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The map label for this gene is lys20 [H]

Identifier: 220917611

GI number: 220917611

Start: 2777196

End: 2778326

Strand: Direct

Name: lys20 [H]

Synonym: A2cp1_2511

Alternate gene names: 220917611

Gene position: 2777196-2778326 (Clockwise)

Preceding gene: 220917610

Following gene: 220917612

Centisome position: 55.22

GC content: 71.09

Gene sequence:

>1131_bases
ATGACCAAGTCCACCTGGAAGCTCATCGACTCGACGCTCCGCGAGGGCGAGCAGTTCGCCCACGGGACGTTCCGCACCGA
GGACAAGCTCGAGATCGCGCGCGCGCTCGACACGTTCGGGGTCGAGTACGTCGAGGTGACCAGCCCGGCCGCCTCGCCGC
AGTCGCAGCGGGACGCGGTGCAGATCGTGAAGCTCGGGCTCGGCGCGCGGGTCATCACCCACTCGCGCTGCGTCCTCGAC
GACGTGCGCGCCGCCATCGACACCGGCGTCCGCGGCATCGGCCTGCTCTTCGCGACCAGCCGCATCCTCCGCGAGTCCTC
GCACGGCAAGTCCATCCAGCAGATCATCGACGCCATGGGGCCGCCCATCGAGCTGGCGCTCAAGGCCGGCCTCGAGACGC
GCTTCTCCGCCGAGGACGCGTTCCGCAGCGAGGTGACGGACCTGGTGGCGGTCTACCAGGCCGCCGAGCGGCTCGGCGTC
CACCGCGTCGGCATCGCCGACACGGTCGGCATCGCCACGCCGCGCCAGGTGTTCGCGCTGGTGCGCGAGATCCGGCGCGC
GGTCCGCTGCGACATCGGGTTCCACGGCCACAACGACACCGGGTGCTGCATCGCGAACGCCTACGAGGCCCTGGCCGCCG
GCGCCACCCACGTGGACGTGGCGGTGCTGGGGATCGGCGAGCGCGTCGGCATCACCCCGCTCGGCGGCATCATCGCGCGC
ATGTTCTCCATCGAGCCGCAGTCGGTGGCGGAGCGCTACCGGCTCGGCCAGCTCCGCGAGCTGGAGCGGCTGGTGGCCCG
GGTCACCGGCATCGAGGTCCCGTTCAACAACTACGTGACCGGCGAGACCGCGTACTCGCACAAGGCCGGGATGCACCTGA
AGGCCATGATGGCGAACCCCGGCTCCTACGAGATCATCCCGCCGGAGGCGTTCGGCCTCACCCGGCGCCTGATCCTGGGC
AGCCGCCTCACCGGCCGGCACGCCATCGCCTACCGGGCGCGCGAGATGGGCATCACGTTCGGCGAGACCGAGCTGAAGGC
GCTCACCAAGCGCATCAAGGAGCTGGCGGACGCCGGCGAGCTGTCGGAGGAGCAGATCGACCGGGTCCTGCGCGACTGGG
TGACGGCTTGA

Upstream 100 bases:

>100_bases
TTCCAGCCCGAGTACCTGCGCCGCTTCGGCTTCCGGACCGATCCCACCTCGGGCGGCCTGGTCCGCGAGATCGAGCCCGA
CGACTTCCCGCTCTAGCGCC

Downstream 100 bases:

>100_bases
CGGGGCTGCGCCCCGCCGCACGGAGCGGAGCTCCGCGGGGCCCCACCCTGCTCGCGTGTCCCGTGCGCCGGCGCGAGCGG
CTGCCGCCGCTTCCGCGCCG

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKSTWKLIDSTLREGEQFAHGTFRTEDKLEIARALDTFGVEYVEVTSPAASPQSQRDAVQIVKLGLGARVITHSRCVLD
DVRAAIDTGVRGIGLLFATSRILRESSHGKSIQQIIDAMGPPIELALKAGLETRFSAEDAFRSEVTDLVAVYQAAERLGV
HRVGIADTVGIATPRQVFALVREIRRAVRCDIGFHGHNDTGCCIANAYEALAAGATHVDVAVLGIGERVGITPLGGIIAR
MFSIEPQSVAERYRLGQLRELERLVARVTGIEVPFNNYVTGETAYSHKAGMHLKAMMANPGSYEIIPPEAFGLTRRLILG
SRLTGRHAIAYRAREMGITFGETELKALTKRIKELADAGELSEEQIDRVLRDWVTA

Sequences:

>Translated_376_residues
MTKSTWKLIDSTLREGEQFAHGTFRTEDKLEIARALDTFGVEYVEVTSPAASPQSQRDAVQIVKLGLGARVITHSRCVLD
DVRAAIDTGVRGIGLLFATSRILRESSHGKSIQQIIDAMGPPIELALKAGLETRFSAEDAFRSEVTDLVAVYQAAERLGV
HRVGIADTVGIATPRQVFALVREIRRAVRCDIGFHGHNDTGCCIANAYEALAAGATHVDVAVLGIGERVGITPLGGIIAR
MFSIEPQSVAERYRLGQLRELERLVARVTGIEVPFNNYVTGETAYSHKAGMHLKAMMANPGSYEIIPPEAFGLTRRLILG
SRLTGRHAIAYRAREMGITFGETELKALTKRIKELADAGELSEEQIDRVLRDWVTA
>Mature_375_residues
TKSTWKLIDSTLREGEQFAHGTFRTEDKLEIARALDTFGVEYVEVTSPAASPQSQRDAVQIVKLGLGARVITHSRCVLDD
VRAAIDTGVRGIGLLFATSRILRESSHGKSIQQIIDAMGPPIELALKAGLETRFSAEDAFRSEVTDLVAVYQAAERLGVH
RVGIADTVGIATPRQVFALVREIRRAVRCDIGFHGHNDTGCCIANAYEALAAGATHVDVAVLGIGERVGITPLGGIIARM
FSIEPQSVAERYRLGQLRELERLVARVTGIEVPFNNYVTGETAYSHKAGMHLKAMMANPGSYEIIPPEAFGLTRRLILGS
RLTGRHAIAYRAREMGITFGETELKALTKRIKELADAGELSEEQIDRVLRDWVTA

Specific function: Catalyzes The Condensation Of The Acetyl Group Of Acetyl-CoA With 3-Methyl-2-Oxobutanoate (2-Oxoisovalerate) To Form 3-Carboxy-3-Hydroxy-4-Methylpentanoate (2-Isopropylmalate). [C]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=364, Percent_Identity=30.7692307692308, Blast_Score=150, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320071, Length=369, Percent_Identity=50.1355013550135, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=49.4594594594595, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011872
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 41074; Mature: 40942

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSTWKLIDSTLREGEQFAHGTFRTEDKLEIARALDTFGVEYVEVTSPAASPQSQRDAV
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHH
QIVKLGLGARVITHSRCVLDDVRAAIDTGVRGIGLLFATSRILRESSHGKSIQQIIDAMG
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PPIELALKAGLETRFSAEDAFRSEVTDLVAVYQAAERLGVHRVGIADTVGIATPRQVFAL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHH
VREIRRAVRCDIGFHGHNDTGCCIANAYEALAAGATHVDVAVLGIGERVGITPLGGIIAR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHH
MFSIEPQSVAERYRLGQLRELERLVARVTGIEVPFNNYVTGETAYSHKAGMHLKAMMANP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCEEEEEECCC
GSYEIIPPEAFGLTRRLILGSRLTGRHAIAYRAREMGITFGETELKALTKRIKELADAGE
CCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
LSEEQIDRVLRDWVTA
CCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKSTWKLIDSTLREGEQFAHGTFRTEDKLEIARALDTFGVEYVEVTSPAASPQSQRDAV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHH
QIVKLGLGARVITHSRCVLDDVRAAIDTGVRGIGLLFATSRILRESSHGKSIQQIIDAMG
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PPIELALKAGLETRFSAEDAFRSEVTDLVAVYQAAERLGVHRVGIADTVGIATPRQVFAL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHH
VREIRRAVRCDIGFHGHNDTGCCIANAYEALAAGATHVDVAVLGIGERVGITPLGGIIAR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHH
MFSIEPQSVAERYRLGQLRELERLVARVTGIEVPFNNYVTGETAYSHKAGMHLKAMMANP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCEEEEEECCC
GSYEIIPPEAFGLTRRLILGSRLTGRHAIAYRAREMGITFGETELKALTKRIKELADAGE
CCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
LSEEQIDRVLRDWVTA
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9868782 [H]