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The map label for this gene is thiF [C]

Identifier: 220918218

GI number: 220918218

Start: 3447133

End: 3448947

Strand: Reverse

Name: thiF [C]

Synonym: A2cp1_3121

Alternate gene names: 220918218

Gene position: 3448947-3447133 (Counterclockwise)

Preceding gene: 220918219

Following gene: 220918215

Centisome position: 68.58

GC content: 80.83

Gene sequence:

>1815_bases
GTGTGGTGCGTGGCCGACTGGGTCCTCCGCGCCCTCGACGCGGAGCTCGGCGGCCATCCGCCGGAGCGCGGCGGCGCGCT
GCTCGGTCCGCCGGGGCGGCCGCTCGTCACCCGATTCGAGCCGGATCCGGGGGCGCACGCCAGCGCGAGCCGGTGGGCGC
CGTCGGCCGGGCTGGGGGAGCGGGTCACCGCGCTCGAGCGCGGCGAGGGGCTGGAGCTGAAGGGGCTGGTGCACTCGCAC
CCCGGCGCCCTCGACCAGCCCTCGGCCCAGGACGCGCGCGAGCTCGCCGCCGGGCTCGCGCACAACCCGCACCTCGCCTG
CTATCTGGGCCCGATCGTCTCGCTCGAGCCGCCCGCCGGCCTGGGCGCGCACGAGGTGGCGCTGGCGCGCGGGAAGCTCT
CGCTGTTCGCCGCGCGCCGGAGCCGGGGCGGCGGCGCCGAGGTCGCCCCGGAGCCGCTCCGCGCCATCCCGCTGCTCCGC
GACCTGGAGCGCGCCGCGCAGGCGCTCGGCGGCGGACGCGCCGAGCCGTTCGTCACCGACGCCGGCGAGGGGCCGCTGCT
GGCCGGTCGCCTGCGCCTCGACGGCGGCGGCGAGCTGCTGGTGCTCGCCGGCGAGCACTACCCGGCGCTGCCGCCGGTGG
CGCTCGCCGCCGGCGAGCGCGAGGCCAGCGGCCAGCTCGCGCTCTCGTGGCCGCTCGCGCTCGCCGAGGACGAGCGGCTC
GCCGCCGCGCTCGCGGCGGTGCTGGAGGGGCGCGGCCCGCGCCGGCGCGCGTACGGGCCACCCGGCGGCCCGGCGCTGAC
GCGCGACCCGGGACGAGCACGGCTGGCGGGGTGGACCGCGCGGCTCGCGGACGGCGGCCCGGAGGCGCGCGAGGCGGCGC
GGCGCGGCGGGCTGCGCGCGCGGAGCGCGGCGCGGCTGCCCGAGGCGCTGCGCGAGCGCGCCGTGCTGGTGGCCGGGTGC
GGGTCGGTGGGCTCGTACCTGGCCGAGCTGCTGGCGCGGGCCGGCGTGGGGCGGCTCGCGCTCCTCGATCCGGAGGTGGT
GGAGCCCGCGAACCTCTCGCGCACCGTGTTCGCCGCCGAGGACGTGGGCCGGTCGAAGCCGGAGGCGCTGGCCCGGCGGT
TGCTCGCCGTGGAGCCGTCGATCGCGCTGGCGCTCGAGCCGCGCGCGGTGGACGGGCTCCCGCCGGCGGCGCTGGACGCG
CGGGTGCGCGAGGCGGACCTCGTGCTCGCCGCCACCGACGATCCCGCGGCGCAGCGCGCCCTCGACCGCTTCGCGTACGC
GCGCGGGCGCCCGGCGCTGTTCGTGGGCCTGTACGCGGGCGCGCAGGGCGGCGAGGTGATCCTCACGGTCCCGGGCCGGA
CCGCGTGCTACCTGTGCGCGACCCGGAGCCGCCACGGGCTGGAGCGCGCCGGCGGCCGGGTGGCGCGCGAGCTGGACTAC
GGCACCGGGCGGCTGCGGGGCGAGGTCGCGCTCGCCGCCGACGTGCAGCACGTGGCGAGCGCCGCGGCGAAGCTGGCGCT
GGCGCTGCTGGCGCCGGAGGGCGACGCCGGGCCGGGGCCGTTCGCGGAGCAGGTGATCGCGTCCGGCGCCACCTACCTCA
CGCTCTCGACCGTGCCCGGCTACTGGTTCTACCCGCAGGTGTTCGGTGACACGCCGGGGCAGGGCGCGTACCAGTCGGTG
TGGCTCACGCCGGCGCGCGCCCCGGACTGCCCGGTGTGCGGGCCGCCCGAGGCGCGCTGCGATCCGCTCGAGGTGCCGCG
CCGGACGCCGGACGCCGCGGCCATCGCCCGCGCGCTGCGCGACGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CGGACGGCCGGGTGGTGATCGGCGTGGAGCAGGGCGACGCCGAGCAGCGCATGCTGTCGAGGGTGCACCCGGCGGTGTTC
GCGTAACGGCAGGGCGGCGC

Downstream 100 bases:

>100_bases
GGCCCCGAGCCGCCGGCTAGAACACGTCCTCCGGCGACACGCGCACCGGGACGCCCGCCGCGTCACCCTCGCCGAAGACC
GGCTGGCGCGCCAGCTTCGG

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MWCVADWVLRALDAELGGHPPERGGALLGPPGRPLVTRFEPDPGAHASASRWAPSAGLGERVTALERGEGLELKGLVHSH
PGALDQPSAQDARELAAGLAHNPHLACYLGPIVSLEPPAGLGAHEVALARGKLSLFAARRSRGGGAEVAPEPLRAIPLLR
DLERAAQALGGGRAEPFVTDAGEGPLLAGRLRLDGGGELLVLAGEHYPALPPVALAAGEREASGQLALSWPLALAEDERL
AAALAAVLEGRGPRRRAYGPPGGPALTRDPGRARLAGWTARLADGGPEAREAARRGGLRARSAARLPEALRERAVLVAGC
GSVGSYLAELLARAGVGRLALLDPEVVEPANLSRTVFAAEDVGRSKPEALARRLLAVEPSIALALEPRAVDGLPPAALDA
RVREADLVLAATDDPAAQRALDRFAYARGRPALFVGLYAGAQGGEVILTVPGRTACYLCATRSRHGLERAGGRVARELDY
GTGRLRGEVALAADVQHVASAAAKLALALLAPEGDAGPGPFAEQVIASGATYLTLSTVPGYWFYPQVFGDTPGQGAYQSV
WLTPARAPDCPVCGPPEARCDPLEVPRRTPDAAAIARALRDAGA

Sequences:

>Translated_604_residues
MWCVADWVLRALDAELGGHPPERGGALLGPPGRPLVTRFEPDPGAHASASRWAPSAGLGERVTALERGEGLELKGLVHSH
PGALDQPSAQDARELAAGLAHNPHLACYLGPIVSLEPPAGLGAHEVALARGKLSLFAARRSRGGGAEVAPEPLRAIPLLR
DLERAAQALGGGRAEPFVTDAGEGPLLAGRLRLDGGGELLVLAGEHYPALPPVALAAGEREASGQLALSWPLALAEDERL
AAALAAVLEGRGPRRRAYGPPGGPALTRDPGRARLAGWTARLADGGPEAREAARRGGLRARSAARLPEALRERAVLVAGC
GSVGSYLAELLARAGVGRLALLDPEVVEPANLSRTVFAAEDVGRSKPEALARRLLAVEPSIALALEPRAVDGLPPAALDA
RVREADLVLAATDDPAAQRALDRFAYARGRPALFVGLYAGAQGGEVILTVPGRTACYLCATRSRHGLERAGGRVARELDY
GTGRLRGEVALAADVQHVASAAAKLALALLAPEGDAGPGPFAEQVIASGATYLTLSTVPGYWFYPQVFGDTPGQGAYQSV
WLTPARAPDCPVCGPPEARCDPLEVPRRTPDAAAIARALRDAGA
>Mature_604_residues
MWCVADWVLRALDAELGGHPPERGGALLGPPGRPLVTRFEPDPGAHASASRWAPSAGLGERVTALERGEGLELKGLVHSH
PGALDQPSAQDARELAAGLAHNPHLACYLGPIVSLEPPAGLGAHEVALARGKLSLFAARRSRGGGAEVAPEPLRAIPLLR
DLERAAQALGGGRAEPFVTDAGEGPLLAGRLRLDGGGELLVLAGEHYPALPPVALAAGEREASGQLALSWPLALAEDERL
AAALAAVLEGRGPRRRAYGPPGGPALTRDPGRARLAGWTARLADGGPEAREAARRGGLRARSAARLPEALRERAVLVAGC
GSVGSYLAELLARAGVGRLALLDPEVVEPANLSRTVFAAEDVGRSKPEALARRLLAVEPSIALALEPRAVDGLPPAALDA
RVREADLVLAATDDPAAQRALDRFAYARGRPALFVGLYAGAQGGEVILTVPGRTACYLCATRSRHGLERAGGRVARELDY
GTGRLRGEVALAADVQHVASAAAKLALALLAPEGDAGPGPFAEQVIASGATYLTLSTVPGYWFYPQVFGDTPGQGAYQSV
WLTPARAPDCPVCGPPEARCDPLEVPRRTPDAAAIARALRDAGA

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI13376212, Length=200, Percent_Identity=28.5, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI38327032, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI87082356, Length=154, Percent_Identity=35.7142857142857, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787048, Length=156, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508819, Length=209, Percent_Identity=29.6650717703349, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24641311, Length=208, Percent_Identity=29.8076923076923, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62465; Mature: 62465

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWCVADWVLRALDAELGGHPPERGGALLGPPGRPLVTRFEPDPGAHASASRWAPSAGLGE
CCCHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
RVTALERGEGLELKGLVHSHPGALDQPSAQDARELAAGLAHNPHLACYLGPIVSLEPPAG
HHHHHHCCCCCEEEEEHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCC
LGAHEVALARGKLSLFAARRSRGGGAEVAPEPLRAIPLLRDLERAAQALGGGRAEPFVTD
CCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
AGEGPLLAGRLRLDGGGELLVLAGEHYPALPPVALAAGEREASGQLALSWPLALAEDERL
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHEECCCCCCCCCEEEECCEEECCCHHH
AAALAAVLEGRGPRRRAYGPPGGPALTRDPGRARLAGWTARLADGGPEAREAARRGGLRA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCH
RSAARLPEALRERAVLVAGCGSVGSYLAELLARAGVGRLALLDPEVVEPANLSRTVFAAE
HHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEHH
DVGRSKPEALARRLLAVEPSIALALEPRAVDGLPPAALDARVREADLVLAATDDPAAQRA
HCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHEEECCEEEEECCCHHHHHH
LDRFAYARGRPALFVGLYAGAQGGEVILTVPGRTACYLCATRSRHGLERAGGRVARELDY
HHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCHHHHCCHHHHHHCC
GTGRLRGEVALAADVQHVASAAAKLALALLAPEGDAGPGPFAEQVIASGATYLTLSTVPG
CCCCCCCCEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCC
YWFYPQVFGDTPGQGAYQSVWLTPARAPDCPVCGPPEARCDPLEVPRRTPDAAAIARALR
CEECHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGA
HCCC
>Mature Secondary Structure
MWCVADWVLRALDAELGGHPPERGGALLGPPGRPLVTRFEPDPGAHASASRWAPSAGLGE
CCCHHHHHHHHHHHHHCCCCCHHCCEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
RVTALERGEGLELKGLVHSHPGALDQPSAQDARELAAGLAHNPHLACYLGPIVSLEPPAG
HHHHHHCCCCCEEEEEHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCC
LGAHEVALARGKLSLFAARRSRGGGAEVAPEPLRAIPLLRDLERAAQALGGGRAEPFVTD
CCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
AGEGPLLAGRLRLDGGGELLVLAGEHYPALPPVALAAGEREASGQLALSWPLALAEDERL
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHEECCCCCCCCCEEEECCEEECCCHHH
AAALAAVLEGRGPRRRAYGPPGGPALTRDPGRARLAGWTARLADGGPEAREAARRGGLRA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCH
RSAARLPEALRERAVLVAGCGSVGSYLAELLARAGVGRLALLDPEVVEPANLSRTVFAAE
HHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEHH
DVGRSKPEALARRLLAVEPSIALALEPRAVDGLPPAALDARVREADLVLAATDDPAAQRA
HCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHEEECCEEEEECCCHHHHHH
LDRFAYARGRPALFVGLYAGAQGGEVILTVPGRTACYLCATRSRHGLERAGGRVARELDY
HHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCHHHHCCHHHHHHCC
GTGRLRGEVALAADVQHVASAAAKLALALLAPEGDAGPGPFAEQVIASGATYLTLSTVPG
CCCCCCCCEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCC
YWFYPQVFGDTPGQGAYQSVWLTPARAPDCPVCGPPEARCDPLEVPRRTPDAAAIARALR
CEECHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]