGVAVIKVGAVTETALKKRKESVEDAVAA
AKASIEEGIIAGGGSALVQCGAALKQLRTSLTGDEALGIDVFFEALKAPLYWIATNAGLDGAVVVDKVSGLPAGHGLNAS
TLGYGDLVADGVVDPVKVTRSAVLNAASVARMMLTTETAVVDKPAKTEEHDHHGHAH
>Mature_536_residues
SKLIEYDETARHAMEVGMNKLADTVRVTLGPRGRHVVLAKAFGGPTITNDGVTVAREIDLEDPFENLGAQLVKSVATKTN
DVAGDGTTTATVLAQALVKGGLRMVAAGANPVALGAGISKAADAVSEALLAVATPVAGKDAITQVATVSSRDEQIGALVG
EGMNKVGTDGVVSVEESSTLDTELEFTEGVGFDKGFLSAYFVTDFDSQQAVLDDPLVLLHQEKISSLPELLPMLEKVTES
GKPLLIVAEDLEGEALATLVVNSIRKTLKAVAVKSPFFGDRRKAFLEDLAIVTGGQVVNPETGLVLREVGTDVLGSARRV
VVSKDDTIIVDGGGSNDAVAKRVNQLRAEIEVSDSEWDREKLQERVAKLAGGVAVIKVGAVTETALKKRKESVEDAVAAA
KASIEEGIIAGGGSALVQCGAALKQLRTSLTGDEALGIDVFFEALKAPLYWIATNAGLDGAVVVDKVSGLPAGHGLNAST
LGYGDLVADGVVDPVKVTRSAVLNAASVARMMLTTETAVVDKPAKTEEHDHHGHAH

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=532, Percent_Identity=43.0451127819549, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI31542947, Length=532, Percent_Identity=43.0451127819549, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI38455427, Length=547, Percent_Identity=20.8409506398537, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI24307939, Length=575, Percent_Identity=21.3913043478261, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790586, Length=524, Percent_Identity=53.0534351145038, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=40.607210626186, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI193210679, Length=208, Percent_Identity=37.5, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144674, Length=540, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323288, Length=525, Percent_Identity=42.8571428571429, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322524, Length=102, Percent_Identity=37.2549019607843, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641193, Length=525, Percent_Identity=44.3809523809524, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24641191, Length=525, Percent_Identity=44.3809523809524, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=43.4285714285714, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=43.4285714285714, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=43.4285714285714, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI17864606, Length=524, Percent_Identity=38.7404580152672, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24584129, Length=528, Percent_Identity=32.3863636363636, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI19921262, Length=528, Percent_Identity=32.3863636363636, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24652903, Length=543, Percent_Identity=22.4677716390424, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17647245, Length=544, Percent_Identity=22.6102941176471, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645179, Length=468, Percent_Identity=22.6495726495727, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583944, Length=131, Percent_Identity=29.0076335877863, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH601_MYCLE (P37578)

Other databases:

- EMBL:   Z11665
- EMBL:   U00015
- EMBL:   U00020
- EMBL:   AL583918
- PIR:   S25181
- RefSeq:   NP_301373.1
- ProteinModelPortal:   P37578
- SMR:   P37578
- EnsemblBacteria:   EBMYCT00000029292
- GeneID:   909064
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0381
- NMPDR:   fig|272631.1.peg.245
- Leproma:   ML0381
- GeneTree:   EBGT00050000016766
- HOGENOM:   HBG625289
- OMA:   WIASNAG
- ProtClustDB:   PRK12849
- BioCyc:   MLEP272631:ML0381-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 55817; Mature: 55686

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIEYDETARHAMEVGMNKLADTVRVTLGPRGRHVVLAKAFGGPTITNDGVTVAREID
CCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCEECCCCCEEEEECC
LEDPFENLGAQLVKSVATKTNDVAGDGTTTATVLAQALVKGGLRMVAAGANPVALGAGIS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCHH
KAADAVSEALLAVATPVAGKDAITQVATVSSRDEQIGALVGEGMNKVGTDGVVSVEESST
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCCEEEECCCCC
LDTELEFTEGVGFDKGFLSAYFVTDFDSQQAVLDDPLVLLHQEKISSLPELLPMLEKVTE
CCCCEEHHCCCCCCCHHHEEEEEECCCCCCHHHCCCHHEEEHHHHHHHHHHHHHHHHHHC
SGKPLLIVAEDLEGEALATLVVNSIRKTLKAVAVKSPFFGDRRKAFLEDLAIVTGGQVVN
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEC
PETGLVLREVGTDVLGSARRVVVSKDDTIIVDGGGSNDAVAKRVNQLRAEIEVSDSEWDR
CCCCCHHHHHCCHHHCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCH
EKLQERVAKLAGGVAVIKVGAVTETALKKRKESVEDAVAAAKASIEEGIIAGGGSALVQC
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHH
GAALKQLRTSLTGDEALGIDVFFEALKAPLYWIATNAGLDGAVVVDKVSGLPAGHGLNAS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCC
TLGYGDLVADGVVDPVKVTRSAVLNAASVARMMLTTETAVVDKPAKTEEHDHHGHAH
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLIEYDETARHAMEVGMNKLADTVRVTLGPRGRHVVLAKAFGGPTITNDGVTVAREID
CCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCEECCCCCEEEEECC
LEDPFENLGAQLVKSVATKTNDVAGDGTTTATVLAQALVKGGLRMVAAGANPVALGAGIS
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCHH
KAADAVSEALLAVATPVAGKDAITQVATVSSRDEQIGALVGEGMNKVGTDGVVSVEESST
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHCCCCCEEEECCCCC
LDTELEFTEGVGFDKGFLSAYFVTDFDSQQAVLDDPLVLLHQEKISSLPELLPMLEKVTE
CCCCEEHHCCCCCCCHHHEEEEEECCCCCCHHHCCCHHEEEHHHHHHHHHHHHHHHHHHC
SGKPLLIVAEDLEGEALATLVVNSIRKTLKAVAVKSPFFGDRRKAFLEDLAIVTGGQVVN
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEC
PETGLVLREVGTDVLGSARRVVVSKDDTIIVDGGGSNDAVAKRVNQLRAEIEVSDSEWDR
CCCCCHHHHHCCHHHCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCH
EKLQERVAKLAGGVAVIKVGAVTETALKKRKESVEDAVAAAKASIEEGIIAGGGSALVQC
HHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHH
GAALKQLRTSLTGDEALGIDVFFEALKAPLYWIATNAGLDGAVVVDKVSGLPAGHGLNAS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCC
TLGYGDLVADGVVDPVKVTRSAVLNAASVARMMLTTETAVVDKPAKTEEHDHHGHAH
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1354834; 11234002