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The map label for this gene is pilT [H]

Identifier: 220918606

GI number: 220918606

Start: 3927704

End: 3928987

Strand: Reverse

Name: pilT [H]

Synonym: A2cp1_3514

Alternate gene names: 220918606

Gene position: 3928987-3927704 (Counterclockwise)

Preceding gene: 220918607

Following gene: 220918605

Centisome position: 78.12

GC content: 70.87

Gene sequence:

>1284_bases
ATGGAACTCAACGAGATCCTGCAGGTCGCGCTCCGCGCCGGCGCCTCGGACATCCACCTGAAGGCGGGCCTCCCGCCGAT
GTTCCGCGTCGACGGCTCGCTGGTGCCGCTGAAGGACGCCCGCCGCCTGCCGCCCGAGGAGATCGCGCGGATGGGCTTCG
GGATCATGAACGAGTACCAGAAGGAGAAGTTCAAGCAGACGAACGAGGTGGACCTCGCCTACGGCGTGCCCGGCCTCGGC
CGCTTCCGCGTGAACGTCTTCCAGCAGCGCGGCACGCTCGGCGTGGTGCTCCGCGTCATCCCGTTCCGCATCCAGAGCAT
CGAGCAGCTCATGCTGCCCAAGATCCTGGAGAAGGTCGCGGGCGAGCAGCGCGGCCTGGTGCTCGTCACCGGCACGACCG
GCTCCGGCAAGTCCACCACGCTCGCGGCGATGATCGACCACATCAACGCGACCGAGACCTGCCACATCATGACCATCGAG
GATCCCATCGAGTTCCTCATCCGCGACAAGCGCTCGATCGTGAACCAGCGCGAGGTCGGGGTGGACACGATGAGCTTCGG
GCAGGCGCTGAAGAGCGCGCTCCGGCAGGACCCCGACGTCATCCTGGTGGGCGAGATGCGCGACCTCGAGACCATCGAGA
CCGCGCTCACCGCGGCCGAGACCGGCCACCTGGTGATGTCCACCCTGCACACGCTCGACGCGACCGAGACCATCAACCGC
ATCATCTCCGCGTTCCCGCCGTACCAGCAGAAGCAGGTCCGCCTGCAGCTCGGCTCGGTGCTGAAGGCGGTGATCTCGCA
GCGCCTGGTGCCGCGCGCCGACGGGAAGGGGCGCGTGCCGGCCATCGAGGTGCTGCTCGCGACGGGGCGCGTCCGCGAGC
TCATCGAGGACAAGGACCGGACGAAGGAGATCACCGACGCCATCTCGCAGGGCCACACCACCTACGGGATGCAGACGTTC
GATCAGTCGCTCATGGCGCTGCTCCGCTCGAGCCTCATCAGCTACGAGGAGGCGCTCCGGCAGGCCACCAACCCGGACGA
CTTCGCGCTGCGCGTCTCGGGCGTGAGCGGCACCTCCGACTCGAAGTGGGACTCCTTCGAGAAGCCGGCCGAGGCGGGCC
CCGCGGCCCGCGCGCCGGCGCCCGGGCAGCCCGCCGCGCCCCGCCCCGCGCCCGGCGCGCCGCTCGGGCGGCTCGCGCCC
GGCGCCGCTCCCCGGCCCCCCGCCGCGCCCCGCGCCGGCGGCCAGGGCGGGCCGGACGACGACTTCCAGATCGAGCGCTT
CTAG

Upstream 100 bases:

>100_bases
CGGCGGCCGGGCGCCGCTCACGCGCGCCTACATAGGCGCCGCGCGCCGGGGTCCGTCCGATTGGATCCGCCGGATCACGA
ATGTTACCCTCGGCACCGCG

Downstream 100 bases:

>100_bases
CGCCGCGCCCCGTGCCGCCCCCCGGGCAGCCACCGCTCGCGCGCGCCAAGGCGATCGCGCTCCGCCAGCTCGCGTCCCGC
ATGCGCACCGAGGCGCAGGT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MELNEILQVALRAGASDIHLKAGLPPMFRVDGSLVPLKDARRLPPEEIARMGFGIMNEYQKEKFKQTNEVDLAYGVPGLG
RFRVNVFQQRGTLGVVLRVIPFRIQSIEQLMLPKILEKVAGEQRGLVLVTGTTGSGKSTTLAAMIDHINATETCHIMTIE
DPIEFLIRDKRSIVNQREVGVDTMSFGQALKSALRQDPDVILVGEMRDLETIETALTAAETGHLVMSTLHTLDATETINR
IISAFPPYQQKQVRLQLGSVLKAVISQRLVPRADGKGRVPAIEVLLATGRVRELIEDKDRTKEITDAISQGHTTYGMQTF
DQSLMALLRSSLISYEEALRQATNPDDFALRVSGVSGTSDSKWDSFEKPAEAGPAARAPAPGQPAAPRPAPGAPLGRLAP
GAAPRPPAAPRAGGQGGPDDDFQIERF

Sequences:

>Translated_427_residues
MELNEILQVALRAGASDIHLKAGLPPMFRVDGSLVPLKDARRLPPEEIARMGFGIMNEYQKEKFKQTNEVDLAYGVPGLG
RFRVNVFQQRGTLGVVLRVIPFRIQSIEQLMLPKILEKVAGEQRGLVLVTGTTGSGKSTTLAAMIDHINATETCHIMTIE
DPIEFLIRDKRSIVNQREVGVDTMSFGQALKSALRQDPDVILVGEMRDLETIETALTAAETGHLVMSTLHTLDATETINR
IISAFPPYQQKQVRLQLGSVLKAVISQRLVPRADGKGRVPAIEVLLATGRVRELIEDKDRTKEITDAISQGHTTYGMQTF
DQSLMALLRSSLISYEEALRQATNPDDFALRVSGVSGTSDSKWDSFEKPAEAGPAARAPAPGQPAAPRPAPGAPLGRLAP
GAAPRPPAAPRAGGQGGPDDDFQIERF
>Mature_427_residues
MELNEILQVALRAGASDIHLKAGLPPMFRVDGSLVPLKDARRLPPEEIARMGFGIMNEYQKEKFKQTNEVDLAYGVPGLG
RFRVNVFQQRGTLGVVLRVIPFRIQSIEQLMLPKILEKVAGEQRGLVLVTGTTGSGKSTTLAAMIDHINATETCHIMTIE
DPIEFLIRDKRSIVNQREVGVDTMSFGQALKSALRQDPDVILVGEMRDLETIETALTAAETGHLVMSTLHTLDATETINR
IISAFPPYQQKQVRLQLGSVLKAVISQRLVPRADGKGRVPAIEVLLATGRVRELIEDKDRTKEITDAISQGHTTYGMQTF
DQSLMALLRSSLISYEEALRQATNPDDFALRVSGVSGTSDSKWDSFEKPAEAGPAARAPAPGQPAAPRPAPGAPLGRLAP
GAAPRPPAAPRAGGQGGPDDDFQIERF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=323, Percent_Identity=38.6996904024768, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789723, Length=288, Percent_Identity=35.4166666666667, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786296, Length=290, Percent_Identity=36.2068965517241, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 46405; Mature: 46405

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNEILQVALRAGASDIHLKAGLPPMFRVDGSLVPLKDARRLPPEEIARMGFGIMNEYQ
CCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCEECCHHHHCCCHHHHHHHHHHHHHHHH
KEKFKQTNEVDLAYGVPGLGRFRVNVFQQRGTLGVVLRVIPFRIQSIEQLMLPKILEKVA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEQRGLVLVTGTTGSGKSTTLAAMIDHINATETCHIMTIEDPIEFLIRDKRSIVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCC
VDTMSFGQALKSALRQDPDVILVGEMRDLETIETALTAAETGHLVMSTLHTLDATETINR
CCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISAFPPYQQKQVRLQLGSVLKAVISQRLVPRADGKGRVPAIEVLLATGRVRELIEDKDR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TKEITDAISQGHTTYGMQTFDQSLMALLRSSLISYEEALRQATNPDDFALRVSGVSGTSD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SKWDSFEKPAEAGPAARAPAPGQPAAPRPAPGAPLGRLAPGAAPRPPAAPRAGGQGGPDD
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFQIERF
CCEECCC
>Mature Secondary Structure
MELNEILQVALRAGASDIHLKAGLPPMFRVDGSLVPLKDARRLPPEEIARMGFGIMNEYQ
CCHHHHHHHHHHCCCCCEEEECCCCCCEECCCCEECCHHHHCCCHHHHHHHHHHHHHHHH
KEKFKQTNEVDLAYGVPGLGRFRVNVFQQRGTLGVVLRVIPFRIQSIEQLMLPKILEKVA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEQRGLVLVTGTTGSGKSTTLAAMIDHINATETCHIMTIEDPIEFLIRDKRSIVNQREVG
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCC
VDTMSFGQALKSALRQDPDVILVGEMRDLETIETALTAAETGHLVMSTLHTLDATETINR
CCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISAFPPYQQKQVRLQLGSVLKAVISQRLVPRADGKGRVPAIEVLLATGRVRELIEDKDR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TKEITDAISQGHTTYGMQTFDQSLMALLRSSLISYEEALRQATNPDDFALRVSGVSGTSD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SKWDSFEKPAEAGPAARAPAPGQPAAPRPAPGAPLGRLAPGAAPRPPAAPRAGGQGGPDD
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFQIERF
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]