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The map label for this gene is eno [H]

Identifier: 221229525

GI number: 221229525

Start: 333767

End: 335110

Strand: Direct

Name: eno [H]

Synonym: MLBr_00255

Alternate gene names: 221229525

Gene position: 333767-335110 (Clockwise)

Preceding gene: 221229524

Following gene: 221229526

Centisome position: 10.21

GC content: 60.27

Gene sequence:

>1344_bases
TTGAGCCCACCCACAGGACGTCCACGTCTCAGCCATGACTATAAGGAGAACTTGGTGCCGGTTATCGAGCAGGTCGGGGC
CCGCGAGATCCTCGATTCCCGCGGCAACCCCACGGTCGAAGTCGAGGTGGTGCTGATTGACGGAACCTTCGCCCGCGCAG
CGGTGCCTTCGGGCGCATCGACCGGCGAGTATGAGGCCGTCGAGTTACGTGACGGCGACGGTCGTTACGGTGGCAAGGGT
GTGAAAAGGGCCGTGGATGCTGTGCTCGACGAGATCGGTCCAGTGGTGATCGGCCTCAACGCCAATGACCAGCGGCTGAT
CGATCAGGAGCTGCTGGACCTCGACGGCACCCCCGACAAGTCCCGATTGGGTGGTAACGCGATCCTGGGTGTCTCGCTGG
CCGTCGCCAAGGCAGCCGCCGATTCAGCGGAATTGCCGCTGTTCCGTTATATTGGAGGGTCTAACGCACATATCCTTCCG
GTGCCTATGATGAACATTCTCAACGGCGGCGCGCACGCCGACACTGCTGTTGATGTGCAGGAGTTCATGGTGGCACCGAT
CGGGGCACCCAGTTTCGTTGAGGCGCTGCGTTGGGGCGCCGAGGTATATCACGCTCTCAAATCGGTTCTGAAGAAAAAAG
GGTTAAGCACCGGCCTGGGTGATGAGGGTGGCTTTGCCCCTGAAGTGGCCGGAACCACCGCGGCGCTGGACTTGGTCACC
TTAGCTATTGAAGCGGCGGGTTTTAAGCCGGGTGCCGATGTGGCATTGGCCCTTGATGCGGCAGCCACCGAGTTCTACAC
GGACGGCATCGGCTACCATTTCGAGGGCATGACCCACACTGCCGACCAGATGACTGAGTTTTATGCCGACTTGCTGGGTT
CATACCCACTGGTGTCCATCGAAGATCCGCTGTCCGAAGATGATTGGGACGGTTGGGCTGCTCTGACGGCGTCGATAGGG
GAACAGGTGCAGATCGTCGGGGACGACATCTTTGCCACCAATCCTGAGAGACTGGAGGAGGGCATCGGTCGCGGTGTGGC
AAACGCGTTGCTGGTCAAGGTGAACCAGATAGGCACGTTGACCGAGACGCTCGAAGCTGTTGCGTTGGCACATCACAGCG
GATATCGCACGATGATCAGCCACCGCAGTGGCGAGACCGAGGACACGATGATCGCTGACCTGGTCGTGGCTCTCGGTAGC
GGGCAAATTAAGACGGGAGCGCCCGCCCGCAGTGAGCGCGTCGCTAAATATAACCAGCTGCTGCGGATTGAAGAGGAGCT
CGGCGACGCCGCCCGCTATGCCGGCGATCTGGCATTTCTGCGATACGTCGTGGAAACGAGATAA

Upstream 100 bases:

>100_bases
CTGGTCACCCGCTGTAGAAAAATAGATTTAGGGTGATCCAGGCTTTACGCTAAAGGCGGCCTGGGACCGACGCGCCAGTG
GAGGCTCTTCGCTGGCGGGG

Downstream 100 bases:

>100_bases
GTGACAGCCAATCAGGAACACTGCTAGATTTCCCATGTCCGAAGCGAAACGGCTAGATCCGAAGCGCCGCTCCCCGGCAT
CGCGTCCGGGGAAAGCCGGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSPPTGRPRLSHDYKENLVPVIEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGASTGEYEAVELRDGDGRYGGKG
VKRAVDAVLDEIGPVVIGLNANDQRLIDQELLDLDGTPDKSRLGGNAILGVSLAVAKAAADSAELPLFRYIGGSNAHILP
VPMMNILNGGAHADTAVDVQEFMVAPIGAPSFVEALRWGAEVYHALKSVLKKKGLSTGLGDEGGFAPEVAGTTAALDLVT
LAIEAAGFKPGADVALALDAAATEFYTDGIGYHFEGMTHTADQMTEFYADLLGSYPLVSIEDPLSEDDWDGWAALTASIG
EQVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTLTETLEAVALAHHSGYRTMISHRSGETEDTMIADLVVALGS
GQIKTGAPARSERVAKYNQLLRIEEELGDAARYAGDLAFLRYVVETR

Sequences:

>Translated_447_residues
MSPPTGRPRLSHDYKENLVPVIEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGASTGEYEAVELRDGDGRYGGKG
VKRAVDAVLDEIGPVVIGLNANDQRLIDQELLDLDGTPDKSRLGGNAILGVSLAVAKAAADSAELPLFRYIGGSNAHILP
VPMMNILNGGAHADTAVDVQEFMVAPIGAPSFVEALRWGAEVYHALKSVLKKKGLSTGLGDEGGFAPEVAGTTAALDLVT
LAIEAAGFKPGADVALALDAAATEFYTDGIGYHFEGMTHTADQMTEFYADLLGSYPLVSIEDPLSEDDWDGWAALTASIG
EQVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTLTETLEAVALAHHSGYRTMISHRSGETEDTMIADLVVALGS
GQIKTGAPARSERVAKYNQLLRIEEELGDAARYAGDLAFLRYVVETR
>Mature_446_residues
SPPTGRPRLSHDYKENLVPVIEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGASTGEYEAVELRDGDGRYGGKGV
KRAVDAVLDEIGPVVIGLNANDQRLIDQELLDLDGTPDKSRLGGNAILGVSLAVAKAAADSAELPLFRYIGGSNAHILPV
PMMNILNGGAHADTAVDVQEFMVAPIGAPSFVEALRWGAEVYHALKSVLKKKGLSTGLGDEGGFAPEVAGTTAALDLVTL
AIEAAGFKPGADVALALDAAATEFYTDGIGYHFEGMTHTADQMTEFYADLLGSYPLVSIEDPLSEDDWDGWAALTASIGE
QVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTLTETLEAVALAHHSGYRTMISHRSGETEDTMIADLVVALGSG
QIKTGAPARSERVAKYNQLLRIEEELGDAARYAGDLAFLRYVVETR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=55.3738317757009, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=52.2144522144522, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=52.2144522144522, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=51.7482517482517, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=48.711943793911, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=338, Percent_Identity=25.7396449704142, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI169201757, Length=338, Percent_Identity=25.7396449704142, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI239744207, Length=338, Percent_Identity=25.7396449704142, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1789141, Length=422, Percent_Identity=54.2654028436019, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=51.3953488372093, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=51.3953488372093, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=51.5789473684211, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=51.270207852194, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.6944444444444, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.6944444444444, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.6944444444444, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.5773672055427, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50.6976744186047, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50.6976744186047, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50.6976744186047, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50.6976744186047, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50.6976744186047, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50.6976744186047, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47251; Mature: 47119

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPTGRPRLSHDYKENLVPVIEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGAS
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCC
TGEYEAVELRDGDGRYGGKGVKRAVDAVLDEIGPVVIGLNANDQRLIDQELLDLDGTPDK
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCH
SRLGGNAILGVSLAVAKAAADSAELPLFRYIGGSNAHILPVPMMNILNGGAHADTAVDVQ
HHCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCHHHHHCCCCCCCHHHHHH
EFMVAPIGAPSFVEALRWGAEVYHALKSVLKKKGLSTGLGDEGGFAPEVAGTTAALDLVT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LAIEAAGFKPGADVALALDAAATEFYTDGIGYHFEGMTHTADQMTEFYADLLGSYPLVSI
HHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEE
EDPLSEDDWDGWAALTASIGEQVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTL
CCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
TETLEAVALAHHSGYRTMISHRSGETEDTMIADLVVALGSGQIKTGAPARSERVAKYNQL
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LRIEEELGDAARYAGDLAFLRYVVETR
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPPTGRPRLSHDYKENLVPVIEQVGAREILDSRGNPTVEVEVVLIDGTFARAAVPSGAS
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEEECCHHHHCCCCCCC
TGEYEAVELRDGDGRYGGKGVKRAVDAVLDEIGPVVIGLNANDQRLIDQELLDLDGTPDK
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCH
SRLGGNAILGVSLAVAKAAADSAELPLFRYIGGSNAHILPVPMMNILNGGAHADTAVDVQ
HHCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCHHHHHCCCCCCCHHHHHH
EFMVAPIGAPSFVEALRWGAEVYHALKSVLKKKGLSTGLGDEGGFAPEVAGTTAALDLVT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LAIEAAGFKPGADVALALDAAATEFYTDGIGYHFEGMTHTADQMTEFYADLLGSYPLVSI
HHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEE
EDPLSEDDWDGWAALTASIGEQVQIVGDDIFATNPERLEEGIGRGVANALLVKVNQIGTL
CCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
TETLEAVALAHHSGYRTMISHRSGETEDTMIADLVVALGSGQIKTGAPARSERVAKYNQL
HHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LRIEEELGDAARYAGDLAFLRYVVETR
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]