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The map label for this gene is mptN [H]

Identifier: 220917354

GI number: 220917354

Start: 2505558

End: 2506502

Strand: Direct

Name: mptN [H]

Synonym: A2cp1_2254

Alternate gene names: 220917354

Gene position: 2505558-2506502 (Clockwise)

Preceding gene: 220917353

Following gene: 220917355

Centisome position: 49.82

GC content: 75.77

Gene sequence:

>945_bases
ATGGAACGCTTCATCCGGATCGGGGTGGTGACCGCCTGGCCGGAGGAGGACTGGCACAGCAAGCGCCTGCTCGCTGCGTG
TGCCCGGCGGGGCGAGGCGGTGGTGATCGATCCGTCGGCGATGGCGGCCGGCGTGGGGGCGGAGGGCGTGGGCGTCTCGC
TCGGCACCATGCGGATGGAGGAGGTCGATGCGTTCGTGCTCGCGCGCGGCCTCGGCCGGGAGGGCGACCCCGACGCGCAG
TTCGAGATCTACCGCGCGATGGAGGGAGCCGGCGCGGTGGTGGTGAACCGGATCGAGCCGCTGCTCGCGGCCCAGGACAA
GTTCCGCAGCTCGTGGCTCCTGGTCCGCGCCGGCGTGCCGACCCCGCCGGCGGCGGTGGCCCAGGCGCCGGTCGGCGCCG
AGCACGCGCTGGAGCGGCTCGGCGAGTCCGTCGTGAAGCCACTGGCGGGCTCGCTCGGCGAGGGCGTGGAGCGCGTCCGC
CCGGATCGGGCGGGGCGAGACCGCGTCCGGGAGCGGGCGGGAAGGGACGGCGCGGTGTACCTGCAGGCGTACGTCCCGCA
CCCGGGGCGAGACCTGCGCGTGTTCGTGGTCGGGGGCACGACTCGGGCCGCGATGGTGCGGCACGCGCCGCCGGGCGAGT
GGCGCACCAACGTGGGCGGCGGCGGGCGCGTGGAGGCGGTGGAGTGCCCCGCCGCGGTGCGCGCGGTGGCGGAGGCGGCG
GCGGCGGCGCTCGAGCTGGACTACGCCGGCGTGGACCTCGTGCTGGGCGACGAGGGCCCGACGGTGATCGAGGTGAACGG
GAACCCGAGCTGGCAGGGGATCCTCGAGGCCACCGGCCTGGACATGGCGGAGGCCATCGCCGAGCACGTGCTCGCCCGGG
CCCTGCGCCGGCGGAAGACCAGCGACCACATTGTGCGTGAACGGACGGGGGCGACACATGGCTGA

Upstream 100 bases:

>100_bases
CTCCGAGAAAGGTTCGGGATGGCGCGCCCTTGTCCAGCCAGCCGGGCATGGTCACCATGTTGCGCGGCGGTCGGGGTGGG
GGAAGGGAAGCAAGCCTCGC

Downstream 100 bases:

>100_bases
AGAGAAGAAGGGCGGCAGCAAGGGGGCGATGACGGTCTCGGAGGCCGGTCGCAAGGGCGGGGTGCGGGTCCGTGAGACGC
GCGGCCGCAAGTTCTACGAG

Product: RimK family alpha-L-glutamate ligase

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MERFIRIGVVTAWPEEDWHSKRLLAACARRGEAVVIDPSAMAAGVGAEGVGVSLGTMRMEEVDAFVLARGLGREGDPDAQ
FEIYRAMEGAGAVVVNRIEPLLAAQDKFRSSWLLVRAGVPTPPAAVAQAPVGAEHALERLGESVVKPLAGSLGEGVERVR
PDRAGRDRVRERAGRDGAVYLQAYVPHPGRDLRVFVVGGTTRAAMVRHAPPGEWRTNVGGGGRVEAVECPAAVRAVAEAA
AAALELDYAGVDLVLGDEGPTVIEVNGNPSWQGILEATGLDMAEAIAEHVLARALRRRKTSDHIVRERTGATHG

Sequences:

>Translated_314_residues
MERFIRIGVVTAWPEEDWHSKRLLAACARRGEAVVIDPSAMAAGVGAEGVGVSLGTMRMEEVDAFVLARGLGREGDPDAQ
FEIYRAMEGAGAVVVNRIEPLLAAQDKFRSSWLLVRAGVPTPPAAVAQAPVGAEHALERLGESVVKPLAGSLGEGVERVR
PDRAGRDRVRERAGRDGAVYLQAYVPHPGRDLRVFVVGGTTRAAMVRHAPPGEWRTNVGGGGRVEAVECPAAVRAVAEAA
AAALELDYAGVDLVLGDEGPTVIEVNGNPSWQGILEATGLDMAEAIAEHVLARALRRRKTSDHIVRERTGATHG
>Mature_314_residues
MERFIRIGVVTAWPEEDWHSKRLLAACARRGEAVVIDPSAMAAGVGAEGVGVSLGTMRMEEVDAFVLARGLGREGDPDAQ
FEIYRAMEGAGAVVVNRIEPLLAAQDKFRSSWLLVRAGVPTPPAAVAQAPVGAEHALERLGESVVKPLAGSLGEGVERVR
PDRAGRDRVRERAGRDGAVYLQAYVPHPGRDLRVFVVGGTTRAAMVRHAPPGEWRTNVGGGGRVEAVECPAAVRAVAEAA
AAALELDYAGVDLVLGDEGPTVIEVNGNPSWQGILEATGLDMAEAIAEHVLARALRRRKTSDHIVRERTGATHG

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=303, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787076, Length=293, Percent_Identity=27.9863481228669, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33284; Mature: 33284

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFIRIGVVTAWPEEDWHSKRLLAACARRGEAVVIDPSAMAAGVGAEGVGVSLGTMRME
CCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCEEHHHHHHH
EVDAFVLARGLGREGDPDAQFEIYRAMEGAGAVVVNRIEPLLAAQDKFRSSWLLVRAGVP
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCC
TPPAAVAQAPVGAEHALERLGESVVKPLAGSLGEGVERVRPDRAGRDRVRERAGRDGAVY
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
LQAYVPHPGRDLRVFVVGGTTRAAMVRHAPPGEWRTNVGGGGRVEAVECPAAVRAVAEAA
EEEECCCCCCCEEEEEECCCCHHHHEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
AAALELDYAGVDLVLGDEGPTVIEVNGNPSWQGILEATGLDMAEAIAEHVLARALRRRKT
HHHHEEECCCEEEEECCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
SDHIVRERTGATHG
HHHHHHHCCCCCCC
>Mature Secondary Structure
MERFIRIGVVTAWPEEDWHSKRLLAACARRGEAVVIDPSAMAAGVGAEGVGVSLGTMRME
CCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCEEHHHHHHH
EVDAFVLARGLGREGDPDAQFEIYRAMEGAGAVVVNRIEPLLAAQDKFRSSWLLVRAGVP
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCC
TPPAAVAQAPVGAEHALERLGESVVKPLAGSLGEGVERVRPDRAGRDRVRERAGRDGAVY
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
LQAYVPHPGRDLRVFVVGGTTRAAMVRHAPPGEWRTNVGGGGRVEAVECPAAVRAVAEAA
EEEECCCCCCCEEEEEECCCCHHHHEECCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
AAALELDYAGVDLVLGDEGPTVIEVNGNPSWQGILEATGLDMAEAIAEHVLARALRRRKT
HHHHEEECCCEEEEECCCCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCC
SDHIVRERTGATHG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]