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The map label for this gene is zraS [H]

Identifier: 220918622

GI number: 220918622

Start: 3946042

End: 3947688

Strand: Direct

Name: zraS [H]

Synonym: A2cp1_3530

Alternate gene names: 220918622

Gene position: 3946042-3947688 (Clockwise)

Preceding gene: 220918621

Following gene: 220918624

Centisome position: 78.46

GC content: 74.13

Gene sequence:

>1647_bases
GTGCCGTTCTGGCGCCGCCTCTCCACCAAGCTGCTGGCCGCGGTGGTGCTGCTCGCCCTGCTCGCGCTCGCGGCCGTGGT
GCTCGCGGAGCGCCGCTTCGAGCGGCACCTCCAGGACGAGATCGTCCGCTCCACCGCGCTGGTCGGCGAGGCGGTGACCA
CGGTGGGCCAGGCGGCGCTGCTCGGCGCGACCGAGCGCCACGAGTACCGGACCATCGGCCGGCTCGCCGCGCTGGAGGGC
GTGGACCGGATGCGCGTCCTCGACCCCCGGGGCACGGTTCGCTACGCCAGCCGGCCCGAGGAGATCGGGCAGACCCGCGA
CAAGGCGCACGCCTCCTGCGTGCCCTGCCACGCCGGGCACGAGCTGCCGCTCTCGCGGGCGCCGGCGGAGCAGCGCTGGC
GCGAGGAGCGCGGGCCGTCCGGCCGTCGCCTGGTGGTCACCACGCCCGTCTACAACGACCGGACCTGCGCCACCGCCGAG
TGCCACGTCCACCCGCCCGGCCGGCAGGTGCTGGGCCTGCTCGAGACCGACGTGGCCCTGGAGCCGATCCTCTCCGGCGT
GGCGTCGTTCCGGCGCGGCTTCGTCTCGCTGCTCGTGATCGCGATCGTCGCGACCTCGGCGCTCATGTACCTGTTCCTCC
GCTCGGAGGTGCTCGGGCCGGTGGCCGCGCTGGTCGAGGGCACGCGGCGCGTGGCGAAGGACCAGCTCGACGTGGAGGTC
CGGGTCCGCTCGCGCGGCGAGCTCGGCAACCTGGCCGCGTCGTTCAACGACATGACCCGCTCGCTCCGGCGCCTGGAGGA
CGAGCTGAACGGGCTGCTCGCCGGCCTCGAGGAGCAGGTGGAGGCGCGCACGGCCGATCTGCGCAACGCGCAGGAGCAGC
TGGTGCGGACCGAGAAGCTCTCCTCCCTGGGCAAGCTCTCGGCCTCCATCGCCCACGAGATCAACAACCCGCTCGCCGGC
ATCCTCACGTTCGCGAAGCTGGTGTCACGCACCCTGGCCGAGGGTCCGCCCGACGACGCGCGGCGCGCGGTGCTGCAGCG
GAACCTGGGGCTGGTCGAGCGCGAGGCCCAGCGCTGCAGCGCCATCGTGCGCAACCTGCTCGACTTCGCGCGCGAGCGCC
CCATGGCGAAGAAGCCGGTGGATCCGCGCGCGGCGGTGGAGGAGGCGCTGTCCCTCATCGCGAACCAGATCGCCATCCAG
GGCGTGAAGCTGGAGCGCCACCTGCCTCCGCTCCCGCAGGTGCTCGCCGACTTCGGCCAGCTCCGCCAGGCGTTCGTGAA
CGTGGCGATGAACGCCTGCGAGGCGATGGGCGCGAAGGGCGGCAAGCTGCGGATCACCGCGCGCGCGCCGGAGGGAGCGG
TGGAGATCGCGTTCCAGGACGACGGGCCGGGCATGCCGCCGGAGCGCGTGAGCCACATCTTCGACCCGTTCTTCACCACC
AAGGAGAAGGGCACCGGCCTGGGCCTCTCGGTGGTGTACGGCATCGTGCAGCGCCACGGTGGGAGCATCGCCGTGGACAG
CACCGAGGGCGAGGGCACCACCTTCACCTTCCGGCTGCCGGCGGTCCCGGCGGAGCGGACCGCGGAGCCGCCGGCCCCGC
CGATCCCGGCCGACCCGGCCGCCGCGCCGGCGCGCGCGCAGGGCTGA

Upstream 100 bases:

>100_bases
CAAGGCGATCATGGCGGAAGCACGCGAAGCCGAGGAGCGTGGCACCGGCGCCCCGGCGCCGGCCGGTGGCGTCCCGGGAG
GGAGTGCGTCCGGCCGCGCA

Downstream 100 bases:

>100_bases
TCGGCCCGGGCCGGCTCACCCGCCCCGCTGCGCGCCGCGCAGGAGCGCGGCGGCGAGCGAGAGCGGGCCGGAGGCGATGT
CGTCGGCGTCGATCACGATC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MPFWRRLSTKLLAAVVLLALLALAAVVLAERRFERHLQDEIVRSTALVGEAVTTVGQAALLGATERHEYRTIGRLAALEG
VDRMRVLDPRGTVRYASRPEEIGQTRDKAHASCVPCHAGHELPLSRAPAEQRWREERGPSGRRLVVTTPVYNDRTCATAE
CHVHPPGRQVLGLLETDVALEPILSGVASFRRGFVSLLVIAIVATSALMYLFLRSEVLGPVAALVEGTRRVAKDQLDVEV
RVRSRGELGNLAASFNDMTRSLRRLEDELNGLLAGLEEQVEARTADLRNAQEQLVRTEKLSSLGKLSASIAHEINNPLAG
ILTFAKLVSRTLAEGPPDDARRAVLQRNLGLVEREAQRCSAIVRNLLDFARERPMAKKPVDPRAAVEEALSLIANQIAIQ
GVKLERHLPPLPQVLADFGQLRQAFVNVAMNACEAMGAKGGKLRITARAPEGAVEIAFQDDGPGMPPERVSHIFDPFFTT
KEKGTGLGLSVVYGIVQRHGGSIAVDSTEGEGTTFTFRLPAVPAERTAEPPAPPIPADPAAAPARAQG

Sequences:

>Translated_548_residues
MPFWRRLSTKLLAAVVLLALLALAAVVLAERRFERHLQDEIVRSTALVGEAVTTVGQAALLGATERHEYRTIGRLAALEG
VDRMRVLDPRGTVRYASRPEEIGQTRDKAHASCVPCHAGHELPLSRAPAEQRWREERGPSGRRLVVTTPVYNDRTCATAE
CHVHPPGRQVLGLLETDVALEPILSGVASFRRGFVSLLVIAIVATSALMYLFLRSEVLGPVAALVEGTRRVAKDQLDVEV
RVRSRGELGNLAASFNDMTRSLRRLEDELNGLLAGLEEQVEARTADLRNAQEQLVRTEKLSSLGKLSASIAHEINNPLAG
ILTFAKLVSRTLAEGPPDDARRAVLQRNLGLVEREAQRCSAIVRNLLDFARERPMAKKPVDPRAAVEEALSLIANQIAIQ
GVKLERHLPPLPQVLADFGQLRQAFVNVAMNACEAMGAKGGKLRITARAPEGAVEIAFQDDGPGMPPERVSHIFDPFFTT
KEKGTGLGLSVVYGIVQRHGGSIAVDSTEGEGTTFTFRLPAVPAERTAEPPAPPIPADPAAAPARAQG
>Mature_547_residues
PFWRRLSTKLLAAVVLLALLALAAVVLAERRFERHLQDEIVRSTALVGEAVTTVGQAALLGATERHEYRTIGRLAALEGV
DRMRVLDPRGTVRYASRPEEIGQTRDKAHASCVPCHAGHELPLSRAPAEQRWREERGPSGRRLVVTTPVYNDRTCATAEC
HVHPPGRQVLGLLETDVALEPILSGVASFRRGFVSLLVIAIVATSALMYLFLRSEVLGPVAALVEGTRRVAKDQLDVEVR
VRSRGELGNLAASFNDMTRSLRRLEDELNGLLAGLEEQVEARTADLRNAQEQLVRTEKLSSLGKLSASIAHEINNPLAGI
LTFAKLVSRTLAEGPPDDARRAVLQRNLGLVEREAQRCSAIVRNLLDFARERPMAKKPVDPRAAVEEALSLIANQIAIQG
VKLERHLPPLPQVLADFGQLRQAFVNVAMNACEAMGAKGGKLRITARAPEGAVEIAFQDDGPGMPPERVSHIFDPFFTTK
EKGTGLGLSVVYGIVQRHGGSIAVDSTEGEGTTFTFRLPAVPAERTAEPPAPPIPADPAAAPARAQG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=36.7965367965368, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=33.6099585062241, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789149, Length=373, Percent_Identity=26.2734584450402, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081816, Length=366, Percent_Identity=27.0491803278689, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790300, Length=246, Percent_Identity=30.4878048780488, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788393, Length=314, Percent_Identity=26.7515923566879, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI48994928, Length=248, Percent_Identity=27.0161290322581, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790567, Length=95, Percent_Identity=40, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=24.6636771300448, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=27.1551724137931, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=28.7610619469027, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=236, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=403, Percent_Identity=23.3250620347395, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59353; Mature: 59222

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFWRRLSTKLLAAVVLLALLALAAVVLAERRFERHLQDEIVRSTALVGEAVTTVGQAAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGATERHEYRTIGRLAALEGVDRMRVLDPRGTVRYASRPEEIGQTRDKAHASCVPCHAGH
HCCHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCC
ELPLSRAPAEQRWREERGPSGRRLVVTTPVYNDRTCATAECHVHPPGRQVLGLLETDVAL
CCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
EPILSGVASFRRGFVSLLVIAIVATSALMYLFLRSEVLGPVAALVEGTRRVAKDQLDVEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RVRSRGELGNLAASFNDMTRSLRRLEDELNGLLAGLEEQVEARTADLRNAQEQLVRTEKL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLGKLSASIAHEINNPLAGILTFAKLVSRTLAEGPPDDARRAVLQRNLGLVEREAQRCS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHH
AIVRNLLDFARERPMAKKPVDPRAAVEEALSLIANQIAIQGVKLERHLPPLPQVLADFGQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHCCCCHHHHHHHHHH
LRQAFVNVAMNACEAMGAKGGKLRITARAPEGAVEIAFQDDGPGMPPERVSHIFDPFFTT
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KEKGTGLGLSVVYGIVQRHGGSIAVDSTEGEGTTFTFRLPAVPAERTAEPPAPPIPADPA
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHCCCCCCCCCCCCCCC
AAPARAQG
CCCCCCCC
>Mature Secondary Structure 
PFWRRLSTKLLAAVVLLALLALAAVVLAERRFERHLQDEIVRSTALVGEAVTTVGQAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGATERHEYRTIGRLAALEGVDRMRVLDPRGTVRYASRPEEIGQTRDKAHASCVPCHAGH
HCCHHHHHHHHHHHHHHHHCCCHHEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCC
ELPLSRAPAEQRWREERGPSGRRLVVTTPVYNDRTCATAECHVHPPGRQVLGLLETDVAL
CCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
EPILSGVASFRRGFVSLLVIAIVATSALMYLFLRSEVLGPVAALVEGTRRVAKDQLDVEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RVRSRGELGNLAASFNDMTRSLRRLEDELNGLLAGLEEQVEARTADLRNAQEQLVRTEKL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLGKLSASIAHEINNPLAGILTFAKLVSRTLAEGPPDDARRAVLQRNLGLVEREAQRCS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHH
AIVRNLLDFARERPMAKKPVDPRAAVEEALSLIANQIAIQGVKLERHLPPLPQVLADFGQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHCCCCHHHHHHHHHH
LRQAFVNVAMNACEAMGAKGGKLRITARAPEGAVEIAFQDDGPGMPPERVSHIFDPFFTT
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KEKGTGLGLSVVYGIVQRHGGSIAVDSTEGEGTTFTFRLPAVPAERTAEPPAPPIPADPA
CCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCHHCCCCCCCCCCCCCCC
AAPARAQG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]