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The map label for this gene is fba

Identifier: 221229541

GI number: 221229541

Start: 367295

End: 368332

Strand: Reverse

Name: fba

Synonym: MLBr_00286

Alternate gene names: 221229541

Gene position: 368332-367295 (Counterclockwise)

Preceding gene: 221229543

Following gene: 221229539

Centisome position: 11.27

GC content: 56.65

Gene sequence:

>1038_bases
ATGCCTATCGCAACACCGGAGATCTACGCCGAGATGTTGAGACGCGCCAAGGAAAACTCATACGCTTTTCCGGCCATCAA
CTGTACGTCTTCAGAGACCGTCAACGCCGCTATCAAAGGCTTTGCTGACGCTGGCAGTGACGGCATCATCCAGTTTTCCA
CCGGTGGTGCGGAGTTCGCCTCCGGGCTCGGCGTGAAGGACATGGTGACGGGTGCGGTAGCGCTAGCGAAGTTCACTCAC
ACTATTGCGGCCAAGTACCCGATCAACGTTGCACTGCACACTGACCACTGTCCCAAGGACAAACTGGACAGTTACGTGCG
CCCTTTGCTGGCGATCTCGGCTAGAAGGGTAGCGACCGGTAAGGATCCGCTGTTCGGATCGCACATGTGGGATGGCTCTG
CGATACCGATCGATGAGAATCTGGCCATAGCCCAAGATCTGCTGAAGGACGCAGCGGCCGCCAAGATCATTCTCGAAGTC
GAGATCGGTGTGGTCGGCGGCGAGGAAGACGGCGTGGCCGGCGAAATCAACGAAAAATTATATACTACTCCGAAGGATTT
CGTGAAGACGATAGATGCTCTGGGCGCCGGTGAACACGGCAAGTATCTGCTGGCTGCGACGTTTGGCAACGTGCACGGTG
TCTATAAGCCTGGCAACGTTAAGCTGCGTCCCGACATCCTGGCCGAAGGTCAGAAGGTGGCAGCGGCCAAGCTCAGTCAG
TCTGAGGGCTCGAAACCGTTCGATTTTGTGTTCCACGGCGGTTCCGGTTCGGAAAAGTCGGAGATCGAGGAGGCGCTGCG
GTACGGTGTGGTGAAGATGAACGTTGACACCGATACCCAATATGCATTCACTCGCCCAGTGTCCGGTCACATGTTCACTA
ACTACGACGGCGTGCTTAAGGTCGACGGCGATGTGGGCAATAAGAAGGTGTACGACCCGCGCAGCTATCTTAAGAAGGCC
GAAGCATCGATGACCGAGCGGGTGCTTGAGGCCTGTAACGATCTACGTTGCGCCGGAAAGTCGGTGGCGGCTAGTTGA

Upstream 100 bases:

>100_bases
CTCTGCTGGCTGTGGACAAAAAATCCTGCGCAGATTGGCTGCTAACCGGCATTGGGATGTTGCGATACTGGATAGACTCA
GTTTTCAGGAGGGAAGTTTC

Downstream 100 bases:

>100_bases
CAGTTGACTGTTGAGTTAGCCGCTAGGGGACTGGCAGATCTTCCATTGATCGTCGCAGAACTGCAGGTCAAGGCTGCGTG
TCGAGCGGACCTGTGGGTCG

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MPIATPEIYAEMLRRAKENSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFASGLGVKDMVTGAVALAKFTH
TIAAKYPINVALHTDHCPKDKLDSYVRPLLAISARRVATGKDPLFGSHMWDGSAIPIDENLAIAQDLLKDAAAAKIILEV
EIGVVGGEEDGVAGEINEKLYTTPKDFVKTIDALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAEGQKVAAAKLSQ
SEGSKPFDFVFHGGSGSEKSEIEEALRYGVVKMNVDTDTQYAFTRPVSGHMFTNYDGVLKVDGDVGNKKVYDPRSYLKKA
EASMTERVLEACNDLRCAGKSVAAS

Sequences:

>Translated_345_residues
MPIATPEIYAEMLRRAKENSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFASGLGVKDMVTGAVALAKFTH
TIAAKYPINVALHTDHCPKDKLDSYVRPLLAISARRVATGKDPLFGSHMWDGSAIPIDENLAIAQDLLKDAAAAKIILEV
EIGVVGGEEDGVAGEINEKLYTTPKDFVKTIDALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAEGQKVAAAKLSQ
SEGSKPFDFVFHGGSGSEKSEIEEALRYGVVKMNVDTDTQYAFTRPVSGHMFTNYDGVLKVDGDVGNKKVYDPRSYLKKA
EASMTERVLEACNDLRCAGKSVAAS
>Mature_344_residues
PIATPEIYAEMLRRAKENSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFASGLGVKDMVTGAVALAKFTHT
IAAKYPINVALHTDHCPKDKLDSYVRPLLAISARRVATGKDPLFGSHMWDGSAIPIDENLAIAQDLLKDAAAAKIILEVE
IGVVGGEEDGVAGEINEKLYTTPKDFVKTIDALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAEGQKVAAAKLSQS
EGSKPFDFVFHGGSGSEKSEIEEALRYGVVKMNVDTDTQYAFTRPVSGHMFTNYDGVLKVDGDVGNKKVYDPRSYLKKAE
ASMTERVLEACNDLRCAGKSVAAS

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789293, Length=342, Percent_Identity=39.766081871345, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082041, Length=331, Percent_Identity=24.773413897281, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789526, Length=322, Percent_Identity=24.5341614906832, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322790, Length=337, Percent_Identity=37.6854599406528, Blast_Score=202, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_MYCLE (O69600)

Other databases:

- EMBL:   AL023514
- EMBL:   AL583918
- PIR:   F86944
- RefSeq:   NP_301326.1
- ProteinModelPortal:   O69600
- SMR:   O69600
- EnsemblBacteria:   EBMYCT00000029368
- GeneID:   908824
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0286
- NMPDR:   fig|272631.1.peg.198
- Leproma:   ML0286
- GeneTree:   EBGT00050000016874
- HOGENOM:   HBG327581
- OMA:   EEDGVSH
- ProtClustDB:   PRK09197
- BioCyc:   MLEP272631:ML0286-MONOMER
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01520

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 36741; Mature: 36610

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 95-95 BINDING 53-53 BINDING 213-213

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIATPEIYAEMLRRAKENSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFA
CCCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHH
SGLGVKDMVTGAVALAKFTHTIAAKYPINVALHTDHCPKDKLDSYVRPLLAISARRVATG
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KDPLFGSHMWDGSAIPIDENLAIAQDLLKDAAAAKIILEVEIGVVGGEEDGVAGEINEKL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCHH
YTTPKDFVKTIDALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAEGQKVAAAKLSQ
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCCEEECCHHHHCCCHHHHHHHHH
SEGSKPFDFVFHGGSGSEKSEIEEALRYGVVKMNVDTDTQYAFTRPVSGHMFTNYDGVLK
CCCCCCEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCEEECCCCEEE
VDGDVGNKKVYDPRSYLKKAEASMTERVLEACNDLRCAGKSVAAS
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
PIATPEIYAEMLRRAKENSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFA
CCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHH
SGLGVKDMVTGAVALAKFTHTIAAKYPINVALHTDHCPKDKLDSYVRPLLAISARRVATG
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KDPLFGSHMWDGSAIPIDENLAIAQDLLKDAAAAKIILEVEIGVVGGEEDGVAGEINEKL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCCHH
YTTPKDFVKTIDALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAEGQKVAAAKLSQ
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCCEEECCHHHHCCCHHHHHHHHH
SEGSKPFDFVFHGGSGSEKSEIEEALRYGVVKMNVDTDTQYAFTRPVSGHMFTNYDGVLK
CCCCCCEEEEEECCCCCCHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCEEECCCCEEE
VDGDVGNKKVYDPRSYLKKAEASMTERVLEACNDLRCAGKSVAAS
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002