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The map label for this gene is macA [H]

Identifier: 220918265

GI number: 220918265

Start: 3508329

End: 3509621

Strand: Reverse

Name: macA [H]

Synonym: A2cp1_3168

Alternate gene names: 220918265

Gene position: 3509621-3508329 (Counterclockwise)

Preceding gene: 220918273

Following gene: 220918264

Centisome position: 69.78

GC content: 76.18

Gene sequence:

>1293_bases
ATGACGCGCCGGAGACTGACGTACGCCGGGGTGGCGATCGCCGCCCTCGCCGCGCTGCTGGGCCTGTGGCGCTGGCGGTC
CGCCAGCAGCGCCGGCGAGGTCCACTACGAGACGGCCAAGGTGGATCGGGGCCGGGTCGTGGCCAAGGTGACCGCCACCG
GGACGCTCTCCGCCCTGGTCACCGTCCAGGTCGGTAGCCAGGTGTCCGGTCGGATCTCGGAGCTGCGCGCGGACTTCAAC
TCGCGGGTCCGCAAGGGCGAGGTGATCGCCCGCATCGACCCGCAGCTGTTCCGGGCCGCCGTGGCGCAGGCGAAGGCGAA
CACCGTGGCCGCCGAGGGCAACCTCGCCAAGGCGCGCGCGCAGGCGGCGGACGCCGACCGGCAGCTGGGGCGGACCCGGC
AGCTCGCCGAGCGCAACCTGGTCGCGGCCGCCGACCTCGACACCGCCCAGGCGAACGCGGACGGCGCGCGCGCCGGGGTG
CAGGCGGCGCAGGGCACGCTGGCCCAGACGCGGGCCGCGCTCCAGCAGGCCCAGGTGAACCTGGGCTACACCGACATCGT
CTCGCCCACCAACGGCGTGGTGATCTCGCGCAACGTGGACGTGGGCCAGACCGTGGCCGCGTCGCTCCAGGCGCCCATCC
TGTTCGTCATCGCGGAGGACCTCGCCAAGATGCAGGTGGACACCAGCGTGGCCGAGGCGGACGTCGGCCGGCTGCGCGCC
GGCATGCCCGCCACCTTCACCGTGGACGCGTATCCGAACGAGGTGTTCTCCGGGACGGTCCGCCAGGTCCGCAACGCCTC
CACCACCGTCCAGAACGTGGTGACCTACGACGCGGTGGTGGACGTGGCCAACCCCGAGCTGAAGCTGAAGCCCGGCATGA
CCGCCACCGTGACGTTCGTGTACGCGCAGCGCGACGACGTGCTCCGCGTACCCAACGCGGCGCTGCGCTTCCGCCCGCCG
CCCGGCCTGAAGCGCCCGGTGCAGGGCGCGCAGGCGCAGGAGGCGGCCGCGCCGGCGGGGCAGGGGGCACGGGCCGCCGC
GCGGCCGGACCGGAGCGGCGGGGACGGCGCGCGCGCGCGGCCGGAGGGCGGCCGGACGGTGTGGGTGCTGCGCGGCGAGG
CGCGGGACCCGGTCCCGGTCGCGGTGCAGACCGGGATCACCGACGGCAGCGCCACCGAGCTCGTCTCCGGCGACCTGCGG
GAGGGCGACGCGGTCGTCACCGACGCGACCGGCGGCGCCGCGAGCGGGCGGCAGGGCGGCGGCGGCGGGGCCATGCGCCG
CGGGCCGTTCTAG

Upstream 100 bases:

>100_bases
CCCCGCGGCGCGCGATGCCGGCCCGCGAAGCACGCGGAGGGTCGCGCGCCGGCAACGACGATGCGGCGCGGCCTCCGGCC
GCCCGGGAGACACGCACACC

Downstream 100 bases:

>100_bases
GGGGCGCGACGTGGCCGAGACCCCGCTCATCCAGCTCGAGGACGTCTGGAAGACGTACGCCATGGGCGACGTCGAGCTGC
ACGCGCTGAAGGGCGTGTCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTRRRLTYAGVAIAALAALLGLWRWRSASSAGEVHYETAKVDRGRVVAKVTATGTLSALVTVQVGSQVSGRISELRADFN
SRVRKGEVIARIDPQLFRAAVAQAKANTVAAEGNLAKARAQAADADRQLGRTRQLAERNLVAAADLDTAQANADGARAGV
QAAQGTLAQTRAALQQAQVNLGYTDIVSPTNGVVISRNVDVGQTVAASLQAPILFVIAEDLAKMQVDTSVAEADVGRLRA
GMPATFTVDAYPNEVFSGTVRQVRNASTTVQNVVTYDAVVDVANPELKLKPGMTATVTFVYAQRDDVLRVPNAALRFRPP
PGLKRPVQGAQAQEAAAPAGQGARAAARPDRSGGDGARARPEGGRTVWVLRGEARDPVPVAVQTGITDGSATELVSGDLR
EGDAVVTDATGGAASGRQGGGGGAMRRGPF

Sequences:

>Translated_430_residues
MTRRRLTYAGVAIAALAALLGLWRWRSASSAGEVHYETAKVDRGRVVAKVTATGTLSALVTVQVGSQVSGRISELRADFN
SRVRKGEVIARIDPQLFRAAVAQAKANTVAAEGNLAKARAQAADADRQLGRTRQLAERNLVAAADLDTAQANADGARAGV
QAAQGTLAQTRAALQQAQVNLGYTDIVSPTNGVVISRNVDVGQTVAASLQAPILFVIAEDLAKMQVDTSVAEADVGRLRA
GMPATFTVDAYPNEVFSGTVRQVRNASTTVQNVVTYDAVVDVANPELKLKPGMTATVTFVYAQRDDVLRVPNAALRFRPP
PGLKRPVQGAQAQEAAAPAGQGARAAARPDRSGGDGARARPEGGRTVWVLRGEARDPVPVAVQTGITDGSATELVSGDLR
EGDAVVTDATGGAASGRQGGGGGAMRRGPF
>Mature_429_residues
TRRRLTYAGVAIAALAALLGLWRWRSASSAGEVHYETAKVDRGRVVAKVTATGTLSALVTVQVGSQVSGRISELRADFNS
RVRKGEVIARIDPQLFRAAVAQAKANTVAAEGNLAKARAQAADADRQLGRTRQLAERNLVAAADLDTAQANADGARAGVQ
AAQGTLAQTRAALQQAQVNLGYTDIVSPTNGVVISRNVDVGQTVAASLQAPILFVIAEDLAKMQVDTSVAEADVGRLRAG
MPATFTVDAYPNEVFSGTVRQVRNASTTVQNVVTYDAVVDVANPELKLKPGMTATVTFVYAQRDDVLRVPNAALRFRPPP
GLKRPVQGAQAQEAAAPAGQGARAAARPDRSGGDGARARPEGGRTVWVLRGEARDPVPVAVQTGITDGSATELVSGDLRE
GDAVVTDATGGAASGRQGGGGGAMRRGPF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=315, Percent_Identity=33.015873015873, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082036, Length=276, Percent_Identity=27.8985507246377, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081951, Length=265, Percent_Identity=29.0566037735849, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790519, Length=241, Percent_Identity=30.2904564315353, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789929, Length=273, Percent_Identity=30.4029304029304, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790024, Length=296, Percent_Identity=25.3378378378378, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789041, Length=232, Percent_Identity=28.0172413793103, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790012, Length=241, Percent_Identity=26.9709543568465, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44644; Mature: 44513

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRRLTYAGVAIAALAALLGLWRWRSASSAGEVHYETAKVDRGRVVAKVTATGTLSALV
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCEEEEE
TVQVGSQVSGRISELRADFNSRVRKGEVIARIDPQLFRAAVAQAKANTVAAEGNLAKARA
EEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCEEEECCCHHHHHH
QAADADRQLGRTRQLAERNLVAAADLDTAQANADGARAGVQAAQGTLAQTRAALQQAQVN
HHHHHHHHHHHHHHHHHHCEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LGYTDIVSPTNGVVISRNVDVGQTVAASLQAPILFVIAEDLAKMQVDTSVAEADVGRLRA
CCCCEECCCCCCEEEEECCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
GMPATFTVDAYPNEVFSGTVRQVRNASTTVQNVVTYDAVVDVANPELKLKPGMTATVTFV
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECCCCEEEEEEE
YAQRDDVLRVPNAALRFRPPPGLKRPVQGAQAQEAAAPAGQGARAAARPDRSGGDGARAR
EECCCCEEECCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PEGGRTVWVLRGEARDPVPVAVQTGITDGSATELVSGDLREGDAVVTDATGGAASGRQGG
CCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
GGGAMRRGPF
CCCCCCCCCC
>Mature Secondary Structure 
TRRRLTYAGVAIAALAALLGLWRWRSASSAGEVHYETAKVDRGRVVAKVTATGTLSALV
CCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEEECCCEEEEE
TVQVGSQVSGRISELRADFNSRVRKGEVIARIDPQLFRAAVAQAKANTVAAEGNLAKARA
EEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCEEEECCCHHHHHH
QAADADRQLGRTRQLAERNLVAAADLDTAQANADGARAGVQAAQGTLAQTRAALQQAQVN
HHHHHHHHHHHHHHHHHHCEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LGYTDIVSPTNGVVISRNVDVGQTVAASLQAPILFVIAEDLAKMQVDTSVAEADVGRLRA
CCCCEECCCCCCEEEEECCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
GMPATFTVDAYPNEVFSGTVRQVRNASTTVQNVVTYDAVVDVANPELKLKPGMTATVTFV
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECCCCEEEEEEE
YAQRDDVLRVPNAALRFRPPPGLKRPVQGAQAQEAAAPAGQGARAAARPDRSGGDGARAR
EECCCCEEECCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PEGGRTVWVLRGEARDPVPVAVQTGITDGSATELVSGDLREGDAVVTDATGGAASGRQGG
CCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
GGGAMRRGPF
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]