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The map label for this gene is eno

Identifier: 220917405

GI number: 220917405

Start: 2555290

End: 2556579

Strand: Reverse

Name: eno

Synonym: A2cp1_2305

Alternate gene names: 220917405

Gene position: 2556579-2555290 (Counterclockwise)

Preceding gene: 220917406

Following gene: 220917404

Centisome position: 50.83

GC content: 71.16

Gene sequence:

>1290_bases
ATGACCGAGATCATCAACGTGACCGCGCGGGAGATCCTGGATTCCCGCGGCAACCCCACCGTCGAGGTCGAGGTGGCGGT
CGGGACCGGCGACGTGGGGCGCGCGGCGGTGCCCTCCGGCGCGTCCACCGGCGAGCACGAGGCGCTCGAGCTCCGCGACG
GCGACAAGGGCCGGTACCTGGGCAAGGGCGTCCGGAAGGCCGTCGCGAACGTGATCGACGAGATCGCGCCCGCGGTGGTC
GGCCTCGACGCCTCCGACCAGGCCTCGCTCGACGCGCGCATGATCGCGCTCGACGGCACCCCCACCAAGTCGAAGCTGGG
CGCGAACGCCATCCTGGGCGTGTCGCTGGCCGCGGCCAAGGCCGCCGCCACGGCGCACGGCCTCCCGCTCTACCGCTACG
TCGGCGGGGCCGGGGCGCGCACGCTCCCGGTGCCGCTCATGAACATCCTGAACGGCGGCGCGCACGCCGACTCCAACGTG
GACATCCAGGAGTTCATGGTGGTGCCGCTCGGCCTGCCCACCTTCGCCGAGGCGCTCCGCTGCGGCGCCGAGATCTTCCA
CGCGCTGAAGAAGGTGCTGAAGGGGAAGGGCGCGGCGACGGGCGTGGGCGACGAGGGCGGCTACGCGCCGAGCCTCGCCT
CGAACGAGGAGGCGCTCGCGGTGATCATGGAGGCCATCGGCCAGGCCGGCTACGAGCCGGGCAAGCAGGTGGCGCTCGCG
CTCGACTGCGCGGCCAGCGAGTTCTACGACAAGAAGGCCGGCAAGTACGAGCTGGAGGGCGAGGGCAAGCGCTTCGACGG
CAAGGGGCTGGTGGAGTACTACGCCCAGCTCGCCGCCAAGTACCCCATCGTCTCGATCGAGGACGGCTGCGACGAGGACG
ACTGGGCGACCTGGAAGCTCCTGACCGAGCGCCTGGGCGGGAAGCTGCAGCTCGTCGGCGACGATCTGTTCGTCACCAAC
GTGACCCGCCTCGCGCGCGGCATCGAGCAGGGCGTGACGAACTCGATCCTCGTGAAGGTGAACCAGATCGGCTCGCTCAC
CGAGACGCTGGAGGCGGTCCGCATGGCGCACCGCGCCGGCTACACCACCGTGATGAGCCACCGCTCCGGCGAGACCGAGG
ACACCACCATCGCCGACCTGGCGGTCGCCTGCGACTGCGGGCAGATCAAGACCGGCTCGGCGTCGCGCACCGACCGCATC
GCCAAGTACAACCAGCTGCTCCGCATCGAGGAGGAGCTGGGTGGATCCGGCCGGTACGCCGGCCGCAGCGCGTTCAAGGC
GCTCCGCTGA

Upstream 100 bases:

>100_bases
AACCGATTGACCCTTCGACTGCGCGCGGGGCACATTCGCGGGCCTGCGAGCGGATCCCTTGGGCGCGGCGACCGCGCCGG
GCGCTCGGGGAGACCAGCCG

Downstream 100 bases:

>100_bases
GCCCCCCGGCGAGCGGGCCCCGGACCTCGCGCCGGGGCCCGCCGCGCCGACCCCGAGCAGCCCCGCCGCACGCGCCCCGG
GTGCGGGCGCAACCCCGCGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTEIINVTAREILDSRGNPTVEVEVAVGTGDVGRAAVPSGASTGEHEALELRDGDKGRYLGKGVRKAVANVIDEIAPAVV
GLDASDQASLDARMIALDGTPTKSKLGANAILGVSLAAAKAAATAHGLPLYRYVGGAGARTLPVPLMNILNGGAHADSNV
DIQEFMVVPLGLPTFAEALRCGAEIFHALKKVLKGKGAATGVGDEGGYAPSLASNEEALAVIMEAIGQAGYEPGKQVALA
LDCAASEFYDKKAGKYELEGEGKRFDGKGLVEYYAQLAAKYPIVSIEDGCDEDDWATWKLLTERLGGKLQLVGDDLFVTN
VTRLARGIEQGVTNSILVKVNQIGSLTETLEAVRMAHRAGYTTVMSHRSGETEDTTIADLAVACDCGQIKTGSASRTDRI
AKYNQLLRIEEELGGSGRYAGRSAFKALR

Sequences:

>Translated_429_residues
MTEIINVTAREILDSRGNPTVEVEVAVGTGDVGRAAVPSGASTGEHEALELRDGDKGRYLGKGVRKAVANVIDEIAPAVV
GLDASDQASLDARMIALDGTPTKSKLGANAILGVSLAAAKAAATAHGLPLYRYVGGAGARTLPVPLMNILNGGAHADSNV
DIQEFMVVPLGLPTFAEALRCGAEIFHALKKVLKGKGAATGVGDEGGYAPSLASNEEALAVIMEAIGQAGYEPGKQVALA
LDCAASEFYDKKAGKYELEGEGKRFDGKGLVEYYAQLAAKYPIVSIEDGCDEDDWATWKLLTERLGGKLQLVGDDLFVTN
VTRLARGIEQGVTNSILVKVNQIGSLTETLEAVRMAHRAGYTTVMSHRSGETEDTTIADLAVACDCGQIKTGSASRTDRI
AKYNQLLRIEEELGGSGRYAGRSAFKALR
>Mature_428_residues
TEIINVTAREILDSRGNPTVEVEVAVGTGDVGRAAVPSGASTGEHEALELRDGDKGRYLGKGVRKAVANVIDEIAPAVVG
LDASDQASLDARMIALDGTPTKSKLGANAILGVSLAAAKAAATAHGLPLYRYVGGAGARTLPVPLMNILNGGAHADSNVD
IQEFMVVPLGLPTFAEALRCGAEIFHALKKVLKGKGAATGVGDEGGYAPSLASNEEALAVIMEAIGQAGYEPGKQVALAL
DCAASEFYDKKAGKYELEGEGKRFDGKGLVEYYAQLAAKYPIVSIEDGCDEDDWATWKLLTERLGGKLQLVGDDLFVTNV
TRLARGIEQGVTNSILVKVNQIGSLTETLEAVRMAHRAGYTTVMSHRSGETEDTTIADLAVACDCGQIKTGSASRTDRIA
KYNQLLRIEEELGGSGRYAGRSAFKALR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=51.3888888888889, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=53.8461538461538, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=53.8461538461538, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=52.5462962962963, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=49.4145199063232, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=344, Percent_Identity=26.7441860465116, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI169201757, Length=344, Percent_Identity=26.7441860465116, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI239744207, Length=344, Percent_Identity=26.7441860465116, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=63.5730858468677, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.183908045977, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.183908045977, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=196, Percent_Identity=48.469387755102, Blast_Score=184, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=435, Percent_Identity=51.7241379310345, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=50.9216589861751, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=50.9216589861751, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=50.6912442396313, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=51.0344827586207, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=51.1627906976744, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=51.1627906976744, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=51.1627906976744, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=51.1627906976744, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=51.1627906976744, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=51.1627906976744, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ANAD2 (B8JAC4)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492709.1
- ProteinModelPortal:   B8JAC4
- SMR:   B8JAC4
- GeneID:   7299271
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2305
- HOGENOM:   HBG726599
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45008; Mature: 44877

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 339-339 BINDING 155-155 BINDING 164-164 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIINVTAREILDSRGNPTVEVEVAVGTGDVGRAAVPSGASTGEHEALELRDGDKGRYL
CCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
GKGVRKAVANVIDEIAPAVVGLDASDQASLDARMIALDGTPTKSKLGANAILGVSLAAAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAATAHGLPLYRYVGGAGARTLPVPLMNILNGGAHADSNVDIQEFMVVPLGLPTFAEALR
HHHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
CGAEIFHALKKVLKGKGAATGVGDEGGYAPSLASNEEALAVIMEAIGQAGYEPGKQVALA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYDKKAGKYELEGEGKRFDGKGLVEYYAQLAAKYPIVSIEDGCDEDDWATWKL
EEHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LTERLGGKLQLVGDDLFVTNVTRLARGIEQGVTNSILVKVNQIGSLTETLEAVRMAHRAG
HHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHCC
YTTVMSHRSGETEDTTIADLAVACDCGQIKTGSASRTDRIAKYNQLLRIEEELGGSGRYA
CHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GRSAFKALR
CHHHHHHCC
>Mature Secondary Structure 
TEIINVTAREILDSRGNPTVEVEVAVGTGDVGRAAVPSGASTGEHEALELRDGDKGRYL
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
GKGVRKAVANVIDEIAPAVVGLDASDQASLDARMIALDGTPTKSKLGANAILGVSLAAAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAATAHGLPLYRYVGGAGARTLPVPLMNILNGGAHADSNVDIQEFMVVPLGLPTFAEALR
HHHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
CGAEIFHALKKVLKGKGAATGVGDEGGYAPSLASNEEALAVIMEAIGQAGYEPGKQVALA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAASEFYDKKAGKYELEGEGKRFDGKGLVEYYAQLAAKYPIVSIEDGCDEDDWATWKL
EEHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LTERLGGKLQLVGDDLFVTNVTRLARGIEQGVTNSILVKVNQIGSLTETLEAVRMAHRAG
HHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHCC
YTTVMSHRSGETEDTTIADLAVACDCGQIKTGSASRTDRIAKYNQLLRIEEELGGSGRYA
CHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GRSAFKALR
CHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA