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The map label for this gene is mraW

Identifier: 220918988

GI number: 220918988

Start: 4340950

End: 4341882

Strand: Direct

Name: mraW

Synonym: A2cp1_3903

Alternate gene names: 220918988

Gene position: 4340950-4341882 (Clockwise)

Preceding gene: 220918987

Following gene: 220918989

Centisome position: 86.31

GC content: 75.78

Gene sequence:

>933_bases
GTGAGCGCGGACTTTCGCCATGAGCCGGTACTCGCGAACGAGATCCTGGAGCTCCTGCGCCCCCGGCCGGGCGAGCTCTT
CCTCGACGGCACGCTCGGCGGCGGCGGCCACTCCGGCCTGCTGCTCGAGGCGGGGGCGCGCGTGATCGCCCTCGACAAGG
ATCCCCGCGCGCTCGCCGCGGCCACCGCCCGGCTCGCCCGCTTCGGCGAGGCGTTCCGCGCGGTGCGCTCCGACTTCCGC
GACGCGAAGAACGTGCTCGAGGCGCTCGGCATCGCCGCGGTGGACGGCGCGCTGGTGGACCTGGGCGTCTCCTCGCCCCA
GCTCGACGAGGCGGAGCGCGGCTTCTCCTTCTCGCGCCCCGGGCCGCTCGACATGCGCATGGGCGACACCGGCGAGACGC
TCGAGGACCTGCTGCGCCGGATCGACGAGCGCGAGCTGGCCCGCATCCTGCGCGAGTACGGCGAGGAGCCGTTCGCGCGG
CCGGTGGCGCGCGCCGTCAAGGCGGCGCTGGAGACCGAGGCGCCGCTCGACACCGCGCGCCTCGCCGAGGTGGTCGCCGG
CGCGATCCCGCGCAAGGCCTGGCCGCACCGGATCCACCCCGCCACCCGCACGTTCCAGGCGCTGCGGATCGCGGTGAACG
ACGAGCTCGGCGCGCTGGCCGCGTGGCTGGACGGCCTGCCCGGCGTCCTCGCCCCCGGCGGGCGCGCGGCGGCCATCTCG
TTCCACTCGCTCGAGGACCGGATGGTGAAGGAGAAGTTCCGGGCGCTGACGCAGGCCTGCACCTGCCCGCCGGACCTGCC
GGTGTGCGCCTGCGGGGCGAAGGCCTCGTTCGCGGCCATCACGCGCAAGGCCGTCAAGGCGTCCGACGAGGAGATCGCGC
GCAACCCGCGCGCCCGGAGCGCGCGCCTGCGCGCGGTGGAGAAGCTGCGATGA

Upstream 100 bases:

>100_bases
CCGCGGGCGCGAGCCGCTACGTGCGCGACCTGGTCCACGCCGGCGGCGCGGGCGGCTACGTGGAGCTGTACGGCGACGTG
GAGGAGGCCGTCCGGGCGGC

Downstream 100 bases:

>100_bases
GCGCCCGCGCCCCCGCGCGCCGCGCCGCGCCGGGCCTCCCCGGCGCCCGGCTGGCCGGCGAGATCGCGCTCGCGGCCGCG
CTGCTCGCGGCGCTGGGCGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSADFRHEPVLANEILELLRPRPGELFLDGTLGGGGHSGLLLEAGARVIALDKDPRALAAATARLARFGEAFRAVRSDFR
DAKNVLEALGIAAVDGALVDLGVSSPQLDEAERGFSFSRPGPLDMRMGDTGETLEDLLRRIDERELARILREYGEEPFAR
PVARAVKAALETEAPLDTARLAEVVAGAIPRKAWPHRIHPATRTFQALRIAVNDELGALAAWLDGLPGVLAPGGRAAAIS
FHSLEDRMVKEKFRALTQACTCPPDLPVCACGAKASFAAITRKAVKASDEEIARNPRARSARLRAVEKLR

Sequences:

>Translated_310_residues
MSADFRHEPVLANEILELLRPRPGELFLDGTLGGGGHSGLLLEAGARVIALDKDPRALAAATARLARFGEAFRAVRSDFR
DAKNVLEALGIAAVDGALVDLGVSSPQLDEAERGFSFSRPGPLDMRMGDTGETLEDLLRRIDERELARILREYGEEPFAR
PVARAVKAALETEAPLDTARLAEVVAGAIPRKAWPHRIHPATRTFQALRIAVNDELGALAAWLDGLPGVLAPGGRAAAIS
FHSLEDRMVKEKFRALTQACTCPPDLPVCACGAKASFAAITRKAVKASDEEIARNPRARSARLRAVEKLR
>Mature_309_residues
SADFRHEPVLANEILELLRPRPGELFLDGTLGGGGHSGLLLEAGARVIALDKDPRALAAATARLARFGEAFRAVRSDFRD
AKNVLEALGIAAVDGALVDLGVSSPQLDEAERGFSFSRPGPLDMRMGDTGETLEDLLRRIDERELARILREYGEEPFARP
VARAVKAALETEAPLDTARLAEVVAGAIPRKAWPHRIHPATRTFQALRIAVNDELGALAAWLDGLPGVLAPGGRAAAISF
HSLEDRMVKEKFRALTQACTCPPDLPVCACGAKASFAAITRKAVKASDEEIARNPRARSARLRAVEKLR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=39.4658753709199, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=40.2116402116402, Blast_Score=128, Evalue=5e-30,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=43.3962264150943, Blast_Score=212, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=38.1818181818182, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=38.1818181818182, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_ANAD2 (B8J8E0)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002494292.1
- ProteinModelPortal:   B8J8E0
- SMR:   B8J8E0
- GeneID:   7297243
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3903
- HOGENOM:   HBG302779
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33387; Mature: 33255

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADFRHEPVLANEILELLRPRPGELFLDGTLGGGGHSGLLLEAGARVIALDKDPRALAA
CCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHH
ATARLARFGEAFRAVRSDFRDAKNVLEALGIAAVDGALVDLGVSSPQLDEAERGFSFSRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHCCCCCCCC
GPLDMRMGDTGETLEDLLRRIDERELARILREYGEEPFARPVARAVKAALETEAPLDTAR
CCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
LAEVVAGAIPRKAWPHRIHPATRTFQALRIAVNDELGALAAWLDGLPGVLAPGGRAAAIS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE
FHSLEDRMVKEKFRALTQACTCPPDLPVCACGAKASFAAITRKAVKASDEEIARNPRARS
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHH
ARLRAVEKLR
HHHHHHHHCC
>Mature Secondary Structure 
SADFRHEPVLANEILELLRPRPGELFLDGTLGGGGHSGLLLEAGARVIALDKDPRALAA
CCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCEEEEECCCCHHHHH
ATARLARFGEAFRAVRSDFRDAKNVLEALGIAAVDGALVDLGVSSPQLDEAERGFSFSRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHCCCCCCCC
GPLDMRMGDTGETLEDLLRRIDERELARILREYGEEPFARPVARAVKAALETEAPLDTAR
CCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
LAEVVAGAIPRKAWPHRIHPATRTFQALRIAVNDELGALAAWLDGLPGVLAPGGRAAAIS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEE
FHSLEDRMVKEKFRALTQACTCPPDLPVCACGAKASFAAITRKAVKASDEEIARNPRARS
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHH
ARLRAVEKLR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA