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The map label for this gene is trpB2 [H]

Identifier: 220918974

GI number: 220918974

Start: 4325646

End: 4327001

Strand: Direct

Name: trpB2 [H]

Synonym: A2cp1_3889

Alternate gene names: 220918974

Gene position: 4325646-4327001 (Clockwise)

Preceding gene: 220918972

Following gene: 220918975

Centisome position: 86.01

GC content: 70.65

Gene sequence:

>1356_bases
ATGCAGACGAAGTTCCTGCTCGGCGAGAACCAGATCCCCACCCACTGGTACAACGTCATCCCGGACCTCCCGGAGCCGCT
GTCCCCGGTGATCCACCCCGCCACCGGCAAGCCGGCCAGCCCGGACGACCTGGTCCGGATCTTCCCGCCGCAGCTCATCG
AGCAGGAGGTCTCGACCCAGCGCTGGATCCAGATCCCCGAGCCGGTCCGCGAGATCTACCGGCTGTGGCGGCCGGCCCCG
CTGTTCCGCGCCCACCGCCTGGAGAAGGCGCTCGGGACCCCCGCGCACATCTACTACAAGTACGAGGGCGTCTCGCCGGC
CGGCTCGCACAAGCCGAACACCGCGGTGCCGCAGGCCTACTACAACAAGCTCGCCGGCACGAAGCGCCTCGCCACCGAGA
CCGGCGCCGGACAGTGGGGCTCGTCGATCGCGCTCGCGGCGCAGATGTTCGGGCTCGCCTGCACCGTCTACATGGTGAAG
GTGTCGTACGGGCAGAAGCCGTACCGCCGCAGCATGATGCAGCTCTGGGGCGCGGAGGTGATCCCCTCGCCCTCCGACCG
CACCGCCGCGGGCCGCGGGATCCTGGCCGCGGATCCGGAGAGCCCCGGCTCGCTCGGCGTGGCGATCTCCGAGGCGGTGG
AGGACGCGGTCACCCACGAGGACACGCGCTACGCGCTGGGCAGCGTGCTGAACCACGTCTGCCTGCACCAGACCGTGATC
GGGCTCGAGGCGAAGGAGCAGCTCAAGCTCGCCGGCGAGTACCCGGACGTCGTCATCGGGTGCCACGGCGGCGGCTCCAA
CTTCGCCGGCATCGGCTTCCCGTTCCTCGCCGACAAGGCCGCCGGCAAGGACGTCCGCATCCTCGCCATCGAGCCGTCGT
CCTGCCCCACGCTGACGAAGGGCGCGTACACGTTCGACTACGGCGACACCGCCAAGATGGCGCCCATCATGCGGATGTAC
ACGCTGGGTCACGACTTCATGCCGCCCGGCATCCACGCCGGCGGCCTGCGCTACCACGGCGCGTCGCCGCTCGTGTCGCA
GCTCGTGCACGCGGGCCTGGTGGAGGCGCGCGCGGTGGGGCAGCTCGCCTGCTTCGAGGCGGCGGTGCAGTTCGCCCGGG
CCGAGGGGATCATCCCCGCGCCGGAGTCGTCGCACGCGATCCGCGGCGCGGTCGAGGAGGCGCTGCGCGCGAAGGAGGAG
GGGAGGGAGCGCGTCATCCTCTTCAACCTCTCCGGCCACGGCCACGTGGACATGACGTCGTACGACCAGTTCCTCTCCGG
CAAGCTCGACGACTTCGAGTACCCGGAGGAGAAGGTGCGCGCGTCGCTCGCGCACCTGCCCAAGGTCGAGCTGTAG

Upstream 100 bases:

>100_bases
CGGTCGCCTTGACGCCCCTGTCGGGCTCTGCGCTAATGATCGATTTCTCGCGCCGCGCCCTCGGGCCTGCGGCGCCGGCC
ATCCCTCCTGCGAGGCGCGA

Downstream 100 bases:

>100_bases
GCGAGGCCGCCGGGCCGCGCGGGCGCCCGCGCGTGCCCGGTGACGCGTCGCGCGAGCGAGCGCGCGCCGGGAGCACCTTC
GGTGCGACGACCCAACCGAG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQTKFLLGENQIPTHWYNVIPDLPEPLSPVIHPATGKPASPDDLVRIFPPQLIEQEVSTQRWIQIPEPVREIYRLWRPAP
LFRAHRLEKALGTPAHIYYKYEGVSPAGSHKPNTAVPQAYYNKLAGTKRLATETGAGQWGSSIALAAQMFGLACTVYMVK
VSYGQKPYRRSMMQLWGAEVIPSPSDRTAAGRGILAADPESPGSLGVAISEAVEDAVTHEDTRYALGSVLNHVCLHQTVI
GLEAKEQLKLAGEYPDVVIGCHGGGSNFAGIGFPFLADKAAGKDVRILAIEPSSCPTLTKGAYTFDYGDTAKMAPIMRMY
TLGHDFMPPGIHAGGLRYHGASPLVSQLVHAGLVEARAVGQLACFEAAVQFARAEGIIPAPESSHAIRGAVEEALRAKEE
GRERVILFNLSGHGHVDMTSYDQFLSGKLDDFEYPEEKVRASLAHLPKVEL

Sequences:

>Translated_451_residues
MQTKFLLGENQIPTHWYNVIPDLPEPLSPVIHPATGKPASPDDLVRIFPPQLIEQEVSTQRWIQIPEPVREIYRLWRPAP
LFRAHRLEKALGTPAHIYYKYEGVSPAGSHKPNTAVPQAYYNKLAGTKRLATETGAGQWGSSIALAAQMFGLACTVYMVK
VSYGQKPYRRSMMQLWGAEVIPSPSDRTAAGRGILAADPESPGSLGVAISEAVEDAVTHEDTRYALGSVLNHVCLHQTVI
GLEAKEQLKLAGEYPDVVIGCHGGGSNFAGIGFPFLADKAAGKDVRILAIEPSSCPTLTKGAYTFDYGDTAKMAPIMRMY
TLGHDFMPPGIHAGGLRYHGASPLVSQLVHAGLVEARAVGQLACFEAAVQFARAEGIIPAPESSHAIRGAVEEALRAKEE
GRERVILFNLSGHGHVDMTSYDQFLSGKLDDFEYPEEKVRASLAHLPKVEL
>Mature_451_residues
MQTKFLLGENQIPTHWYNVIPDLPEPLSPVIHPATGKPASPDDLVRIFPPQLIEQEVSTQRWIQIPEPVREIYRLWRPAP
LFRAHRLEKALGTPAHIYYKYEGVSPAGSHKPNTAVPQAYYNKLAGTKRLATETGAGQWGSSIALAAQMFGLACTVYMVK
VSYGQKPYRRSMMQLWGAEVIPSPSDRTAAGRGILAADPESPGSLGVAISEAVEDAVTHEDTRYALGSVLNHVCLHQTVI
GLEAKEQLKLAGEYPDVVIGCHGGGSNFAGIGFPFLADKAAGKDVRILAIEPSSCPTLTKGAYTFDYGDTAKMAPIMRMY
TLGHDFMPPGIHAGGLRYHGASPLVSQLVHAGLVEARAVGQLACFEAAVQFARAEGIIPAPESSHAIRGAVEEALRAKEE
GRERVILFNLSGHGHVDMTSYDQFLSGKLDDFEYPEEKVRASLAHLPKVEL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=398, Percent_Identity=29.3969849246231, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=373, Percent_Identity=31.9034852546917, Blast_Score=126, Evalue=8e-30,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49007; Mature: 49007

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKFLLGENQIPTHWYNVIPDLPEPLSPVIHPATGKPASPDDLVRIFPPQLIEQEVSTQ
CCCEEEECCCCCCHHHHHHCCCCCCCHHHCEECCCCCCCCCCCCEEECCHHHHHHHHHHH
RWIQIPEPVREIYRLWRPAPLFRAHRLEKALGTPAHIYYKYEGVSPAGSHKPNTAVPQAY
HEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKLAGTKRLATETGAGQWGSSIALAAQMFGLACTVYMVKVSYGQKPYRRSMMQLWGAEV
HHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCC
IPSPSDRTAAGRGILAADPESPGSLGVAISEAVEDAVTHEDTRYALGSVLNHVCLHQTVI
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLEAKEQLKLAGEYPDVVIGCHGGGSNFAGIGFPFLADKAAGKDVRILAIEPSSCPTLTK
CCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCC
GAYTFDYGDTAKMAPIMRMYTLGHDFMPPGIHAGGLRYHGASPLVSQLVHAGLVEARAVG
CCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
QLACFEAAVQFARAEGIIPAPESSHAIRGAVEEALRAKEEGRERVILFNLSGHGHVDMTS
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHH
YDQFLSGKLDDFEYPEEKVRASLAHLPKVEL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTKFLLGENQIPTHWYNVIPDLPEPLSPVIHPATGKPASPDDLVRIFPPQLIEQEVSTQ
CCCEEEECCCCCCHHHHHHCCCCCCCHHHCEECCCCCCCCCCCCEEECCHHHHHHHHHHH
RWIQIPEPVREIYRLWRPAPLFRAHRLEKALGTPAHIYYKYEGVSPAGSHKPNTAVPQAY
HEEECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHH
YNKLAGTKRLATETGAGQWGSSIALAAQMFGLACTVYMVKVSYGQKPYRRSMMQLWGAEV
HHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCC
IPSPSDRTAAGRGILAADPESPGSLGVAISEAVEDAVTHEDTRYALGSVLNHVCLHQTVI
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GLEAKEQLKLAGEYPDVVIGCHGGGSNFAGIGFPFLADKAAGKDVRILAIEPSSCPTLTK
CCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCC
GAYTFDYGDTAKMAPIMRMYTLGHDFMPPGIHAGGLRYHGASPLVSQLVHAGLVEARAVG
CCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
QLACFEAAVQFARAEGIIPAPESSHAIRGAVEEALRAKEEGRERVILFNLSGHGHVDMTS
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHH
YDQFLSGKLDDFEYPEEKVRASLAHLPKVEL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]