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The map label for this gene is greA

Identifier: 220917309

GI number: 220917309

Start: 2451722

End: 2452222

Strand: Direct

Name: greA

Synonym: A2cp1_2209

Alternate gene names: 220917309

Gene position: 2451722-2452222 (Clockwise)

Preceding gene: 220917308

Following gene: 220917310

Centisome position: 48.75

GC content: 68.66

Gene sequence:

>501_bases
ATGCAACGCGTCCCCATGACCAAGGGCGGCCTCGTCCGGCTGAAGGATGAGCTGAGGCGGCTCAAGAGCGTCGAGCGTCC
CAAGATCGTGAAGGAGATCGCGGAGGCGCGCGCGCACGGGGATCTCTCCGAGAACGCCGAGTACCACGCGGCCAAGGAGA
AGCAGAGCCACATCGAGGGCCGGATCGCGCAGGTGGAGCACTGGATCGCCAGCGCCGAGGTCATCGACGTGACCAAGCAC
GCCGGCGACCGCGTGGTGTTCGGCGCGACCGTCTCGCTCGAGGACGCCGAGTCCGGCGACCAGGTGACGTACCGCATCGT
GGGCGAGCTGGAGGCCGACCTGAAGCAGGGCAAGATCTCGGTCACGAGCCCGATCGCGCGCGCGCTCATCGGGCGCAGCG
AGGGCGACACGGTGGTGGTGCGCTCGCCCGGCGGCGAGAAGGAGTACGAGATCCAGTCGGTCGCGTTCGTGGAGGAGGAG
CTGCCGACGGAGTCCGAGTAG

Upstream 100 bases:

>100_bases
GTTGACAGCGCCTCCGAGGCGCTCTAGTGAAGGAAGAGGAACGCGGCCGCTCCGGGCATCCCCGGGGTCGGCCGTCCTCG
TTTTTCGGGAAGGTGAAACG

Downstream 100 bases:

>100_bases
TCGGACCCGGCTGGTAGCCTCGCCGCCGTGGAGCGCAAGCAGGGCGGCCCGGCGGATCCGCGCCAGGAGGGAGACCTCCC
CGGCGCCGCCGCCGCGCGCG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MQRVPMTKGGLVRLKDELRRLKSVERPKIVKEIAEARAHGDLSENAEYHAAKEKQSHIEGRIAQVEHWIASAEVIDVTKH
AGDRVVFGATVSLEDAESGDQVTYRIVGELEADLKQGKISVTSPIARALIGRSEGDTVVVRSPGGEKEYEIQSVAFVEEE
LPTESE

Sequences:

>Translated_166_residues
MQRVPMTKGGLVRLKDELRRLKSVERPKIVKEIAEARAHGDLSENAEYHAAKEKQSHIEGRIAQVEHWIASAEVIDVTKH
AGDRVVFGATVSLEDAESGDQVTYRIVGELEADLKQGKISVTSPIARALIGRSEGDTVVVRSPGGEKEYEIQSVAFVEEE
LPTESE
>Mature_166_residues
MQRVPMTKGGLVRLKDELRRLKSVERPKIVKEIAEARAHGDLSENAEYHAAKEKQSHIEGRIAQVEHWIASAEVIDVTKH
AGDRVVFGATVSLEDAESGDQVTYRIVGELEADLKQGKISVTSPIARALIGRSEGDTVVVRSPGGEKEYEIQSVAFVEEE
LPTESE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=55.6962025316456, Blast_Score=176, Evalue=5e-46,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=32.9113924050633, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_ANASK (B4UCU9)

Other databases:

- EMBL:   CP001131
- RefSeq:   YP_002134475.1
- GeneID:   6787726
- GenomeReviews:   CP001131_GR
- KEGG:   ank:AnaeK_2119
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18272; Mature: 18272

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVPMTKGGLVRLKDELRRLKSVERPKIVKEIAEARAHGDLSENAEYHAAKEKQSHIEG
CCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIAQVEHWIASAEVIDVTKHAGDRVVFGATVSLEDAESGDQVTYRIVGELEADLKQGKIS
HHHHHHHHHHHHHHEEHHHCCCCEEEEEEEEEECCCCCCCEEEEEEEEHHHHHHHCCCEE
VTSPIARALIGRSEGDTVVVRSPGGEKEYEIQSVAFVEEELPTESE
EHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQRVPMTKGGLVRLKDELRRLKSVERPKIVKEIAEARAHGDLSENAEYHAAKEKQSHIEG
CCCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIAQVEHWIASAEVIDVTKHAGDRVVFGATVSLEDAESGDQVTYRIVGELEADLKQGKIS
HHHHHHHHHHHHHHEEHHHCCCCEEEEEEEEEECCCCCCCEEEEEEEEHHHHHHHCCCEE
VTSPIARALIGRSEGDTVVVRSPGGEKEYEIQSVAFVEEELPTESE
EHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA