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The map label for this gene is pyk [H]

Identifier: 220919469

GI number: 220919469

Start: 4884274

End: 4885743

Strand: Direct

Name: pyk [H]

Synonym: A2cp1_4390

Alternate gene names: 220919469

Gene position: 4884274-4885743 (Clockwise)

Preceding gene: 220919468

Following gene: 220919470

Centisome position: 97.12

GC content: 73.2

Gene sequence:

>1470_bases
ATGCGCCGCGCCAAGATCGTCGCCACCCTCGGACCCGCGTCCGGCGAGCCGGACGTGCTCGCCAGGCTCCTCGAGCAGGG
GGTGGACGTCGCCCGCCTGAACTTCTCCCACGGCCGGCACGAGGACCACGCGCGGATGCTCGACAAGATCCGCGCCGCCT
CGCGCCACCTCGGCAAGGCCGTGGCCGTGCTGCAGGACCTGCAGGGCCCGAAGATCCGCACCGGCCCGCTGAAGGCCGGG
AAGGCGGGCGTGCAGGTGGAGGCCGGGCAGGAGATCGTCATCACCACCGAGGGCGAGCTGGCGGGCGACGCGCACCTCGT
CTCCACCACCTACCCGCACCTCGCCGAGGACGTGCGCGCCGGCGACCGCCTGCTGGTGGACGACGGCCTGCTCGAGTTCC
GGGTGCTCGCCACCGACGGCGTGCGCGTGCGGGCCGAGGTGGTGGAGGGCGGCTGGCTCGGCGAGCACAAGGGGATCAAC
CTGCCCGGCGTGGCGCTCCGCACCGAGGCCATGTCCGAGAAGGACCGGGCCGACGTGGCGTTCGGCATCTCCCACGGCGT
GGACTACGTCGCGCTGTCGTTCGTGCGCACGCCCCAGGACATCGGCCTCTGCCGCGAGGAGATGGAGCGCGCCGGCCGGG
TGGTGCCCATCATCGCGAAGATCGAGAAGCCCGAGGCCATCGACAACCTCGACGCCATCATCGCCGCCGCCGACGGGGTG
ATGGTGGCCCGCGGCGATCTCGGCGTGGAGATCCTGCCGGAGCGCGTGCCGCTGCTGCAGAAGGAGATCTGCTCGAAGGC
GAACGCCTCCGGCAAGCCGGTCATCATCGCCACCCAGATGCTGAACTCGATGATGGAGCACCCGCGCCCCACCCGCGCCG
AGGCGTCCGACGTGGCGAACGGGATCTGGGACGGTGCCGACGCGGTGATGCTCTCCGGCGAGACCGCCAGCGGCCGCTTC
CCGCTCGCGGCGGTGCAGATGATGGACCGCATCGTGCGCGAGGCGGAGGCGGGCTCGCCGGCCGCGCTCCACGCGCGCGT
CCCGCCGCCGGCGCGCCCGGCGCCGTTCAACCTGGTGACCGCGTCGGCCGCCTGCGAGGCCGCCGAGGCCGCCGGGGCGG
TCGCGATCTGCTGCTTCACGCTGCGCGGCGAGACCGCGCGGCTGCTCGCGCACTTCCGCCCGCGGGTGCCGATCGTCGCG
TTCTCGCCGGACCAGTCCATCCGCCGGCGCCTGGCGCTGTACTGGGGCGTGCTGCCGAAGGTGATGGAGCCGGTGAAGAA
CGCCGACCTCATGGCCGAGATCGTCTCCGACCGGCTCCTCGAGGAGAAGCTGGTGAAGCCGGGCGACCGGGTGGTGCTGG
TGCACGGCTCGCCGCTCGGCCTGCCCGGCCAGACGAACTCCATCCGCCTGCACGAGATCACCCACCCGTCCGATCGCGGG
CCCACCCAGCGCTACCGCGTCCCGGTGTAG

Upstream 100 bases:

>100_bases
GAACGTGGTGCACGTCCAGTCGTGGCACCGGCGCGGCACCTGCGCCGGGCAAGCGGGATCGTGGACGCAGTTCGCGGCCT
GGCGTTAAAACACTCCTTCC

Downstream 100 bases:

>100_bases
CGCCGCCGGCCCCATCCGGCCCGCCGCCCCGGCGGCGTCCCCCTCGCAGGAGAACCGCATGGACATCCCCGCCCTGCAGC
GCGCGCTGGCCGAGTCGAAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRRAKIVATLGPASGEPDVLARLLEQGVDVARLNFSHGRHEDHARMLDKIRAASRHLGKAVAVLQDLQGPKIRTGPLKAG
KAGVQVEAGQEIVITTEGELAGDAHLVSTTYPHLAEDVRAGDRLLVDDGLLEFRVLATDGVRVRAEVVEGGWLGEHKGIN
LPGVALRTEAMSEKDRADVAFGISHGVDYVALSFVRTPQDIGLCREEMERAGRVVPIIAKIEKPEAIDNLDAIIAAADGV
MVARGDLGVEILPERVPLLQKEICSKANASGKPVIIATQMLNSMMEHPRPTRAEASDVANGIWDGADAVMLSGETASGRF
PLAAVQMMDRIVREAEAGSPAALHARVPPPARPAPFNLVTASAACEAAEAAGAVAICCFTLRGETARLLAHFRPRVPIVA
FSPDQSIRRRLALYWGVLPKVMEPVKNADLMAEIVSDRLLEEKLVKPGDRVVLVHGSPLGLPGQTNSIRLHEITHPSDRG
PTQRYRVPV

Sequences:

>Translated_489_residues
MRRAKIVATLGPASGEPDVLARLLEQGVDVARLNFSHGRHEDHARMLDKIRAASRHLGKAVAVLQDLQGPKIRTGPLKAG
KAGVQVEAGQEIVITTEGELAGDAHLVSTTYPHLAEDVRAGDRLLVDDGLLEFRVLATDGVRVRAEVVEGGWLGEHKGIN
LPGVALRTEAMSEKDRADVAFGISHGVDYVALSFVRTPQDIGLCREEMERAGRVVPIIAKIEKPEAIDNLDAIIAAADGV
MVARGDLGVEILPERVPLLQKEICSKANASGKPVIIATQMLNSMMEHPRPTRAEASDVANGIWDGADAVMLSGETASGRF
PLAAVQMMDRIVREAEAGSPAALHARVPPPARPAPFNLVTASAACEAAEAAGAVAICCFTLRGETARLLAHFRPRVPIVA
FSPDQSIRRRLALYWGVLPKVMEPVKNADLMAEIVSDRLLEEKLVKPGDRVVLVHGSPLGLPGQTNSIRLHEITHPSDRG
PTQRYRVPV
>Mature_489_residues
MRRAKIVATLGPASGEPDVLARLLEQGVDVARLNFSHGRHEDHARMLDKIRAASRHLGKAVAVLQDLQGPKIRTGPLKAG
KAGVQVEAGQEIVITTEGELAGDAHLVSTTYPHLAEDVRAGDRLLVDDGLLEFRVLATDGVRVRAEVVEGGWLGEHKGIN
LPGVALRTEAMSEKDRADVAFGISHGVDYVALSFVRTPQDIGLCREEMERAGRVVPIIAKIEKPEAIDNLDAIIAAADGV
MVARGDLGVEILPERVPLLQKEICSKANASGKPVIIATQMLNSMMEHPRPTRAEASDVANGIWDGADAVMLSGETASGRF
PLAAVQMMDRIVREAEAGSPAALHARVPPPARPAPFNLVTASAACEAAEAAGAVAICCFTLRGETARLLAHFRPRVPIVA
FSPDQSIRRRLALYWGVLPKVMEPVKNADLMAEIVSDRLLEEKLVKPGDRVVLVHGSPLGLPGQTNSIRLHEITHPSDRG
PTQRYRVPV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=40.4907975460123, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.6728016359918, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.6728016359918, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=40.3651115618661, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=40.3651115618661, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=40.418118466899, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=40.418118466899, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=37.4468085106383, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=37.4468085106383, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=37.3831775700935, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=40.0423728813559, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=39.5397489539749, Blast_Score=295, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=39.7916666666667, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=41.1042944785276, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=41.1042944785276, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=41.1042944785276, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=41.1042944785276, Blast_Score=300, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=37.037037037037, Blast_Score=303, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=36.0082304526749, Blast_Score=295, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=39.4366197183099, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=39.4366197183099, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=40.5867970660147, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24581235, Length=496, Percent_Identity=33.4677419354839, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=39.1791044776119, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52475; Mature: 52475

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAKIVATLGPASGEPDVLARLLEQGVDVARLNFSHGRHEDHARMLDKIRAASRHLGKA
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VAVLQDLQGPKIRTGPLKAGKAGVQVEAGQEIVITTEGELAGDAHLVSTTYPHLAEDVRA
HHHHHHCCCCCEECCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEECCCHHHHHHHCC
GDRLLVDDGLLEFRVLATDGVRVRAEVVEGGWLGEHKGINLPGVALRTEAMSEKDRADVA
CCEEEEECCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCEEEHHHHCCCCCCCEE
FGISHGVDYVALSFVRTPQDIGLCREEMERAGRVVPIIAKIEKPEAIDNLDAIIAAADGV
EEHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEECCCE
MVARGDLGVEILPERVPLLQKEICSKANASGKPVIIATQMLNSMMEHPRPTRAEASDVAN
EEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHH
GIWDGADAVMLSGETASGRFPLAAVQMMDRIVREAEAGSPAALHARVPPPARPAPFNLVT
HHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEE
ASAACEAAEAAGAVAICCFTLRGETARLLAHFRPRVPIVAFSPDQSIRRRLALYWGVLPK
HHHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHH
VMEPVKNADLMAEIVSDRLLEEKLVKPGDRVVLVHGSPLGLPGQTNSIRLHEITHPSDRG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
PTQRYRVPV
CCCCCCCCC
>Mature Secondary Structure
MRRAKIVATLGPASGEPDVLARLLEQGVDVARLNFSHGRHEDHARMLDKIRAASRHLGKA
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
VAVLQDLQGPKIRTGPLKAGKAGVQVEAGQEIVITTEGELAGDAHLVSTTYPHLAEDVRA
HHHHHHCCCCCEECCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEECCCHHHHHHHCC
GDRLLVDDGLLEFRVLATDGVRVRAEVVEGGWLGEHKGINLPGVALRTEAMSEKDRADVA
CCEEEEECCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCEEEHHHHCCCCCCCEE
FGISHGVDYVALSFVRTPQDIGLCREEMERAGRVVPIIAKIEKPEAIDNLDAIIAAADGV
EEHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEECCCE
MVARGDLGVEILPERVPLLQKEICSKANASGKPVIIATQMLNSMMEHPRPTRAEASDVAN
EEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHH
GIWDGADAVMLSGETASGRFPLAAVQMMDRIVREAEAGSPAALHARVPPPARPAPFNLVT
HHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEE
ASAACEAAEAAGAVAICCFTLRGETARLLAHFRPRVPIVAFSPDQSIRRRLALYWGVLPK
HHHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHH
VMEPVKNADLMAEIVSDRLLEEKLVKPGDRVVLVHGSPLGLPGQTNSIRLHEITHPSDRG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
PTQRYRVPV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]