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The map label for this gene is ppa [H]

Identifier: 221229491

GI number: 221229491

Start: 281558

End: 282046

Strand: Reverse

Name: ppa [H]

Synonym: MLBr_00210

Alternate gene names: 221229491

Gene position: 282046-281558 (Counterclockwise)

Preceding gene: 221229496

Following gene: 221229488

Centisome position: 8.63

GC content: 58.08

Gene sequence:

>489_bases
GTGCAATTCGACGTGACCATCGAAATTCCGAAAGGCCAGCGGAACAAGTACGAGGTCGACCACAAAACGGGGCGGGTGCG
TCTAGACCGCTACCTTTACACCCCGATGGCCTATCCGACAGACTACGGCTTCATTGAAGACACCCTCGGCGAAGACGGCG
ACCCGCTGGATGCACTGGTGCTGCTACCGGAGCCGCTGTTCCCTGGCGTACTAGTGGAGGCACGTCCAGTAGGTATGTTC
CGGATGGTCGACGAGCACGGCGGCGACGACAAGGTGTTGTGCGTTCCAGTCAACGACCACCGATGGGATCACATCCACGG
CATCATCGATGTTCCGACCTTCGAGCTGGACGCGATCAAGCATTTCTTCGTACACTACAAGGATCTAGAGCCAGGCAAGT
TCGTGAAAGCCGCAGACTGGGTAGGCCGAGACGAAGCTGAAGCAGAAGTGCAGCGTTCAGTGGAACGGTTCAAGGCTGGC
GGGCACTGA

Upstream 100 bases:

>100_bases
TTGTGCCGAGCCAGCCTTCAGACCAACGTCGTCGGCTAGGGTGAACTCCGCAACGTAACCGATCTACCCGGGCCTAGCTA
AGATTTAAGGAGCCGACACG

Downstream 100 bases:

>100_bases
CACGGATTTCGCGACGAGCTGAAGATCGTTGCTGGAGTGCTCGCCGATGCCCGGTGGCATCCTAGCCACGTGACCCCAAT
GTTGCATTTCGCTGCGACTT

Product: putative inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MQFDVTIEIPKGQRNKYEVDHKTGRVRLDRYLYTPMAYPTDYGFIEDTLGEDGDPLDALVLLPEPLFPGVLVEARPVGMF
RMVDEHGGDDKVLCVPVNDHRWDHIHGIIDVPTFELDAIKHFFVHYKDLEPGKFVKAADWVGRDEAEAEVQRSVERFKAG
GH

Sequences:

>Translated_162_residues
MQFDVTIEIPKGQRNKYEVDHKTGRVRLDRYLYTPMAYPTDYGFIEDTLGEDGDPLDALVLLPEPLFPGVLVEARPVGMF
RMVDEHGGDDKVLCVPVNDHRWDHIHGIIDVPTFELDAIKHFFVHYKDLEPGKFVKAADWVGRDEAEAEVQRSVERFKAG
GH
>Mature_162_residues
MQFDVTIEIPKGQRNKYEVDHKTGRVRLDRYLYTPMAYPTDYGFIEDTLGEDGDPLDALVLLPEPLFPGVLVEARPVGMF
RMVDEHGGDDKVLCVPVNDHRWDHIHGIIDVPTFELDAIKHFFVHYKDLEPGKFVKAADWVGRDEAEAEVQRSVERFKAG
GH

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=157, Percent_Identity=45.859872611465, Blast_Score=136, Evalue=7e-34,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 18533; Mature: 18533

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVTIEIPKGQRNKYEVDHKTGRVRLDRYLYTPMAYPTDYGFIEDTLGEDGDPLDALV
CCEEEEEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCEEEE
LLPEPLFPGVLVEARPVGMFRMVDEHGGDDKVLCVPVNDHRWDHIHGIIDVPTFELDAIK
EECCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHH
HFFVHYKDLEPGKFVKAADWVGRDEAEAEVQRSVERFKAGGH
HHHHEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQFDVTIEIPKGQRNKYEVDHKTGRVRLDRYLYTPMAYPTDYGFIEDTLGEDGDPLDALV
CCEEEEEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCHHHCCCCCCCCEEEE
LLPEPLFPGVLVEARPVGMFRMVDEHGGDDKVLCVPVNDHRWDHIHGIIDVPTFELDAIK
EECCCCCCCEEEECCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHH
HFFVHYKDLEPGKFVKAADWVGRDEAEAEVQRSVERFKAGGH
HHHHEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]