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The map label for this gene is metZ [H]

Identifier: 221229533

GI number: 221229533

Start: 357573

End: 358793

Strand: Reverse

Name: metZ [H]

Synonym: MLBr_00275

Alternate gene names: 221229533

Gene position: 358793-357573 (Counterclockwise)

Preceding gene: 221229534

Following gene: 221229532

Centisome position: 10.98

GC content: 58.15

Gene sequence:

>1221_bases
ATGACCGACGACCGCTCTGTTCGCACGCCTAAGGCTTTGCCGGACGGCGTCACCGCGGCAACCGTGGGCGTCCGTGGTGG
CCTGTTACGGTCCCGGTTCGAAGAAACCGCCGAGACGATGTACTTGACGTCTGGTTACGTCTACGAGTCAGCGGTCGTTG
CAGAAAAGTCGTTCACCGGCGAGCTGGACCACTTTGTGTATTCGCGTTACGGCAACCCTACCGTGACGATGTTCGAGGAA
CGGTTACGCCTGATCGAGGGAGCCCCTGCGGCGTTCGTCACCGCGAGCGGCATGGCTGCGGTTTTCACTTCGCTAGGTGC
GTTGTTGGCCTCCGGCGACCGGTTGGTGGCCGCGCGCAGTCTGTTTGGGTCATGTTTCGTGGTGTGCAATGAAATTCTAC
CGCGGTGGGGTGTCGAGACCGTCTTCGTCGACGGCGAAGACCTTGTGCAATGGGAGCAGGCGCTTTCGGTTCCAACTCAA
GCGGTGTTCTTTGAGACGCCCTCGAATCCGATGCAGTCGCTGGTAGATATCGCGGCCGTGACTGAGTTGGCTCACGCGGC
TGGAGCAAAAGTTGTTCTGGACAATGCCTTTGCTACACCACTACTGCAGCACGGTTTACCGCTCGGGGTTGACGTGGTAA
TCTATTCAGGTACTAAACACATCGACGGCCAGGGGCGGGTGTTGGGCGGTGCCATTCTGGGTGACAGGGATTACATCGAC
GGTCCTGTGCAAAAGCTGATGCGCCACACTGGCCCAGCAATGAGCGCTTTCAATGCCTGGATATTGCTGAAAGGTCTTGA
GACACTTGCGATTAGGGTCGATCACTGTAATTCCTCGGCGCACCGTATTGCAGAGTTCTTGGAGAAGCACCCGGCGGTGA
GTTGGGTGCGCTATCCATTTTTGGTGTCGCATCCGCAGTACGACCTGGCGAAGCGCCAAATGTCTGGTGGTGGAACGGTG
GTGACCTTTGCGCTCAATTCTCCGGAGGACGCCGCTAAGCAACGAGCCTTCGAAGTATTGGACAGGCTGCGGCTGATCGA
TATTTCTAACAACTTCGGTGACGTTAAATCGCTTATTACGCACCCGGCGACTACCACTCACCGCGCGATGGGCCCGGAGG
GCCGTGCTGCGATCGGGCTCGGTGACGGTGTGGTACGCATCTCCATTGGCCTCGAAGACGCCGCCGATCTGATCGCTGAT
ATTGATCAGGCCCTGAGCTAG

Upstream 100 bases:

>100_bases
AAACCGGTTGGCGTGCGGTTGGGTTGCCGTGGAAGCAGCTGTGACTCGCGCCAGTGATGAGGTGGGGGCAGCTTCCGCGT
GCGTTGGAGACCCGGCGCAA

Downstream 100 bases:

>100_bases
TAGCGCTAGCCGGATGCAGTGCGCGATTGCCGAGCAGCGGTGTAGTTTGTACGTATTCAGATTGCATCTGCAGTAGGAGA
TGGCGATGAAAACCGCGGCG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTDDRSVRTPKALPDGVTAATVGVRGGLLRSRFEETAETMYLTSGYVYESAVVAEKSFTGELDHFVYSRYGNPTVTMFEE
RLRLIEGAPAAFVTASGMAAVFTSLGALLASGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLVQWEQALSVPTQ
AVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNAFATPLLQHGLPLGVDVVIYSGTKHIDGQGRVLGGAILGDRDYID
GPVQKLMRHTGPAMSAFNAWILLKGLETLAIRVDHCNSSAHRIAEFLEKHPAVSWVRYPFLVSHPQYDLAKRQMSGGGTV
VTFALNSPEDAAKQRAFEVLDRLRLIDISNNFGDVKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISIGLEDAADLIAD
IDQALS

Sequences:

>Translated_406_residues
MTDDRSVRTPKALPDGVTAATVGVRGGLLRSRFEETAETMYLTSGYVYESAVVAEKSFTGELDHFVYSRYGNPTVTMFEE
RLRLIEGAPAAFVTASGMAAVFTSLGALLASGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLVQWEQALSVPTQ
AVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNAFATPLLQHGLPLGVDVVIYSGTKHIDGQGRVLGGAILGDRDYID
GPVQKLMRHTGPAMSAFNAWILLKGLETLAIRVDHCNSSAHRIAEFLEKHPAVSWVRYPFLVSHPQYDLAKRQMSGGGTV
VTFALNSPEDAAKQRAFEVLDRLRLIDISNNFGDVKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISIGLEDAADLIAD
IDQALS
>Mature_405_residues
TDDRSVRTPKALPDGVTAATVGVRGGLLRSRFEETAETMYLTSGYVYESAVVAEKSFTGELDHFVYSRYGNPTVTMFEER
LRLIEGAPAAFVTASGMAAVFTSLGALLASGDRLVAARSLFGSCFVVCNEILPRWGVETVFVDGEDLVQWEQALSVPTQA
VFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNAFATPLLQHGLPLGVDVVIYSGTKHIDGQGRVLGGAILGDRDYIDG
PVQKLMRHTGPAMSAFNAWILLKGLETLAIRVDHCNSSAHRIAEFLEKHPAVSWVRYPFLVSHPQYDLAKRQMSGGGTVV
TFALNSPEDAAKQRAFEVLDRLRLIDISNNFGDVKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISIGLEDAADLIADI
DQALS

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=347, Percent_Identity=33.4293948126801, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI299473759, Length=347, Percent_Identity=30.5475504322767, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI25453487, Length=346, Percent_Identity=29.1907514450867, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790375, Length=389, Percent_Identity=33.4190231362468, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789383, Length=350, Percent_Identity=28, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560056, Length=350, Percent_Identity=35.4285714285714, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=350, Percent_Identity=35.1428571428571, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71980708, Length=347, Percent_Identity=32.5648414985591, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=417, Percent_Identity=31.1750599520384, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=350, Percent_Identity=34.8571428571429, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=365, Percent_Identity=27.3972602739726, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=287, Percent_Identity=26.4808362369338, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=344, Percent_Identity=24.7093023255814, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=348, Percent_Identity=32.4712643678161, Blast_Score=195, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43642; Mature: 43511

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDRSVRTPKALPDGVTAATVGVRGGLLRSRFEETAETMYLTSGYVYESAVVAEKSFTG
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCEEHHHHHHCCCCCC
ELDHFVYSRYGNPTVTMFEERLRLIEGAPAAFVTASGMAAVFTSLGALLASGDRLVAARS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHH
LFGSCFVVCNEILPRWGVETVFVDGEDLVQWEQALSVPTQAVFFETPSNPMQSLVDIAAV
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
TELAHAAGAKVVLDNAFATPLLQHGLPLGVDVVIYSGTKHIDGQGRVLGGAILGDRDYID
HHHHHHCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCCCCCC
GPVQKLMRHTGPAMSAFNAWILLKGLETLAIRVDHCNSSAHRIAEFLEKHPAVSWVRYPF
HHHHHHHHHCCHHHHHHHHHHHEECHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCE
LVSHPQYDLAKRQMSGGGTVVTFALNSPEDAAKQRAFEVLDRLRLIDISNNFGDVKSLIT
EECCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
HPATTTHRAMGPEGRAAIGLGDGVVRISIGLEDAADLIADIDQALS
CCCCCCHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDDRSVRTPKALPDGVTAATVGVRGGLLRSRFEETAETMYLTSGYVYESAVVAEKSFTG
CCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCEEHHHHHHCCCCCC
ELDHFVYSRYGNPTVTMFEERLRLIEGAPAAFVTASGMAAVFTSLGALLASGDRLVAARS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHH
LFGSCFVVCNEILPRWGVETVFVDGEDLVQWEQALSVPTQAVFFETPSNPMQSLVDIAAV
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
TELAHAAGAKVVLDNAFATPLLQHGLPLGVDVVIYSGTKHIDGQGRVLGGAILGDRDYID
HHHHHHCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCCCCCC
GPVQKLMRHTGPAMSAFNAWILLKGLETLAIRVDHCNSSAHRIAEFLEKHPAVSWVRYPF
HHHHHHHHHCCHHHHHHHHHHHEECHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCE
LVSHPQYDLAKRQMSGGGTVVTFALNSPEDAAKQRAFEVLDRLRLIDISNNFGDVKSLIT
EECCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHH
HPATTTHRAMGPEGRAAIGLGDGVVRISIGLEDAADLIADIDQALS
CCCCCCHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]