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The map label for this gene is kinE [H]

Identifier: 220919452

GI number: 220919452

Start: 4866135

End: 4868198

Strand: Direct

Name: kinE [H]

Synonym: A2cp1_4373

Alternate gene names: 220919452

Gene position: 4866135-4868198 (Clockwise)

Preceding gene: 220919451

Following gene: 220919454

Centisome position: 96.76

GC content: 77.03

Gene sequence:

>2064_bases
ATGAGCGGGCGAGGCGAGGTTCGCGAGCGGATCCCGGGCGAGCGGTCCGGACCGGCGGACGGGGACGCGGTCCGCCGACG
CGCGCCGCTCGAGGCGGCGGTCGCGGCGCTCGCCGAGCTCGCCATGAACGCCGAGGGGACCGGGCCGCTGCTCACGGCCG
CCTGCGCCATCGCGCGGGACGCGCTCGGCGTCGATGCGGCCGCGTTCCTGCAGCCGGACGACGCCGCCGCCGGCGCGCTG
GTGGTCCGCGCCGCGGTGGGGCTCCCGCCCGACGCCGCGGGCGCGCGGGTGGGCGGCACGCCGCCGGACGAGAGCGCGGC
GGTGACCGGCGGTCACGGCGGCCGGCCGCCGCTGCTCGACCTCCCCGGCATCGAGGCCTCCGCAGAGGTGACGTTGCCCG
GGCGCGAGCGCCTGCTCGGCGTGATCGGTGCCTACGCCCGGCGCCCGCGGGCCTTCCACGCGGACGAGCTGCGCTTCCTC
GAGACGAGCGCCAGCGTGCTCGCGGCGGCGCTGGCGCGCGACCTCGCCGAGGCCGAGGTGCTGGACCGCGAGCGCCAGCT
GCGGGCGGTGTTCGACGCCGCGCTGGACGCGATGCTGTGCGTGGACGCGTCGGGCCGGGTCCGCGACGCGAACGCGGCCG
CGGCCGCGCTGTTCGGGGGCGGGCGCGCGAGCCTGGTCGGCCGCAGCCTGACGGACCTCACCGAGGCGCCGGCGCCCGGC
GCCCCGGCGCTCGCCGACGTCCTCCGCGGCGAGCGGGTGAGCGGCGCCGCCGAGGTGGTGCACGCGGGTGGGCTCCGCCG
CTCGGTCGAGTTCACCGGCGTGCCGGACATCCAGCCGGGCCGGCACCTGATCGCGCTCCGCGACGTGAGCGAGCGCAAGC
AGCTCCACGCCCGCCTCGCGCTCGCCGACCGCATGGTGTCGGTGGGCACGCTGGCCGCCGGGGTGGCGCACGAGCTCAAC
AACCCGCTCGCGTACGTGATCGCCAACCTCTCGTACCTGGACGAGCAGCTCACGGTCCTCGCCCCGCGCGTGGCCGCGGC
GGGGAGCTCGCGCGAGCCGGACCTCGCGAGCGCGCTCCTCGACGCCGTCCACGACGCGCGCGACGGCGCCGAGCGGATGC
GGATCATCGTCCGGGACCTGAAGACGTTTTCGCGCCCGGACGAGGAGCACGCCGGCCCCGTGTCGCTCGGGCCGCTGCTC
GACTCCTGCGTGAGCGTGGCCTGGAACGAGATCCGGCACCGGGCGCGCCTGCTGCGCGCGCCGACGGACGTGCCGCCGAT
CCAGGGGTGCCAGGCGCGGCTCGGGCAGGTGTTCCTCAACCTGCTCGTGAACGCCGCGCAGGCGATCCCGGAGGGCCACG
TGGACGAGCACCAGATCCAGGTCTCCGCCCGCGCGCTGCCCGGCGGCCAGGTGGCGGTGGAGGTGTCCGACACCGGCTCG
GGCATCGCGCCGGAGCACCTGCCGCGCATCTTCGACCCGTTCTTCACCACCAAGCCGCCGGGCGTGGGCACCGGGCTCGG
GCTGTCGATCTGCCAGAGCATCGTCTCGGCGATGGGCGGCGAGATCCAGGTGGAGACGATGCTGGGGCGGGGCACCACCT
TCCGCGTGATCCTGCCGGCGTCCGGGCCGGACGAGGCCGGCGCGTCGGGAGTGGGGACCGCGCACCTGGCGCCGCGGGTG
CGCGCGCGGATCCTGGTCGTGGACGACGAGCCGCTGGTGGGGACGGTGATCCAGCGGGCGCTCCAGGGCGAGCACGAGGT
GACGGTCACGTCGAGCGCGCGCGCCGCGCTGGCGCGGGTGGCGGCGGGGGAGCGGTTCGACCTGGTCCTCTCCGACCTGC
TCATGCCGGAGATGACCGGGATGGAGCTGTACCGCGCGCTGCGCGAGCGCGCACCGGAGCTGGCGAGCCGCGTGGTGTTC
CTCACCGGCGGCGCCTTCACCCCCGCCGCCCGCACCTTCCTGGAGCAGGAGCCGGTGGAGTGCGTGGAGAAGCCGTTCGA
GCTGGAGACGATCCGGGCGCTGCTGGCGCGCCGGCTCGGCGCCCGCCGGGCCGACGCCTGCTGA

Upstream 100 bases:

>100_bases
CGGGTCCGTGTCGCCCCGGGTGCCGCTCGGATGCCCGCGGCGCGGGGCGCGCGCCGACGAGCCGGATCGCGCGACGCCAC
GGGCCCGAGAGGTTGCGGCG

Downstream 100 bases:

>100_bases
CGCTTCTCCGCGCGCCGCAGGCGCGCCCGCGGGGACCTCGCGCGTGCCCGCGCAGGCGGGCACGCGAGGTCACGGGCCCC
GCGAAGCAGGGGTGGGCCCC

Product: multi-sensor hybrid histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSGRGEVRERIPGERSGPADGDAVRRRAPLEAAVAALAELAMNAEGTGPLLTAACAIARDALGVDAAAFLQPDDAAAGAL
VVRAAVGLPPDAAGARVGGTPPDESAAVTGGHGGRPPLLDLPGIEASAEVTLPGRERLLGVIGAYARRPRAFHADELRFL
ETSASVLAAALARDLAEAEVLDRERQLRAVFDAALDAMLCVDASGRVRDANAAAAALFGGGRASLVGRSLTDLTEAPAPG
APALADVLRGERVSGAAEVVHAGGLRRSVEFTGVPDIQPGRHLIALRDVSERKQLHARLALADRMVSVGTLAAGVAHELN
NPLAYVIANLSYLDEQLTVLAPRVAAAGSSREPDLASALLDAVHDARDGAERMRIIVRDLKTFSRPDEEHAGPVSLGPLL
DSCVSVAWNEIRHRARLLRAPTDVPPIQGCQARLGQVFLNLLVNAAQAIPEGHVDEHQIQVSARALPGGQVAVEVSDTGS
GIAPEHLPRIFDPFFTTKPPGVGTGLGLSICQSIVSAMGGEIQVETMLGRGTTFRVILPASGPDEAGASGVGTAHLAPRV
RARILVVDDEPLVGTVIQRALQGEHEVTVTSSARAALARVAAGERFDLVLSDLLMPEMTGMELYRALRERAPELASRVVF
LTGGAFTPAARTFLEQEPVECVEKPFELETIRALLARRLGARRADAC

Sequences:

>Translated_687_residues
MSGRGEVRERIPGERSGPADGDAVRRRAPLEAAVAALAELAMNAEGTGPLLTAACAIARDALGVDAAAFLQPDDAAAGAL
VVRAAVGLPPDAAGARVGGTPPDESAAVTGGHGGRPPLLDLPGIEASAEVTLPGRERLLGVIGAYARRPRAFHADELRFL
ETSASVLAAALARDLAEAEVLDRERQLRAVFDAALDAMLCVDASGRVRDANAAAAALFGGGRASLVGRSLTDLTEAPAPG
APALADVLRGERVSGAAEVVHAGGLRRSVEFTGVPDIQPGRHLIALRDVSERKQLHARLALADRMVSVGTLAAGVAHELN
NPLAYVIANLSYLDEQLTVLAPRVAAAGSSREPDLASALLDAVHDARDGAERMRIIVRDLKTFSRPDEEHAGPVSLGPLL
DSCVSVAWNEIRHRARLLRAPTDVPPIQGCQARLGQVFLNLLVNAAQAIPEGHVDEHQIQVSARALPGGQVAVEVSDTGS
GIAPEHLPRIFDPFFTTKPPGVGTGLGLSICQSIVSAMGGEIQVETMLGRGTTFRVILPASGPDEAGASGVGTAHLAPRV
RARILVVDDEPLVGTVIQRALQGEHEVTVTSSARAALARVAAGERFDLVLSDLLMPEMTGMELYRALRERAPELASRVVF
LTGGAFTPAARTFLEQEPVECVEKPFELETIRALLARRLGARRADAC
>Mature_686_residues
SGRGEVRERIPGERSGPADGDAVRRRAPLEAAVAALAELAMNAEGTGPLLTAACAIARDALGVDAAAFLQPDDAAAGALV
VRAAVGLPPDAAGARVGGTPPDESAAVTGGHGGRPPLLDLPGIEASAEVTLPGRERLLGVIGAYARRPRAFHADELRFLE
TSASVLAAALARDLAEAEVLDRERQLRAVFDAALDAMLCVDASGRVRDANAAAAALFGGGRASLVGRSLTDLTEAPAPGA
PALADVLRGERVSGAAEVVHAGGLRRSVEFTGVPDIQPGRHLIALRDVSERKQLHARLALADRMVSVGTLAAGVAHELNN
PLAYVIANLSYLDEQLTVLAPRVAAAGSSREPDLASALLDAVHDARDGAERMRIIVRDLKTFSRPDEEHAGPVSLGPLLD
SCVSVAWNEIRHRARLLRAPTDVPPIQGCQARLGQVFLNLLVNAAQAIPEGHVDEHQIQVSARALPGGQVAVEVSDTGSG
IAPEHLPRIFDPFFTTKPPGVGTGLGLSICQSIVSAMGGEIQVETMLGRGTTFRVILPASGPDEAGASGVGTAHLAPRVR
ARILVVDDEPLVGTVIQRALQGEHEVTVTSSARAALARVAAGERFDLVLSDLLMPEMTGMELYRALRERAPELASRVVFL
TGGAFTPAARTFLEQEPVECVEKPFELETIRALLARRLGARRADAC

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=360, Percent_Identity=29.7222222222222, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790436, Length=257, Percent_Identity=35.0194552529183, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994928, Length=465, Percent_Identity=27.3118279569892, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081816, Length=282, Percent_Identity=30.4964539007092, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=324, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786600, Length=356, Percent_Identity=25.561797752809, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=250, Percent_Identity=28, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=30.1255230125523, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=252, Percent_Identity=28.1746031746032, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71761; Mature: 71629

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRGEVRERIPGERSGPADGDAVRRRAPLEAAVAALAELAMNAEGTGPLLTAACAIARD
CCCCCCHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALGVDAAAFLQPDDAAAGALVVRAAVGLPPDAAGARVGGTPPDESAAVTGGHGGRPPLLD
HCCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEE
LPGIEASAEVTLPGRERLLGVIGAYARRPRAFHADELRFLETSASVLAAALARDLAEAEV
CCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRERQLRAVFDAALDAMLCVDASGRVRDANAAAAALFGGGRASLVGRSLTDLTEAPAPG
HHHHHHHHHHHHHHHHHHHEECCCCCEECCCHHHHHHHCCCHHHHHCCHHHHHHCCCCCC
APALADVLRGERVSGAAEVVHAGGLRRSVEFTGVPDIQPGRHLIALRDVSERKQLHARLA
CHHHHHHHCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCHHHHHHHHHHHH
LADRMVSVGTLAAGVAHELNNPLAYVIANLSYLDEQLTVLAPRVAAAGSSREPDLASALL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DAVHDARDGAERMRIIVRDLKTFSRPDEEHAGPVSLGPLLDSCVSVAWNEIRHRARLLRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PTDVPPIQGCQARLGQVFLNLLVNAAQAIPEGHVDEHQIQVSARALPGGQVAVEVSDTGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCC
GIAPEHLPRIFDPFFTTKPPGVGTGLGLSICQSIVSAMGGEIQVETMLGRGTTFRVILPA
CCCHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEC
SGPDEAGASGVGTAHLAPRVRARILVVDDEPLVGTVIQRALQGEHEVTVTSSARAALARV
CCCCCCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AAGERFDLVLSDLLMPEMTGMELYRALRERAPELASRVVFLTGGAFTPAARTFLEQEPVE
HCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCHH
CVEKPFELETIRALLARRLGARRADAC
HHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGRGEVRERIPGERSGPADGDAVRRRAPLEAAVAALAELAMNAEGTGPLLTAACAIARD
CCCCCHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALGVDAAAFLQPDDAAAGALVVRAAVGLPPDAAGARVGGTPPDESAAVTGGHGGRPPLLD
HCCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEE
LPGIEASAEVTLPGRERLLGVIGAYARRPRAFHADELRFLETSASVLAAALARDLAEAEV
CCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRERQLRAVFDAALDAMLCVDASGRVRDANAAAAALFGGGRASLVGRSLTDLTEAPAPG
HHHHHHHHHHHHHHHHHHHEECCCCCEECCCHHHHHHHCCCHHHHHCCHHHHHHCCCCCC
APALADVLRGERVSGAAEVVHAGGLRRSVEFTGVPDIQPGRHLIALRDVSERKQLHARLA
CHHHHHHHCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCHHHHHHHHHHHH
LADRMVSVGTLAAGVAHELNNPLAYVIANLSYLDEQLTVLAPRVAAAGSSREPDLASALL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
DAVHDARDGAERMRIIVRDLKTFSRPDEEHAGPVSLGPLLDSCVSVAWNEIRHRARLLRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PTDVPPIQGCQARLGQVFLNLLVNAAQAIPEGHVDEHQIQVSARALPGGQVAVEVSDTGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEEECCCC
GIAPEHLPRIFDPFFTTKPPGVGTGLGLSICQSIVSAMGGEIQVETMLGRGTTFRVILPA
CCCHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEC
SGPDEAGASGVGTAHLAPRVRARILVVDDEPLVGTVIQRALQGEHEVTVTSSARAALARV
CCCCCCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AAGERFDLVLSDLLMPEMTGMELYRALRERAPELASRVVFLTGGAFTPAARTFLEQEPVE
HCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHCCCHH
CVEKPFELETIRALLARRLGARRADAC
HHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]