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The map label for this gene is uvrD

Identifier: 221229454

GI number: 221229454

Start: 214299

End: 216635

Strand: Direct

Name: uvrD

Synonym: MLBr_00153

Alternate gene names: 221229454

Gene position: 214299-216635 (Clockwise)

Preceding gene: 221229453

Following gene: 221229456

Centisome position: 6.56

GC content: 57.34

Gene sequence:

>2337_bases
ATGAGTGTGCACACGACAGATGCCAAGCCCGACTCTGAAGTAGATCGGCTGCTTGACGGCCTTAACCCGCAGCAGCGCCA
GGCGGTGGTACATGAGGGTTCGCCGCTGCTGATTGTGGCCGGCGCGGGCTCGGGCAAAACTACAGTATTGGCTCGACGGA
TCGCTTACCTGATTGCAGCCCGAAGTGTCGGGGTCAGTCAGATTCTGGCCATCACCTTCACCAACAAAGCCGCTGCGGAG
ATGCGCGAGCGCGTGGCCCGACTGGTTGGAGACCACACTGGGCCGTCTATGTGGGTGTCGACGTTTCATTCGACCTGCGT
GCGCATTCTACGTAACCAGGCTTCGCTGATCGGCGGGCTCAACTCCAACTTTTCGATCTATGACGTCGATGATTCGCGTA
GCCTGCTGCAGATGATCGGACAAGACATGGGACTCGACATCAAGCGGTATTCGCCACGGCTGATGGCCAACGCCATCTCC
AACTTGAAGAACGAATTGATCGACGCCGAAAGTGCGGTAGCCAACCTGTCTTCAGACACTGATGACCTGGCTCGCACCGT
TGCCACAGTCTACGGCGAATACCAGCGGCGACTGCGGACAGCTAACGCGCTGGACTTCGATGACCTGATCGGAGAAACTG
TTGGGATCCTACAAGCTTTCCCGCAGATCGCCGAACACTACCGGCGGCGGTTCCGGCACGTCCTAGTCGACGAATACCAA
GACACTAACCACGCACAGTACGTGCTGGTGCGGGAATTGGTCGGACACGGGGCCAGGAATTCACCTGACGACATGCCTCC
GGGGGAGTTGTGCGTTGTAGGCGATGCCGACCAGTCGATCTATGCGTTCCGCGGATCCACCATTAGCAATATCGAGGACT
TCGAGCGTGACTACCCGGACACCACAACGATTCTGCTGGAACAGAATTACCGCTCAACGCAAAACATTCTGTCGGCGGCT
AACTCCGTGATCGCTCGTAACTTCGGACGCCGCGACAAGCGCTTGTGGACAGACGCTGGCAAAGGCGAGTTGATCGTTGG
ATACGTCTCCGACAACGAGCACGACGAGGCCAAGTTCATCGCCGATGAAATCGATGCTCTGGTCGGGGGTGGCGAGATTA
CCTACGATGATGTGGCTGTCTTGTATCGAGCTAACAACTTATCGCGATCATTGGAGGAAGTGTTTATCCCCACCGGGATT
CCGTACAAAGTTGTTGGGGGTTTTTGTTTTTATGAAAGCAAGGAGATTCGCGACCTTATCGCTTACCTGCGAGTACTAGA
TAACCCCGGCGATGCCGTCAGTATGCGGCGCATCCTCAACACACCGCGTCGAGGCATCGGCGATCGTGCCGAGGCTTGTG
TGTCGGTGTACGCAGAAAATACCGGAACTAGCTTCGCCGATGCACTACAGGCTGTGGCGGAAGGCAAAGTGCCCATGCTA
AATACCCGCTCGGTGAAAGCGATCGCGGGGTTTGTTGCACTGCTTGACGACCTGCGGTGTCGTGTCGATGACGACCTGGG
TGAACTGGTCGAATCTGTGTTGGAACGCAGCGGATACCTCAGGGAGTTGGAGTCCTCCACGGACCCGCAGGAGTTAGCCC
GTCTGGACAACCTCAATGAACTAGTAAGCTTCGCACACGAATTTAGCACTGAACAGGCTAACGCTGCCGCGCTTGCGAAG
TCCTTGCATACTCCCGAGGATGAAGACGTGCCGGATACCGGTGCGTTGGCGGCGTTTCTGGAAAAGGTGTCACTGATGTC
CGACACCGACCAGATCCCCGAGAACAATTCCGGTGTCGTCACACTAATGACGTTGCATGCCGCGAAAGGATTGGAATTTC
CCGTGGTGTTCGTCACTGGATGGGAGGACGGCATGTTGCCGCACATGCGAACTCTGGGTGATCCGACCGAGCTTTCGGAG
GAGCGTCGGCTGGCCTATGTCGGCATAACCCGGGCACGTCAACGTCTCTACTTGAGCAGGGCCATCACTCGGTCTTCTTG
GGGACAGCCCATTCTCAACCCTGAGTCACGGTTCCTGCGGGAAATTCCTCCGGAACTCATCGACTGGCGGCGTAGTATTC
TCACCGATTCATACAGTACGCCAGCGAGTGGCGCAAGCCGGTTCGGTAGGGTGCGTCCGTCGTCGATCCGGTCCGGGGCG
AGCAAGCGTGCGCTGCTGGTGCTGGCGCCCGGTGATCGAGTGACGCACGACAAGTACGGCCTGGGTCGGGTTGAGGAAGT
TTCCGGGGTCGGTGAATCAGCGATATCACTGATCGACTTCGGCAGTTCGGGGCGAATAAAGCTGATGCACAACCACGCCC
CTGTCACCAAGCTTTGA

Upstream 100 bases:

>100_bases
AGCTTCACGCTCGGTTAGCACGAGCCCCGGCGGGTCAGATCATGCTCGCTGTGTAGCCTGGCCAGCCGCCATAGGCGTAC
GGCGGTAGATTTGAAAGTAC

Downstream 100 bases:

>100_bases
GCCGGCACGGGTCATAACCCCATAAGTCAACGTTTGGTTAGCGCGAGTGTAACGCCCTCATTACGCTGCCGGGCGGCAGC
AGCGGAAGGGCGTGCACAAT

Product: putative ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MSVHTTDAKPDSEVDRLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTTVLARRIAYLIAARSVGVSQILAITFTNKAAAE
MRERVARLVGDHTGPSMWVSTFHSTCVRILRNQASLIGGLNSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAIS
NLKNELIDAESAVANLSSDTDDLARTVATVYGEYQRRLRTANALDFDDLIGETVGILQAFPQIAEHYRRRFRHVLVDEYQ
DTNHAQYVLVRELVGHGARNSPDDMPPGELCVVGDADQSIYAFRGSTISNIEDFERDYPDTTTILLEQNYRSTQNILSAA
NSVIARNFGRRDKRLWTDAGKGELIVGYVSDNEHDEAKFIADEIDALVGGGEITYDDVAVLYRANNLSRSLEEVFIPTGI
PYKVVGGFCFYESKEIRDLIAYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVSVYAENTGTSFADALQAVAEGKVPML
NTRSVKAIAGFVALLDDLRCRVDDDLGELVESVLERSGYLRELESSTDPQELARLDNLNELVSFAHEFSTEQANAAALAK
SLHTPEDEDVPDTGALAAFLEKVSLMSDTDQIPENNSGVVTLMTLHAAKGLEFPVVFVTGWEDGMLPHMRTLGDPTELSE
ERRLAYVGITRARQRLYLSRAITRSSWGQPILNPESRFLREIPPELIDWRRSILTDSYSTPASGASRFGRVRPSSIRSGA
SKRALLVLAPGDRVTHDKYGLGRVEEVSGVGESAISLIDFGSSGRIKLMHNHAPVTKL

Sequences:

>Translated_778_residues
MSVHTTDAKPDSEVDRLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTTVLARRIAYLIAARSVGVSQILAITFTNKAAAE
MRERVARLVGDHTGPSMWVSTFHSTCVRILRNQASLIGGLNSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAIS
NLKNELIDAESAVANLSSDTDDLARTVATVYGEYQRRLRTANALDFDDLIGETVGILQAFPQIAEHYRRRFRHVLVDEYQ
DTNHAQYVLVRELVGHGARNSPDDMPPGELCVVGDADQSIYAFRGSTISNIEDFERDYPDTTTILLEQNYRSTQNILSAA
NSVIARNFGRRDKRLWTDAGKGELIVGYVSDNEHDEAKFIADEIDALVGGGEITYDDVAVLYRANNLSRSLEEVFIPTGI
PYKVVGGFCFYESKEIRDLIAYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVSVYAENTGTSFADALQAVAEGKVPML
NTRSVKAIAGFVALLDDLRCRVDDDLGELVESVLERSGYLRELESSTDPQELARLDNLNELVSFAHEFSTEQANAAALAK
SLHTPEDEDVPDTGALAAFLEKVSLMSDTDQIPENNSGVVTLMTLHAAKGLEFPVVFVTGWEDGMLPHMRTLGDPTELSE
ERRLAYVGITRARQRLYLSRAITRSSWGQPILNPESRFLREIPPELIDWRRSILTDSYSTPASGASRFGRVRPSSIRSGA
SKRALLVLAPGDRVTHDKYGLGRVEEVSGVGESAISLIDFGSSGRIKLMHNHAPVTKL
>Mature_777_residues
SVHTTDAKPDSEVDRLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTTVLARRIAYLIAARSVGVSQILAITFTNKAAAEM
RERVARLVGDHTGPSMWVSTFHSTCVRILRNQASLIGGLNSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAISN
LKNELIDAESAVANLSSDTDDLARTVATVYGEYQRRLRTANALDFDDLIGETVGILQAFPQIAEHYRRRFRHVLVDEYQD
TNHAQYVLVRELVGHGARNSPDDMPPGELCVVGDADQSIYAFRGSTISNIEDFERDYPDTTTILLEQNYRSTQNILSAAN
SVIARNFGRRDKRLWTDAGKGELIVGYVSDNEHDEAKFIADEIDALVGGGEITYDDVAVLYRANNLSRSLEEVFIPTGIP
YKVVGGFCFYESKEIRDLIAYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVSVYAENTGTSFADALQAVAEGKVPMLN
TRSVKAIAGFVALLDDLRCRVDDDLGELVESVLERSGYLRELESSTDPQELARLDNLNELVSFAHEFSTEQANAAALAKS
LHTPEDEDVPDTGALAAFLEKVSLMSDTDQIPENNSGVVTLMTLHAAKGLEFPVVFVTGWEDGMLPHMRTLGDPTELSEE
RRLAYVGITRARQRLYLSRAITRSSWGQPILNPESRFLREIPPELIDWRRSILTDSYSTPASGASRFGRVRPSSIRSGAS
KRALLVLAPGDRVTHDKYGLGRVEEVSGVGESAISLIDFGSSGRIKLMHNHAPVTKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI2367296, Length=754, Percent_Identity=38.4615384615385, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=36.849925705795, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=26.1494252873563, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=764, Percent_Identity=29.0575916230367, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=698, Percent_Identity=23.0659025787966, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): PCRA_MYCLE (Q9CD72)

Other databases:

- EMBL:   AL583917
- PIR:   A86928
- RefSeq:   NP_301239.1
- ProteinModelPortal:   Q9CD72
- SMR:   Q9CD72
- EnsemblBacteria:   EBMYCT00000028978
- GeneID:   908505
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0153
- NMPDR:   fig|272631.1.peg.111
- Leproma:   ML0153
- GeneTree:   EBGT00050000015808
- HOGENOM:   HBG467281
- OMA:   WRGAEVE
- ProtClustDB:   CLSK799345
- BioCyc:   MLEP272631:ML0153-MONOMER
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016
- Gene3D:   G3DSA:1.10.10.160
- PANTHER:   PTHR11070

Pfam domain/function: PF00580 UvrD-helicase

EC number: =3.6.4.12

Molecular weight: Translated: 85636; Mature: 85505

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS51198 UVRD_HELICASE_ATP_BIND; PS51217 UVRD_HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHTTDAKPDSEVDRLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTTVLARRIAYLIAA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
RSVGVSQILAITFTNKAAAEMRERVARLVGDHTGPSMWVSTFHSTCVRILRNQASLIGGL
HHCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAISNLKNELIDAESAVANLSSDT
CCCCEEEECCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DDLARTVATVYGEYQRRLRTANALDFDDLIGETVGILQAFPQIAEHYRRRFRHVLVDEYQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTNHAQYVLVRELVGHGARNSPDDMPPGELCVVGDADQSIYAFRGSTISNIEDFERDYPD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHCCCC
TTTILLEQNYRSTQNILSAANSVIARNFGRRDKRLWTDAGKGELIVGYVSDNEHDEAKFI
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
ADEIDALVGGGEITYDDVAVLYRANNLSRSLEEVFIPTGIPYKVVGGFCFYESKEIRDLI
HHHHHHHHCCCCEEHHHHEEEEECHHHHHHHHHHHCCCCCCHHHHCCCCEECCHHHHHHH
AYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVSVYAENTGTSFADALQAVAEGKVPML
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
NTRSVKAIAGFVALLDDLRCRVDDDLGELVESVLERSGYLRELESSTDPQELARLDNLNE
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCHHH
LVSFAHEFSTEQANAAALAKSLHTPEDEDVPDTGALAAFLEKVSLMSDTDQIPENNSGVV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCCCCCCEE
TLMTLHAAKGLEFPVVFVTGWEDGMLPHMRTLGDPTELSEERRLAYVGITRARQRLYLSR
EEEEEHHCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCEEEEEHHHHHHHHHHHH
AITRSSWGQPILNPESRFLREIPPELIDWRRSILTDSYSTPASGASRFGRVRPSSIRSGA
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHCCC
SKRALLVLAPGDRVTHDKYGLGRVEEVSGVGESAISLIDFGSSGRIKLMHNHAPVTKL
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHEECCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SVHTTDAKPDSEVDRLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTTVLARRIAYLIAA
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
RSVGVSQILAITFTNKAAAEMRERVARLVGDHTGPSMWVSTFHSTCVRILRNQASLIGGL
HHCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAISNLKNELIDAESAVANLSSDT
CCCCEEEECCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DDLARTVATVYGEYQRRLRTANALDFDDLIGETVGILQAFPQIAEHYRRRFRHVLVDEYQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DTNHAQYVLVRELVGHGARNSPDDMPPGELCVVGDADQSIYAFRGSTISNIEDFERDYPD
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHCCCC
TTTILLEQNYRSTQNILSAANSVIARNFGRRDKRLWTDAGKGELIVGYVSDNEHDEAKFI
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
ADEIDALVGGGEITYDDVAVLYRANNLSRSLEEVFIPTGIPYKVVGGFCFYESKEIRDLI
HHHHHHHHCCCCEEHHHHEEEEECHHHHHHHHHHHCCCCCCHHHHCCCCEECCHHHHHHH
AYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACVSVYAENTGTSFADALQAVAEGKVPML
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
NTRSVKAIAGFVALLDDLRCRVDDDLGELVESVLERSGYLRELESSTDPQELARLDNLNE
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCHHH
LVSFAHEFSTEQANAAALAKSLHTPEDEDVPDTGALAAFLEKVSLMSDTDQIPENNSGVV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCCCCCCEE
TLMTLHAAKGLEFPVVFVTGWEDGMLPHMRTLGDPTELSEERRLAYVGITRARQRLYLSR
EEEEEHHCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCEEEEEHHHHHHHHHHHH
AITRSSWGQPILNPESRFLREIPPELIDWRRSILTDSYSTPASGASRFGRVRPSSIRSGA
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHCCC
SKRALLVLAPGDRVTHDKYGLGRVEEVSGVGESAISLIDFGSSGRIKLMHNHAPVTKL
CCCEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHEECCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002