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The map label for this gene is pcrA [H]

Identifier: 220918612

GI number: 220918612

Start: 3933994

End: 3936009

Strand: Reverse

Name: pcrA [H]

Synonym: A2cp1_3520

Alternate gene names: 220918612

Gene position: 3936009-3933994 (Counterclockwise)

Preceding gene: 220918614

Following gene: 220918610

Centisome position: 78.26

GC content: 73.76

Gene sequence:

>2016_bases
ATGCTCGACCTGTCCACCCTCAACCCGCCGCAGCGCGAGGCGGTGATGACCACCGAGGGCCCGCTGCTGGTGCTCGCGGG
CGCGGGCTCGGGCAAGACCCGCGTCATCGCGCACCGCGTCGCCTACCTGCTGGTGCAGGGCGTGGACCCGGAGCAGGTGC
TCGCGGTCACGTTCACCAACAAGGCCGCGGGCGAGATGCGCGAGCGGGTGGCCGCGCTGGCGGGCCCGCCCGGCGTGGAC
GTGTTCGTCTCCACGTTCCACAGCTTCGGCCTGTGGCTGCTGCAGCAGGAGCACGAGGCGGCCGGGCTGCCCCGCCGCTT
CGCCATCTGCGACGCCGGGGACCAGCTCGCGCTCGTGAAGCGGTGCATGCGCGAGGTGAGCGTGGACGACCGCAAGTTCG
ACGCGCACCGGGTGCTCTCGCTCCTCTCGCGCGCCAAGAACGCCGGCAAGAAGCGGATCGTGCCGCGGGAGGCGGGGCAG
GGCGACGACTACGACCTCGTCGCCGCCGAGGTGTACCCGCGCTACGAGAAGGCGCTCCGCGCCATGCGCTCGGTGGACTT
CGACGACCTCATCGCCCGGCCGGTGGCGATGCTGGCGCACGACCCGGCGCTGCGCGCCCGCTGCCAGGAGCGCTTCCGCT
ACCTGCTGGTGGACGAGTACCAGGACACGAACGCCGCCCAGCTCGAGCTCTTGAAGCTCCTCGCCGGCGAGCGGCGGAAC
GTGTGCGCGGTCGGCGACGACGACCAGGCCATCTACGGCTGGCGCGGCGCCGAGGTGAAGAACATCCTGCGGTTCGAGCG
GCACTTCCCCGGCGCGAAGGAGGTCCGGCTCGAGCAGAACTACCGCTCCACCGGCCACATCCTCGCCTGCGCGAACGCGG
TCATCGCGAAGAACGCGGCGCGCAAGGCGAAGGCGCTGTTCACCGCGGGCGAGGCGGGCGAGCCGGTGCGGGTGGTGGCC
ATGTCCGAGGAGGAGGACGAGGCGCGCTTCGTGGCCGAGGAGATCGCCCGGCAGCGCGGCGAGGGCCGGCCGTGGAGCCA
CTTCGCGGTGCTGTTCCGCCTCAACGCGCTCTCGCGCCCGTTCGAGGAGGCGTTCCGCGAGGGCTCCATGCCGTACAAGG
TGCACGGCGGCCCCGCCTTCTTCGACCGCTCCGAGGTGCGCGACCTGCTCGCGTACCTGAAGCTCTGCGTGCAGCCCGAG
GACGACGTGTCGCTCTCGCGCGTCGTGAACGTGCCGCCGCGCGGGATCGGCGACACGTCCATGGAGCGGGTGCACGACTG
GGCCATCCCGCGGAAGCTCCACCTGCTCGAGGCGATGCGGCGGGCGGCGGAGATCCCGGACCTCCCGCGCGGCGCCGCGG
AGAAGATCGGCGCGTTCGTCGCGCTGGTGGACCGCTTCCAGGCCCGCTTCCGCGAGGGGCCGGTGTCGGAGGCCGCGCGC
GCGCTGGTGGCGGAGGTGGACCTGTTCAGCCACGTGCGCGCCGGGGTGCAGTCGCTCGAGGCGGGATCCCGCCGGGTGGA
CGCGCTGGAGGGGCTGCTGCGCTCGCTCGACGGCTACGTCCAGCGCACCGCGCGCCCGTCGCTCGCCACCTGGCTGCAGC
GGCTCGCGCTCGACTCGCGCGAGGAGGAGGATCCGTCGGGCGAGGGCGGGATCACGCTCATGACGCTGCACGCGGCGAAG
GGGCTCGAGTTCCCGGTGGTGTTCCTGGTGGGCGTCGAGGAGGACTACCTCCCCTGCGCCGGGATCCAGGGCGAGGCCCG
CGACCTCGACGAGGAGCGGCGGCTCGCCTACGTGGGCATCACCCGCGCGCGCGAGCGGCTGTACCTGACGCGCGTCGCGG
CGCGATCGAAGCGCGGCAAGCTCCTGCCCCGCACCCCGTCGCGCTTCCTCGACGACCTGCCGCCCGCGGCCCACGAGAAG
GTGGACCCGGCCGCGCTCGCCGCGCCGCCGCAGGACGTGGCGGCGCACACCGAGAGCGTCATGGCCGCGCTGCGCGCGCG
GCTGGGCGGCCGCTGA

Upstream 100 bases:

>100_bases
ACGGTTGCGCGCCGCGCGACGGCGCGGGCGCGCGGTCGCGCCGGGATTTTCAAGTCTTGCGGGCGGCCCGCCGGCGTGCC
AGATACGCGGGCCCCGCGCC

Downstream 100 bases:

>100_bases
CCGTCGGGGCGCGCCGGCTCACGCGCTCTCAGAGCGCGTGAATCCATCCCCGGACCGAGCCAGGCGGTCGAGACGCGAGC
AGCGCGAGGCGCGACGACCG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MLDLSTLNPPQREAVMTTEGPLLVLAGAGSGKTRVIAHRVAYLLVQGVDPEQVLAVTFTNKAAGEMRERVAALAGPPGVD
VFVSTFHSFGLWLLQQEHEAAGLPRRFAICDAGDQLALVKRCMREVSVDDRKFDAHRVLSLLSRAKNAGKKRIVPREAGQ
GDDYDLVAAEVYPRYEKALRAMRSVDFDDLIARPVAMLAHDPALRARCQERFRYLLVDEYQDTNAAQLELLKLLAGERRN
VCAVGDDDQAIYGWRGAEVKNILRFERHFPGAKEVRLEQNYRSTGHILACANAVIAKNAARKAKALFTAGEAGEPVRVVA
MSEEEDEARFVAEEIARQRGEGRPWSHFAVLFRLNALSRPFEEAFREGSMPYKVHGGPAFFDRSEVRDLLAYLKLCVQPE
DDVSLSRVVNVPPRGIGDTSMERVHDWAIPRKLHLLEAMRRAAEIPDLPRGAAEKIGAFVALVDRFQARFREGPVSEAAR
ALVAEVDLFSHVRAGVQSLEAGSRRVDALEGLLRSLDGYVQRTARPSLATWLQRLALDSREEEDPSGEGGITLMTLHAAK
GLEFPVVFLVGVEEDYLPCAGIQGEARDLDEERRLAYVGITRARERLYLTRVAARSKRGKLLPRTPSRFLDDLPPAAHEK
VDPAALAAPPQDVAAHTESVMAALRARLGGR

Sequences:

>Translated_671_residues
MLDLSTLNPPQREAVMTTEGPLLVLAGAGSGKTRVIAHRVAYLLVQGVDPEQVLAVTFTNKAAGEMRERVAALAGPPGVD
VFVSTFHSFGLWLLQQEHEAAGLPRRFAICDAGDQLALVKRCMREVSVDDRKFDAHRVLSLLSRAKNAGKKRIVPREAGQ
GDDYDLVAAEVYPRYEKALRAMRSVDFDDLIARPVAMLAHDPALRARCQERFRYLLVDEYQDTNAAQLELLKLLAGERRN
VCAVGDDDQAIYGWRGAEVKNILRFERHFPGAKEVRLEQNYRSTGHILACANAVIAKNAARKAKALFTAGEAGEPVRVVA
MSEEEDEARFVAEEIARQRGEGRPWSHFAVLFRLNALSRPFEEAFREGSMPYKVHGGPAFFDRSEVRDLLAYLKLCVQPE
DDVSLSRVVNVPPRGIGDTSMERVHDWAIPRKLHLLEAMRRAAEIPDLPRGAAEKIGAFVALVDRFQARFREGPVSEAAR
ALVAEVDLFSHVRAGVQSLEAGSRRVDALEGLLRSLDGYVQRTARPSLATWLQRLALDSREEEDPSGEGGITLMTLHAAK
GLEFPVVFLVGVEEDYLPCAGIQGEARDLDEERRLAYVGITRARERLYLTRVAARSKRGKLLPRTPSRFLDDLPPAAHEK
VDPAALAAPPQDVAAHTESVMAALRARLGGR
>Mature_671_residues
MLDLSTLNPPQREAVMTTEGPLLVLAGAGSGKTRVIAHRVAYLLVQGVDPEQVLAVTFTNKAAGEMRERVAALAGPPGVD
VFVSTFHSFGLWLLQQEHEAAGLPRRFAICDAGDQLALVKRCMREVSVDDRKFDAHRVLSLLSRAKNAGKKRIVPREAGQ
GDDYDLVAAEVYPRYEKALRAMRSVDFDDLIARPVAMLAHDPALRARCQERFRYLLVDEYQDTNAAQLELLKLLAGERRN
VCAVGDDDQAIYGWRGAEVKNILRFERHFPGAKEVRLEQNYRSTGHILACANAVIAKNAARKAKALFTAGEAGEPVRVVA
MSEEEDEARFVAEEIARQRGEGRPWSHFAVLFRLNALSRPFEEAFREGSMPYKVHGGPAFFDRSEVRDLLAYLKLCVQPE
DDVSLSRVVNVPPRGIGDTSMERVHDWAIPRKLHLLEAMRRAAEIPDLPRGAAEKIGAFVALVDRFQARFREGPVSEAAR
ALVAEVDLFSHVRAGVQSLEAGSRRVDALEGLLRSLDGYVQRTARPSLATWLQRLALDSREEEDPSGEGGITLMTLHAAK
GLEFPVVFLVGVEEDYLPCAGIQGEARDLDEERRLAYVGITRARERLYLTRVAARSKRGKLLPRTPSRFLDDLPPAAHEK
VDPAALAAPPQDVAAHTESVMAALRARLGGR

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=652, Percent_Identity=39.8773006134969, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=39.4984326018809, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=28.8235294117647, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=503, Percent_Identity=28.4294234592445, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74224; Mature: 74224

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSTLNPPQREAVMTTEGPLLVLAGAGSGKTRVIAHRVAYLLVQGVDPEQVLAVTFTN
CCCCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECC
KAAGEMRERVAALAGPPGVDVFVSTFHSFGLWLLQQEHEAAGLPRRFAICDAGDQLALVK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCHHHHHH
RCMREVSVDDRKFDAHRVLSLLSRAKNAGKKRIVPREAGQGDDYDLVAAEVYPRYEKALR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
AMRSVDFDDLIARPVAMLAHDPALRARCQERFRYLLVDEYQDTNAAQLELLKLLAGERRN
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEHHHCCCCCCCHHHHHHHHHHHCCCCC
VCAVGDDDQAIYGWRGAEVKNILRFERHFPGAKEVRLEQNYRSTGHILACANAVIAKNAA
EEEECCCCCEEECCCCHHHHHHHHHHHHCCCHHHEEEHHCCCCCCCCHHHHHHHHHHHHH
RKAKALFTAGEAGEPVRVVAMSEEEDEARFVAEEIARQRGEGRPWSHFAVLFRLNALSRP
HHHHHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
FEEAFREGSMPYKVHGGPAFFDRSEVRDLLAYLKLCVQPEDDVSLSRVVNVPPRGIGDTS
HHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCHH
MERVHDWAIPRKLHLLEAMRRAAEIPDLPRGAAEKIGAFVALVDRFQARFREGPVSEAAR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALVAEVDLFSHVRAGVQSLEAGSRRVDALEGLLRSLDGYVQRTARPSLATWLQRLALDSR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EEEDPSGEGGITLMTLHAAKGLEFPVVFLVGVEEDYLPCAGIQGEARDLDEERRLAYVGI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCHHHCEEEEEH
TRARERLYLTRVAARSKRGKLLPRTPSRFLDDLPPAAHEKVDPAALAAPPQDVAAHTESV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHCCCCCHHHCCCCHHHHHHHHHH
MAALRARLGGR
HHHHHHHHCCC
>Mature Secondary Structure
MLDLSTLNPPQREAVMTTEGPLLVLAGAGSGKTRVIAHRVAYLLVQGVDPEQVLAVTFTN
CCCCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECC
KAAGEMRERVAALAGPPGVDVFVSTFHSFGLWLLQQEHEAAGLPRRFAICDAGDQLALVK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCHHHHHH
RCMREVSVDDRKFDAHRVLSLLSRAKNAGKKRIVPREAGQGDDYDLVAAEVYPRYEKALR
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHH
AMRSVDFDDLIARPVAMLAHDPALRARCQERFRYLLVDEYQDTNAAQLELLKLLAGERRN
HHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEHHHCCCCCCCHHHHHHHHHHHCCCCC
VCAVGDDDQAIYGWRGAEVKNILRFERHFPGAKEVRLEQNYRSTGHILACANAVIAKNAA
EEEECCCCCEEECCCCHHHHHHHHHHHHCCCHHHEEEHHCCCCCCCCHHHHHHHHHHHHH
RKAKALFTAGEAGEPVRVVAMSEEEDEARFVAEEIARQRGEGRPWSHFAVLFRLNALSRP
HHHHHHEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
FEEAFREGSMPYKVHGGPAFFDRSEVRDLLAYLKLCVQPEDDVSLSRVVNVPPRGIGDTS
HHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCCHH
MERVHDWAIPRKLHLLEAMRRAAEIPDLPRGAAEKIGAFVALVDRFQARFREGPVSEAAR
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALVAEVDLFSHVRAGVQSLEAGSRRVDALEGLLRSLDGYVQRTARPSLATWLQRLALDSR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EEEDPSGEGGITLMTLHAAKGLEFPVVFLVGVEEDYLPCAGIQGEARDLDEERRLAYVGI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCHHHCEEEEEH
TRARERLYLTRVAARSKRGKLLPRTPSRFLDDLPPAAHEKVDPAALAAPPQDVAAHTESV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHCCCCCHHHCCCCHHHHHHHHHH
MAALRARLGGR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]