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The map label for this gene is algU [H]

Identifier: 220919505

GI number: 220919505

Start: 4928161

End: 4928790

Strand: Direct

Name: algU [H]

Synonym: A2cp1_4426

Alternate gene names: 220919505

Gene position: 4928161-4928790 (Clockwise)

Preceding gene: 220919497

Following gene: 220919506

Centisome position: 97.99

GC content: 70.0

Gene sequence:

>630_bases
ATGGAAGCCGACGACCACGCGCTGGTCACGAGAGCCAAGGCCGGTGACGCCACCGCGTTCCGCGCGCTCGTCGTCCGCTA
CCAGAGGAAGGTCTACGCCGTGGCGCTGGGCATCGTGAAAGATCCGGACCTGGCCTGGGACGTCGCCCAGGAAGCCTTCG
TCCGCGTCCACGGCCACCTCGGCGACTTCAAGGGCGATTCGGCCTTCTCGACCTGGGTGTTCCGCATCACCACCCACCTC
TCGATCGACGCGGTGAGGCGGGAGCGGCACGCGCAGCGGCAGGACGTGGACGACGTGCAGGAGACGGATCTGGAGGAGGG
CGGCGAGGGCATCCTCTCGACCGCGCTCGGGAACGACCCGCGGCAGAACGCGCTCCGGCGCGAGCTGGCCGGCAAGATCC
AGGACGCGCTGGCGACGCTCCCGGAGAAGCACCGCACGATCCTGGTACTGCGCGAGGTCGAGGGTCTGTCGTACGAAGAG
CTGGCGGAGCGGCTCGGGATCCACAAGGGCACGGTCATGAGCCGGCTGTTCCACGCGCGGAAGAAGATGCAGGCCGCGCT
CCGCGAGTACGCGGGGATCGCGGCGGCGGACGGAGCCGAGGGCGGAGAGGACGGGCAGGGCGATGCGTAG

Upstream 100 bases:

>100_bases
TCGGCGCGGGCCGTCGGCGTCTAGACCCTCCCCCGGCGGCGCACGTGCTAGCCTGCCGCACCGTCCCGTGAATTTCCCAC
CCGGACGCCCCGTCCCTCCG

Downstream 100 bases:

>100_bases
GGCCACGAGCGATTGCGTCCGGTACGCGCCGATGATCGGCGCCCGCGAGGGCGAGCTCTCCACCGAGGAGGCGCGCGCGC
TCGCGGCGCACCTCGAGGCC

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MEADDHALVTRAKAGDATAFRALVVRYQRKVYAVALGIVKDPDLAWDVAQEAFVRVHGHLGDFKGDSAFSTWVFRITTHL
SIDAVRRERHAQRQDVDDVQETDLEEGGEGILSTALGNDPRQNALRRELAGKIQDALATLPEKHRTILVLREVEGLSYEE
LAERLGIHKGTVMSRLFHARKKMQAALREYAGIAAADGAEGGEDGQGDA

Sequences:

>Translated_209_residues
MEADDHALVTRAKAGDATAFRALVVRYQRKVYAVALGIVKDPDLAWDVAQEAFVRVHGHLGDFKGDSAFSTWVFRITTHL
SIDAVRRERHAQRQDVDDVQETDLEEGGEGILSTALGNDPRQNALRRELAGKIQDALATLPEKHRTILVLREVEGLSYEE
LAERLGIHKGTVMSRLFHARKKMQAALREYAGIAAADGAEGGEDGQGDA
>Mature_209_residues
MEADDHALVTRAKAGDATAFRALVVRYQRKVYAVALGIVKDPDLAWDVAQEAFVRVHGHLGDFKGDSAFSTWVFRITTHL
SIDAVRRERHAQRQDVDDVQETDLEEGGEGILSTALGNDPRQNALRRELAGKIQDALATLPEKHRTILVLREVEGLSYEE
LAERLGIHKGTVMSRLFHARKKMQAALREYAGIAAADGAEGGEDGQGDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=38.7434554973822, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22982; Mature: 22982

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADDHALVTRAKAGDATAFRALVVRYQRKVYAVALGIVKDPDLAWDVAQEAFVRVHGHL
CCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GDFKGDSAFSTWVFRITTHLSIDAVRRERHAQRQDVDDVQETDLEEGGEGILSTALGNDP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHCCCCHHHHHHCCCCH
RQNALRRELAGKIQDALATLPEKHRTILVLREVEGLSYEELAERLGIHKGTVMSRLFHAR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHH
KKMQAALREYAGIAAADGAEGGEDGQGDA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEADDHALVTRAKAGDATAFRALVVRYQRKVYAVALGIVKDPDLAWDVAQEAFVRVHGHL
CCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GDFKGDSAFSTWVFRITTHLSIDAVRRERHAQRQDVDDVQETDLEEGGEGILSTALGNDP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHCCCCHHHHHHCCCCH
RQNALRRELAGKIQDALATLPEKHRTILVLREVEGLSYEELAERLGIHKGTVMSRLFHAR
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHH
KKMQAALREYAGIAAADGAEGGEDGQGDA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]