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The map label for this gene is pgaB [H]

Identifier: 220918215

GI number: 220918215

Start: 3441256

End: 3443238

Strand: Reverse

Name: pgaB [H]

Synonym: A2cp1_3118

Alternate gene names: 220918215

Gene position: 3443238-3441256 (Counterclockwise)

Preceding gene: 220918218

Following gene: 220918214

Centisome position: 68.46

GC content: 71.81

Gene sequence:

>1983_bases
ATGACCCTGCGACCCGTGTCACTCCCCGCCGCCGCGGCGCTGCTGCTCGCCGCCGCCGCGCTGCCCGCCTCGCGGACCCT
CGCGGCGACCGAGGAGGAGCTGACCATCCTCAGCTATCACGAGGTGGCGGATCGCGCGGAGGCGCTGGTCCCGCAGTACG
CCGTCTCGCCGACCGCCTTCCTGCGGCAGATGGACTGGCTCCGCAACCACGGCTACCGCTTCGTCTCGGTGGACGACCTG
CTGGCGGCGCGCGAGGGGCGCCGGCCGCTGCCGCCGAAGGCGGTGCTCGTCACCTTCGACGACGCGTACCAGTCGGTCTA
CCGGAACGCCTGGCCGGTCCTGAAGATGTTCCGCATCCCGGCGGTCATCAACGTGGTGGCCGGGTGGCTCGAGGAGAAGG
GGACCGTCGACTTCGACGGCAAGAAGCTGCCGCGGGAGCGGCTCCTCTCCTGGAGCGAGCTGCGCGAGCTCGCCGCCAGC
GGCCTGGTCGAGGTGGGCAGCCACAGCTTCGACCTGCACAAGGGCATCCTGGGCAATCCGCAGGGCAACCTGGAGCCGGC
CGGGACCACGCGACGCTACGACGCGGGCGCGCGGCGCTACGAGGACGAGGCCACCTATCGCCGGCGCGTCCAGGCGGACC
TGGAGCGCAGCGTGGCGCTCATCCGGCGCAACACCGGCCGGGCGCCGCGCGTCGTCGCCTGGCCCTACGGGCGCTACAAC
GCGACCACGCGGGAGACCGCCGAGCGGCTCGGGATGCGCGTCGGCCTGACGCTGGACGACGGCGCGAACACCCGCGACAC
GCCGCTGTGGGGCCTGCGCCGCATCCTGGTCGAGGGCGGGATGGCGCTCTGGGACGTCGAGCGCGAGATCGCCATCCGCA
ACGCGGACCTCTCCGACAACGACCGCCCGCAGAAGGTCATGCACGTGGACCTGGACTACGTGTACGACCCGGATCCGGCG
CAGCAGGAGCGCAACCTCGGTCACCTGCTCGACCGCATCGCCGCCATGGGCGTGAACGCGGTCTACCTGCAGGCGTTCTC
GGACCCGGACGGCAACGGCTCGGCGGACGCGGTGTACTTCCCGTCCCGCCGCATGCCGATGCGCGCCGACCTGTTCAACC
GGGTGGCCTGGCAGATCCGGACGCGCACGCCGGTGAAGCGGCTGTACGCGTGGATGCCGCTGCTGGCGTGGGAGCTGCCG
AAGGGCGACCCGGCCGCGCAGGACCAGGTGGTGACGCTGGCCGGCGACCGCACCGACCGCGTGAACATGGGCTACCACCG
GCTCTCGCCGTTCTCGGCGCGGGCGCGCGAGGCGATCCGCGAGCTGTACCAGGACCTGGCGCGCTCGGCCCCGTTCGACG
GGCTGCTCTTCCACGACGACGCCACGCTCTCCGACTACGAGGACGCCAGCCCCGCGGCGCTGGCGCAGTACCGGGCCTGG
AGCCTGCCCGGGAGCATCGAGGAGATCCGCCGGAGCGACGACCTGCTGGGCCGGTGGACCATCCTCAAGATCAACGCCAT
CGACGACCTCGCCATGGAGGCCGCCGCGGCGGTGCGCGCGCAGCAGCCCGCGCTGGTGGTGGCGCGCAACCTCTACGCGC
GGGTGGCGCTCAACCCCAGGGCGGAGGTCTGGTACTCGCAGGCGCTCGACAACTCCATCGCCCGCTACGACTTCACCGCC
ATCATGGCCATGCCGTACATGGAGAAGGCCGCCGATCACGCCGCCTTCTTCCACGAGCTGGTGCGCGCGGTCGAGTACCG
CGACGCGATGAAGAAGGTCGTGTTCGAGCTGCAGGCGGTGGACTGGCGCGACGGCAACAAGCCCATCCCCAGCCGCGAGC
TCGCCGAGACCATCCGCTCGCTCTACGCGATGGGCGTCATGCACGTCGGCTACTACCCCGACGCGCTGTTCGCCGGGCAG
CCCGATCCGCGGGTCCTGAAGCCGGTGCTCGACTCGAAGCCGAACGCTCCCGCGGTTCGCTGA

Upstream 100 bases:

>100_bases
ACCTGCTGATCATGCCGGCCCGCGGGTGGCCTCCTCCCGCCAAGGGGCGAGCGCCGTACGCCGCCCGGAATTCCTCGTCC
CTGCCGACGTTCCCGACCCG

Downstream 100 bases:

>100_bases
GGCCGCCGCGTGACCGCCACCCTCGACAGCGCGTGGCAAGGCCTGCTGGCCTTCGTGTTCTATTACCCGCTGTTCATGTC
CTTCCTGTGGATGACCGGCG

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTLRPVSLPAAAALLLAAAALPASRTLAATEEELTILSYHEVADRAEALVPQYAVSPTAFLRQMDWLRNHGYRFVSVDDL
LAAREGRRPLPPKAVLVTFDDAYQSVYRNAWPVLKMFRIPAVINVVAGWLEEKGTVDFDGKKLPRERLLSWSELRELAAS
GLVEVGSHSFDLHKGILGNPQGNLEPAGTTRRYDAGARRYEDEATYRRRVQADLERSVALIRRNTGRAPRVVAWPYGRYN
ATTRETAERLGMRVGLTLDDGANTRDTPLWGLRRILVEGGMALWDVEREIAIRNADLSDNDRPQKVMHVDLDYVYDPDPA
QQERNLGHLLDRIAAMGVNAVYLQAFSDPDGNGSADAVYFPSRRMPMRADLFNRVAWQIRTRTPVKRLYAWMPLLAWELP
KGDPAAQDQVVTLAGDRTDRVNMGYHRLSPFSARAREAIRELYQDLARSAPFDGLLFHDDATLSDYEDASPAALAQYRAW
SLPGSIEEIRRSDDLLGRWTILKINAIDDLAMEAAAAVRAQQPALVVARNLYARVALNPRAEVWYSQALDNSIARYDFTA
IMAMPYMEKAADHAAFFHELVRAVEYRDAMKKVVFELQAVDWRDGNKPIPSRELAETIRSLYAMGVMHVGYYPDALFAGQ
PDPRVLKPVLDSKPNAPAVR

Sequences:

>Translated_660_residues
MTLRPVSLPAAAALLLAAAALPASRTLAATEEELTILSYHEVADRAEALVPQYAVSPTAFLRQMDWLRNHGYRFVSVDDL
LAAREGRRPLPPKAVLVTFDDAYQSVYRNAWPVLKMFRIPAVINVVAGWLEEKGTVDFDGKKLPRERLLSWSELRELAAS
GLVEVGSHSFDLHKGILGNPQGNLEPAGTTRRYDAGARRYEDEATYRRRVQADLERSVALIRRNTGRAPRVVAWPYGRYN
ATTRETAERLGMRVGLTLDDGANTRDTPLWGLRRILVEGGMALWDVEREIAIRNADLSDNDRPQKVMHVDLDYVYDPDPA
QQERNLGHLLDRIAAMGVNAVYLQAFSDPDGNGSADAVYFPSRRMPMRADLFNRVAWQIRTRTPVKRLYAWMPLLAWELP
KGDPAAQDQVVTLAGDRTDRVNMGYHRLSPFSARAREAIRELYQDLARSAPFDGLLFHDDATLSDYEDASPAALAQYRAW
SLPGSIEEIRRSDDLLGRWTILKINAIDDLAMEAAAAVRAQQPALVVARNLYARVALNPRAEVWYSQALDNSIARYDFTA
IMAMPYMEKAADHAAFFHELVRAVEYRDAMKKVVFELQAVDWRDGNKPIPSRELAETIRSLYAMGVMHVGYYPDALFAGQ
PDPRVLKPVLDSKPNAPAVR
>Mature_659_residues
TLRPVSLPAAAALLLAAAALPASRTLAATEEELTILSYHEVADRAEALVPQYAVSPTAFLRQMDWLRNHGYRFVSVDDLL
AAREGRRPLPPKAVLVTFDDAYQSVYRNAWPVLKMFRIPAVINVVAGWLEEKGTVDFDGKKLPRERLLSWSELRELAASG
LVEVGSHSFDLHKGILGNPQGNLEPAGTTRRYDAGARRYEDEATYRRRVQADLERSVALIRRNTGRAPRVVAWPYGRYNA
TTRETAERLGMRVGLTLDDGANTRDTPLWGLRRILVEGGMALWDVEREIAIRNADLSDNDRPQKVMHVDLDYVYDPDPAQ
QERNLGHLLDRIAAMGVNAVYLQAFSDPDGNGSADAVYFPSRRMPMRADLFNRVAWQIRTRTPVKRLYAWMPLLAWELPK
GDPAAQDQVVTLAGDRTDRVNMGYHRLSPFSARAREAIRELYQDLARSAPFDGLLFHDDATLSDYEDASPAALAQYRAWS
LPGSIEEIRRSDDLLGRWTILKINAIDDLAMEAAAAVRAQQPALVVARNLYARVALNPRAEVWYSQALDNSIARYDFTAI
MAMPYMEKAADHAAFFHELVRAVEYRDAMKKVVFELQAVDWRDGNKPIPSRELAETIRSLYAMGVMHVGYYPDALFAGQP
DPRVLKPVLDSKPNAPAVR

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=626, Percent_Identity=38.4984025559105, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1786322, Length=257, Percent_Identity=28.0155642023346, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 74163; Mature: 74032

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRPVSLPAAAALLLAAAALPASRTLAATEEELTILSYHEVADRAEALVPQYAVSPTAF
CCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCEEEEHHHHHHHHHHHHCCHHHCCHHHH
LRQMDWLRNHGYRFVSVDDLLAAREGRRPLPPKAVLVTFDDAYQSVYRNAWPVLKMFRIP
HHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
AVINVVAGWLEEKGTVDFDGKKLPRERLLSWSELRELAASGLVEVGSHSFDLHKGILGNP
HHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHCCCCCHHHCCCCCCC
QGNLEPAGTTRRYDAGARRYEDEATYRRRVQADLERSVALIRRNTGRAPRVVAWPYGRYN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
ATTRETAERLGMRVGLTLDDGANTRDTPLWGLRRILVEGGMALWDVEREIAIRNADLSDN
CHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHEEECCCCCCC
DRPQKVMHVDLDYVYDPDPAQQERNLGHLLDRIAAMGVNAVYLQAFSDPDGNGSADAVYF
CCCCEEEEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEC
PSRRMPMRADLFNRVAWQIRTRTPVKRLYAWMPLLAWELPKGDPAAQDQVVTLAGDRTDR
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
VNMGYHRLSPFSARAREAIRELYQDLARSAPFDGLLFHDDATLSDYEDASPAALAQYRAW
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHC
SLPGSIEEIRRSDDLLGRWTILKINAIDDLAMEAAAAVRAQQPALVVARNLYARVALNPR
CCCCHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCH
AEVWYSQALDNSIARYDFTAIMAMPYMEKAADHAAFFHELVRAVEYRDAMKKVVFELQAV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DWRDGNKPIPSRELAETIRSLYAMGVMHVGYYPDALFAGQPDPRVLKPVLDSKPNAPAVR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TLRPVSLPAAAALLLAAAALPASRTLAATEEELTILSYHEVADRAEALVPQYAVSPTAF
CCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCEEEEHHHHHHHHHHHHCCHHHCCHHHH
LRQMDWLRNHGYRFVSVDDLLAAREGRRPLPPKAVLVTFDDAYQSVYRNAWPVLKMFRIP
HHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
AVINVVAGWLEEKGTVDFDGKKLPRERLLSWSELRELAASGLVEVGSHSFDLHKGILGNP
HHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCHHHHCCCCCHHHCCCCCCC
QGNLEPAGTTRRYDAGARRYEDEATYRRRVQADLERSVALIRRNTGRAPRVVAWPYGRYN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
ATTRETAERLGMRVGLTLDDGANTRDTPLWGLRRILVEGGMALWDVEREIAIRNADLSDN
CHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHEEECCCCCCC
DRPQKVMHVDLDYVYDPDPAQQERNLGHLLDRIAAMGVNAVYLQAFSDPDGNGSADAVYF
CCCCEEEEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEC
PSRRMPMRADLFNRVAWQIRTRTPVKRLYAWMPLLAWELPKGDPAAQDQVVTLAGDRTDR
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
VNMGYHRLSPFSARAREAIRELYQDLARSAPFDGLLFHDDATLSDYEDASPAALAQYRAW
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHC
SLPGSIEEIRRSDDLLGRWTILKINAIDDLAMEAAAAVRAQQPALVVARNLYARVALNPR
CCCCHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCH
AEVWYSQALDNSIARYDFTAIMAMPYMEKAADHAAFFHELVRAVEYRDAMKKVVFELQAV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DWRDGNKPIPSRELAETIRSLYAMGVMHVGYYPDALFAGQPDPRVLKPVLDSKPNAPAVR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]