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The map label for this gene is folK

Identifier: 221229501

GI number: 221229501

Start: 297956

End: 298531

Strand: Direct

Name: folK

Synonym: MLBr_00226

Alternate gene names: 221229501

Gene position: 297956-298531 (Clockwise)

Preceding gene: 221229500

Following gene: 221229502

Centisome position: 9.12

GC content: 63.54

Gene sequence:

>576_bases
ATGACTCGCGTCGTACTGTCTATAGGATCCAATCTGGGTGACCGGCTGGCGTGGCTGCAGTCAGCCGTCGATGGGCTGGG
TGATGCGGTTGTCGCGGTCTCTCCGGTGTATGACACCGTCCCGTGGGGAGCCGTGGAGCAGAGATCGTTCCTCAACGCGG
TAGTGATCGCCGACGGCCCGGCGTATGACACGAAGGCCTGGCTGTGTCGGGCTCAGGAGCTCGAGCGGAACGCGGGTCGG
GTGCGGGGACAGCGCTGGGGCGCACGCACACTCGACGTCGATCTGATCTCCTGCTATCAGACTTCGGGTGCTACGACCGG
TGCGGTCGAGGTGATTACGTGCGAAAGCAACTTGACGTTGCCGCATCCGCGGGCTCATCTGCGGGCTTTCGTGCTGGTTC
CGTGGTTGGCTGTCGACTCGGATGCTGAGCTGACCGTCGCTGGGCGGGCGCAACGGGTCGATCGGCTGTTGGCAGAGATG
GAGCCGACCGAGCGGGAAGGTGTGCGGTTGACCAATCTGACGCTCAAGCTGAAACGTAGTTCGCCGGCGCGGCCAGTCAG
TCCGAAATCAGACTGA

Upstream 100 bases:

>100_bases
CGCAGGCGCCGATCCCGCAGCAGTTCTCTGATGTGGCGGTGGTGGTTCGGCGATCGCGACGCGGCGGTCGCGGTTCGATG
ATTCCTGCGAGCGGGGTGTG

Downstream 100 bases:

>100_bases
TGGGACCGACCCGGAAACGTGACCTGACGGCCGCGATGATTGGCGCTGCGGTAGTGGGTTATCTGCTGGTGCTTGTGCTG
TACCGGTGGTTTCCACCCAT

Product: putative 2-amino-4-hydroxy-6-hydroxymethyldihydropterine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTRVVLSIGSNLGDRLAWLQSAVDGLGDAVVAVSPVYDTVPWGAVEQRSFLNAVVIADGPAYDTKAWLCRAQELERNAGR
VRGQRWGARTLDVDLISCYQTSGATTGAVEVITCESNLTLPHPRAHLRAFVLVPWLAVDSDAELTVAGRAQRVDRLLAEM
EPTEREGVRLTNLTLKLKRSSPARPVSPKSD

Sequences:

>Translated_191_residues
MTRVVLSIGSNLGDRLAWLQSAVDGLGDAVVAVSPVYDTVPWGAVEQRSFLNAVVIADGPAYDTKAWLCRAQELERNAGR
VRGQRWGARTLDVDLISCYQTSGATTGAVEVITCESNLTLPHPRAHLRAFVLVPWLAVDSDAELTVAGRAQRVDRLLAEM
EPTEREGVRLTNLTLKLKRSSPARPVSPKSD
>Mature_190_residues
TRVVLSIGSNLGDRLAWLQSAVDGLGDAVVAVSPVYDTVPWGAVEQRSFLNAVVIADGPAYDTKAWLCRAQELERNAGRV
RGQRWGARTLDVDLISCYQTSGATTGAVEVITCESNLTLPHPRAHLRAFVLVPWLAVDSDAELTVAGRAQRVDRLLAEME
PTEREGVRLTNLTLKLKRSSPARPVSPKSD

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family

Homologues:

Organism=Escherichia coli, GI1786335, Length=143, Percent_Identity=34.965034965035, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HPPK_MYCLE (O69528)

Other databases:

- EMBL:   AL023093
- EMBL:   AL583917
- PIR:   B86937
- RefSeq:   NP_301286.1
- ProteinModelPortal:   O69528
- SMR:   O69528
- EnsemblBacteria:   EBMYCT00000028313
- GeneID:   908689
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0226
- NMPDR:   fig|272631.1.peg.158
- Leproma:   ML0226
- GeneTree:   EBGT00050000016430
- HOGENOM:   HBG662565
- OMA:   ETDPVGY
- ProtClustDB:   CLSK2460421
- BioCyc:   MLEP272631:ML0226-MONOMER
- BRENDA:   2.7.6.3
- InterPro:   IPR000550
- Gene3D:   G3DSA:3.30.70.560
- TIGRFAMs:   TIGR01498

Pfam domain/function: PF01288 HPPK; SSF55083 Hppk

EC number: =2.7.6.3

Molecular weight: Translated: 20761; Mature: 20630

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVVLSIGSNLGDRLAWLQSAVDGLGDAVVAVSPVYDTVPWGAVEQRSFLNAVVIADGP
CEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHEEEEEEECCC
AYDTKAWLCRAQELERNAGRVRGQRWGARTLDVDLISCYQTSGATTGAVEVITCESNLTL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHEEEEECCCCCCCCEEEEEEECCCCCC
PHPRAHLRAFVLVPWLAVDSDAELTVAGRAQRVDRLLAEMEPTEREGVRLTNLTLKLKRS
CCCHHHHEEEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC
SPARPVSPKSD
CCCCCCCCCCC
>Mature Secondary Structure 
TRVVLSIGSNLGDRLAWLQSAVDGLGDAVVAVSPVYDTVPWGAVEQRSFLNAVVIADGP
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHEEEEEEECCC
AYDTKAWLCRAQELERNAGRVRGQRWGARTLDVDLISCYQTSGATTGAVEVITCESNLTL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECHHEEEEECCCCCCCCEEEEEEECCCCCC
PHPRAHLRAFVLVPWLAVDSDAELTVAGRAQRVDRLLAEMEPTEREGVRLTNLTLKLKRS
CCCHHHHEEEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC
SPARPVSPKSD
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002