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The map label for this gene is ilvK [H]

Identifier: 220918669

GI number: 220918669

Start: 3996716

End: 3997777

Strand: Reverse

Name: ilvK [H]

Synonym: A2cp1_3577

Alternate gene names: 220918669

Gene position: 3997777-3996716 (Counterclockwise)

Preceding gene: 220918671

Following gene: 220918667

Centisome position: 79.49

GC content: 75.52

Gene sequence:

>1062_bases
ATGGGCATCCCCGTGGAGCGCACCCGCAGCCCGAAGCCGCACCCCGCCGACGCCGACCTCGGCTTCGGCCGCCACTTCAC
CGACCACCTGTTCCGGATCGACTGGTCCGAGGGGAAGGGCTGGCACGCGCCGCGGGTCGAGCCGTACCGGCCGCTCTCGA
TCGATCCGGCCGCCTCGGTGCTGCACTACGGGCAGGCCATCTTCGAGGGGCTGAAGGCGTTCCGGCACCGCGACGGCGGC
ATGCGCCTGTTCCGGCCGGAGGCGAACCTGGCGCGGCTCGCGGCGAGCGCGCGCCGGCTCAGCATGCCGGCGCCGGAGCC
GGGCATCGTGCTGGAGGGGATCCGCGCGCTGCTCCGCACCGACGCGGGCTGGCTGCCGGAGTCGCCCGGCACCGCGCTGT
ACGTGCGGCCGGTGATGTTCGCGACCGAGGCGTTCCTGGGCGTGCGGCCCTCCAAGACGTACACGCTGCTCGTGATCGTC
TCGCCGGTGGGCGCCTACTTCTCGGGCGGCGCCCACGCGCTGCGCATCTGGGTCGAGCGGGCGCGCGCCCGCGCCGCGCA
GGGCGGCATCGGCGCGTCCAAGGCGGCCGCGAACTACGTGGCCAGCCTGCTCGCGGGCGAGGAGGCGAAGGCGCACGGGT
ACGACCAGGTGCTCTGGCTCGACGCGGCCCGGCACGCCGATCTCGAGGAGATCGGGACCATGAACGTGTTCGCGAAGCTC
GGGGCGACGGTGGTCACCCCCGCGCTCGAGGGCACCATCCTCGCCGGCATCACCCGCGACTGCGTCATCACGCTGCTGCG
CGACTGGAACGTGCCGGTCGAGGAGCGCCGGGTCTCGCTCGCGGAGCTGGAGCGCGCGCACGCCGCCGGGGCGCTCGAGG
AGCTGTTCGGCACCGGCACCGCGGCGGTGGTGGCGCCCATCGGCGAGCTCGCCTGGGACGGGCGGCAGCTCGTCCTCCCC
GCGCCCGGCCCGCAGTCGGTCGGCGAGCGGCTGCGCGCGGCCGTGCACGCCGTCCAGCGCGGCGAGGCGCCGGATCCGCA
CGGCTGGCTCGAGCCGGTGTAG

Upstream 100 bases:

>100_bases
TCATGTTGACGCTCACGGTCCGGGCGGCTGTAATACGTCAACGGCATTGACCTGAGCCGCGCCGGCGCACCAGTGTAGCA
CCGGGCGAGCGGAGGACACG

Downstream 100 bases:

>100_bases
CGGGGAGGCGCGGCTAGATCGCGACCGGCGCCCGATCCACCAGCGTGCTCGCCTCCACCGCGCGCCCGCCGCGCAGGTAG
AGGCGCGGCACGCGGGCGGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MGIPVERTRSPKPHPADADLGFGRHFTDHLFRIDWSEGKGWHAPRVEPYRPLSIDPAASVLHYGQAIFEGLKAFRHRDGG
MRLFRPEANLARLAASARRLSMPAPEPGIVLEGIRALLRTDAGWLPESPGTALYVRPVMFATEAFLGVRPSKTYTLLVIV
SPVGAYFSGGAHALRIWVERARARAAQGGIGASKAAANYVASLLAGEEAKAHGYDQVLWLDAARHADLEEIGTMNVFAKL
GATVVTPALEGTILAGITRDCVITLLRDWNVPVEERRVSLAELERAHAAGALEELFGTGTAAVVAPIGELAWDGRQLVLP
APGPQSVGERLRAAVHAVQRGEAPDPHGWLEPV

Sequences:

>Translated_353_residues
MGIPVERTRSPKPHPADADLGFGRHFTDHLFRIDWSEGKGWHAPRVEPYRPLSIDPAASVLHYGQAIFEGLKAFRHRDGG
MRLFRPEANLARLAASARRLSMPAPEPGIVLEGIRALLRTDAGWLPESPGTALYVRPVMFATEAFLGVRPSKTYTLLVIV
SPVGAYFSGGAHALRIWVERARARAAQGGIGASKAAANYVASLLAGEEAKAHGYDQVLWLDAARHADLEEIGTMNVFAKL
GATVVTPALEGTILAGITRDCVITLLRDWNVPVEERRVSLAELERAHAAGALEELFGTGTAAVVAPIGELAWDGRQLVLP
APGPQSVGERLRAAVHAVQRGEAPDPHGWLEPV
>Mature_352_residues
GIPVERTRSPKPHPADADLGFGRHFTDHLFRIDWSEGKGWHAPRVEPYRPLSIDPAASVLHYGQAIFEGLKAFRHRDGGM
RLFRPEANLARLAASARRLSMPAPEPGIVLEGIRALLRTDAGWLPESPGTALYVRPVMFATEAFLGVRPSKTYTLLVIVS
PVGAYFSGGAHALRIWVERARARAAQGGIGASKAAANYVASLLAGEEAKAHGYDQVLWLDAARHADLEEIGTMNVFAKLG
ATVVTPALEGTILAGITRDCVITLLRDWNVPVEERRVSLAELERAHAAGALEELFGTGTAAVVAPIGELAWDGRQLVLPA
PGPQSVGERLRAAVHAVQRGEAPDPHGWLEPV

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=361, Percent_Identity=38.2271468144044, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI296010904, Length=339, Percent_Identity=37.7581120943953, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI296010906, Length=339, Percent_Identity=37.7581120943953, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI38176287, Length=339, Percent_Identity=37.7581120943953, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI258614015, Length=296, Percent_Identity=38.1756756756757, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI296010900, Length=339, Percent_Identity=32.4483775811209, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI296010902, Length=339, Percent_Identity=32.4483775811209, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI48994963, Length=300, Percent_Identity=32.3333333333333, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=357, Percent_Identity=35.2941176470588, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17565728, Length=324, Percent_Identity=37.6543209876543, Blast_Score=210, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6322608, Length=345, Percent_Identity=38.2608695652174, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322002, Length=333, Percent_Identity=40.5405405405405, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24641779, Length=346, Percent_Identity=37.2832369942197, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 38021; Mature: 37890

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIPVERTRSPKPHPADADLGFGRHFTDHLFRIDWSEGKGWHAPRVEPYRPLSIDPAASV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
LHYGQAIFEGLKAFRHRDGGMRLFRPEANLARLAASARRLSMPAPEPGIVLEGIRALLRT
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
DAGWLPESPGTALYVRPVMFATEAFLGVRPSKTYTLLVIVSPVGAYFSGGAHALRIWVER
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCHHHHHHHHHH
ARARAAQGGIGASKAAANYVASLLAGEEAKAHGYDQVLWLDAARHADLEEIGTMNVFAKL
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECHHCCCHHHHHHHHHHHHH
GATVVTPALEGTILAGITRDCVITLLRDWNVPVEERRVSLAELERAHAAGALEELFGTGT
CHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVVAPIGELAWDGRQLVLPAPGPQSVGERLRAAVHAVQRGEAPDPHGWLEPV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GIPVERTRSPKPHPADADLGFGRHFTDHLFRIDWSEGKGWHAPRVEPYRPLSIDPAASV
CCCCCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
LHYGQAIFEGLKAFRHRDGGMRLFRPEANLARLAASARRLSMPAPEPGIVLEGIRALLRT
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
DAGWLPESPGTALYVRPVMFATEAFLGVRPSKTYTLLVIVSPVGAYFSGGAHALRIWVER
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCHHHHHHHHHH
ARARAAQGGIGASKAAANYVASLLAGEEAKAHGYDQVLWLDAARHADLEEIGTMNVFAKL
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEEEEECHHCCCHHHHHHHHHHHHH
GATVVTPALEGTILAGITRDCVITLLRDWNVPVEERRVSLAELERAHAAGALEELFGTGT
CHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVVAPIGELAWDGRQLVLPAPGPQSVGERLRAAVHAVQRGEAPDPHGWLEPV
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]