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The map label for this gene is pcrA [H]

Identifier: 220917224

GI number: 220917224

Start: 2371321

End: 2373501

Strand: Direct

Name: pcrA [H]

Synonym: A2cp1_2122

Alternate gene names: 220917224

Gene position: 2371321-2373501 (Clockwise)

Preceding gene: 220917222

Following gene: 220917225

Centisome position: 47.15

GC content: 72.95

Gene sequence:

>2181_bases
ATGTCCCGGCATTACCAGCTCAAGGCGGCCTCCCCGCCCGCCCGCGCGCTCAACTACCAGGGGCTGCTCAACGACCAGCA
GATCGCCGTGGTCGAGGCGGGCGACGGCCCGATCCTGGTGATCGCCGGCGCGGGCTCCGGGAAGACGCGCACGCTCACCT
GGCGCGTGGCGCGGCTGGTCGCCGATGGCGTCGCGCCGGAGGGCATCCTCCTCCTCACGTTCACCAACAAGGCGGCGCGC
GAGATGCTCCGCCGCGTGGAGGAGGTCTGCCGGGTGGACACCCGCCGCATCTCCGGCGGCACGTTTCACCACGTGGCGCA
CGAGGTGCTGCGCGAGCACGCCGCGGAGCTGGGGTACCAGCGCGGCTACTCGATCCTCGATCGCGAGGACTCGCGCGACG
TGATGACCGCCGCCATCGCGGAGTGCGGGCTCGCGGTGGGCGCGCGCCGCTTCCCGAAGGCGGACGTGCTCATCGACCTC
GTCTCGATGGCGGTGAACACCCAGACGCCGCTCGCCGACGTCCTCGCCGACCGGCGCCCGCAGTTCGTGCCGCTGACCGA
CGACGTGCTGCGGGTGGCCCGCCGCTACGCGGAGCGCAAGCACGCGCTCAACGCGATGGACTTCGACGACCTGCTGCTCA
ACTGGAAGATCCTGCTCGCCGAGCGCGAGCACGTCCGCCGCGCGCTGGCCGGGCGCTACCGGCACGTGCTGGTGGACGAG
TACCAGGACACGAACCGGCTGCAGGGCGACGTGGTGGATCTCATCGCCGCCGAGCACCGGAACCTGTGCGTGGTGGGCGA
CGACGCGCAGAGCATCTACTCGTTCCGCGGGGCGCACTTCGCGAACATCCTCGAGTTCGAGAAGCGCTACCCGGACGCGC
GGCGCTTCGACCTCACCGTCAACTACCGCTCCACCCCGCAGATCCTCTCGCTCGCGAACGCGTCCATCGCCGCGAACGTC
CGCCAGTTCAAGAAGGAGCTGTCGAGCGTCCGGCGCGACGGGACGCTGCCCGCGGTGGTGCCGTGCCGCGACGTGCAGCA
GCAGGCGGCGTTCGTGGCGCAGCGGGTGCTGGAGCTGCGCGACGAGGGCATCCCGCTCCCCGAGATCGCGGTGCTGTACC
GGGCGCACCACCACTCGATGGAGATCCAGTTCGAGCTGGCGCGCCGCGGGATCCCGTTCGTCGTCCGCAGCGGGGTCCGC
TTCTTCGAGGCCGCGCACGTGAAGGACGTGCTCGCCCACCTGCGCTTCGCGCGGAACCCGGGCGACGAGCTGGCGCTGAA
GCGCTGCCTCAAGATCACCCCCGGCGTCGGCACCGCCACCGCCGACGCGGTGTGGAGCGCGTTCTCGCTGCGGCGCAGCC
GCGGGCGCGGCACGCTGGACGAGCTGCTCGCGCCCGACGTCGCCTCGCACGTCCCGGCCAAGGGGCGCGCCGGCTACACC
CGGCTCGCCCAGCTCCTGCGCGCGCTGGGCCGCCCGCCCACCTCCGACCTGCCGGGCGAGGCCATCGAGAAGGTGCTGGA
CGGCGGCTACGAGGAGTACCTGCGGGCCGAGTTCCTGAACGCCGATCAGCGCGTCGAGGACGTCCAGCAGCTCGCGCAGT
ACGCGCGCGGCTACGAGGACACCGAGGCGTTCCTCTCCGAGATCGCGCTGCTCACCGAGATCTCGGCCGAGACCGTGTCG
GAGGGCGGCGAGCCGGACGAGAAGATGATCCTCTCGTCGGTGCACCAGGCGAAGGGCCTCGAGTGGCGGGCGGTCTTCGT
GGTGTGGCTCGCCGACGGTCGCTTCCCGTCGGCGCAGGCGCTGAAGGACACCGACGGCGAGGAGGAGGAGCGGCGGCTGT
TCTACGTCGCCTCGACGCGCGCGAAGGACGAGCTGTACCTGACGTACCCGGTGGTCGCCGCGCCGCGAGACCGCGAGCGG
GTGGTCATGAAGGCCTCGCGCTTCCTCGAGGAGCTGCCCGCCGAGCCGGAGCTGTTCGAGCGCTGGCAGCTCGACGATCC
CGGCCTCCCGGCGGCGCTGGGCGCGGCGCCGCCGCCGGCGGCCGGGCTCCCGCCGCGCGATCCGAGCGTCGTCCCCGCGC
TGTTCGGCGAGCCTGCGCCGAGGCGCGGGCCCGCCGACGTCGTCGCGCCGGTGGAGGCGGACGGCGGGGACCCGGGCGGC
GGCGACGACGTGCCGTTCTAG

Upstream 100 bases:

>100_bases
TTTTCGGCCGCAATTGACGCATCGTGAAGTCGTCGCCCGCGGAAGCGACCGCCGGTTTGACCCGGCCGATATCGCCTGCT
AGGTTGCCTGCCCTGAACGC

Downstream 100 bases:

>100_bases
GGCGGCGGCGAGTCCGGGGCGGGTCGCGCTCCGGGTTCGGGTGTCGCCGGTTCCGTGCTAGGTTTCGGGGCCATGGCAGG
CGCGCGGGAAAAGCCCATCA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSRHYQLKAASPPARALNYQGLLNDQQIAVVEAGDGPILVIAGAGSGKTRTLTWRVARLVADGVAPEGILLLTFTNKAAR
EMLRRVEEVCRVDTRRISGGTFHHVAHEVLREHAAELGYQRGYSILDREDSRDVMTAAIAECGLAVGARRFPKADVLIDL
VSMAVNTQTPLADVLADRRPQFVPLTDDVLRVARRYAERKHALNAMDFDDLLLNWKILLAEREHVRRALAGRYRHVLVDE
YQDTNRLQGDVVDLIAAEHRNLCVVGDDAQSIYSFRGAHFANILEFEKRYPDARRFDLTVNYRSTPQILSLANASIAANV
RQFKKELSSVRRDGTLPAVVPCRDVQQQAAFVAQRVLELRDEGIPLPEIAVLYRAHHHSMEIQFELARRGIPFVVRSGVR
FFEAAHVKDVLAHLRFARNPGDELALKRCLKITPGVGTATADAVWSAFSLRRSRGRGTLDELLAPDVASHVPAKGRAGYT
RLAQLLRALGRPPTSDLPGEAIEKVLDGGYEEYLRAEFLNADQRVEDVQQLAQYARGYEDTEAFLSEIALLTEISAETVS
EGGEPDEKMILSSVHQAKGLEWRAVFVVWLADGRFPSAQALKDTDGEEEERRLFYVASTRAKDELYLTYPVVAAPRDRER
VVMKASRFLEELPAEPELFERWQLDDPGLPAALGAAPPPAAGLPPRDPSVVPALFGEPAPRRGPADVVAPVEADGGDPGG
GDDVPF

Sequences:

>Translated_726_residues
MSRHYQLKAASPPARALNYQGLLNDQQIAVVEAGDGPILVIAGAGSGKTRTLTWRVARLVADGVAPEGILLLTFTNKAAR
EMLRRVEEVCRVDTRRISGGTFHHVAHEVLREHAAELGYQRGYSILDREDSRDVMTAAIAECGLAVGARRFPKADVLIDL
VSMAVNTQTPLADVLADRRPQFVPLTDDVLRVARRYAERKHALNAMDFDDLLLNWKILLAEREHVRRALAGRYRHVLVDE
YQDTNRLQGDVVDLIAAEHRNLCVVGDDAQSIYSFRGAHFANILEFEKRYPDARRFDLTVNYRSTPQILSLANASIAANV
RQFKKELSSVRRDGTLPAVVPCRDVQQQAAFVAQRVLELRDEGIPLPEIAVLYRAHHHSMEIQFELARRGIPFVVRSGVR
FFEAAHVKDVLAHLRFARNPGDELALKRCLKITPGVGTATADAVWSAFSLRRSRGRGTLDELLAPDVASHVPAKGRAGYT
RLAQLLRALGRPPTSDLPGEAIEKVLDGGYEEYLRAEFLNADQRVEDVQQLAQYARGYEDTEAFLSEIALLTEISAETVS
EGGEPDEKMILSSVHQAKGLEWRAVFVVWLADGRFPSAQALKDTDGEEEERRLFYVASTRAKDELYLTYPVVAAPRDRER
VVMKASRFLEELPAEPELFERWQLDDPGLPAALGAAPPPAAGLPPRDPSVVPALFGEPAPRRGPADVVAPVEADGGDPGG
GDDVPF
>Mature_725_residues
SRHYQLKAASPPARALNYQGLLNDQQIAVVEAGDGPILVIAGAGSGKTRTLTWRVARLVADGVAPEGILLLTFTNKAARE
MLRRVEEVCRVDTRRISGGTFHHVAHEVLREHAAELGYQRGYSILDREDSRDVMTAAIAECGLAVGARRFPKADVLIDLV
SMAVNTQTPLADVLADRRPQFVPLTDDVLRVARRYAERKHALNAMDFDDLLLNWKILLAEREHVRRALAGRYRHVLVDEY
QDTNRLQGDVVDLIAAEHRNLCVVGDDAQSIYSFRGAHFANILEFEKRYPDARRFDLTVNYRSTPQILSLANASIAANVR
QFKKELSSVRRDGTLPAVVPCRDVQQQAAFVAQRVLELRDEGIPLPEIAVLYRAHHHSMEIQFELARRGIPFVVRSGVRF
FEAAHVKDVLAHLRFARNPGDELALKRCLKITPGVGTATADAVWSAFSLRRSRGRGTLDELLAPDVASHVPAKGRAGYTR
LAQLLRALGRPPTSDLPGEAIEKVLDGGYEEYLRAEFLNADQRVEDVQQLAQYARGYEDTEAFLSEIALLTEISAETVSE
GGEPDEKMILSSVHQAKGLEWRAVFVVWLADGRFPSAQALKDTDGEEEERRLFYVASTRAKDELYLTYPVVAAPRDRERV
VMKASRFLEELPAEPELFERWQLDDPGLPAALGAAPPPAAGLPPRDPSVVPALFGEPAPRRGPADVVAPVEADGGDPGGG
DDVPF

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=653, Percent_Identity=32.9249617151608, Blast_Score=277, Evalue=2e-75,
Organism=Escherichia coli, GI48994965, Length=665, Percent_Identity=31.2781954887218, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1787196, Length=399, Percent_Identity=28.3208020050125, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=549, Percent_Identity=26.5938069216758, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324477, Length=673, Percent_Identity=22.8826151560178, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80321; Mature: 80190

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRHYQLKAASPPARALNYQGLLNDQQIAVVEAGDGPILVIAGAGSGKTRTLTWRVARLV
CCCCEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHH
ADGVAPEGILLLTFTNKAAREMLRRVEEVCRVDTRRISGGTFHHVAHEVLREHAAELGYQ
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RGYSILDREDSRDVMTAAIAECGLAVGARRFPKADVLIDLVSMAVNTQTPLADVLADRRP
CCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
QFVPLTDDVLRVARRYAERKHALNAMDFDDLLLNWKILLAEREHVRRALAGRYRHVLVDE
CEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
YQDTNRLQGDVVDLIAAEHRNLCVVGDDAQSIYSFRGAHFANILEFEKRYPDARRFDLTV
HCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
NYRSTPQILSLANASIAANVRQFKKELSSVRRDGTLPAVVPCRDVQQQAAFVAQRVLELR
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHH
DEGIPLPEIAVLYRAHHHSMEIQFELARRGIPFVVRSGVRFFEAAHVKDVLAHLRFARNP
HCCCCCHHHHHHHHHHHCCEEEEEHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCC
GDELALKRCLKITPGVGTATADAVWSAFSLRRSRGRGTLDELLAPDVASHVPAKGRAGYT
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCHH
RLAQLLRALGRPPTSDLPGEAIEKVLDGGYEEYLRAEFLNADQRVEDVQQLAQYARGYED
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCH
TEAFLSEIALLTEISAETVSEGGEPDEKMILSSVHQAKGLEWRAVFVVWLADGRFPSAQA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHH
LKDTDGEEEERRLFYVASTRAKDELYLTYPVVAAPRDRERVVMKASRFLEELPAEPELFE
HCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHH
RWQLDDPGLPAALGAAPPPAAGLPPRDPSVVPALFGEPAPRRGPADVVAPVEADGGDPGG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCC
GDDVPF
CCCCCC
>Mature Secondary Structure 
SRHYQLKAASPPARALNYQGLLNDQQIAVVEAGDGPILVIAGAGSGKTRTLTWRVARLV
CCCEEEECCCCCHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHH
ADGVAPEGILLLTFTNKAAREMLRRVEEVCRVDTRRISGGTFHHVAHEVLREHAAELGYQ
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RGYSILDREDSRDVMTAAIAECGLAVGARRFPKADVLIDLVSMAVNTQTPLADVLADRRP
CCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
QFVPLTDDVLRVARRYAERKHALNAMDFDDLLLNWKILLAEREHVRRALAGRYRHVLVDE
CEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
YQDTNRLQGDVVDLIAAEHRNLCVVGDDAQSIYSFRGAHFANILEFEKRYPDARRFDLTV
HCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
NYRSTPQILSLANASIAANVRQFKKELSSVRRDGTLPAVVPCRDVQQQAAFVAQRVLELR
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHH
DEGIPLPEIAVLYRAHHHSMEIQFELARRGIPFVVRSGVRFFEAAHVKDVLAHLRFARNP
HCCCCCHHHHHHHHHHHCCEEEEEHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCC
GDELALKRCLKITPGVGTATADAVWSAFSLRRSRGRGTLDELLAPDVASHVPAKGRAGYT
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCCCCCCCCHH
RLAQLLRALGRPPTSDLPGEAIEKVLDGGYEEYLRAEFLNADQRVEDVQQLAQYARGYED
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCH
TEAFLSEIALLTEISAETVSEGGEPDEKMILSSVHQAKGLEWRAVFVVWLADGRFPSAQA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHH
LKDTDGEEEERRLFYVASTRAKDELYLTYPVVAAPRDRERVVMKASRFLEELPAEPELFE
HCCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHH
RWQLDDPGLPAALGAAPPPAAGLPPRDPSVVPALFGEPAPRRGPADVVAPVEADGGDPGG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCC
GDDVPF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]