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The map label for this gene is leuA [H]

Identifier: 220918332

GI number: 220918332

Start: 3595769

End: 3597112

Strand: Reverse

Name: leuA [H]

Synonym: A2cp1_3236

Alternate gene names: 220918332

Gene position: 3597112-3595769 (Counterclockwise)

Preceding gene: 220918340

Following gene: 220918331

Centisome position: 71.52

GC content: 73.44

Gene sequence:

>1344_bases
ATGCCGAGGATGCCCGCCCACAAGTACCGCCCGTTCCCGCAGGTCGCGCTCGCCGACCGGCGCTGGCCGTCGCGCCGCAT
CGAGCGCGCGCCCCGCTGGTGCTCGGTGGATCTCCGGGACGGGAACCAGGCGCTCGAGGATCCGATGGGCCCGGAGCGCA
AGCGGCTGCTGTTCGACGCCCTGGTGCGCGCGGGGTTCCGGGAGATCGAGGTGGGCTTTCCGTCCGCCTCCCGGCCCGAG
CGGGAGTTCGTCCGCGAGCTCGTCGAACGCGGCATCCCGGAGGACGTGACGCTCCAGGTGCTCACGCAGGCGCGCGAGCA
CCTCGTCCGCGCGACCTTCGATGCGATCCGCGGCGCGCCGCGTGCGGTCGTGCACCTGTACAACTCGACCTCCGAGCTGC
AGCGGCGCGTCGTGTTCGGCAAGGACCGGGCCGGGATCCGGCAGATCGCGGTGGACGGTACGCGGCTCGCGCGCCGGCTG
GCGGAGGAGCTGCCGGAGACCGAGGTGATCCTCGAGTACACGGCGGAGAGCTTCACCGGCACCGAGCTCGACTTCGCCGC
CGACGTCTGCGAGGCGGTGATGGACGCGTGGGGCGCCTCGGCGGAGCGGCCGGTGATCCTCAACCTGCCGGCCACGGTGG
AGATGTCCACGCCGAACGTGTACGCGGACCAGGTCGAGTGGTTCGCGCGCCACGTGCAGGGGCGGGAGCGCTACGTGCTC
TCGGTGCACCCGCACAACGACCGCGGCACCGCGGTGGCGGCGGCGGAGCTGGCGGTGATGGCGGGCGCCGACCGGGTGGA
GGGCACGCTGTTCGGGAACGGCGAGCGCACCGGCAACGTGGACCTGGTGACGCTCGCGCTCAACCTGCACACGCAGGGCG
TCGATCCCGGCCTGGACCTCGCCGACCTCGACGCGCTGGTCGAGGTGGCGGAGCGGGCCACGCGGATGCCGGTGCACCCG
CGCCACCCCTACGCCGGGGCGCTCGTCTACACCGCGTTCTCCGGCTCGCACCAGGACGCGATCAAGAAGGGCTTCGCCGC
GCTCCGCCGCACGAGCGGCGATCGCTGGGAGATGCCCTACCTGCCCATGGACCCGGCGGACGTGGGCCGCACGTACCAGG
CGGTCATCCGGGTGAACAGCCAGTCCGGGAAGGGCGGGGTGGGCTGGGTGCTCGAGGAGCACGGCGGCATCACGCTCGGG
CGCGAGGAGCTGGTCCGCTTCGCGGCGGTGATCCAGGGCATGGCGGAAGCGCGCGGGACGGACCTCACGCCGGCCGAGAT
CCTCGAGGTGTACGCGCGAACGCACGGGGGCGCGCCGGGCGTCCGCGCGATCGCGCAGGGATGA

Upstream 100 bases:

>100_bases
ACCGCGCGACCTCTCTCGCCGTCCCACGGCCGGAGAGGTCACCGCTCCCAGCAAGACGAACAGAGACCTCCCGGCCCCCA
CCCGCCGGAGGAATCACGTC

Downstream 100 bases:

>100_bases
CGCGGCGGAGCGCGGCGCGGGCGGTTATGAGTCGCCCCGCCGCGCCCTCCGGCGCGCCGGAGGCGATCACATGGAGCAGC
GCACGCTGGGGACGCAGGGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPRMPAHKYRPFPQVALADRRWPSRRIERAPRWCSVDLRDGNQALEDPMGPERKRLLFDALVRAGFREIEVGFPSASRPE
REFVRELVERGIPEDVTLQVLTQAREHLVRATFDAIRGAPRAVVHLYNSTSELQRRVVFGKDRAGIRQIAVDGTRLARRL
AEELPETEVILEYTAESFTGTELDFAADVCEAVMDAWGASAERPVILNLPATVEMSTPNVYADQVEWFARHVQGRERYVL
SVHPHNDRGTAVAAAELAVMAGADRVEGTLFGNGERTGNVDLVTLALNLHTQGVDPGLDLADLDALVEVAERATRMPVHP
RHPYAGALVYTAFSGSHQDAIKKGFAALRRTSGDRWEMPYLPMDPADVGRTYQAVIRVNSQSGKGGVGWVLEEHGGITLG
REELVRFAAVIQGMAEARGTDLTPAEILEVYARTHGGAPGVRAIAQG

Sequences:

>Translated_447_residues
MPRMPAHKYRPFPQVALADRRWPSRRIERAPRWCSVDLRDGNQALEDPMGPERKRLLFDALVRAGFREIEVGFPSASRPE
REFVRELVERGIPEDVTLQVLTQAREHLVRATFDAIRGAPRAVVHLYNSTSELQRRVVFGKDRAGIRQIAVDGTRLARRL
AEELPETEVILEYTAESFTGTELDFAADVCEAVMDAWGASAERPVILNLPATVEMSTPNVYADQVEWFARHVQGRERYVL
SVHPHNDRGTAVAAAELAVMAGADRVEGTLFGNGERTGNVDLVTLALNLHTQGVDPGLDLADLDALVEVAERATRMPVHP
RHPYAGALVYTAFSGSHQDAIKKGFAALRRTSGDRWEMPYLPMDPADVGRTYQAVIRVNSQSGKGGVGWVLEEHGGITLG
REELVRFAAVIQGMAEARGTDLTPAEILEVYARTHGGAPGVRAIAQG
>Mature_446_residues
PRMPAHKYRPFPQVALADRRWPSRRIERAPRWCSVDLRDGNQALEDPMGPERKRLLFDALVRAGFREIEVGFPSASRPER
EFVRELVERGIPEDVTLQVLTQAREHLVRATFDAIRGAPRAVVHLYNSTSELQRRVVFGKDRAGIRQIAVDGTRLARRLA
EELPETEVILEYTAESFTGTELDFAADVCEAVMDAWGASAERPVILNLPATVEMSTPNVYADQVEWFARHVQGRERYVLS
VHPHNDRGTAVAAAELAVMAGADRVEGTLFGNGERTGNVDLVTLALNLHTQGVDPGLDLADLDALVEVAERATRMPVHPR
HPYAGALVYTAFSGSHQDAIKKGFAALRRTSGDRWEMPYLPMDPADVGRTYQAVIRVNSQSGKGGVGWVLEEHGGITLGR
EELVRFAAVIQGMAEARGTDLTPAEILEVYARTHGGAPGVRAIAQG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=370, Percent_Identity=26.2162162162162, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324225, Length=442, Percent_Identity=50.4524886877828, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=49.7737556561086, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 49286; Mature: 49155

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRMPAHKYRPFPQVALADRRWPSRRIERAPRWCSVDLRDGNQALEDPMGPERKRLLFDA
CCCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHH
LVRAGFREIEVGFPSASRPEREFVRELVERGIPEDVTLQVLTQAREHLVRATFDAIRGAP
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RAVVHLYNSTSELQRRVVFGKDRAGIRQIAVDGTRLARRLAEELPETEVILEYTAESFTG
HHEEEECCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHCCCCEEEHEEHHCCCCC
TELDFAADVCEAVMDAWGASAERPVILNLPATVEMSTPNVYADQVEWFARHVQGRERYVL
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEEE
SVHPHNDRGTAVAAAELAVMAGADRVEGTLFGNGERTGNVDLVTLALNLHTQGVDPGLDL
EEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCH
ADLDALVEVAERATRMPVHPRHPYAGALVYTAFSGSHQDAIKKGFAALRRTSGDRWEMPY
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
LPMDPADVGRTYQAVIRVNSQSGKGGVGWVLEEHGGITLGREELVRFAAVIQGMAEARGT
CCCCHHHHCHHEEEEEEEECCCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHCCC
DLTPAEILEVYARTHGGAPGVRAIAQG
CCCHHHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
PRMPAHKYRPFPQVALADRRWPSRRIERAPRWCSVDLRDGNQALEDPMGPERKRLLFDA
CCCCCCCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEECCCCHHHHCCCCHHHHHHHHHH
LVRAGFREIEVGFPSASRPEREFVRELVERGIPEDVTLQVLTQAREHLVRATFDAIRGAP
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RAVVHLYNSTSELQRRVVFGKDRAGIRQIAVDGTRLARRLAEELPETEVILEYTAESFTG
HHEEEECCCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHCCCCEEEHEEHHCCCCC
TELDFAADVCEAVMDAWGASAERPVILNLPATVEMSTPNVYADQVEWFARHVQGRERYVL
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEEE
SVHPHNDRGTAVAAAELAVMAGADRVEGTLFGNGERTGNVDLVTLALNLHTQGVDPGLDL
EEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCH
ADLDALVEVAERATRMPVHPRHPYAGALVYTAFSGSHQDAIKKGFAALRRTSGDRWEMPY
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
LPMDPADVGRTYQAVIRVNSQSGKGGVGWVLEEHGGITLGREELVRFAAVIQGMAEARGT
CCCCHHHHCHHEEEEEEEECCCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHCCC
DLTPAEILEVYARTHGGAPGVRAIAQG
CCCHHHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA